Amino acid dipepetide frequency for Pseudomonas sp. BAY1663

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.051AlaAla: 14.051 ± 0.139
1.411AlaCys: 1.411 ± 0.034
5.888AlaAsp: 5.888 ± 0.076
7.888AlaGlu: 7.888 ± 0.088
3.851AlaPhe: 3.851 ± 0.054
9.893AlaGly: 9.893 ± 0.096
2.297AlaHis: 2.297 ± 0.038
5.269AlaIle: 5.269 ± 0.073
3.265AlaLys: 3.265 ± 0.061
14.243AlaLeu: 14.243 ± 0.129
3.08AlaMet: 3.08 ± 0.049
2.718AlaAsn: 2.718 ± 0.048
4.856AlaPro: 4.856 ± 0.082
5.067AlaGln: 5.067 ± 0.071
8.323AlaArg: 8.323 ± 0.088
6.325AlaSer: 6.325 ± 0.071
4.902AlaThr: 4.902 ± 0.066
7.478AlaVal: 7.478 ± 0.074
1.792AlaTrp: 1.792 ± 0.039
2.442AlaTyr: 2.442 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
1.282CysAla: 1.282 ± 0.031
0.239CysCys: 0.239 ± 0.016
0.539CysAsp: 0.539 ± 0.02
0.546CysGlu: 0.546 ± 0.02
0.309CysPhe: 0.309 ± 0.016
1.023CysGly: 1.023 ± 0.026
0.317CysHis: 0.317 ± 0.015
0.46CysIle: 0.46 ± 0.017
0.27CysLys: 0.27 ± 0.014
1.148CysLeu: 1.148 ± 0.026
0.246CysMet: 0.246 ± 0.014
0.254CysAsn: 0.254 ± 0.015
0.613CysPro: 0.613 ± 0.024
0.404CysGln: 0.404 ± 0.018
0.921CysArg: 0.921 ± 0.03
0.779CysSer: 0.779 ± 0.026
0.448CysThr: 0.448 ± 0.019
0.645CysVal: 0.645 ± 0.022
0.206CysTrp: 0.206 ± 0.012
0.252CysTyr: 0.252 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
5.892AspAla: 5.892 ± 0.07
0.551AspCys: 0.551 ± 0.02
2.99AspAsp: 2.99 ± 0.054
3.856AspGlu: 3.856 ± 0.052
1.998AspPhe: 1.998 ± 0.039
4.643AspGly: 4.643 ± 0.069
1.074AspHis: 1.074 ± 0.028
2.588AspIle: 2.588 ± 0.044
1.75AspLys: 1.75 ± 0.037
5.717AspLeu: 5.717 ± 0.074
1.207AspMet: 1.207 ± 0.032
1.438AspAsn: 1.438 ± 0.032
2.863AspPro: 2.863 ± 0.045
1.911AspGln: 1.911 ± 0.041
3.371AspArg: 3.371 ± 0.05
2.812AspSer: 2.812 ± 0.049
2.216AspThr: 2.216 ± 0.041
3.428AspVal: 3.428 ± 0.055
0.947AspTrp: 0.947 ± 0.027
1.695AspTyr: 1.695 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
7.255GluAla: 7.255 ± 0.084
0.459GluCys: 0.459 ± 0.018
2.647GluAsp: 2.647 ± 0.045
3.423GluGlu: 3.423 ± 0.063
1.812GluPhe: 1.812 ± 0.039
4.033GluGly: 4.033 ± 0.068
1.785GluHis: 1.785 ± 0.039
2.886GluIle: 2.886 ± 0.045
2.067GluLys: 2.067 ± 0.039
7.646GluLeu: 7.646 ± 0.091
1.314GluMet: 1.314 ± 0.033
1.527GluAsn: 1.527 ± 0.028
2.826GluPro: 2.826 ± 0.049
4.003GluGln: 4.003 ± 0.066
5.748GluArg: 5.748 ± 0.075
2.606GluSer: 2.606 ± 0.048
2.615GluThr: 2.615 ± 0.047
4.279GluVal: 4.279 ± 0.064
0.719GluTrp: 0.719 ± 0.022
1.256GluTyr: 1.256 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
4.117PheAla: 4.117 ± 0.064
0.455PheCys: 0.455 ± 0.017
2.387PheAsp: 2.387 ± 0.047
2.115PheGlu: 2.115 ± 0.039
1.373PhePhe: 1.373 ± 0.04
3.25PheGly: 3.25 ± 0.055
0.737PheHis: 0.737 ± 0.024
1.703PheIle: 1.703 ± 0.038
1.06PheLys: 1.06 ± 0.029
3.195PheLeu: 3.195 ± 0.048
0.767PheMet: 0.767 ± 0.023
1.111PheAsn: 1.111 ± 0.025
1.414PhePro: 1.414 ± 0.032
1.199PheGln: 1.199 ± 0.027
1.957PheArg: 1.957 ± 0.036
2.359PheSer: 2.359 ± 0.046
1.674PheThr: 1.674 ± 0.032
2.479PheVal: 2.479 ± 0.044
0.535PheTrp: 0.535 ± 0.021
0.975PheTyr: 0.975 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
7.672GlyAla: 7.672 ± 0.086
1.064GlyCys: 1.064 ± 0.029
4.081GlyAsp: 4.081 ± 0.061
5.284GlyGlu: 5.284 ± 0.07
3.271GlyPhe: 3.271 ± 0.052
6.352GlyGly: 6.352 ± 0.1
1.912GlyHis: 1.912 ± 0.036
4.211GlyIle: 4.211 ± 0.056
3.167GlyLys: 3.167 ± 0.053
9.476GlyLeu: 9.476 ± 0.116
2.377GlyMet: 2.377 ± 0.045
2.267GlyAsn: 2.267 ± 0.044
2.657GlyPro: 2.657 ± 0.051
3.591GlyGln: 3.591 ± 0.064
5.608GlyArg: 5.608 ± 0.069
4.603GlySer: 4.603 ± 0.065
3.828GlyThr: 3.828 ± 0.06
5.797GlyVal: 5.797 ± 0.066
1.418GlyTrp: 1.418 ± 0.034
2.408GlyTyr: 2.408 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.477HisAla: 2.477 ± 0.041
0.354HisCys: 0.354 ± 0.018
1.259HisAsp: 1.259 ± 0.035
1.288HisGlu: 1.288 ± 0.032
0.971HisPhe: 0.971 ± 0.03
2.177HisGly: 2.177 ± 0.045
0.615HisHis: 0.615 ± 0.025
0.995HisIle: 0.995 ± 0.029
0.599HisLys: 0.599 ± 0.022
2.55HisLeu: 2.55 ± 0.046
0.532HisMet: 0.532 ± 0.02
0.591HisAsn: 0.591 ± 0.021
1.52HisPro: 1.52 ± 0.034
0.904HisGln: 0.904 ± 0.028
1.567HisArg: 1.567 ± 0.039
1.176HisSer: 1.176 ± 0.026
0.911HisThr: 0.911 ± 0.026
1.337HisVal: 1.337 ± 0.031
0.458HisTrp: 0.458 ± 0.017
0.745HisTyr: 0.745 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.758IleAla: 5.758 ± 0.068
0.472IleCys: 0.472 ± 0.016
3.195IleAsp: 3.195 ± 0.053
3.498IleGlu: 3.498 ± 0.052
1.345IlePhe: 1.345 ± 0.038
4.514IleGly: 4.514 ± 0.068
1.022IleHis: 1.022 ± 0.027
1.824IleIle: 1.824 ± 0.042
1.433IleLys: 1.433 ± 0.027
4.104IleLeu: 4.104 ± 0.068
0.787IleMet: 0.787 ± 0.023
1.487IleAsn: 1.487 ± 0.037
2.278IlePro: 2.278 ± 0.042
1.568IleGln: 1.568 ± 0.034
3.164IleArg: 3.164 ± 0.052
2.637IleSer: 2.637 ± 0.042
2.26IleThr: 2.26 ± 0.044
3.103IleVal: 3.103 ± 0.047
0.517IleTrp: 0.517 ± 0.02
1.065IleTyr: 1.065 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.714LysAla: 3.714 ± 0.063
0.188LysCys: 0.188 ± 0.012
1.483LysAsp: 1.483 ± 0.038
1.541LysGlu: 1.541 ± 0.035
0.791LysPhe: 0.791 ± 0.024
2.319LysGly: 2.319 ± 0.049
0.711LysHis: 0.711 ± 0.022
1.404LysIle: 1.404 ± 0.035
1.187LysLys: 1.187 ± 0.039
3.422LysLeu: 3.422 ± 0.05
0.631LysMet: 0.631 ± 0.024
0.88LysAsn: 0.88 ± 0.028
1.857LysPro: 1.857 ± 0.039
1.381LysGln: 1.381 ± 0.038
2.354LysArg: 2.354 ± 0.038
1.606LysSer: 1.606 ± 0.037
1.647LysThr: 1.647 ± 0.04
2.4LysVal: 2.4 ± 0.048
0.316LysTrp: 0.316 ± 0.016
0.635LysTyr: 0.635 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.207LeuAla: 15.207 ± 0.129
1.237LeuCys: 1.237 ± 0.032
6.961LeuAsp: 6.961 ± 0.076
7.121LeuGlu: 7.121 ± 0.087
4.159LeuPhe: 4.159 ± 0.065
9.586LeuGly: 9.586 ± 0.087
2.569LeuHis: 2.569 ± 0.048
5.165LeuIle: 5.165 ± 0.073
3.82LeuLys: 3.82 ± 0.06
14.441LeuLeu: 14.441 ± 0.172
2.402LeuMet: 2.402 ± 0.044
3.207LeuAsn: 3.207 ± 0.05
6.567LeuPro: 6.567 ± 0.076
4.95LeuGln: 4.95 ± 0.077
8.22LeuArg: 8.22 ± 0.081
6.632LeuSer: 6.632 ± 0.07
5.196LeuThr: 5.196 ± 0.071
7.985LeuVal: 7.985 ± 0.089
1.386LeuTrp: 1.386 ± 0.035
2.581LeuTyr: 2.581 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.694MetAla: 2.694 ± 0.043
0.156MetCys: 0.156 ± 0.009
1.044MetAsp: 1.044 ± 0.028
0.995MetGlu: 0.995 ± 0.029
0.643MetPhe: 0.643 ± 0.023
1.622MetGly: 1.622 ± 0.041
0.546MetHis: 0.546 ± 0.019
1.069MetIle: 1.069 ± 0.026
0.862MetLys: 0.862 ± 0.029
2.86MetLeu: 2.86 ± 0.052
0.482MetMet: 0.482 ± 0.018
0.849MetAsn: 0.849 ± 0.022
1.476MetPro: 1.476 ± 0.031
1.087MetGln: 1.087 ± 0.03
1.667MetArg: 1.667 ± 0.035
1.616MetSer: 1.616 ± 0.031
1.368MetThr: 1.368 ± 0.031
1.494MetVal: 1.494 ± 0.036
0.173MetTrp: 0.173 ± 0.011
0.342MetTyr: 0.342 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.888AsnAla: 2.888 ± 0.05
0.31AsnCys: 0.31 ± 0.015
1.428AsnAsp: 1.428 ± 0.033
1.399AsnGlu: 1.399 ± 0.031
0.904AsnPhe: 0.904 ± 0.027
2.372AsnGly: 2.372 ± 0.054
0.591AsnHis: 0.591 ± 0.019
1.313AsnIle: 1.313 ± 0.037
0.77AsnLys: 0.77 ± 0.024
3.205AsnLeu: 3.205 ± 0.054
0.586AsnMet: 0.586 ± 0.019
0.787AsnAsn: 0.787 ± 0.025
1.797AsnPro: 1.797 ± 0.041
1.109AsnGln: 1.109 ± 0.03
1.988AsnArg: 1.988 ± 0.042
1.332AsnSer: 1.332 ± 0.033
1.267AsnThr: 1.267 ± 0.032
1.746AsnVal: 1.746 ± 0.038
0.418AsnTrp: 0.418 ± 0.017
0.694AsnTyr: 0.694 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
