Methylomonas lenta
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4162 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A177NEH9|A0A177NEH9_9GAMM Uncharacterized protein OS=Methylomonas lenta OX=980561 GN=A1359_07395 PE=4 SV=1
MM1 pKa = 7.27 TATTKK6 pKa = 10.69 LFEE9 pKa = 5.08 LGQLAEE15 pKa = 4.01 ASYY18 pKa = 12.0 ADD20 pKa = 3.65 FTVASVNGTINPDD33 pKa = 3.46 TMSKK37 pKa = 10.91 ALDD40 pKa = 3.62 NSSLFSLSQANEE52 pKa = 4.28 FAAHH56 pKa = 5.48 WQVASHH62 pKa = 6.71 QPDD65 pKa = 3.44 TSNGFSATLFQRR77 pKa = 11.84 IDD79 pKa = 3.75 DD80 pKa = 4.65 DD81 pKa = 5.03 PISGYY86 pKa = 10.27 ISGQYY91 pKa = 10.13 VYY93 pKa = 10.75 AIRR96 pKa = 11.84 GTMGSDD102 pKa = 3.3 DD103 pKa = 5.3 LFVADD108 pKa = 5.01 GGDD111 pKa = 2.95 IVMDD115 pKa = 4.35 GLAIDD120 pKa = 5.07 QIIDD124 pKa = 5.19 LYY126 pKa = 11.57 NDD128 pKa = 3.24 WQRR131 pKa = 11.84 INTGKK136 pKa = 10.6 DD137 pKa = 2.69 KK138 pKa = 10.62 TYY140 pKa = 10.79 QAAKK144 pKa = 10.35 LVTLTDD150 pKa = 3.46 EE151 pKa = 4.13 TALRR155 pKa = 11.84 LTMIGSPVLLVAYY168 pKa = 9.24 DD169 pKa = 3.43 ILMASKK175 pKa = 10.71 GYY177 pKa = 10.31 IIDD180 pKa = 3.97 SPSGRR185 pKa = 11.84 VRR187 pKa = 11.84 QVEE190 pKa = 4.17 FDD192 pKa = 3.36 HH193 pKa = 7.06 SDD195 pKa = 3.4 LVFGADD201 pKa = 3.69 DD202 pKa = 3.86 PRR204 pKa = 11.84 AKK206 pKa = 10.7 GDD208 pKa = 3.31 VDD210 pKa = 3.63 INSALVDD217 pKa = 3.8 VTGHH221 pKa = 5.9 SLGGHH226 pKa = 6.74 LAAAFTRR233 pKa = 11.84 LFGGNAEE240 pKa = 4.24 ALTINGAGYY249 pKa = 8.45 ATGFFPGLSLNAGGNIANLFEE270 pKa = 4.73 KK271 pKa = 10.77 LSGSAQFDD279 pKa = 3.7 PNAINNLYY287 pKa = 10.9 AEE289 pKa = 4.95 ANPEE293 pKa = 4.46 FITMDD298 pKa = 3.57 SEE300 pKa = 4.92 LGLVQQGHH308 pKa = 5.32 TDD310 pKa = 3.46 GVFVEE315 pKa = 6.02 QSQPWQQTFGHH326 pKa = 6.79 GSGQMTDD333 pKa = 2.95 SLAVYY338 pKa = 10.4 DD339 pKa = 4.52 LFIRR343 pKa = 11.84 LDD345 pKa = 3.55 SGLLVATPGPVLEE358 pKa = 4.2 KK359 pKa = 10.69 LKK361 pKa = 10.54 PLFEE365 pKa = 4.64 AASNQASSSLEE376 pKa = 3.77 NLVNALVDD384 pKa = 4.95 LYY386 pKa = 11.07 DD387 pKa = 3.64 TGIKK391 pKa = 9.91 IADD394 pKa = 3.89 LATDD398 pKa = 3.45 NRR400 pKa = 11.84 EE401 pKa = 4.12 ALYY404 pKa = 10.77 QAMNVIKK411 pKa = 10.66 DD412 pKa = 3.37 SDD414 pKa = 4.22 SYY416 pKa = 11.67 QSANGLTDD424 pKa = 3.24 VVLINDD430 pKa = 4.21 VGASQCVEE438 pKa = 3.87 KK439 pKa = 10.89 AKK441 pKa = 9.27 QTDD444 pKa = 3.33 AEE446 pKa = 4.9 GIAFRR451 pKa = 11.84 YY452 pKa = 9.67 ALLHH456 pKa = 6.12 LNPFVLVDD464 pKa = 3.7 ADD466 pKa = 3.79 YY467 pKa = 11.49 SRR469 pKa = 11.84 HH470 pKa = 5.62 NGNGEE475 pKa = 4.16 LDD477 pKa = 4.09 LYY479 pKa = 10.48 DD480 pKa = 4.25 AKK482 pKa = 10.24 TGHH485 pKa = 5.93 GQITEE490 pKa = 3.55 QWLNDD495 pKa = 3.59 RR496 pKa = 11.84 AQMLSHH502 pKa = 7.36 LLQRR506 pKa = 11.84 NLDD509 pKa = 3.91 DD510 pKa = 5.12 ASFASAIFADD520 pKa = 5.24 QIFEE524 pKa = 5.19 DD525 pKa = 4.21 YY526 pKa = 9.7 TLPGVIDD533 pKa = 3.91 GTHH536 pKa = 7.32 LILTGPEE543 pKa = 3.89 TLGEE547 pKa = 4.1 NSHH550 pKa = 6.88 HH551 pKa = 7.0 IIFGDD556 pKa = 3.61 DD557 pKa = 3.41 QANQLQGGNLGDD569 pKa = 4.0 HH570 pKa = 7.31 LYY572 pKa = 10.78 GASGDD577 pKa = 4.04 DD578 pKa = 4.17 LLTGGKK584 pKa = 10.43 GNDD587 pKa = 3.49 YY588 pKa = 10.53 MEE590 pKa = 5.26 GGSGSDD596 pKa = 3.33 LYY598 pKa = 11.66 AFIAGDD604 pKa = 3.8 GMDD607 pKa = 4.96 TILDD611 pKa = 3.63 TDD613 pKa = 4.01 GLGKK617 pKa = 8.69 ITLNGVQIKK626 pKa = 10.09 GQTDD630 pKa = 3.28 VAANQWIQAGQTWQDD645 pKa = 3.33 RR646 pKa = 11.84 QHH648 pKa = 6.77 HH649 pKa = 6.06 IGYY652 pKa = 9.54 NLVTQADD659 pKa = 4.28 GTQDD663 pKa = 4.09 LLIATADD670 pKa = 3.21 NTLIVKK676 pKa = 9.32 NWHH679 pKa = 6.54 ADD681 pKa = 3.35 VLGITLEE688 pKa = 4.43 SVTPPEE694 pKa = 4.06 PEE696 pKa = 4.66 IILTVTGDD704 pKa = 4.56 FKK706 pKa = 11.36 PLDD709 pKa = 3.78 QDD711 pKa = 4.05 PNTDD715 pKa = 4.62 GIQLGYY721 pKa = 10.51 DD722 pKa = 3.02 ALGNVITDD730 pKa = 4.23 PEE732 pKa = 4.39 QPDD735 pKa = 3.93 PDD737 pKa = 5.13 HH738 pKa = 7.27 EE739 pKa = 5.22 DD740 pKa = 3.21 SLYY743 pKa = 11.23 DD744 pKa = 3.47 STDD747 pKa = 3.12 NDD749 pKa = 3.48 KK750 pKa = 11.43 LQGFGGDD757 pKa = 4.84 DD758 pKa = 3.14 ILDD761 pKa = 4.98 AIRR764 pKa = 11.84 GGDD767 pKa = 3.63 DD768 pKa = 3.67 LLEE771 pKa = 4.83 GGAGSDD777 pKa = 3.62 VLNGGSGNDD786 pKa = 3.38 FLYY789 pKa = 10.98 ADD791 pKa = 4.16 VEE793 pKa = 4.28 ISVADD798 pKa = 4.17 ALAQADD804 pKa = 4.1 TQAATDD810 pKa = 3.67 QRR812 pKa = 11.84 GDD814 pKa = 3.12 WLYY817 pKa = 11.53 GGDD820 pKa = 5.72 DD821 pKa = 4.99 DD822 pKa = 6.07 DD823 pKa = 4.71 TLLGDD828 pKa = 4.81 GGNDD832 pKa = 3.18 MLLGGSGADD841 pKa = 3.67 LLIGGGGDD849 pKa = 4.02 DD850 pKa = 4.87 NLFGDD855 pKa = 3.84 KK856 pKa = 10.01 TGNANLDD863 pKa = 3.06 WDD865 pKa = 4.28 VFRR868 pKa = 11.84 STINSNNDD876 pKa = 2.55 STYY879 pKa = 8.0 TLTYY883 pKa = 9.98 FRR885 pKa = 11.84 STAYY889 pKa = 10.33 DD890 pKa = 3.57 NPVGGDD896 pKa = 3.61 DD897 pKa = 3.47 ILYY900 pKa = 10.62 GGEE903 pKa = 4.14 GNDD906 pKa = 3.22 WLIGNRR912 pKa = 11.84 GNDD915 pKa = 3.25 ILDD918 pKa = 3.74 GGVGNDD924 pKa = 3.35 VLFGGEE930 pKa = 4.8 DD931 pKa = 3.48 KK932 pKa = 11.38 DD933 pKa = 4.2 FLLGGDD939 pKa = 4.65 GNDD942 pKa = 3.37 EE943 pKa = 4.47 LYY945 pKa = 11.37 GNAGVSSPLEE955 pKa = 4.69 DD956 pKa = 4.49 GDD958 pKa = 5.42 DD959 pKa = 3.84 YY960 pKa = 11.98 LDD962 pKa = 4.46 GGTGNDD968 pKa = 3.54 KK969 pKa = 11.06 LVGGGGNDD977 pKa = 3.26 NLFGGLGDD985 pKa = 3.92 DD986 pKa = 4.85 VLYY989 pKa = 10.98 GDD991 pKa = 4.99 HH992 pKa = 7.09 NGTLEE997 pKa = 4.04 ADD999 pKa = 3.3 EE1000 pKa = 5.32 GEE1002 pKa = 4.75 DD1003 pKa = 3.7 YY1004 pKa = 11.33 LVGGDD1009 pKa = 4.93 GSDD1012 pKa = 3.37 TLIGGGKK1019 pKa = 9.77 NDD1021 pKa = 3.86 ILLGGAGIDD1030 pKa = 3.75 YY1031 pKa = 10.81 LFGEE1035 pKa = 4.87 AGDD1038 pKa = 5.59 DD1039 pKa = 3.58 IMQGGADD1046 pKa = 3.83 ADD1048 pKa = 4.19 EE1049 pKa = 4.85 LSGGAGMDD1057 pKa = 3.76 YY1058 pKa = 11.18 LDD1060 pKa = 5.15 GGDD1063 pKa = 4.2 GNDD1066 pKa = 4.31 IIDD1069 pKa = 4.11 GGEE1072 pKa = 4.19 GNDD1075 pKa = 3.89 TLLGGAGADD1084 pKa = 3.44 ILQGGADD1091 pKa = 4.05 DD1092 pKa = 6.06 DD1093 pKa = 4.95 VYY1095 pKa = 11.81 LDD1097 pKa = 3.49 VTAEE1101 pKa = 3.97 DD1102 pKa = 4.58 TINDD1106 pKa = 3.73 ILGHH1110 pKa = 5.69 SIIHH1114 pKa = 6.66 LSQASGLAADD1124 pKa = 4.13 NALSKK1129 pKa = 10.68 VDD1131 pKa = 3.61 QNGVDD1136 pKa = 4.33 LAITLDD1142 pKa = 3.47 NGEE1145 pKa = 4.89 TITLSNALYY1154 pKa = 9.87 MDD1156 pKa = 4.05 ATLVFAGGSEE1166 pKa = 4.07 MDD1168 pKa = 4.23 LEE1170 pKa = 4.47 TLVGNQLTGAVSLQLGNNGGKK1191 pKa = 10.53 LYY1193 pKa = 10.84 GGAGADD1199 pKa = 3.47 SLYY1202 pKa = 11.0 GGSGNDD1208 pKa = 3.72 TLNGALGADD1217 pKa = 3.63 KK1218 pKa = 10.95 LYY1220 pKa = 10.94 GYY1222 pKa = 10.49 AGNDD1226 pKa = 3.34 VLNGGDD1232 pKa = 4.42 GDD1234 pKa = 5.28 DD1235 pKa = 4.53 ILDD1238 pKa = 4.57 GGDD1241 pKa = 4.3 GDD1243 pKa = 4.16 DD1244 pKa = 4.16 TLIGGAGRR1252 pKa = 11.84 DD1253 pKa = 3.71 LLLGGSGDD1261 pKa = 4.55 DD1262 pKa = 3.86 SYY1264 pKa = 12.08 QLTSVAGGDD1273 pKa = 4.67 KK1274 pKa = 10.64 IADD1277 pKa = 3.7 TQGQNLIKK1285 pKa = 10.2 FGSDD1289 pKa = 2.56 IDD1291 pKa = 4.33 PNQLTASVSTLAGQTALTLTLAGVEE1316 pKa = 4.45 LATITQGYY1324 pKa = 9.4 SSFGFEE1330 pKa = 4.26 FADD1333 pKa = 4.11 GTRR1336 pKa = 11.84 LTTDD1340 pKa = 4.05 DD1341 pKa = 4.34 FLLSYY1346 pKa = 9.59 RR1347 pKa = 11.84 TGSGTGNIVGTNNDD1361 pKa = 3.26 DD1362 pKa = 3.85 TLFGGQTADD1371 pKa = 3.64 KK1372 pKa = 10.43 LYY1374 pKa = 10.84 GYY1376 pKa = 10.32 AGNDD1380 pKa = 3.72 TLWGGRR1386 pKa = 11.84 GDD1388 pKa = 4.8 DD1389 pKa = 3.99 EE1390 pKa = 5.79 LEE1392 pKa = 4.63 GGLGSDD1398 pKa = 4.21 DD1399 pKa = 3.18 YY1400 pKa = 11.22 RR1401 pKa = 11.84 YY1402 pKa = 10.55 RR1403 pKa = 11.84 LGDD1406 pKa = 3.31 GHH1408 pKa = 6.37 DD1409 pKa = 4.3 TIVEE1413 pKa = 3.98 TDD1415 pKa = 3.56 DD1416 pKa = 5.29 ANLSGQDD1423 pKa = 2.79 RR1424 pKa = 11.84 VSFGVGITSSDD1435 pKa = 3.32 VLFNRR1440 pKa = 11.84 RR1441 pKa = 11.84 PNGDD1445 pKa = 3.34 LSVTVAGLADD1455 pKa = 5.85 AITITGWYY1463 pKa = 8.77 NDD1465 pKa = 3.04 AATRR1469 pKa = 11.84 VDD1471 pKa = 3.9 SFAFADD1477 pKa = 4.4 GEE1479 pKa = 4.7 TITADD1484 pKa = 3.57 TLLGLAIAPQTGSAGNDD1501 pKa = 3.16 TLTGSGYY1508 pKa = 10.31 RR1509 pKa = 11.84 DD1510 pKa = 3.52 IIMAGAGDD1518 pKa = 5.1 DD1519 pKa = 4.29 LLTGNGGDD1527 pKa = 3.61 DD1528 pKa = 3.7 DD1529 pKa = 6.13 LYY1531 pKa = 11.69 GEE1533 pKa = 4.88 VGTDD1537 pKa = 2.97 TYY1539 pKa = 11.4 RR1540 pKa = 11.84 FTLGSGADD1548 pKa = 4.27 RR1549 pKa = 11.84 IFEE1552 pKa = 4.17 MADD1555 pKa = 3.05 EE1556 pKa = 4.22 SSIIQVSGFDD1566 pKa = 4.03 LSRR1569 pKa = 11.84 LTGNRR1574 pKa = 11.84 IGDD1577 pKa = 3.96 DD1578 pKa = 3.51 LLLGVTGAADD1588 pKa = 3.89 SLTLKK1593 pKa = 10.41 NFYY1596 pKa = 11.1 SMPHH1600 pKa = 6.49 DD1601 pKa = 3.33 WQVSVNGGGSRR1612 pKa = 11.84 DD1613 pKa = 3.73 LVGVLADD1620 pKa = 3.6 NEE1622 pKa = 4.37 AYY1624 pKa = 9.86 RR1625 pKa = 11.84 ASRR1628 pKa = 11.84 SEE1630 pKa = 4.1 LEE1632 pKa = 4.01 RR1633 pKa = 11.84 LQDD1636 pKa = 3.51 AFQSQVTDD1644 pKa = 3.5 EE1645 pKa = 4.45 VYY1647 pKa = 10.46 RR1648 pKa = 11.84 GYY1650 pKa = 10.39 QAAGMLQQADD1660 pKa = 4.44 GSWMSDD1666 pKa = 3.15 LQINLSKK1673 pKa = 10.01 KK1674 pKa = 7.85 TSDD1677 pKa = 3.26 YY1678 pKa = 10.83 TRR1680 pKa = 11.84 ADD1682 pKa = 3.34 VYY1684 pKa = 10.55 TGYY1687 pKa = 11.01 VPTNTSYY1694 pKa = 11.19 YY1695 pKa = 9.66 SLSGGYY1701 pKa = 8.67 GLKK1704 pKa = 10.25 QGGVSVSSYY1713 pKa = 10.85 TSDD1716 pKa = 3.74 DD1717 pKa = 3.42 ANINYY1722 pKa = 7.61 TYY1724 pKa = 10.51 SGSGYY1729 pKa = 10.16 LPKK1732 pKa = 10.39 NVQVTWHH1739 pKa = 6.47 EE1740 pKa = 4.07 LQAVSTQLYY1749 pKa = 8.16 TSTAVNYY1756 pKa = 8.16 SQKK1759 pKa = 10.76 LYY1761 pKa = 10.39 TIEE1764 pKa = 4.17 EE1765 pKa = 4.36 LLALIAASGGSSIAIEE1781 pKa = 4.09 NPYY1784 pKa = 10.52 VITEE1788 pKa = 4.17 TPTYY1792 pKa = 10.98 NLTTTTTTTFTATPVSIIEE1811 pKa = 4.21 ADD1813 pKa = 3.36 GTGYY1817 pKa = 10.82 DD1818 pKa = 3.29 IAGSNAQSMYY1828 pKa = 10.95 NLPNLPDD1835 pKa = 3.5 TLAINAYY1842 pKa = 9.13 DD1843 pKa = 3.67 YY1844 pKa = 10.77 RR1845 pKa = 11.84 VEE1847 pKa = 3.74 ISIVNGGNSDD1857 pKa = 3.06 NDD1859 pKa = 2.89 IRR1861 pKa = 11.84 FNGNIGIVHH1870 pKa = 6.84 GGGGNDD1876 pKa = 3.77 TIRR1879 pKa = 11.84 AYY1881 pKa = 9.84 STGIYY1886 pKa = 9.22 IDD1888 pKa = 3.67 YY1889 pKa = 10.53 QGDD1892 pKa = 3.67 VAGQTWLDD1900 pKa = 3.57 GGQGDD1905 pKa = 4.77 DD1906 pKa = 4.68 LIIGGFSHH1914 pKa = 7.23 NDD1916 pKa = 3.58 DD1917 pKa = 3.3 IVGGRR1922 pKa = 11.84 GNDD1925 pKa = 3.43 MLRR1928 pKa = 11.84 GEE1930 pKa = 4.96 GGTDD1934 pKa = 2.26 RR1935 pKa = 11.84 YY1936 pKa = 11.09 YY1937 pKa = 11.3 FLAGDD1942 pKa = 3.7 SGTDD1946 pKa = 3.22 LVYY1949 pKa = 10.86 DD1950 pKa = 3.74 VGYY1953 pKa = 10.79 GGSLYY1958 pKa = 10.7 DD1959 pKa = 3.48 DD1960 pKa = 4.78 TIAFGEE1966 pKa = 4.96 GIALSDD1972 pKa = 5.09 LSFSWGDD1979 pKa = 3.71 EE1980 pKa = 3.57 ILPSYY1985 pKa = 11.14 NYY1987 pKa = 11.0 GNYY1990 pKa = 9.83 DD1991 pKa = 3.56 EE1992 pKa = 5.0 SWVRR1996 pKa = 11.84 RR1997 pKa = 11.84 FQTLDD2002 pKa = 3.18 IRR2004 pKa = 11.84 WQPDD2008 pKa = 2.63 AVARR2012 pKa = 11.84 VVMSTPFINSYY2023 pKa = 9.28 GQIVEE2028 pKa = 4.46 SNGWDD2033 pKa = 3.3 QTGVEE2038 pKa = 4.32 VLEE2041 pKa = 4.53 FADD2044 pKa = 4.48 GSRR2047 pKa = 11.84 MSIEE2051 pKa = 3.95 QLLALPGMPVRR2062 pKa = 11.84 PTVNRR2067 pKa = 11.84 FSNIQLGTTDD2077 pKa = 4.46 ADD2079 pKa = 3.9 VLTGQANPDD2088 pKa = 3.81 DD2089 pKa = 5.05 LYY2091 pKa = 11.66 GGLGDD2096 pKa = 4.8 DD2097 pKa = 4.61 TLTGDD2102 pKa = 5.96 DD2103 pKa = 3.95 GDD2105 pKa = 4.0 DD2106 pKa = 3.46 WLFGGAGNDD2115 pKa = 3.64 LLAGGSGNDD2124 pKa = 2.58 RR2125 pKa = 11.84 YY2126 pKa = 11.13 YY2127 pKa = 11.18 FEE2129 pKa = 5.29 TEE2131 pKa = 4.19 DD2132 pKa = 3.79 SGTDD2136 pKa = 3.39 LLYY2139 pKa = 10.74 DD2140 pKa = 3.61 IGYY2143 pKa = 10.48 GGGDD2147 pKa = 3.23 VSGDD2151 pKa = 3.5 SVVFGDD2157 pKa = 5.96 DD2158 pKa = 3.65 INLSDD2163 pKa = 6.07 FNFSWGSEE2171 pKa = 3.95 NFSPFNDD2178 pKa = 3.47 GNVTLFQTLNIGWQTNSVIKK2198 pKa = 10.3 IVMPRR2203 pKa = 11.84 PDD2205 pKa = 3.31 AYY2207 pKa = 11.02 SWFNTGVEE2215 pKa = 4.44 FFEE2218 pKa = 4.46 FAYY2221 pKa = 10.07 GSQMTMDD2228 pKa = 4.59 QILALAGPSPQNAPILNSPLVDD2250 pKa = 3.31 QQVFADD2256 pKa = 3.67 VAFSYY2261 pKa = 8.34 TLPEE2265 pKa = 4.43 DD2266 pKa = 3.74 AFIDD2270 pKa = 4.07 LDD2272 pKa = 4.67 AGDD2275 pKa = 3.86 VLTYY2279 pKa = 10.12 EE2280 pKa = 5.01 SSSTWRR2286 pKa = 11.84 DD2287 pKa = 2.87 WLSFDD2292 pKa = 3.92 PEE2294 pKa = 4.24 TRR2296 pKa = 11.84 TFTGLPGADD2305 pKa = 3.01 MLGSYY2310 pKa = 10.57 NIFVTATDD2318 pKa = 3.33 RR2319 pKa = 11.84 FGASVGDD2326 pKa = 4.22 SFCLTVNPALVGTAGDD2342 pKa = 4.23 DD2343 pKa = 3.58 VLQGTEE2349 pKa = 4.39 GDD2351 pKa = 3.84 DD2352 pKa = 3.88 GMVGGAGNDD2361 pKa = 3.72 LLEE2364 pKa = 5.04 GGAGQDD2370 pKa = 3.23 TYY2372 pKa = 11.07 IINLNDD2378 pKa = 3.36 GRR2380 pKa = 11.84 DD2381 pKa = 3.86 SIVDD2385 pKa = 3.38 AVGDD2389 pKa = 3.69 NNAIRR2394 pKa = 11.84 FGPGITPNDD2403 pKa = 2.88 ITLRR2407 pKa = 11.84 LGSLMLDD2414 pKa = 3.6 LGNGNEE2420 pKa = 3.92 VHH2422 pKa = 7.06 IEE2424 pKa = 3.99 GFNPDD2429 pKa = 3.73 DD2430 pKa = 3.78 VFNSSSIDD2438 pKa = 3.15 TFTFSDD2444 pKa = 3.66 GRR2446 pKa = 11.84 VLSLEE2451 pKa = 3.87 QLLARR2456 pKa = 11.84 GFDD2459 pKa = 4.05 LDD2461 pKa = 3.55 GTAANDD2467 pKa = 3.7 VITGTNTSDD2476 pKa = 4.64 RR2477 pKa = 11.84 INGLDD2482 pKa = 4.51 GNDD2485 pKa = 3.68 TLIGGAGDD2493 pKa = 3.66 DD2494 pKa = 4.23 TYY2496 pKa = 11.86 VLSDD2500 pKa = 3.65 GGEE2503 pKa = 4.12 GMLKK2507 pKa = 9.21 IDD2509 pKa = 4.01 YY2510 pKa = 10.42 VAGTNSSVSRR2520 pKa = 11.84 YY2521 pKa = 10.06 LGTFQAGEE2529 pKa = 4.29 TVTFSARR2536 pKa = 11.84 YY2537 pKa = 7.82 QSEE2540 pKa = 4.12 PGVTARR2546 pKa = 11.84 LFLGDD2551 pKa = 4.01 AGGADD2556 pKa = 3.44 KK2557 pKa = 11.24 YY2558 pKa = 11.73 DD2559 pKa = 3.48 NYY2561 pKa = 11.01 KK2562 pKa = 8.08 YY2563 pKa = 9.51 TSVIDD2568 pKa = 3.99 GTGEE2572 pKa = 3.86 WEE2574 pKa = 4.17 TLSVSVTLTHH2584 pKa = 7.95 DD2585 pKa = 3.94 DD2586 pKa = 3.68 QLWAYY2591 pKa = 9.42 MYY2593 pKa = 11.02 GDD2595 pKa = 3.09 RR2596 pKa = 11.84 DD2597 pKa = 3.77 GPNRR2601 pKa = 11.84 QAGNFVLYY2609 pKa = 10.46 DD2610 pKa = 3.48 DD2611 pKa = 4.81 VEE2613 pKa = 4.37 IVSQQRR2619 pKa = 11.84 GVILQDD2625 pKa = 3.48 SFATGINSNWRR2636 pKa = 11.84 TSGSSQWIEE2645 pKa = 3.63 QSTDD2649 pKa = 3.34 QVVDD2653 pKa = 3.2 TSGTDD2658 pKa = 3.52 TVQSNYY2664 pKa = 10.43 SYY2666 pKa = 10.42 TLGADD2671 pKa = 3.42 IEE2673 pKa = 4.2 NLLLKK2678 pKa = 10.77 GIANIDD2684 pKa = 3.53 GTGNALDD2691 pKa = 4.04 NVLTGNSGNNILMGGEE2707 pKa = 4.3 GNDD2710 pKa = 3.74 TLLGGAGNDD2719 pKa = 3.58 RR2720 pKa = 11.84 LNGGNGDD2727 pKa = 4.96 DD2728 pKa = 4.72 ILDD2731 pKa = 4.24 GGDD2734 pKa = 3.72 GNDD2737 pKa = 3.34 TYY2739 pKa = 11.86 VLNDD2743 pKa = 3.66 GGEE2746 pKa = 4.23 GALKK2750 pKa = 9.5 IDD2752 pKa = 3.99 YY2753 pKa = 7.73 MAGTNSSVASNLGTFQQGDD2772 pKa = 3.99 TVTFSARR2779 pKa = 11.84 YY2780 pKa = 7.82 QSEE2783 pKa = 4.12 PGVTARR2789 pKa = 11.84 LFIGDD2794 pKa = 3.7 AGGADD2799 pKa = 3.38 KK2800 pKa = 11.24 YY2801 pKa = 11.75 DD2802 pKa = 3.41 NYY2804 pKa = 11.27 KK2805 pKa = 8.95 YY2806 pKa = 7.56 TTVTDD2811 pKa = 4.17 GTGEE2815 pKa = 4.07 WEE2817 pKa = 4.22 TLSVSVTLKK2826 pKa = 10.68 HH2827 pKa = 7.52 DD2828 pKa = 4.1 DD2829 pKa = 3.66 QLWAYY2834 pKa = 9.42 MYY2836 pKa = 11.02 GDD2838 pKa = 3.28 RR2839 pKa = 11.84 DD2840 pKa = 4.14 GPNHH2844 pKa = 5.65 QAGNYY2849 pKa = 8.4 VLYY2852 pKa = 10.59 DD2853 pKa = 3.46 DD2854 pKa = 4.53 VQIVSQQRR2862 pKa = 11.84 GIILQDD2868 pKa = 3.46 SFSAGIDD2875 pKa = 3.99 STWKK2879 pKa = 9.61 TSGSSHH2885 pKa = 7.22 WIEE2888 pKa = 3.84 QSTITDD2894 pKa = 3.49 QVIDD2898 pKa = 3.5 TGGIDD2903 pKa = 3.89 TVQSIYY2909 pKa = 11.03 SYY2911 pKa = 11.1 ILGDD2915 pKa = 3.86 GIEE2918 pKa = 3.96 NLEE2921 pKa = 4.41 LKK2923 pKa = 9.23 GTSDD2927 pKa = 4.04 INGTGNTLEE2936 pKa = 4.54 NSLAGNKK2943 pKa = 9.5 GNNILMGGEE2952 pKa = 4.37 GNDD2955 pKa = 3.67 TLNGGGGNDD2964 pKa = 2.86 IYY2966 pKa = 11.39 LFGKK2970 pKa = 10.58 GDD2972 pKa = 3.67 GADD2975 pKa = 3.2 ILSDD2979 pKa = 4.27 FDD2981 pKa = 3.95 TTDD2984 pKa = 2.87 GNTDD2988 pKa = 2.8 ILAFGAGIIEE2998 pKa = 3.92 EE2999 pKa = 4.51 QIWFRR3004 pKa = 11.84 HH3005 pKa = 4.98 VGSTLEE3011 pKa = 4.13 LSLIGTDD3018 pKa = 4.93 DD3019 pKa = 3.98 KK3020 pKa = 11.56 VTINNWYY3027 pKa = 9.51 SCSTYY3032 pKa = 10.27 HH3033 pKa = 6.84 VEE3035 pKa = 5.49 QFTTSDD3041 pKa = 3.4 GLTLLDD3047 pKa = 3.75 SQVEE3051 pKa = 4.15 NLVIAMAAFAPPSAGQISLPQDD3073 pKa = 3.69 YY3074 pKa = 7.83 QTTLAPVLAANWQQ3087 pKa = 3.39
Molecular weight: 326.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.617
IPC_protein 3.694
Toseland 3.439
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.35
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.126
Thurlkill 3.503
EMBOSS 3.668
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A177N6M1|A0A177N6M1_9GAMM Oxaloacetate decarboxylase beta chain OS=Methylomonas lenta OX=980561 GN=A1359_11890 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.06 LKK11 pKa = 10.38 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.12 GRR39 pKa = 11.84 ASLTVV44 pKa = 3.04
Molecular weight: 5.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.618
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.34
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4162
0
4162
1337950
44
3453
321.5
35.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.196 ± 0.04
1.062 ± 0.014
5.613 ± 0.027
5.766 ± 0.034
4.147 ± 0.026
6.683 ± 0.048
2.298 ± 0.02
6.429 ± 0.034
4.997 ± 0.035
10.982 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.018
4.206 ± 0.025
4.237 ± 0.024
4.943 ± 0.038
4.901 ± 0.03
6.235 ± 0.029
5.122 ± 0.03
6.497 ± 0.034
1.312 ± 0.014
2.929 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here