Acetobacter cerevisiae
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2662 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A149VFF6|A0A149VFF6_9PROT Uncharacterized protein OS=Acetobacter cerevisiae OX=178900 GN=AD928_12610 PE=4 SV=1
MM1 pKa = 7.74 PLSALPVQPQPAPADD16 pKa = 3.46 LVFGIFNGQGQFVPQSAIWTGAVAKK41 pKa = 10.02 TGDD44 pKa = 3.97 TLSGLLSCALAPTDD58 pKa = 4.66 AAHH61 pKa = 6.98 LVNKK65 pKa = 10.09 AYY67 pKa = 11.0 VDD69 pKa = 3.67 AQSGQVSGTVATLVTQAQDD88 pKa = 3.17 AATQAQTAVGHH99 pKa = 6.6 ASDD102 pKa = 3.77 AAATIVSEE110 pKa = 4.8 QKK112 pKa = 10.77 GIPNGLATLSPDD124 pKa = 3.11 GNLVLGGLDD133 pKa = 3.41 CLGVQDD139 pKa = 4.33 GHH141 pKa = 7.69 VLMAMDD147 pKa = 6.04 LPTTDD152 pKa = 3.22 PGLRR156 pKa = 11.84 GVWWNNGGYY165 pKa = 10.35 LCISQGTSSS174 pKa = 3.29
Molecular weight: 17.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.84
IPC2_protein 4.113
IPC_protein 4.062
Toseland 3.834
ProMoST 4.266
Dawson 4.088
Bjellqvist 4.253
Wikipedia 4.101
Rodwell 3.897
Grimsley 3.745
Solomon 4.075
Lehninger 4.037
Nozaki 4.215
DTASelect 4.546
Thurlkill 3.923
EMBOSS 4.101
Sillero 4.202
Patrickios 1.99
IPC_peptide 4.062
IPC2_peptide 4.164
IPC2.peptide.svr19 4.061
Protein with the highest isoelectric point:
>tr|A0A149V9J4|A0A149V9J4_9PROT CTP synthase OS=Acetobacter cerevisiae OX=178900 GN=pyrG PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.25 IIANRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.47 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2662
0
2662
864380
26
2852
324.7
35.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.802 ± 0.07
1.025 ± 0.015
5.396 ± 0.036
5.251 ± 0.048
3.566 ± 0.031
8.308 ± 0.052
2.389 ± 0.025
4.668 ± 0.035
3.352 ± 0.039
10.428 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.541 ± 0.023
2.737 ± 0.037
5.609 ± 0.043
3.752 ± 0.032
6.605 ± 0.045
5.762 ± 0.035
5.932 ± 0.043
7.172 ± 0.048
1.401 ± 0.017
2.304 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here