6.234ProAla: 6.234 ± 0.077
0.48ProCys: 0.48 ± 0.021
2.84ProAsp: 2.84 ± 0.041
3.494ProGlu: 3.494 ± 0.051
1.746ProPhe: 1.746 ± 0.033
4.206ProGly: 4.206 ± 0.058
1.073ProHis: 1.073 ± 0.029
1.995ProIle: 1.995 ± 0.04
1.342ProLys: 1.342 ± 0.03
5.973ProLeu: 5.973 ± 0.067
1.18ProMet: 1.18 ± 0.029
1.268ProAsn: 1.268 ± 0.027
2.165ProPro: 2.165 ± 0.048
2.277ProGln: 2.277 ± 0.037
3.224ProArg: 3.224 ± 0.055
2.749ProSer: 2.749 ± 0.046
2.206ProThr: 2.206 ± 0.036
3.626ProVal: 3.626 ± 0.061
0.796ProTrp: 0.796 ± 0.03
1.203ProTyr: 1.203 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
6.247GlnAla: 6.247 ± 0.088
0.346GlnCys: 0.346 ± 0.014
1.769GlnAsp: 1.769 ± 0.036
2.02GlnGlu: 2.02 ± 0.038
1.252GlnPhe: 1.252 ± 0.034
3.343GlnGly: 3.343 ± 0.058
1.148GlnHis: 1.148 ± 0.032
1.838GlnIle: 1.838 ± 0.041
1.082GlnLys: 1.082 ± 0.031
5.69GlnLeu: 5.69 ± 0.069
0.958GlnMet: 0.958 ± 0.029
0.929GlnAsn: 0.929 ± 0.027
2.571GlnPro: 2.571 ± 0.045
2.78GlnGln: 2.78 ± 0.061
4.12GlnArg: 4.12 ± 0.066
2.026GlnSer: 2.026 ± 0.042
1.762GlnThr: 1.762 ± 0.037
3.356GlnVal: 3.356 ± 0.05
0.627GlnTrp: 0.627 ± 0.021
0.883GlnTyr: 0.883 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
7.064ArgAla: 7.064 ± 0.073
0.872ArgCys: 0.872 ± 0.027
3.767ArgAsp: 3.767 ± 0.055
4.727ArgGlu: 4.727 ± 0.062
2.971ArgPhe: 2.971 ± 0.047
4.475ArgGly: 4.475 ± 0.055
1.997ArgHis: 1.997 ± 0.041
3.736ArgIle: 3.736 ± 0.052
2.145ArgLys: 2.145 ± 0.042
9.492ArgLeu: 9.492 ± 0.117
1.881ArgMet: 1.881 ± 0.038
2.018ArgAsn: 2.018 ± 0.039
3.47ArgPro: 3.47 ± 0.053
3.984ArgGln: 3.984 ± 0.07
5.642ArgArg: 5.642 ± 0.089
3.923ArgSer: 3.923 ± 0.053
2.97ArgThr: 2.97 ± 0.045
4.727ArgVal: 4.727 ± 0.062
1.227ArgTrp: 1.227 ± 0.031
2.178ArgTyr: 2.178 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
6.03SerAla: 6.03 ± 0.066
0.616SerCys: 0.616 ± 0.024
2.74SerAsp: 2.74 ± 0.05
3.052SerGlu: 3.052 ± 0.051
2.012SerPhe: 2.012 ± 0.037
5.195SerGly: 5.195 ± 0.069
1.229SerHis: 1.229 ± 0.025
2.651SerIle: 2.651 ± 0.046
1.514SerLys: 1.514 ± 0.038
6.582SerLeu: 6.582 ± 0.079
1.368SerMet: 1.368 ± 0.033
1.504SerAsn: 1.504 ± 0.035
2.806SerPro: 2.806 ± 0.048
2.198SerGln: 2.198 ± 0.044
4.015SerArg: 4.015 ± 0.054
3.379SerSer: 3.379 ± 0.064
2.671SerThr: 2.671 ± 0.047
3.503SerVal: 3.503 ± 0.051
0.839SerTrp: 0.839 ± 0.027
1.322SerTyr: 1.322 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
4.895ThrAla: 4.895 ± 0.067
