Amino acid dipepetide frequency for Siphoviridae sp. ctdEk19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.55AlaAla: 14.55 ± 2.014
0.915AlaCys: 0.915 ± 0.261
5.033AlaAsp: 5.033 ± 0.849
8.236AlaGlu: 8.236 ± 1.032
2.837AlaPhe: 2.837 ± 0.47
12.354AlaGly: 12.354 ± 2.767
1.556AlaHis: 1.556 ± 0.434
5.216AlaIle: 5.216 ± 0.756
5.673AlaLys: 5.673 ± 1.005
11.896AlaLeu: 11.896 ± 1.033
3.477AlaMet: 3.477 ± 0.374
3.477AlaAsn: 3.477 ± 0.771
4.758AlaPro: 4.758 ± 0.595
6.04AlaGln: 6.04 ± 0.858
7.687AlaArg: 7.687 ± 0.927
6.223AlaSer: 6.223 ± 0.791
7.504AlaThr: 7.504 ± 1.188
9.151AlaVal: 9.151 ± 1.209
1.556AlaTrp: 1.556 ± 0.335
2.013AlaTyr: 2.013 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.549CysAla: 0.549 ± 0.221
0.549CysCys: 0.549 ± 0.211
0.732CysAsp: 0.732 ± 0.281
0.732CysGlu: 0.732 ± 0.303
0.183CysPhe: 0.183 ± 0.121
1.464CysGly: 1.464 ± 0.34
0.183CysHis: 0.183 ± 0.121
0.275CysIle: 0.275 ± 0.186
0.549CysLys: 0.549 ± 0.226
0.641CysLeu: 0.641 ± 0.265
0.366CysMet: 0.366 ± 0.162
0.458CysAsn: 0.458 ± 0.191
1.098CysPro: 1.098 ± 0.345
0.366CysGln: 0.366 ± 0.164
1.373CysArg: 1.373 ± 0.409
0.732CysSer: 0.732 ± 0.246
0.732CysThr: 0.732 ± 0.262
0.458CysVal: 0.458 ± 0.169
0.092CysTrp: 0.092 ± 0.092
0.183CysTyr: 0.183 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
6.04AspAla: 6.04 ± 0.842
0.641AspCys: 0.641 ± 0.25
3.02AspAsp: 3.02 ± 0.417
4.118AspGlu: 4.118 ± 0.598
1.83AspPhe: 1.83 ± 0.352
3.294AspGly: 3.294 ± 0.448
0.549AspHis: 0.549 ± 0.224
1.556AspIle: 1.556 ± 0.296
1.281AspLys: 1.281 ± 0.401
5.216AspLeu: 5.216 ± 0.579
1.19AspMet: 1.19 ± 0.373
1.464AspAsn: 1.464 ± 0.363
4.758AspPro: 4.758 ± 0.546
1.464AspGln: 1.464 ± 0.444
2.013AspArg: 2.013 ± 0.527
2.288AspSer: 2.288 ± 0.402
2.562AspThr: 2.562 ± 0.495
2.562AspVal: 2.562 ± 0.437
1.373AspTrp: 1.373 ± 0.365
0.915AspTyr: 0.915 ± 0.326
0.0AspXaa: 0.0 ± 0.0
Glu
7.687GluAla: 7.687 ± 1.148
0.549GluCys: 0.549 ± 0.289
2.745GluAsp: 2.745 ± 0.507
3.569GluGlu: 3.569 ± 0.843
2.288GluPhe: 2.288 ± 0.55
5.124GluGly: 5.124 ± 0.636
0.549GluHis: 0.549 ± 0.209
2.196GluIle: 2.196 ± 0.418
2.379GluLys: 2.379 ± 0.49
7.595GluLeu: 7.595 ± 0.981
1.922GluMet: 1.922 ± 0.354
1.007GluAsn: 1.007 ± 0.272
1.922GluPro: 1.922 ± 0.39
4.575GluGln: 4.575 ± 0.626
4.575GluArg: 4.575 ± 0.626
2.105GluSer: 2.105 ± 0.438
3.935GluThr: 3.935 ± 0.706
3.752GluVal: 3.752 ± 0.687
1.19GluTrp: 1.19 ± 0.418
1.373GluTyr: 1.373 ± 0.315
0.0GluXaa: 0.0 ± 0.0
Phe
3.843PheAla: 3.843 ± 0.701
0.458PheCys: 0.458 ± 0.18
2.196PheAsp: 2.196 ± 0.374
2.654PheGlu: 2.654 ± 0.439
1.281PhePhe: 1.281 ± 0.334
3.111PheGly: 3.111 ± 0.684
0.092PheHis: 0.092 ± 0.096
1.739PheIle: 1.739 ± 0.435
1.281PheLys: 1.281 ± 0.264
2.196PheLeu: 2.196 ± 0.472
0.641PheMet: 0.641 ± 0.235
0.915PheAsn: 0.915 ± 0.256
2.471PhePro: 2.471 ± 0.492
1.739PheGln: 1.739 ± 0.349
1.922PheArg: 1.922 ± 0.459
1.647PheSer: 1.647 ± 0.365
2.471PheThr: 2.471 ± 0.566
1.098PheVal: 1.098 ± 0.305
0.458PheTrp: 0.458 ± 0.21
1.007PheTyr: 1.007 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
9.151GlyAla: 9.151 ± 2.051
0.824GlyCys: 0.824 ± 0.331
3.203GlyAsp: 3.203 ± 0.631
5.216GlyGlu: 5.216 ± 0.798
2.471GlyPhe: 2.471 ± 0.436
12.171GlyGly: 12.171 ± 4.492
2.288GlyHis: 2.288 ± 0.406
2.837GlyIle: 2.837 ± 0.374
4.026GlyLys: 4.026 ± 0.726
7.229GlyLeu: 7.229 ± 0.725
2.379GlyMet: 2.379 ± 0.502
2.654GlyAsn: 2.654 ± 0.626
2.562GlyPro: 2.562 ± 0.425
4.484GlyGln: 4.484 ± 0.666
4.85GlyArg: 4.85 ± 0.668
5.765GlySer: 5.765 ± 0.973
4.758GlyThr: 4.758 ± 0.717
5.49GlyVal: 5.49 ± 0.719
1.647GlyTrp: 1.647 ± 0.289
2.928GlyTyr: 2.928 ± 0.396
0.0GlyXaa: 0.0 ± 0.0
His
1.647HisAla: 1.647 ± 0.407
0.183HisCys: 0.183 ± 0.109
1.19HisAsp: 1.19 ± 0.374
0.641HisGlu: 0.641 ± 0.222
1.19HisPhe: 1.19 ± 0.278
1.373HisGly: 1.373 ± 0.387
0.275HisHis: 0.275 ± 0.182
0.732HisIle: 0.732 ± 0.227
0.366HisLys: 0.366 ± 0.171
1.922HisLeu: 1.922 ± 0.395
0.275HisMet: 0.275 ± 0.169
0.366HisAsn: 0.366 ± 0.187
0.824HisPro: 0.824 ± 0.298
1.007HisGln: 1.007 ± 0.299
1.007HisArg: 1.007 ± 0.318
0.458HisSer: 0.458 ± 0.205
0.732HisThr: 0.732 ± 0.198
0.732HisVal: 0.732 ± 0.206
0.458HisTrp: 0.458 ± 0.197
0.366HisTyr: 0.366 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
5.124IleAla: 5.124 ± 0.788
0.824IleCys: 0.824 ± 0.257
2.013IleAsp: 2.013 ± 0.572
2.013IleGlu: 2.013 ± 0.415
1.098IlePhe: 1.098 ± 0.386
2.654IleGly: 2.654 ± 0.482
0.458IleHis: 0.458 ± 0.214
1.007IleIle: 1.007 ± 0.36
1.373IleLys: 1.373 ± 0.457
1.647IleLeu: 1.647 ± 0.469
0.641IleMet: 0.641 ± 0.225
2.196IleAsn: 2.196 ± 0.417
2.928IlePro: 2.928 ± 0.591
1.556IleGln: 1.556 ± 0.405
2.105IleArg: 2.105 ± 0.436
1.83IleSer: 1.83 ± 0.604
2.013IleThr: 2.013 ± 0.5
2.013IleVal: 2.013 ± 0.469
0.366IleTrp: 0.366 ± 0.202
1.281IleTyr: 1.281 ± 0.293
0.0IleXaa: 0.0 ± 0.0
Lys
5.582LysAla: 5.582 ± 0.967
0.641LysCys: 0.641 ± 0.25
1.373LysAsp: 1.373 ± 0.347
2.379LysGlu: 2.379 ± 0.544
1.19LysPhe: 1.19 ± 0.259
2.745LysGly: 2.745 ± 0.407
0.458LysHis: 0.458 ± 0.246
1.098LysIle: 1.098 ± 0.267
1.19LysLys: 1.19 ± 0.324
4.85LysLeu: 4.85 ± 0.687
0.732LysMet: 0.732 ± 0.263
0.915LysAsn: 0.915 ± 0.304
2.654LysPro: 2.654 ± 0.561
2.288LysGln: 2.288 ± 0.569
3.569LysArg: 3.569 ± 0.611
2.471LysSer: 2.471 ± 0.552
1.922LysThr: 1.922 ± 0.518
3.203LysVal: 3.203 ± 0.457
0.732LysTrp: 0.732 ± 0.218
0.732LysTyr: 0.732 ± 0.304
0.0LysXaa: 0.0 ± 0.0
Leu
12.354LeuAla: 12.354 ± 0.916
0.458LeuCys: 0.458 ± 0.218
5.765LeuAsp: 5.765 ± 0.752
5.49LeuGlu: 5.49 ± 0.771
2.288LeuPhe: 2.288 ± 0.546
6.497LeuGly: 6.497 ± 0.73
1.556LeuHis: 1.556 ± 0.318
3.02LeuIle: 3.02 ± 0.465
4.026LeuLys: 4.026 ± 0.829
8.51LeuLeu: 8.51 ± 0.996
2.196LeuMet: 2.196 ± 0.399
3.386LeuAsn: 3.386 ± 0.525
6.406LeuPro: 6.406 ± 0.778
4.392LeuGln: 4.392 ± 0.843
7.321LeuArg: 7.321 ± 0.699
5.216LeuSer: 5.216 ± 0.796
5.216LeuThr: 5.216 ± 0.934
8.236LeuVal: 8.236 ± 0.994
1.281LeuTrp: 1.281 ± 0.314
2.288LeuTyr: 2.288 ± 0.414
0.0LeuXaa: 0.0 ± 0.0
Met
3.294MetAla: 3.294 ± 0.481
0.549MetCys: 0.549 ± 0.221
1.007MetAsp: 1.007 ± 0.293
1.098MetGlu: 1.098 ± 0.325
0.549MetPhe: 0.549 ± 0.198
1.373MetGly: 1.373 ± 0.335
0.092MetHis: 0.092 ± 0.096
0.641MetIle: 0.641 ± 0.313
1.19MetLys: 1.19 ± 0.352
2.288MetLeu: 2.288 ± 0.527
0.366MetMet: 0.366 ± 0.19
0.915MetAsn: 0.915 ± 0.285
1.373MetPro: 1.373 ± 0.36
1.373MetGln: 1.373 ± 0.364
1.647MetArg: 1.647 ± 0.439
1.647MetSer: 1.647 ± 0.361
1.647MetThr: 1.647 ± 0.36
1.83MetVal: 1.83 ± 0.506
0.366MetTrp: 0.366 ± 0.17
0.549MetTyr: 0.549 ± 0.254
0.0MetXaa: 0.0 ± 0.0
Asn
4.941AsnAla: 4.941 ± 0.886
0.549AsnCys: 0.549 ± 0.208
1.373AsnAsp: 1.373 ± 0.376
1.922AsnGlu: 1.922 ± 0.404
1.281AsnPhe: 1.281 ± 0.496
2.654AsnGly: 2.654 ± 0.642
0.366AsnHis: 0.366 ± 0.189
1.007AsnIle: 1.007 ± 0.331
0.915AsnLys: 0.915 ± 0.234
3.294AsnLeu: 3.294 ± 0.791
0.641AsnMet: 0.641 ± 0.282
1.19AsnAsn: 1.19 ± 0.322
1.373AsnPro: 1.373 ± 0.341
1.281AsnGln: 1.281 ± 0.55
2.013AsnArg: 2.013 ± 0.567
1.373AsnSer: 1.373 ± 0.383
1.739AsnThr: 1.739 ± 0.433
2.745AsnVal: 2.745 ± 0.509
0.915AsnTrp: 0.915 ± 0.244
0.549AsnTyr: 0.549 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
7.229ProAla: 7.229 ± 1.137
0.641ProCys: 0.641 ± 0.299
2.288ProAsp: 2.288 ± 0.423
4.575ProGlu: 4.575 ± 0.794
1.281ProPhe: 1.281 ± 0.384
3.935ProGly: 3.935 ± 0.544
0.824ProHis: 0.824 ± 0.284
2.013ProIle: 2.013 ± 0.499
2.837ProLys: 2.837 ± 0.542
5.399ProLeu: 5.399 ± 0.648
1.007ProMet: 1.007 ± 0.285
2.196ProAsn: 2.196 ± 0.481
3.843ProPro: 3.843 ± 0.825
3.203ProGln: 3.203 ± 0.617
2.562ProArg: 2.562 ± 0.464
3.477ProSer: 3.477 ± 0.608
3.569ProThr: 3.569 ± 0.552
2.928ProVal: 2.928 ± 0.505
1.098ProTrp: 1.098 ± 0.309
1.556ProTyr: 1.556 ± 0.38
0.0ProXaa: 0.0 ± 0.0
Gln
6.589GlnAla: 6.589 ± 0.904
0.183GlnCys: 0.183 ± 0.113
1.922GlnAsp: 1.922 ± 0.435
1.556GlnGlu: 1.556 ± 0.444
2.013GlnPhe: 2.013 ± 0.332
3.752GlnGly: 3.752 ± 0.693
1.281GlnHis: 1.281 ± 0.343
2.105GlnIle: 2.105 ± 0.328
2.288GlnLys: 2.288 ± 0.532
5.582GlnLeu: 5.582 ± 0.576
1.647GlnMet: 1.647 ± 0.488
1.464GlnAsn: 1.464 ± 0.325
3.294GlnPro: 3.294 ± 0.762
2.745GlnGln: 2.745 ± 0.602
2.928GlnArg: 2.928 ± 0.555
2.288GlnSer: 2.288 ± 0.407
2.288GlnThr: 2.288 ± 0.485
4.667GlnVal: 4.667 ± 0.741
0.824GlnTrp: 0.824 ± 0.291
1.83GlnTyr: 1.83 ± 0.424
0.0GlnXaa: 0.0 ± 0.0
Arg
6.314ArgAla: 6.314 ± 0.784
0.824ArgCys: 0.824 ± 0.233
2.745ArgAsp: 2.745 ± 0.524
4.667ArgGlu: 4.667 ± 0.792
3.02ArgPhe: 3.02 ± 0.691
4.575ArgGly: 4.575 ± 0.599
1.922ArgHis: 1.922 ± 0.388
2.013ArgIle: 2.013 ± 0.486
2.745ArgLys: 2.745 ± 0.531
7.412ArgLeu: 7.412 ± 1.097
1.739ArgMet: 1.739 ± 0.364
1.922ArgAsn: 1.922 ± 0.355
3.569ArgPro: 3.569 ± 0.56
3.477ArgGln: 3.477 ± 0.657
6.04ArgArg: 6.04 ± 0.86
2.654ArgSer: 2.654 ± 0.398
3.02ArgThr: 3.02 ± 0.452
4.301ArgVal: 4.301 ± 0.479
1.098ArgTrp: 1.098 ± 0.361
1.464ArgTyr: 1.464 ± 0.381
0.0ArgXaa: 0.0 ± 0.0
Ser
5.49SerAla: 5.49 ± 1.072
0.732SerCys: 0.732 ± 0.302
2.745SerAsp: 2.745 ± 0.525
2.196SerGlu: 2.196 ± 0.41
2.745SerPhe: 2.745 ± 0.546
5.582SerGly: 5.582 ± 0.724
0.275SerHis: 0.275 ± 0.154
1.556SerIle: 1.556 ± 0.338
2.196SerLys: 2.196 ± 0.561
6.131SerLeu: 6.131 ± 0.902
1.19SerMet: 1.19 ± 0.294
1.373SerAsn: 1.373 ± 0.517
3.02SerPro: 3.02 ± 0.558
2.196SerGln: 2.196 ± 0.455
3.203SerArg: 3.203 ± 0.594
4.026SerSer: 4.026 ± 0.746
3.203SerThr: 3.203 ± 0.731
2.654SerVal: 2.654 ± 0.53
1.922SerTrp: 1.922 ± 0.387
2.013SerTyr: 2.013 ± 0.359
0.0SerXaa: 0.0 ± 0.0
Thr
8.785ThrAla: 8.785 ± 1.548
0.732ThrCys: 0.732 ± 0.273
2.837ThrAsp: 2.837 ± 0.432
2.837ThrGlu: 2.837 ± 0.581
2.379ThrPhe: 2.379 ± 0.486
5.49ThrGly: 5.49 ± 0.721
0.915ThrHis: 0.915 ± 0.301
2.105ThrIle: 2.105 ± 0.549
1.83ThrLys: 1.83 ± 0.743
4.575ThrLeu: 4.575 ± 0.514
1.007ThrMet: 1.007 ± 0.317
1.647ThrAsn: 1.647 ± 0.445
3.843ThrPro: 3.843 ± 0.628
2.745ThrGln: 2.745 ± 0.545
2.837ThrArg: 2.837 ± 0.573
3.477ThrSer: 3.477 ± 0.594
4.392ThrThr: 4.392 ± 0.726
3.111ThrVal: 3.111 ± 0.71
0.824ThrTrp: 0.824 ± 0.28
1.647ThrTyr: 1.647 ± 0.379
0.0ThrXaa: 0.0 ± 0.0
Val
7.504ValAla: 7.504 ± 0.926
0.824ValCys: 0.824 ± 0.299
2.379ValAsp: 2.379 ± 0.557
4.85ValGlu: 4.85 ± 0.699
2.013ValPhe: 2.013 ± 0.449
5.582ValGly: 5.582 ± 0.79
1.464ValHis: 1.464 ± 0.399
2.288ValIle: 2.288 ± 0.556
2.562ValLys: 2.562 ± 0.536
5.673ValLeu: 5.673 ± 0.747
1.739ValMet: 1.739 ± 0.422
1.922ValAsn: 1.922 ± 0.338
3.66ValPro: 3.66 ± 0.586
3.386ValGln: 3.386 ± 0.523
4.484ValArg: 4.484 ± 0.655
3.843ValSer: 3.843 ± 0.6
4.118ValThr: 4.118 ± 0.756
4.85ValVal: 4.85 ± 0.556
0.915ValTrp: 0.915 ± 0.228
1.739ValTyr: 1.739 ± 0.519
0.0ValXaa: 0.0 ± 0.0
Trp
1.098TrpAla: 1.098 ± 0.315
0.366TrpCys: 0.366 ± 0.151
1.098TrpAsp: 1.098 ± 0.256
0.915TrpGlu: 0.915 ± 0.252
0.366TrpPhe: 0.366 ± 0.159
1.281TrpGly: 1.281 ± 0.304
0.366TrpHis: 0.366 ± 0.179
0.732TrpIle: 0.732 ± 0.286
0.824TrpLys: 0.824 ± 0.339
1.83TrpLeu: 1.83 ± 0.411
0.366TrpMet: 0.366 ± 0.179
0.641TrpAsn: 0.641 ± 0.324
0.824TrpPro: 0.824 ± 0.26
1.464TrpGln: 1.464 ± 0.306
1.83TrpArg: 1.83 ± 0.359
1.556TrpSer: 1.556 ± 0.389
0.458TrpThr: 0.458 ± 0.195
1.19TrpVal: 1.19 ± 0.282
0.275TrpTrp: 0.275 ± 0.214
0.641TrpTyr: 0.641 ± 0.265
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.379TyrAla: 2.379 ± 0.451
0.275TyrCys: 0.275 ± 0.146
2.471TyrAsp: 2.471 ± 0.419
1.464TyrGlu: 1.464 ± 0.433
0.915TyrPhe: 0.915 ± 0.305
1.922TyrGly: 1.922 ± 0.479
0.275TyrHis: 0.275 ± 0.145
1.007TyrIle: 1.007 ± 0.348
1.19TyrLys: 1.19 ± 0.297
2.013TyrLeu: 2.013 ± 0.36
0.183TyrMet: 0.183 ± 0.123
2.013TyrAsn: 2.013 ± 0.439
1.281TyrPro: 1.281 ± 0.391
1.464TyrGln: 1.464 ± 0.366
1.556TyrArg: 1.556 ± 0.436
1.373TyrSer: 1.373 ± 0.299
1.739TyrThr: 1.739 ± 0.52
0.824TyrVal: 0.824 ± 0.289
0.732TyrTrp: 0.732 ± 0.256
0.732TyrTyr: 0.732 ± 0.253
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (10929 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski