Capybara microvirus Cap3_SP_472
Average proteome isoelectric point is 5.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8Q4|A0A4P8W8Q4_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_472 OX=2585466 PE=4 SV=1
MM1 pKa = 7.61 NEE3 pKa = 3.58 TLLYY7 pKa = 7.65 RR8 pKa = 11.84 TPYY11 pKa = 10.05 DD12 pKa = 3.3 GKK14 pKa = 9.11 TSRR17 pKa = 11.84 IPTDD21 pKa = 4.33 PGSEE25 pKa = 3.67 WANEE29 pKa = 3.91 YY30 pKa = 10.38 EE31 pKa = 4.07 LQIVNKK37 pKa = 10.23 KK38 pKa = 9.91 KK39 pKa = 10.49 DD40 pKa = 3.37 LVYY43 pKa = 10.24 TGRR46 pKa = 11.84 HH47 pKa = 4.7 NLYY50 pKa = 10.87 EE51 pKa = 4.49 EE52 pKa = 3.69 IQKK55 pKa = 10.65 YY56 pKa = 9.45 KK57 pKa = 10.52 EE58 pKa = 3.68 EE59 pKa = 4.48 CEE61 pKa = 3.75 IEE63 pKa = 4.71 NILKK67 pKa = 10.54 RR68 pKa = 11.84 MSTGDD73 pKa = 3.38 YY74 pKa = 11.31 SMLGEE79 pKa = 4.4 EE80 pKa = 4.84 GNYY83 pKa = 9.55 IDD85 pKa = 5.14 TEE87 pKa = 4.3 GMPDD91 pKa = 3.72 NLMDD95 pKa = 4.22 AQNSILKK102 pKa = 9.29 LQNEE106 pKa = 4.35 WLKK109 pKa = 11.32 LPVEE113 pKa = 4.05 IRR115 pKa = 11.84 KK116 pKa = 9.78 KK117 pKa = 10.58 FDD119 pKa = 3.16 NSLEE123 pKa = 4.11 AFVEE127 pKa = 4.54 EE128 pKa = 4.64 SGSEE132 pKa = 3.61 EE133 pKa = 3.99 WLNKK137 pKa = 10.12 LGYY140 pKa = 6.72 EE141 pKa = 4.27 TKK143 pKa = 10.38 KK144 pKa = 10.81 EE145 pKa = 3.93 EE146 pKa = 4.22 TMKK149 pKa = 9.55 QTSTEE154 pKa = 3.95 TGEE157 pKa = 4.2 TNEE160 pKa = 4.07
Molecular weight: 18.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.635
IPC2_protein 4.622
IPC_protein 4.507
Toseland 4.368
ProMoST 4.546
Dawson 4.431
Bjellqvist 4.609
Wikipedia 4.253
Rodwell 4.342
Grimsley 4.279
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.609
Thurlkill 4.355
EMBOSS 4.279
Sillero 4.609
Patrickios 3.859
IPC_peptide 4.431
IPC2_peptide 4.609
IPC2.peptide.svr19 4.53
Protein with the highest isoelectric point:
>tr|A0A4P8W624|A0A4P8W624_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_472 OX=2585466 PE=4 SV=1
MM1 pKa = 7.41 GCKK4 pKa = 9.4 KK5 pKa = 10.31 PKK7 pKa = 10.28 IIVQDD12 pKa = 3.74 LNKK15 pKa = 9.82 WKK17 pKa = 8.97 TAADD21 pKa = 3.29 GHH23 pKa = 6.42 KK24 pKa = 10.17 YY25 pKa = 10.02 HH26 pKa = 7.25 PAEE29 pKa = 4.17 CNYY32 pKa = 10.76 ASEE35 pKa = 4.54 EE36 pKa = 4.59 DD37 pKa = 3.41 INTYY41 pKa = 10.35 KK42 pKa = 10.71 DD43 pKa = 3.47 EE44 pKa = 4.68 KK45 pKa = 10.53 IKK47 pKa = 10.75 IIGTHH52 pKa = 6.33 KK53 pKa = 10.7 YY54 pKa = 10.62 SLVPCGQCLGCRR66 pKa = 11.84 LQRR69 pKa = 11.84 SAEE72 pKa = 3.89 WADD75 pKa = 3.57 RR76 pKa = 11.84 CTLEE80 pKa = 4.2 MEE82 pKa = 4.79 EE83 pKa = 5.17 NPEE86 pKa = 4.03 QCWFLTLTYY95 pKa = 10.6 NDD97 pKa = 4.03 EE98 pKa = 4.15 NLPYY102 pKa = 9.66 IDD104 pKa = 5.11 EE105 pKa = 4.39 FTTKK109 pKa = 10.44 KK110 pKa = 10.73 