Streptococcus phage Javan253

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A679|A0A4D6A679_9CAUD Resolvase OS=Streptococcus phage Javan253 OX=2548073 GN=Javan253_0023 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.56FEE4 pKa = 5.57LKK6 pKa = 10.85NKK8 pKa = 9.72DD9 pKa = 3.81LNSFLQTIDD18 pKa = 3.49KK19 pKa = 9.1LTVSSMRR26 pKa = 11.84VNRR29 pKa = 11.84GKK31 pKa = 10.79AKK33 pKa = 10.02VYY35 pKa = 10.64SAISDD40 pKa = 3.58KK41 pKa = 11.26LEE43 pKa = 4.03EE44 pKa = 4.14YY45 pKa = 10.93GRR47 pKa = 11.84DD48 pKa = 3.2EE49 pKa = 5.45GEE51 pKa = 3.74ILEE54 pKa = 4.46KK55 pKa = 11.04NIVTNEE61 pKa = 3.49YY62 pKa = 8.59GQLMKK67 pKa = 10.88EE68 pKa = 4.17EE69 pKa = 4.49NGDD72 pKa = 4.11FILKK76 pKa = 10.2EE77 pKa = 3.93NVTITEE83 pKa = 4.16INQQLSEE90 pKa = 4.18LQEE93 pKa = 3.85EE94 pKa = 5.24AITISSGDD102 pKa = 3.45YY103 pKa = 9.92TNRR106 pKa = 11.84FTDD109 pKa = 4.13FFDD112 pKa = 3.35WLSDD116 pKa = 3.69CEE118 pKa = 4.48EE119 pKa = 4.3EE120 pKa = 4.17FTTAEE125 pKa = 4.3VILIDD130 pKa = 3.66NLLEE134 pKa = 3.87QFEE137 pKa = 4.39AQKK140 pKa = 11.49GEE142 pKa = 3.92

Molecular weight: 16.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A803|A0A4D6A803_9CAUD Uncharacterized protein OS=Streptococcus phage Javan253 OX=2548073 GN=Javan253_0031 PE=4 SV=1
MM1 pKa = 7.42FFLRR5 pKa = 11.84QKK7 pKa = 10.41SKK9 pKa = 11.09RR10 pKa = 11.84KK11 pKa = 9.73GKK13 pKa = 10.16GAVSSAPLSFVSSLTKK29 pKa = 10.85AHH31 pKa = 5.95MEE33 pKa = 4.12KK34 pKa = 10.56KK35 pKa = 10.36NEE37 pKa = 3.97RR38 pKa = 11.84FF39 pKa = 3.33

Molecular weight: 4.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12114

37

1463

205.3

23.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.413 ± 0.373

0.57 ± 0.095

6.538 ± 0.248

7.743 ± 0.486

3.83 ± 0.218

5.952 ± 0.408

1.445 ± 0.161

7.074 ± 0.347

8.503 ± 0.277

8.238 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.212

5.374 ± 0.254

2.93 ± 0.338

4.425 ± 0.276

3.987 ± 0.249

6.067 ± 0.399

6.142 ± 0.514

6.043 ± 0.26

1.23 ± 0.177

3.88 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski