Cladophialophora yegresii CBS 114405
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W9VYD8|W9VYD8_9EURO Uncharacterized protein OS=Cladophialophora yegresii CBS 114405 OX=1182544 GN=A1O7_07613 PE=3 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.28 NALSKK7 pKa = 10.97 AFPATAAVVALLSGQAHH24 pKa = 6.1 AQDD27 pKa = 4.59 DD28 pKa = 4.21 SGSTVINVFWITEE41 pKa = 4.2 TTLSLNSINQYY52 pKa = 10.05 PLAASIVTADD62 pKa = 3.31 SSSTVVAVEE71 pKa = 4.11 CAGPSIDD78 pKa = 3.47 TSVCGTEE85 pKa = 4.18 AQTITYY91 pKa = 10.32 GSTTWVASEE100 pKa = 4.13 TTGNLGVTMQCSIAQNSEE118 pKa = 4.13 SAVCTGEE125 pKa = 3.49 IDD127 pKa = 4.3 APEE130 pKa = 3.96 QIINDD135 pKa = 3.97 PLINSSEE142 pKa = 3.95 ASEE145 pKa = 4.89 LLTATTVDD153 pKa = 3.83 VLSTAYY159 pKa = 10.5 SGTLSSTDD167 pKa = 3.81 FTFLPITVTAGQEE180 pKa = 3.98 KK181 pKa = 10.23 LAGGSRR187 pKa = 11.84 AADD190 pKa = 3.52 SSSSSSSSGADD201 pKa = 3.0 GNANANGAGSFKK213 pKa = 11.18 SNLGLSGWMAGFLGLVMMVLL233 pKa = 4.03
Molecular weight: 23.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.554
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.037
Thurlkill 3.617
EMBOSS 3.668
Sillero 3.872
Patrickios 0.846
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.781
Protein with the highest isoelectric point:
>tr|W9WJX5|W9WJX5_9EURO RNA_pol_L_2 domain-containing protein OS=Cladophialophora yegresii CBS 114405 OX=1182544 GN=A1O7_01235 PE=3 SV=1
MM1 pKa = 7.68 AFGRR5 pKa = 11.84 SRR7 pKa = 11.84 ARR9 pKa = 11.84 HH10 pKa = 5.1 HH11 pKa = 7.01 AGGATVTTTVTTVEE25 pKa = 4.26 QPAPATRR32 pKa = 11.84 SRR34 pKa = 11.84 GTRR37 pKa = 11.84 GTRR40 pKa = 11.84 AAGTKK45 pKa = 7.27 PTLRR49 pKa = 11.84 QRR51 pKa = 11.84 LFGPPKK57 pKa = 9.35 TRR59 pKa = 11.84 TPRR62 pKa = 11.84 TTTAAPPRR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 VGTRR77 pKa = 11.84 RR78 pKa = 11.84 TPATATTTVTPRR90 pKa = 11.84 RR91 pKa = 11.84 KK92 pKa = 10.18 ASMSDD97 pKa = 3.25 KK98 pKa = 10.95 VSGAMLKK105 pKa = 10.74 LKK107 pKa = 10.67 GSLTRR112 pKa = 11.84 RR113 pKa = 11.84 PGQKK117 pKa = 9.9 AAGTRR122 pKa = 11.84 RR123 pKa = 11.84 MHH125 pKa = 5.68 GTDD128 pKa = 2.9 GRR130 pKa = 11.84 GSRR133 pKa = 11.84 RR134 pKa = 11.84 YY135 pKa = 9.15 YY136 pKa = 10.95
Molecular weight: 14.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 10.921
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.149
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.871
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10118
0
10118
4906581
43
6549
484.9
53.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.929 ± 0.019
1.144 ± 0.009
5.78 ± 0.016
6.148 ± 0.025
3.637 ± 0.015
6.941 ± 0.023
2.458 ± 0.01
4.671 ± 0.016
4.795 ± 0.021
8.858 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.151 ± 0.009
3.524 ± 0.011
6.133 ± 0.025
4.172 ± 0.017
6.258 ± 0.024
8.01 ± 0.025
6.031 ± 0.018
6.219 ± 0.018
1.434 ± 0.009
2.706 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here