Pasteurellaceae bacterium RH1A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; unclassified Pasteurellaceae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2012 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H0T9Y7|A0A6H0T9Y7_9PAST Uncharacterized protein OS=Pasteurellaceae bacterium RH1A OX=1823760 GN=A4G20_06555 PE=4 SV=1
MM1 pKa = 7.33AVVGLFYY8 pKa = 11.0GSDD11 pKa = 3.2TGNTEE16 pKa = 4.52NISKK20 pKa = 9.68MIQKK24 pKa = 9.58QLGEE28 pKa = 4.12NLIDD32 pKa = 3.45IRR34 pKa = 11.84DD35 pKa = 3.59IAKK38 pKa = 8.48STKK41 pKa = 9.86EE42 pKa = 4.18DD43 pKa = 3.05IEE45 pKa = 5.8AYY47 pKa = 10.34DD48 pKa = 4.09FLLLGIPTWYY58 pKa = 10.3YY59 pKa = 11.42GEE61 pKa = 4.66SQADD65 pKa = 3.34WDD67 pKa = 4.88DD68 pKa = 4.41FMPTLKK74 pKa = 10.75EE75 pKa = 3.45IDD77 pKa = 3.73FSGKK81 pKa = 9.07IVGIFGCGDD90 pKa = 3.21QEE92 pKa = 5.04DD93 pKa = 4.3YY94 pKa = 11.79AEE96 pKa = 4.32YY97 pKa = 10.5FCDD100 pKa = 4.05AMGTVRR106 pKa = 11.84DD107 pKa = 3.69IVEE110 pKa = 4.23PNGGIIVGHH119 pKa = 6.97WPTEE123 pKa = 4.24GYY125 pKa = 10.36SFEE128 pKa = 4.18VSQALVDD135 pKa = 3.81EE136 pKa = 4.45NTFVGLCIDD145 pKa = 4.19EE146 pKa = 4.82DD147 pKa = 3.95RR148 pKa = 11.84QPEE151 pKa = 4.28LTNDD155 pKa = 5.27RR156 pKa = 11.84VNQWCKK162 pKa = 10.58QIFDD166 pKa = 4.07EE167 pKa = 4.5MCLAEE172 pKa = 4.38LAA174 pKa = 4.24

Molecular weight: 19.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0T991|A0A6H0T991_9PAST Uncharacterized protein OS=Pasteurellaceae bacterium RH1A OX=1823760 GN=A4G20_07815 PE=4 SV=1
MM1 pKa = 6.91STSLEE6 pKa = 3.83AGAILRR12 pKa = 11.84SHH14 pKa = 7.14RR15 pKa = 11.84WCQRR19 pKa = 11.84PRR21 pKa = 11.84WCRR24 pKa = 11.84GLFGNLGVGKK34 pKa = 8.69QQQPGGWHH42 pKa = 6.35YY43 pKa = 10.58LTLSLMAPASSLVPWFIWKK62 pKa = 10.2SGGRR66 pKa = 11.84KK67 pKa = 8.77AATAWRR73 pKa = 11.84LALSYY78 pKa = 11.39ALIDD82 pKa = 4.06GASVLAGAVVDD93 pKa = 5.47LEE95 pKa = 4.22II96 pKa = 6.16

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2012

0

2012

633087

41

3087

314.7

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.303 ± 0.084

0.937 ± 0.019

5.127 ± 0.059

6.372 ± 0.069

4.392 ± 0.048

6.941 ± 0.076

2.008 ± 0.026

6.197 ± 0.054

6.011 ± 0.052

10.945 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.028

4.36 ± 0.052

3.914 ± 0.042

5.034 ± 0.049

4.416 ± 0.048

5.769 ± 0.041

4.913 ± 0.072

6.748 ± 0.05

1.19 ± 0.022

3.083 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski