Aeribacillus pallidus
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3519 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A163ZUK5|A0A163ZUK5_9BACI Acetoin utilization protein AcuB OS=Aeribacillus pallidus OX=33936 GN=AZI98_05825 PE=4 SV=1
MM1 pKa = 7.69 NEE3 pKa = 3.57 EE4 pKa = 5.76 DD5 pKa = 3.29 IQQFQNVIKK14 pKa = 10.31 IYY16 pKa = 10.52 VLSDD20 pKa = 3.18 EE21 pKa = 4.5 QLNEE25 pKa = 3.43 EE26 pKa = 4.46 DD27 pKa = 4.15 ADD29 pKa = 3.73 IFRR32 pKa = 11.84 EE33 pKa = 4.02 FAMDD37 pKa = 4.28 LVDD40 pKa = 5.89 GKK42 pKa = 10.21 DD43 pKa = 3.6 FCALILDD50 pKa = 3.91 FHH52 pKa = 7.48 VNGEE56 pKa = 4.14 LFEE59 pKa = 4.55 NLPLDD64 pKa = 4.53 LKK66 pKa = 11.39 VEE68 pKa = 4.63 DD69 pKa = 3.95 YY70 pKa = 11.27 QKK72 pKa = 10.76 ILHH75 pKa = 6.36 AVNSEE80 pKa = 3.78 YY81 pKa = 10.82 DD82 pKa = 3.1 ISYY85 pKa = 11.13 VNLDD89 pKa = 2.77 HH90 pKa = 7.49 WFYY93 pKa = 11.36 LSQDD97 pKa = 3.05
Molecular weight: 11.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.605
ProMoST 3.923
Dawson 3.808
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.139
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.935
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.845
Protein with the highest isoelectric point:
>tr|A0A165Y224|A0A165Y224_9BACI Cyclic pyranopterin monophosphate synthase OS=Aeribacillus pallidus OX=33936 GN=moaC PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.21 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 SKK14 pKa = 9.59 VHH16 pKa = 5.83 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSTKK25 pKa = 10.29 NGRR28 pKa = 11.84 KK29 pKa = 8.49 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3519
0
3519
1010804
26
2396
287.2
32.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.911 ± 0.04
0.785 ± 0.013
4.834 ± 0.03
7.821 ± 0.055
4.682 ± 0.041
6.703 ± 0.037
2.181 ± 0.017
8.264 ± 0.043
7.672 ± 0.039
9.74 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.61 ± 0.019
4.334 ± 0.026
3.626 ± 0.027
3.714 ± 0.027
4.127 ± 0.031
5.716 ± 0.029
4.92 ± 0.023
6.896 ± 0.029
0.993 ± 0.017
3.47 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here