GITYY114 pKa = 9.62 TDD116 pKa = 3.76 TEE118 pKa = 4.66 GTWNGCLQKK127 pKa = 10.68 KK128 pKa = 10.03 DD129 pKa = 3.24 IQDD132 pKa = 3.2 FLKK135 pKa = 10.32 RR136 pKa = 11.84 LRR138 pKa = 11.84 RR139 pKa = 11.84 RR140 pKa = 11.84 WEE142 pKa = 3.96 YY143 pKa = 10.84 KK144 pKa = 10.29 YY145 pKa = 10.27 SAHH148 pKa = 7.27 NIRR151 pKa = 11.84 TFYY154 pKa = 10.45 CGEE157 pKa = 4.06 YY158 pKa = 10.48 GPQTEE163 pKa = 4.19 RR164 pKa = 11.84 PHH166 pKa = 7.48 YY167 pKa = 10.91 HH168 pKa = 6.4 MICFNLPITPDD179 pKa = 2.71 MMTIQKK185 pKa = 10.16 LNWEE189 pKa = 3.96 KK190 pKa = 11.08 DD191 pKa = 3.01 ILYY194 pKa = 9.96 KK195 pKa = 10.41 CDD197 pKa = 4.07 EE198 pKa = 4.66 IEE200 pKa = 4.25 QIWGKK205 pKa = 9.94 GFVVIEE211 pKa = 4.06 EE212 pKa = 4.97 CNWQACNYY220 pKa = 6.53 VARR223 pKa = 11.84 YY224 pKa = 6.45 ITKK227 pKa = 9.9 KK228 pKa = 8.41 QTGKK232 pKa = 10.52 LADD235 pKa = 3.61 EE236 pKa = 5.03 YY237 pKa = 10.88 YY238 pKa = 10.3 AQKK241 pKa = 10.79 GQIPPFVQASLKK253 pKa = 9.94 PAIGKK258 pKa = 9.52 NYY260 pKa = 8.41 YY261 pKa = 9.46 QEE263 pKa = 4.33 NKK265 pKa = 10.22 SKK267 pKa = 10.26 IYY269 pKa = 10.63 EE270 pKa = 4.08 NDD272 pKa = 3.26 NIIITGKK279 pKa = 10.81 GKK281 pKa = 9.44 VLKK284 pKa = 9.94 IKK286 pKa = 9.9 PPRR289 pKa = 11.84 YY290 pKa = 9.0 FDD292 pKa = 3.42 KK293 pKa = 11.02 LYY295 pKa = 11.13 DD296 pKa = 3.92 LDD298 pKa = 4.41 YY299 pKa = 10.52 PSDD302 pKa = 3.38 MLRR305 pKa = 11.84 IKK307 pKa = 10.5 KK308 pKa = 9.86 LRR310 pKa = 11.84 EE311 pKa = 3.5 RR312 pKa = 11.84 KK313 pKa = 9.85 AMNGLLLKK321 pKa = 8.72 TQNTSLDD328 pKa = 3.55 YY329 pKa = 11.38 LEE331 pKa = 4.02 QLKK334 pKa = 10.36 IEE336 pKa = 4.22 EE337 pKa = 5.04 DD338 pKa = 4.1 YY339 pKa = 10.58 LTEE342 pKa = 3.87 RR343 pKa = 11.84 TTTLKK348 pKa = 9.76 RR349 pKa = 11.84 TNAEE353 pKa = 3.81 AA354 pKa = 3.93
Molecular weight: 41.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.111
IPC2_protein 7.454
IPC_protein 7.278
Toseland 6.854
ProMoST 7.878
Dawson 8.009
Bjellqvist 8.39
Wikipedia 7.922
Rodwell 8.024
Grimsley 6.868
Solomon 8.053
Lehninger 8.068
Nozaki 8.58
DTASelect 8.112
Thurlkill 8.17
EMBOSS 8.214
Sillero 8.507
Patrickios 3.935
IPC_peptide 8.039
IPC2_peptide 7.337
IPC2.peptide.svr19 7.551
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1536
88
571
256.0
29.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.641 ± 1.872
1.432 ± 0.728
4.883 ± 0.65
8.398 ± 1.773
2.539 ± 0.548
6.641 ± 1.057
1.628 ± 0.396
7.096 ± 1.281
7.422 ± 1.673
6.51 ± 0.837
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.734 ± 0.364
6.576 ± 0.911
3.711 ± 1.087
4.557 ± 0.501
3.776 ± 0.345
7.747 ± 3.347
6.706 ± 0.868
3.19 ± 1.06
2.279 ± 0.225
5.534 ± 0.674
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here