Siphoviridae sp. ctdEk19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W955|A0A5Q2W955_9CAUD Uncharacterized protein OS=Siphoviridae sp. ctdEk19 OX=2656720 PE=4 SV=1
MM1 pKa = 7.53AFDD4 pKa = 5.08GNGTYY9 pKa = 10.81NLPTPEE15 pKa = 4.09YY16 pKa = 8.43PALPGTLIEE25 pKa = 4.33SASYY29 pKa = 10.81NAVLEE34 pKa = 4.45DD35 pKa = 3.99LATALSTCMVRR46 pKa = 11.84DD47 pKa = 4.08GQATALGNWNMGGYY61 pKa = 10.18KK62 pKa = 9.94LQNLANGVTAQDD74 pKa = 3.38AVTFSQVFTSPSFSNVTATGATFTVTATAANLSAVTTCTLPSGTSIGSVDD124 pKa = 3.75ASEE127 pKa = 4.18LAYY130 pKa = 11.2LNGVTSAIQTQFTVEE145 pKa = 3.79TAARR149 pKa = 11.84IAGDD153 pKa = 3.51ALKK156 pKa = 11.09ANLAGGNTFTGTQNLVAALTNGSVAATQVSTDD188 pKa = 2.9SSTLVATTAFVQSVAFSAALPNQAGNNGSFVTTNGTTASWQRR230 pKa = 11.84IVAGDD235 pKa = 4.39FINLALGVLL244 pKa = 4.07

Molecular weight: 24.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WAB6|A0A5Q2WAB6_9CAUD Uncharacterized protein OS=Siphoviridae sp. ctdEk19 OX=2656720 PE=4 SV=1
MM1 pKa = 7.48SWPPSTGHH9 pKa = 6.43RR10 pKa = 11.84QLTFPKK16 pKa = 10.08RR17 pKa = 11.84KK18 pKa = 9.55LPQARR23 pKa = 11.84PLTRR27 pKa = 11.84RR28 pKa = 11.84MKK30 pKa = 10.41NGSITVPRR38 pKa = 11.84MSRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SAKK46 pKa = 8.9TGAVPPPLL54 pKa = 4.84

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

10929

29

795

198.7

21.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.014 ± 0.645

1.171 ± 0.175

4.813 ± 0.214

6.112 ± 0.388

3.321 ± 0.199

8.692 ± 0.768

1.574 ± 0.144

3.514 ± 0.317

4.072 ± 0.342

9.589 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.145

3.212 ± 0.254

5.646 ± 0.305

5.124 ± 0.381

6.551 ± 0.375

5.444 ± 0.365

5.801 ± 0.365

6.579 ± 0.338

1.812 ± 0.195

2.58 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski