Coemansia reversa (strain ATCC 12441 / NRRL 1564)
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7317 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G5BIH1|A0A2G5BIH1_COERN Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase (Fragment) OS=Coemansia reversa (strain ATCC 12441 / NRRL 1564) OX=763665 GN=COEREDRAFT_22562 PE=4 SV=1
MM1 pKa = 7.71 PRR3 pKa = 11.84 PSDD6 pKa = 3.56 FTDD9 pKa = 3.47 CQARR13 pKa = 11.84 DD14 pKa = 4.14 DD15 pKa = 5.77 EE16 pKa = 4.52 IQCWIHH22 pKa = 6.23 KK23 pKa = 9.14 CHH25 pKa = 5.49 EE26 pKa = 4.37 HH27 pKa = 6.26 EE28 pKa = 6.05 DD29 pKa = 3.73 IVGEE33 pKa = 4.07 LTLDD37 pKa = 3.51 FNKK40 pKa = 9.84 VVTKK44 pKa = 10.51 RR45 pKa = 11.84 LHH47 pKa = 6.71 RR48 pKa = 11.84 DD49 pKa = 3.11 VLYY52 pKa = 10.84 HH53 pKa = 5.83 LTNGHH58 pKa = 6.17 GLGEE62 pKa = 4.19 AAALDD67 pKa = 3.49 AMVIVDD73 pKa = 3.63 SASRR77 pKa = 11.84 FLQVYY82 pKa = 8.38 PIVNQTTATLIEE94 pKa = 4.34 TLNSGWFLKK103 pKa = 11.27 YY104 pKa = 10.54 EE105 pKa = 4.68 DD106 pKa = 4.87 DD107 pKa = 4.05 TNGMGWTKK115 pKa = 9.57 FVPYY119 pKa = 9.91 IVTSYY124 pKa = 11.16 AVTVSEE130 pKa = 4.45 SSSNIPATLFFGPSAIQSDD149 pKa = 4.32 EE150 pKa = 4.17 AFALQPTGGEE160 pKa = 4.27 MPDD163 pKa = 3.32 HH164 pKa = 5.8 QQLMDD169 pKa = 4.02 HH170 pKa = 5.88 TWEE173 pKa = 4.95 PYY175 pKa = 10.12 HH176 pKa = 7.27 VVARR180 pKa = 11.84 YY181 pKa = 9.0 GPSYY185 pKa = 10.42 QLEE188 pKa = 4.34 FADD191 pKa = 4.16 GVPFVSTVAGDD202 pKa = 4.07 LLMPYY207 pKa = 9.68 HH208 pKa = 7.0 WGDD211 pKa = 3.64 DD212 pKa = 3.47 VDD214 pKa = 4.13 PAEE217 pKa = 4.45 VAPSSTDD224 pKa = 3.06 EE225 pKa = 4.6 SDD227 pKa = 3.94 PSDD230 pKa = 3.18 HH231 pKa = 7.13 SYY233 pKa = 11.51 VDD235 pKa = 3.47 EE236 pKa = 4.75 SMNDD240 pKa = 3.61 DD241 pKa = 3.66 GSSNSSNAGPSTPPPEE257 pKa = 4.25 EE258 pKa = 4.66 SNGSLYY264 pKa = 9.76 FTGSGGLGSSAALEE278 pKa = 4.33 DD279 pKa = 3.44 EE280 pKa = 4.69 CRR282 pKa = 11.84 RR283 pKa = 11.84 SPSRR287 pKa = 11.84 DD288 pKa = 2.98 TSLQLPPSFLSFVAGRR304 pKa = 11.84 DD305 pKa = 3.48 TASQSPAHH313 pKa = 6.5 ISVSSTNRR321 pKa = 11.84 SLIRR325 pKa = 11.84 GEE327 pKa = 4.03 VPDD330 pKa = 3.72 IVEE333 pKa = 4.26 VLSDD337 pKa = 3.59 SDD339 pKa = 4.03 EE340 pKa = 4.4 SDD342 pKa = 4.24 NYY344 pKa = 11.45 DD345 pKa = 3.39 SDD347 pKa = 4.04 DD348 pKa = 3.91 SEE350 pKa = 4.64 YY351 pKa = 11.25 EE352 pKa = 4.02 PLSGDD357 pKa = 3.35 NTSDD361 pKa = 3.37 KK362 pKa = 11.38 SSDD365 pKa = 3.41 EE366 pKa = 4.18 SDD368 pKa = 4.09 GKK370 pKa = 10.71 DD371 pKa = 3.08 EE372 pKa = 5.8 DD373 pKa = 4.51 EE374 pKa = 4.49 GTEE377 pKa = 4.1 PPLGPLAVSSIGFSGPSSIRR397 pKa = 11.84 NATTMSEE404 pKa = 4.11 MEE406 pKa = 4.61 TIQSTMVCDD415 pKa = 4.21 ISTAPNTCDD424 pKa = 3.05 VGVSTEE430 pKa = 3.8 NMYY433 pKa = 8.12 TTASAQTNTASDD445 pKa = 3.82 MLEE448 pKa = 3.58 QAQYY452 pKa = 11.76 GEE454 pKa = 4.54 TPTDD458 pKa = 3.22 NATMVGIEE466 pKa = 4.58 TIMVDD471 pKa = 4.52 DD472 pKa = 5.49 GDD474 pKa = 4.89 SISNDD479 pKa = 3.57 EE480 pKa = 3.77 IRR482 pKa = 11.84 IDD484 pKa = 3.37 IVEE487 pKa = 4.58 DD488 pKa = 3.8 EE489 pKa = 4.29 EE490 pKa = 5.55 DD491 pKa = 3.54 SGRR494 pKa = 11.84 SAIVPLPQVPSPQTVTTKK512 pKa = 10.97 APDD515 pKa = 3.37 NHH517 pKa = 7.09 IDD519 pKa = 3.59 AEE521 pKa = 4.55 TIPTSDD527 pKa = 3.78 NSPSEE532 pKa = 3.67 IWAMSTAIVPYY543 pKa = 8.33 SHH545 pKa = 7.01 GYY547 pKa = 8.71 YY548 pKa = 10.37 GYY550 pKa = 10.5 SPYY553 pKa = 10.93 EE554 pKa = 3.88 LAVSRR559 pKa = 11.84 ALINN563 pKa = 3.45
Molecular weight: 60.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.872
IPC_protein 3.897
Toseland 3.681
ProMoST 4.037
Dawson 3.884
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 4.012
Patrickios 1.507
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A2G5B6P2|A0A2G5B6P2_COERN Uncharacterized protein OS=Coemansia reversa (strain ATCC 12441 / NRRL 1564) OX=763665 GN=COEREDRAFT_82558 PE=4 SV=1
MM1 pKa = 6.53 TTKK4 pKa = 10.69 LPPGPRR10 pKa = 11.84 MMTTKK15 pKa = 10.59 LPPGPRR21 pKa = 11.84 MMTTKK26 pKa = 10.59 LPPGPRR32 pKa = 11.84 MMTTKK37 pKa = 10.59 LPPGPRR43 pKa = 11.84 MATTRR48 pKa = 11.84 LPPGLRR54 pKa = 11.84 MVTTKK59 pKa = 10.76 LPPGRR64 pKa = 11.84 MTVAMPVPPGPRR76 pKa = 11.84 MMTTKK81 pKa = 10.76 LPPP84 pKa = 4.06
Molecular weight: 9.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.618
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.34
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7317
0
7317
2997087
49
5170
409.6
45.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.114 ± 0.043
1.461 ± 0.013
5.695 ± 0.022
5.879 ± 0.026
3.525 ± 0.018
6.286 ± 0.023
2.524 ± 0.014
5.13 ± 0.025
4.502 ± 0.026
9.115 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.492 ± 0.012
3.988 ± 0.022
5.001 ± 0.028
4.279 ± 0.023
6.272 ± 0.03
8.233 ± 0.032
5.816 ± 0.019
6.62 ± 0.023
1.17 ± 0.01
2.899 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here