0.533ThrCys: 0.533 ± 0.02
2.145ThrAsp: 2.145 ± 0.043
2.244ThrGlu: 2.244 ± 0.044
1.608ThrPhe: 1.608 ± 0.035
4.005ThrGly: 4.005 ± 0.06
1.0ThrHis: 1.0 ± 0.026
1.841ThrIle: 1.841 ± 0.041
0.997ThrLys: 0.997 ± 0.03
6.41ThrLeu: 6.41 ± 0.064
0.813ThrMet: 0.813 ± 0.025
1.023ThrAsn: 1.023 ± 0.03
3.017ThrPro: 3.017 ± 0.046
1.698ThrGln: 1.698 ± 0.035
3.311ThrArg: 3.311 ± 0.053
2.497ThrSer: 2.497 ± 0.042
2.252ThrThr: 2.252 ± 0.051
3.158ThrVal: 3.158 ± 0.052
0.649ThrTrp: 0.649 ± 0.027
1.09ThrTyr: 1.09 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
7.833ValAla: 7.833 ± 0.079
0.689ValCys: 0.689 ± 0.024
3.856ValAsp: 3.856 ± 0.053
4.588ValGlu: 4.588 ± 0.063
2.413ValPhe: 2.413 ± 0.046
5.095ValGly: 5.095 ± 0.075
1.423ValHis: 1.423 ± 0.034
3.56ValIle: 3.56 ± 0.056
2.045ValLys: 2.045 ± 0.048
7.888ValLeu: 7.888 ± 0.083
1.615ValMet: 1.615 ± 0.035
1.962ValAsn: 1.962 ± 0.039
3.302ValPro: 3.302 ± 0.052
2.548ValGln: 2.548 ± 0.039
4.587ValArg: 4.587 ± 0.064
3.91ValSer: 3.91 ± 0.058
3.292ValThr: 3.292 ± 0.051
5.21ValVal: 5.21 ± 0.07
0.844ValTrp: 0.844 ± 0.025
1.473ValTyr: 1.473 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.262TrpAla: 1.262 ± 0.035
0.184TrpCys: 0.184 ± 0.012
0.607TrpAsp: 0.607 ± 0.023
0.609TrpGlu: 0.609 ± 0.021
0.52TrpPhe: 0.52 ± 0.019
0.879TrpGly: 0.879 ± 0.026
0.41TrpHis: 0.41 ± 0.02
0.663TrpIle: 0.663 ± 0.021
0.415TrpLys: 0.415 ± 0.018
2.297TrpLeu: 2.297 ± 0.053
0.384TrpMet: 0.384 ± 0.017
0.428TrpAsn: 0.428 ± 0.019
0.755TrpPro: 0.755 ± 0.025
0.9TrpGln: 0.9 ± 0.029
1.279TrpArg: 1.279 ± 0.035
0.852TrpSer: 0.852 ± 0.024
0.632TrpThr: 0.632 ± 0.02
0.862TrpVal: 0.862 ± 0.024
0.252TrpTrp: 0.252 ± 0.013
0.333TrpTyr: 0.333 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.466TyrAla: 2.466 ± 0.046
0.285TyrCys: 0.285 ± 0.014
1.321TyrAsp: 1.321 ± 0.035
1.217TyrGlu: 1.217 ± 0.03
0.916TyrPhe: 0.916 ± 0.027
2.063TyrGly: 2.063 ± 0.041
0.566TyrHis: 0.566 ± 0.023
0.861TyrIle: 0.861 ± 0.025
0.674TyrLys: 0.674 ± 0.022
3.053TyrLeu: 3.053 ± 0.052
0.436TyrMet: 0.436 ± 0.02
0.655TyrAsn: 0.655 ± 0.023
1.265TyrPro: 1.265 ± 0.029
1.189TyrGln: 1.189 ± 0.032
2.201TyrArg: 2.201 ± 0.042
1.408TyrSer: 1.408 ± 0.031
1.071TyrThr: 1.071 ± 0.03
1.521TyrVal: 1.521 ± 0.031
0.404TyrTrp: 0.404 ± 0.019
0.705TyrTyr: 0.705 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5053 proteins (1391436 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski