Parashewanella curva

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Parashewanella

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L8PZX5|A0A3L8PZX5_9GAMM DMT family transporter OS=Parashewanella curva OX=2338552 GN=D5018_10890 PE=4 SV=1
MM1 pKa = 7.54AKK3 pKa = 9.61IEE5 pKa = 4.51VIVGTTMGGAEE16 pKa = 4.23YY17 pKa = 10.98VSDD20 pKa = 3.83EE21 pKa = 4.81LIALLEE27 pKa = 4.08QHH29 pKa = 7.13DD30 pKa = 4.53VTQHH34 pKa = 6.49LEE36 pKa = 4.08PNLDD40 pKa = 4.37EE41 pKa = 4.92IPHH44 pKa = 5.81HH45 pKa = 6.34GIWLVVSSTHH55 pKa = 6.7GAGEE59 pKa = 4.37LPDD62 pKa = 4.73NIQPFAQQLMDD73 pKa = 3.46QSPDD77 pKa = 3.07LSQVNFAICAIGDD90 pKa = 3.55SSYY93 pKa = 11.06DD94 pKa = 3.59TFCQGPEE101 pKa = 3.48ILIQGFQQLGATAIVDD117 pKa = 4.33KK118 pKa = 11.01IQIDD122 pKa = 3.91VLDD125 pKa = 4.37EE126 pKa = 4.14NLPEE130 pKa = 4.27DD131 pKa = 3.99TAISWLEE138 pKa = 3.72SWKK141 pKa = 10.87DD142 pKa = 3.47QIAA145 pKa = 3.3

Molecular weight: 15.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L8PZD1|A0A3L8PZD1_9GAMM Uncharacterized protein OS=Parashewanella curva OX=2338552 GN=D5018_05405 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVGGRR29 pKa = 11.84KK30 pKa = 9.01VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4117

0

4117

1363167

25

2601

331.1

37.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.803 ± 0.035

1.182 ± 0.015

5.376 ± 0.029

6.253 ± 0.042

4.212 ± 0.026

6.101 ± 0.041

2.355 ± 0.02

6.512 ± 0.026

6.372 ± 0.035

10.285 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.02

4.786 ± 0.03

3.956 ± 0.026

5.014 ± 0.035

4.239 ± 0.03

7.13 ± 0.035

5.463 ± 0.026

6.457 ± 0.032

1.115 ± 0.015

3.075 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski