Aspergillus novofumigatus IBT 16806

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus novofumigatus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I1CC97|A0A2I1CC97_ASPN1 Uncharacterized protein OS=Aspergillus novofumigatus (strain IBT 16806) OX=1392255 GN=P174DRAFT_129096 PE=4 SV=1
MM1 pKa = 7.39HH2 pKa = 7.57LALSLLALSATSTLAAPVNTLSQNQDD28 pKa = 2.64SNAPTRR34 pKa = 11.84AARR37 pKa = 11.84LLTPISHH44 pKa = 7.05PSKK47 pKa = 10.95DD48 pKa = 3.63EE49 pKa = 3.92SHH51 pKa = 6.67LKK53 pKa = 10.65ARR55 pKa = 11.84TFGLLSGLLSHH66 pKa = 6.81GFGGSGSAEE75 pKa = 3.9CEE77 pKa = 3.97ACEE80 pKa = 4.45GEE82 pKa = 4.38AGGSAGASGGLGGLLSGGLGGHH104 pKa = 7.4LGGGLGGHH112 pKa = 7.38LGGGLGLGGSAGAGEE127 pKa = 4.8DD128 pKa = 3.63CDD130 pKa = 4.19EE131 pKa = 4.55NGEE134 pKa = 4.39GGVSAGGSAGGSAGGSAGGSGEE156 pKa = 3.98GSAGGSAGGSAGGSVGGSAGGSAGGSAGGSASGSAGGDD194 pKa = 3.15TGASATGSGSAGGSAGGSAGGSATGSLGGSASGTAGGSLGGSAGGSAGGSAGGSASGSAGGDD256 pKa = 3.15TGASATGSGSTGGSATGSGGSASGSAGGDD285 pKa = 3.14TGASTTGSGSGSGSTGGSATGSVGGSGSVGGSAGGSAGGSAGGDD329 pKa = 3.09AGSSTTGSGSGSTEE343 pKa = 3.8GSGSTGGSASGSAGGDD359 pKa = 3.27TGASAPGSGSGSTEE373 pKa = 3.71GSGSGSGSGSVGGSAGGSAGGSGSSSGGISIGGSATGSGGASASATASASAGATASAGASATGGSGSDD441 pKa = 3.23NGEE444 pKa = 4.31DD445 pKa = 4.06CDD447 pKa = 4.11EE448 pKa = 4.57TGADD452 pKa = 3.83ATTGDD457 pKa = 3.78NGDD460 pKa = 4.94DD461 pKa = 3.86YY462 pKa = 11.82GDD464 pKa = 4.12DD465 pKa = 3.8AGEE468 pKa = 4.73GDD470 pKa = 4.63DD471 pKa = 5.0SSAGGDD477 pKa = 3.52DD478 pKa = 6.28SEE480 pKa = 6.76DD481 pKa = 3.84CDD483 pKa = 5.05CDD485 pKa = 3.39QQ486 pKa = 5.07

Molecular weight: 40.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I1C6A8|A0A2I1C6A8_ASPN1 Putative UBX domain protein OS=Aspergillus novofumigatus (strain IBT 16806) OX=1392255 GN=P174DRAFT_391806 PE=4 SV=1
MM1 pKa = 7.8LCFQCRR7 pKa = 11.84AVPSALRR14 pKa = 11.84TAMRR18 pKa = 11.84SQPFSRR24 pKa = 11.84LPTQTFTTSLSTPLRR39 pKa = 11.84TFSSALRR46 pKa = 11.84SQPTPLTQSLPSFRR60 pKa = 11.84TSLTPVSSLSFGLTQQTRR78 pKa = 11.84SFSASASLAGKK89 pKa = 9.59RR90 pKa = 11.84ATYY93 pKa = 10.03NPSRR97 pKa = 11.84RR98 pKa = 11.84VQKK101 pKa = 10.2RR102 pKa = 11.84RR103 pKa = 11.84HH104 pKa = 5.33GFLARR109 pKa = 11.84LRR111 pKa = 11.84SRR113 pKa = 11.84GGRR116 pKa = 11.84KK117 pKa = 8.87VLQHH121 pKa = 6.19RR122 pKa = 11.84RR123 pKa = 11.84AKK125 pKa = 9.96GRR127 pKa = 11.84KK128 pKa = 7.78SLSWW132 pKa = 3.4

Molecular weight: 14.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11478

2

11480

5199030

49

6146

452.9

50.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.602 ± 0.018

1.331 ± 0.009

5.564 ± 0.016

6.077 ± 0.022

3.753 ± 0.014

6.722 ± 0.021

2.438 ± 0.01

4.944 ± 0.014

4.575 ± 0.017

9.195 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.153 ± 0.008

3.592 ± 0.012

5.949 ± 0.026

4.02 ± 0.014

6.291 ± 0.021

8.366 ± 0.026

5.867 ± 0.014

6.222 ± 0.017

1.492 ± 0.009

2.848 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski