Persimmon virus A
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4WAK7|R4WAK7_9RHAB Movement protein BC1 OS=Persimmon virus A OX=1211480 PE=3 SV=1
MM1 pKa = 7.7 GDD3 pKa = 3.55 SDD5 pKa = 3.89 KK6 pKa = 11.48 VNYY9 pKa = 10.17 SGIGNPVGDD18 pKa = 4.05 YY19 pKa = 10.78 DD20 pKa = 4.56 YY21 pKa = 11.5 VGHH24 pKa = 6.93 NSGDD28 pKa = 3.41 DD29 pKa = 3.71 SYY31 pKa = 11.01 TGEE34 pKa = 4.16 HH35 pKa = 5.17 VVEE38 pKa = 4.48 SVSVPEE44 pKa = 3.97 VHH46 pKa = 6.68 NEE48 pKa = 3.35 NAVADD53 pKa = 3.68 KK54 pKa = 10.43 RR55 pKa = 11.84 ARR57 pKa = 11.84 VTPAKK62 pKa = 10.48 DD63 pKa = 3.46 VISWLSLHH71 pKa = 6.09 SKK73 pKa = 10.42 RR74 pKa = 11.84 GGLVIPVEE82 pKa = 4.22 MKK84 pKa = 10.69 NYY86 pKa = 10.07 LLSLSAEE93 pKa = 4.15 DD94 pKa = 4.47 LMEE97 pKa = 4.35 EE98 pKa = 4.2 RR99 pKa = 11.84 DD100 pKa = 3.31 VEE102 pKa = 4.47 MFVKK106 pKa = 10.6 GYY108 pKa = 8.04 VFCSSSRR115 pKa = 11.84 IIPNMQSVVNDD126 pKa = 3.27 IQAEE130 pKa = 4.22 VRR132 pKa = 11.84 NLQRR136 pKa = 11.84 EE137 pKa = 4.34 SNKK140 pKa = 10.13 NVDD143 pKa = 3.46 ILRR146 pKa = 11.84 SMEE149 pKa = 4.01 KK150 pKa = 9.8 QGKK153 pKa = 5.94 STEE156 pKa = 4.05 MEE158 pKa = 3.51 IAAAVATIRR167 pKa = 11.84 SDD169 pKa = 2.9 MVTAIKK175 pKa = 10.4 SALKK179 pKa = 9.87 EE180 pKa = 3.95 QVTATSSSSGKK191 pKa = 9.92 VEE193 pKa = 3.87 LKK195 pKa = 10.81 VRR197 pKa = 11.84 TEE199 pKa = 3.86 KK200 pKa = 10.71 QAGLDD205 pKa = 3.69 VKK207 pKa = 10.17 QVPLLINPTTSKK219 pKa = 10.86 LPRR222 pKa = 11.84 KK223 pKa = 9.09 PDD225 pKa = 3.45 LEE227 pKa = 4.07 AVRR230 pKa = 11.84 PSSSKK235 pKa = 10.12 SSKK238 pKa = 10.24 EE239 pKa = 3.68 VTLNEE244 pKa = 3.97 LKK246 pKa = 10.54 RR247 pKa = 11.84 IFMCAVGVEE256 pKa = 3.86 ADD258 pKa = 3.44 IVSVLTDD265 pKa = 3.57 EE266 pKa = 5.35 EE267 pKa = 4.36 VDD269 pKa = 4.66 AMLTANDD276 pKa = 4.01 YY277 pKa = 11.76 YY278 pKa = 10.67 EE279 pKa = 4.3 FCNDD283 pKa = 3.76 LDD285 pKa = 4.53 DD286 pKa = 5.81 DD287 pKa = 4.49 GVRR290 pKa = 11.84 KK291 pKa = 9.64 EE292 pKa = 4.04 FSEE295 pKa = 4.16 IFNKK299 pKa = 10.91 AIDD302 pKa = 3.93 QLNKK306 pKa = 9.94 EE307 pKa = 4.51 AEE309 pKa = 4.26 EE310 pKa = 4.1 EE311 pKa = 4.22 DD312 pKa = 3.65
Molecular weight: 34.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.782
IPC2_protein 4.724
IPC_protein 4.66
Toseland 4.507
ProMoST 4.724
Dawson 4.596
Bjellqvist 4.762
Wikipedia 4.469
Rodwell 4.495
Grimsley 4.418
Solomon 4.596
Lehninger 4.558
Nozaki 4.711
DTASelect 4.863
Thurlkill 4.507
EMBOSS 4.482
Sillero 4.774
Patrickios 4.164
IPC_peptide 4.609
IPC2_peptide 4.762
IPC2.peptide.svr19 4.746
Protein with the highest isoelectric point:
>tr|R4WAR4|R4WAR4_9RHAB Matrix protein OS=Persimmon virus A OX=1211480 PE=4 SV=1
MM1 pKa = 7.34 TSFVSTFKK9 pKa = 11.49 GEE11 pKa = 4.08 LNADD15 pKa = 3.48 YY16 pKa = 8.79 TQQVVKK22 pKa = 10.61 LSKK25 pKa = 10.4 KK26 pKa = 10.63 LPILQSLWISAGFDD40 pKa = 3.07 NVSITKK46 pKa = 10.09 LAFSYY51 pKa = 10.71 KK52 pKa = 9.97 SRR54 pKa = 11.84 CPKK57 pKa = 10.17 AATGVVEE64 pKa = 3.55 ITIRR68 pKa = 11.84 DD69 pKa = 3.85 LRR71 pKa = 11.84 LEE73 pKa = 4.48 DD74 pKa = 4.0 ADD76 pKa = 3.79 KK77 pKa = 11.54 QEE79 pKa = 4.18 VAFVTFNVKK88 pKa = 10.19 DD89 pKa = 3.52 WVEE92 pKa = 4.24 LSWSYY97 pKa = 10.06 PVWFHH102 pKa = 6.39 CTDD105 pKa = 3.68 FNGKK109 pKa = 8.95 HH110 pKa = 5.51 EE111 pKa = 4.81 SVLDD115 pKa = 3.53 MCLDD119 pKa = 3.74 VVDD122 pKa = 4.84 TNMSEE127 pKa = 4.05 QFSLGSYY134 pKa = 9.46 KK135 pKa = 9.93 MKK137 pKa = 10.03 IYY139 pKa = 10.81 YY140 pKa = 9.68 RR141 pKa = 11.84 VQNQITKK148 pKa = 9.58 FRR150 pKa = 11.84 PSINRR155 pKa = 11.84 AYY157 pKa = 10.84 LIDD160 pKa = 3.64 QTASPGAVITQRR172 pKa = 11.84 RR173 pKa = 11.84 KK174 pKa = 8.88 EE175 pKa = 3.89 DD176 pKa = 3.28 HH177 pKa = 6.75 NKK179 pKa = 9.92 RR180 pKa = 11.84 KK181 pKa = 10.19 SNRR184 pKa = 11.84 TSLSEE189 pKa = 4.02 EE190 pKa = 4.07 EE191 pKa = 4.29 ISIQEE196 pKa = 3.72 EE197 pKa = 4.79 VIVHH201 pKa = 6.28 KK202 pKa = 10.81 EE203 pKa = 3.88 NILDD207 pKa = 3.62 RR208 pKa = 11.84 SRR210 pKa = 11.84 SYY212 pKa = 11.18 LSSQPRR218 pKa = 11.84 QCAKK222 pKa = 10.73
Molecular weight: 25.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.738
IPC2_protein 7.936
IPC_protein 7.907
Toseland 7.966
ProMoST 8.551
Dawson 8.639
Bjellqvist 8.785
Wikipedia 8.624
Rodwell 8.697
Grimsley 7.79
Solomon 8.829
Lehninger 8.814
Nozaki 8.96
DTASelect 8.595
Thurlkill 8.697
EMBOSS 8.843
Sillero 8.946
Patrickios 4.596
IPC_peptide 8.814
IPC2_peptide 7.585
IPC2.peptide.svr19 7.712
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3821
188
2089
636.8
72.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.711 ± 0.934
1.989 ± 0.401
6.281 ± 0.307
6.229 ± 0.596
3.298 ± 0.362
5.208 ± 0.416
1.937 ± 0.371
6.569 ± 0.531
7.485 ± 0.332
9.16 ± 0.515
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.083 ± 0.59
4.632 ± 0.759
4.057 ± 0.237
2.643 ± 0.377
5.025 ± 0.451
8.898 ± 0.632
6.386 ± 0.835
6.046 ± 0.974
1.439 ± 0.267
3.926 ± 0.544
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here