Slackia isoflavoniconvertens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Slackia

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1842 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N0IKJ9|A0A3N0IKJ9_9ACTN Laccase domain-containing protein OS=Slackia isoflavoniconvertens OX=572010 GN=DMP05_00785 PE=3 SV=1
MM1 pKa = 7.89AEE3 pKa = 4.27DD4 pKa = 4.07HH5 pKa = 6.96TNQQPQQPPQPAEE18 pKa = 3.75PQGFDD23 pKa = 3.16AQQSQAYY30 pKa = 9.25AQGQAQPFAQSQPYY44 pKa = 9.44GQAQAQQPYY53 pKa = 8.57GQPGAHH59 pKa = 6.17QYY61 pKa = 9.35AQPGCGQQYY70 pKa = 8.55YY71 pKa = 10.52APAAQPVYY79 pKa = 10.33AAPASRR85 pKa = 11.84KK86 pKa = 10.17KK87 pKa = 10.9NMTPWIVLGVILFAVVFCFTIGAMSCSSAVRR118 pKa = 11.84SLSNFGMGSVEE129 pKa = 4.56EE130 pKa = 4.47EE131 pKa = 4.26LTDD134 pKa = 4.16GPTVAVIDD142 pKa = 3.59ISGTIQYY149 pKa = 10.52DD150 pKa = 3.61GTACSPEE157 pKa = 4.22GLSSLLNQAEE167 pKa = 4.2EE168 pKa = 4.14NDD170 pKa = 4.31DD171 pKa = 3.54IEE173 pKa = 5.03AVVLRR178 pKa = 11.84VDD180 pKa = 3.68SGGGTATAGEE190 pKa = 4.23EE191 pKa = 3.52MAEE194 pKa = 4.08YY195 pKa = 10.52VKK197 pKa = 10.63RR198 pKa = 11.84FSKK201 pKa = 10.57PVVVSSASMNCSAAYY216 pKa = 9.24EE217 pKa = 4.16ISSQADD223 pKa = 4.13LIYY226 pKa = 11.03VNKK229 pKa = 8.02TTDD232 pKa = 2.49IGAIGTVMQVTDD244 pKa = 4.74LSEE247 pKa = 4.87LLDD250 pKa = 3.7KK251 pKa = 11.43LGISVDD257 pKa = 4.01NIASAEE263 pKa = 4.37SKK265 pKa = 10.42DD266 pKa = 3.43SSYY269 pKa = 10.19GTRR272 pKa = 11.84PLTDD276 pKa = 3.21EE277 pKa = 3.65EE278 pKa = 3.88RR279 pKa = 11.84AYY281 pKa = 10.39YY282 pKa = 9.96QEE284 pKa = 5.68LIDD287 pKa = 3.64VTNEE291 pKa = 3.63VFVEE295 pKa = 4.52NVAEE299 pKa = 4.3GRR301 pKa = 11.84SMSLDD306 pKa = 3.34EE307 pKa = 4.91ARR309 pKa = 11.84ALATGLSFTGEE320 pKa = 3.9QAVEE324 pKa = 3.85NGLADD329 pKa = 3.71EE330 pKa = 4.81VGVFDD335 pKa = 5.87DD336 pKa = 5.48AIEE339 pKa = 4.33AAAQLAGLGSDD350 pKa = 3.82YY351 pKa = 11.56NVITLTQSSDD361 pKa = 3.13LGSLMDD367 pKa = 5.33LLSEE371 pKa = 4.41SDD373 pKa = 3.53SDD375 pKa = 4.24SATSAAAAKK384 pKa = 10.38LIEE387 pKa = 4.07LLKK390 pKa = 11.16NEE392 pKa = 4.46GVLSS396 pKa = 3.83

Molecular weight: 41.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369LL14|A0A369LL14_9ACTN Protein RecA OS=Slackia isoflavoniconvertens OX=572010 GN=recA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.35QPNNRR10 pKa = 11.84KK11 pKa = 8.98RR12 pKa = 11.84AKK14 pKa = 8.49THH16 pKa = 5.22GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27GGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1842

0

1842

628285

37

5068

341.1

37.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.74 ± 0.089

1.696 ± 0.027

6.018 ± 0.047

6.814 ± 0.053

3.906 ± 0.042

8.124 ± 0.053

1.896 ± 0.025

5.364 ± 0.043

4.411 ± 0.047

8.508 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.03

3.216 ± 0.043

4.07 ± 0.032

2.868 ± 0.03

5.529 ± 0.081

6.101 ± 0.05

5.201 ± 0.07

7.941 ± 0.055

1.034 ± 0.02

2.798 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski