Nootka lupine vein clearing virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus

Average proteome isoelectric point is 8.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A2TJS9|A2TJS9_9TOMB RNA-directed RNA polymerase (Fragment) OS=Nootka lupine vein clearing virus OX=283876 PE=4 SV=1
MM1 pKa = 7.49GVFSKK6 pKa = 10.56LAKK9 pKa = 9.92EE10 pKa = 3.92LVIGSGTLGVLGCCLYY26 pKa = 10.46AAAVVRR32 pKa = 11.84VTVGTVEE39 pKa = 4.11FGIAAVNTVSDD50 pKa = 4.72FVQSGGLSIPTVPQYY65 pKa = 11.36PNLGYY70 pKa = 10.71SPFQVEE76 pKa = 4.83LDD78 pKa = 3.54QSAGKK83 pKa = 9.5EE84 pKa = 3.95DD85 pKa = 3.95EE86 pKa = 5.08GISMLEE92 pKa = 3.67ASTLEE97 pKa = 4.2EE98 pKa = 4.09KK99 pKa = 10.85KK100 pKa = 10.56EE101 pKa = 3.79ADD103 pKa = 3.54SEE105 pKa = 4.54GKK107 pKa = 9.07MVVVSRR113 pKa = 11.84KK114 pKa = 9.36RR115 pKa = 11.84KK116 pKa = 8.72VNRR119 pKa = 11.84HH120 pKa = 4.35QKK122 pKa = 9.16GQFVMDD128 pKa = 3.76VVRR131 pKa = 11.84KK132 pKa = 9.8AKK134 pKa = 9.28MHH136 pKa = 6.37FGCTPSPTRR145 pKa = 11.84ANEE148 pKa = 3.83LAVAKK153 pKa = 10.04YY154 pKa = 10.21VAGLCKK160 pKa = 9.81EE161 pKa = 3.92QHH163 pKa = 6.12LVASHH168 pKa = 5.71ARR170 pKa = 11.84EE171 pKa = 3.92VTEE174 pKa = 3.93VAKK177 pKa = 10.97ALVFTPDD184 pKa = 2.77IAEE187 pKa = 4.01IQGTKK192 pKa = 10.46LLNSYY197 pKa = 9.55RR198 pKa = 11.84AYY200 pKa = 10.09ARR202 pKa = 11.84RR203 pKa = 11.84VALHH207 pKa = 7.1DD208 pKa = 4.19AQQIDD213 pKa = 4.27QWWLNLVKK221 pKa = 10.65HH222 pKa = 5.99PLSTNSWAEE231 pKa = 3.11AWYY234 pKa = 8.41TLNGAPPRR242 pKa = 11.84AAVQFNKK249 pKa = 10.66

Molecular weight: 27.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A2TJT2|A2TJT2_9TOMB Capsid protein OS=Nootka lupine vein clearing virus OX=283876 PE=3 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84LWIKK6 pKa = 8.87MHH8 pKa = 6.15LRR10 pKa = 11.84VIVGVVTFLLLTRR23 pKa = 11.84WKK25 pKa = 9.99FAIRR29 pKa = 11.84SFSISEE35 pKa = 4.0ILPPLITLNQIITLSFLILFVNCLCRR61 pKa = 11.84GGDD64 pKa = 3.91TIFLDD69 pKa = 3.88PLHH72 pKa = 6.71RR73 pKa = 5.4

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1259

67

525

251.8

27.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.546 ± 1.444

1.986 ± 0.678

4.289 ± 0.444

5.481 ± 1.078

4.289 ± 0.706

8.181 ± 0.747

2.224 ± 0.284

4.686 ± 1.564

5.957 ± 1.143

7.466 ± 2.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.78 ± 0.253

4.21 ± 0.342

4.051 ± 0.129

3.892 ± 0.64

6.593 ± 0.739

7.069 ± 1.503

5.878 ± 1.234

9.373 ± 1.013

1.35 ± 0.251

2.701 ± 0.674
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski