Lachnospira eligens (strain ATCC 27750 / DSM 3376 / VPI C15-48 / C15-B4) (Eubacterium eligens)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnospira; Lachnospira eligens

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2761 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4Z6U9|C4Z6U9_LACE2 HTH marR-type domain-containing protein OS=Lachnospira eligens (strain ATCC 27750 / DSM 3376 / VPI C15-48 / C15-B4) OX=515620 GN=EUBELI_20547 PE=4 SV=1
MM1 pKa = 7.84EE2 pKa = 6.07DD3 pKa = 3.99CFMSDD8 pKa = 2.37IHH10 pKa = 8.3SVDD13 pKa = 3.66VEE15 pKa = 4.31DD16 pKa = 4.96DD17 pKa = 3.56QFAYY21 pKa = 10.26RR22 pKa = 11.84YY23 pKa = 7.69DD24 pKa = 3.67TQLLIDD30 pKa = 4.96RR31 pKa = 11.84RR32 pKa = 11.84DD33 pKa = 3.29EE34 pKa = 4.99DD35 pKa = 4.09LDD37 pKa = 3.92EE38 pKa = 5.88DD39 pKa = 4.72VIADD43 pKa = 4.09YY44 pKa = 9.21ITEE47 pKa = 4.14HH48 pKa = 7.03FEE50 pKa = 4.47GNCLIAAGDD59 pKa = 3.55EE60 pKa = 4.08DD61 pKa = 4.74LIKK64 pKa = 10.55IHH66 pKa = 6.36FHH68 pKa = 5.83TNEE71 pKa = 3.48PWKK74 pKa = 10.54VLEE77 pKa = 4.12YY78 pKa = 10.53CSTIGEE84 pKa = 4.21IYY86 pKa = 10.36DD87 pKa = 3.51IVVEE91 pKa = 4.41DD92 pKa = 5.3MIRR95 pKa = 11.84QSAGLHH101 pKa = 5.25GG102 pKa = 4.22

Molecular weight: 11.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4Z5F2|C4Z5F2_LACE2 DNA topoisomerase 1 OS=Lachnospira eligens (strain ATCC 27750 / DSM 3376 / VPI C15-48 / C15-B4) OX=515620 GN=topA PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.76VHH16 pKa = 5.91GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.79VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2761

0

2761

855239

39

1731

309.8

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.225 ± 0.05

1.509 ± 0.018

6.28 ± 0.038

6.968 ± 0.054

4.027 ± 0.035

6.794 ± 0.041

1.565 ± 0.018

8.263 ± 0.045

7.414 ± 0.038

8.058 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.228 ± 0.025

5.326 ± 0.04

2.824 ± 0.023

2.718 ± 0.024

3.88 ± 0.034

6.077 ± 0.038

5.439 ± 0.04

7.18 ± 0.038

0.773 ± 0.015

4.452 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski