Streptomyces sp. 1-11
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7037 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A510TJV4|A0A510TJV4_9ACTN Uncharacterized protein OS=Streptomyces sp. 1-11 OX=2590549 GN=TNCT1_48400 PE=4 SV=1
MM1 pKa = 6.41 QQEE4 pKa = 4.22 AAGGEE9 pKa = 4.08 ALEE12 pKa = 4.16 VWIDD16 pKa = 3.49 QALCTGDD23 pKa = 4.89 GICAQYY29 pKa = 10.9 APEE32 pKa = 4.23 VFEE35 pKa = 5.9 LDD37 pKa = 3.05 IDD39 pKa = 3.65 GLAYY43 pKa = 10.7 VKK45 pKa = 10.83 GADD48 pKa = 4.66 DD49 pKa = 4.48 EE50 pKa = 4.9 LLQAEE55 pKa = 5.05 GATVPVPLPLLTDD68 pKa = 3.6 VVDD71 pKa = 4.12 SAKK74 pKa = 10.15 EE75 pKa = 3.93 CPGEE79 pKa = 4.38 CIHH82 pKa = 6.5 VRR84 pKa = 11.84 RR85 pKa = 11.84 VLDD88 pKa = 3.4 KK89 pKa = 11.44 VEE91 pKa = 4.34 VYY93 pKa = 10.91 GPDD96 pKa = 3.36 AEE98 pKa = 4.31
Molecular weight: 10.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.541
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.935
Wikipedia 3.643
Rodwell 3.567
Grimsley 3.452
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.012
Thurlkill 3.592
EMBOSS 3.643
Sillero 3.846
Patrickios 0.846
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A510TB78|A0A510TB78_9ACTN Aldehyde dehydrogenase OS=Streptomyces sp. 1-11 OX=2590549 GN=TNCT1_04510 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.75 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7037
0
7037
2285382
39
6104
324.8
34.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.907 ± 0.054
0.796 ± 0.007
5.938 ± 0.024
5.619 ± 0.035
2.654 ± 0.019
9.738 ± 0.028
2.327 ± 0.014
2.88 ± 0.022
2.075 ± 0.025
10.322 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.676 ± 0.012
1.7 ± 0.019
6.236 ± 0.029
2.706 ± 0.018
8.407 ± 0.039
4.913 ± 0.023
6.04 ± 0.03
8.478 ± 0.028
1.504 ± 0.013
2.087 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here