Vibrio palustris
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3328 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R4B721|A0A1R4B721_9VIBR Uncharacterized protein OS=Vibrio palustris OX=1918946 GN=VPAL9027_02667 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 10.37 KK3 pKa = 10.17 LFPAITGLLLIGAYY17 pKa = 10.09 SLPTYY22 pKa = 9.95 AASNNDD28 pKa = 2.81 SGLYY32 pKa = 9.63 VGGNVGYY39 pKa = 10.74 VKK41 pKa = 10.0 IDD43 pKa = 3.5 GQDD46 pKa = 3.63 DD47 pKa = 3.82 FDD49 pKa = 7.01 DD50 pKa = 5.03 DD51 pKa = 4.39 NEE53 pKa = 4.52 VYY55 pKa = 10.32 QGLLGYY61 pKa = 10.25 RR62 pKa = 11.84 INPYY66 pKa = 10.41 LGVEE70 pKa = 4.43 GSYY73 pKa = 10.65 IDD75 pKa = 3.57 FGKK78 pKa = 10.92 YY79 pKa = 9.95 GSSLANAKK87 pKa = 9.0 TDD89 pKa = 3.41 GYY91 pKa = 9.2 TAALKK96 pKa = 8.95 LTAPIGDD103 pKa = 3.7 RR104 pKa = 11.84 VDD106 pKa = 5.24 LYY108 pKa = 11.65 AKK110 pKa = 10.43 GGQLWYY116 pKa = 8.93 NTDD119 pKa = 3.12 YY120 pKa = 11.33 DD121 pKa = 3.58 IAGASGDD128 pKa = 3.88 KK129 pKa = 10.85 DD130 pKa = 3.54 DD131 pKa = 4.18 TAVFAGAGVGFKK143 pKa = 9.58 VTDD146 pKa = 3.56 NFIVNAEE153 pKa = 4.15 YY154 pKa = 9.15 TWYY157 pKa = 10.35 DD158 pKa = 2.52 VDD160 pKa = 6.32 LDD162 pKa = 4.13 VKK164 pKa = 10.24 DD165 pKa = 4.04 VRR167 pKa = 11.84 DD168 pKa = 4.04 GSDD171 pKa = 2.73 TNTDD175 pKa = 4.01 FNQVTAGVEE184 pKa = 3.94 YY185 pKa = 10.77 RR186 pKa = 11.84 FF187 pKa = 3.97
Molecular weight: 20.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.923
IPC_protein 3.948
Toseland 3.706
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.948
Rodwell 3.77
Grimsley 3.605
Solomon 3.961
Lehninger 3.91
Nozaki 4.075
DTASelect 4.406
Thurlkill 3.77
EMBOSS 3.948
Sillero 4.075
Patrickios 1.163
IPC_peptide 3.948
IPC2_peptide 4.05
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|A0A1R4B7G9|A0A1R4B7G9_9VIBR Probable chromosome-partitioning protein ParB OS=Vibrio palustris OX=1918946 GN=parB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 9.34 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSKK44 pKa = 10.84
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3328
0
3328
1057203
29
2360
317.7
35.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.53 ± 0.04
1.069 ± 0.014
5.614 ± 0.035
5.853 ± 0.043
3.971 ± 0.027
6.735 ± 0.038
2.61 ± 0.025
6.385 ± 0.035
5.048 ± 0.032
10.192 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.789 ± 0.019
4.15 ± 0.025
3.949 ± 0.028
4.908 ± 0.036
4.706 ± 0.03
6.569 ± 0.029
5.561 ± 0.026
6.979 ± 0.037
1.278 ± 0.019
3.106 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here