Altericroceibacterium endophyticum
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3079 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I4T3V5|A0A6I4T3V5_9SPHN Type I secretion system permease/ATPase OS=Altericroceibacterium endophyticum OX=1808508 GN=GRI91_09325 PE=4 SV=1
MM1 pKa = 7.36 FMATANIAHH10 pKa = 7.45 AGTCTEE16 pKa = 4.19 TAPGSGDD23 pKa = 3.4 WTCSGAANSGSDD35 pKa = 3.47 TPEE38 pKa = 3.67 TLGNGTGSEE47 pKa = 4.64 LIVTTASGFGIDD59 pKa = 4.09 NASGTAFILTNTAGGNNLTFTDD81 pKa = 5.21 AYY83 pKa = 10.61 ASDD86 pKa = 3.45 ISGTGNGIFAVNNGTGITSITTTGVVTGTGTGTYY120 pKa = 10.27 GIYY123 pKa = 10.56 ARR125 pKa = 11.84 NLGTDD130 pKa = 3.85 LTISAADD137 pKa = 3.35 VSGADD142 pKa = 4.22 GISVANLGTGSTSVTATGTVTGTIYY167 pKa = 10.62 RR168 pKa = 11.84 GIVVNNVSTSTNMTVSAVDD187 pKa = 3.24 VSGGTDD193 pKa = 4.28 GIYY196 pKa = 10.75 AFNNGTGFNSVTATGTVTGSAGSGILAVNYY226 pKa = 7.54 GTNLTVSAVDD236 pKa = 3.49 ASGSNSGIDD245 pKa = 3.32 ALNYY249 pKa = 7.69 GTGSNSVTATGTVTGNTNYY268 pKa = 10.78 GINARR273 pKa = 11.84 NFANSSTDD281 pKa = 3.36 LTVFAADD288 pKa = 3.17 ASGPNVGIFAYY299 pKa = 10.91 NNGTGSTDD307 pKa = 3.05 VTATGTVTSTINSGINASNSSNATNLTVSAVDD339 pKa = 3.3 VSGGTDD345 pKa = 4.44 GIRR348 pKa = 11.84 AINNGSGSNSITATGTVTGTINYY371 pKa = 7.95 GINVFGNSTATNLTVSAANASGGTNGIVTYY401 pKa = 10.98 NSGTGSTRR409 pKa = 11.84 VTATGTVAGTTSYY422 pKa = 11.03 GISAWNDD429 pKa = 2.96 STATDD434 pKa = 4.07 LTVSAADD441 pKa = 3.34 VSGGYY446 pKa = 9.63 IGIEE450 pKa = 3.97 AYY452 pKa = 11.0 NNGTGTTSVTTTGAVSAVNAGILVYY477 pKa = 10.63 SQNDD481 pKa = 3.61 TFAVSVDD488 pKa = 3.74 SGSVAAVGGGNDD500 pKa = 3.1 AYY502 pKa = 10.83 ARR504 pKa = 11.84 AIQTSGAKK512 pKa = 10.27 GGTIDD517 pKa = 3.27 IAAGAVVDD525 pKa = 5.55 GSASGIAIRR534 pKa = 11.84 DD535 pKa = 3.15 GDD537 pKa = 4.18 YY538 pKa = 10.68 ATGLGDD544 pKa = 4.08 VLDD547 pKa = 4.56 GTDD550 pKa = 3.49 LTGGDD555 pKa = 4.0 VVVTTYY561 pKa = 11.78 GDD563 pKa = 3.56 VKK565 pKa = 11.08 GDD567 pKa = 4.13 AILGKK572 pKa = 11.07 GNDD575 pKa = 3.75 TFNLAGGDD583 pKa = 3.92 FDD585 pKa = 5.64 GDD587 pKa = 3.43 IYY589 pKa = 11.36 GDD591 pKa = 3.64 DD592 pKa = 3.66 TLASVNDD599 pKa = 4.08 GNDD602 pKa = 3.07 TFNFTGGDD610 pKa = 3.03 WHH612 pKa = 8.18 SGFFGGNGSDD622 pKa = 3.47 TANISSPTYY631 pKa = 10.54 DD632 pKa = 3.13 GTAHH636 pKa = 6.31 VLDD639 pKa = 5.13 GGDD642 pKa = 4.49 DD643 pKa = 3.75 YY644 pKa = 11.75 GTGDD648 pKa = 3.25 GWIDD652 pKa = 3.42 TLTFAGVTSDD662 pKa = 4.67 ANGTNILNWEE672 pKa = 4.32 NIVINGGAITIVDD685 pKa = 3.94 GALEE689 pKa = 4.31 TGSDD693 pKa = 3.44 AGTGLTVMNGGVLDD707 pKa = 4.56 GSDD710 pKa = 3.52 ALALTGNLVIKK721 pKa = 10.71 SGGAFVGTGGGAGVYY736 pKa = 10.02 SISGDD741 pKa = 3.29 VSNQGTITTQDD752 pKa = 3.19 GVVGDD757 pKa = 3.98 VVSIGGNYY765 pKa = 9.87 SGGGTITVDD774 pKa = 3.67 ANLDD778 pKa = 3.68 GAGSADD784 pKa = 3.51 QIVIAGNVTGPATTVNLNNVGSGIISPTTGNGIFVVSAGGTSSAGSFVLNPSDD837 pKa = 3.7 AQVGAFDD844 pKa = 3.92 YY845 pKa = 11.08 SINQASDD852 pKa = 2.87 GKK854 pKa = 10.8 LYY856 pKa = 10.97 LSSTYY861 pKa = 9.82 QAGVPVMEE869 pKa = 5.86 AYY871 pKa = 9.94 GQALLGLNSLNSLRR885 pKa = 11.84 QRR887 pKa = 11.84 TGNRR891 pKa = 11.84 SWTPGLSKK899 pKa = 9.91 TIDD902 pKa = 3.74 DD903 pKa = 4.02 TSTAAGVWGQFEE915 pKa = 4.41 GSLAEE920 pKa = 4.12 QNFATSVTGSAYY932 pKa = 10.5 DD933 pKa = 3.32 QDD935 pKa = 3.81 FWRR938 pKa = 11.84 LRR940 pKa = 11.84 TGVEE944 pKa = 4.02 APLTLSDD951 pKa = 3.9 SGALFVGTQVNYY963 pKa = 10.29 GVARR967 pKa = 11.84 TNVTSVYY974 pKa = 10.06 GNGRR978 pKa = 11.84 IMTNVLGFGLGMTWYY993 pKa = 10.07 GNSGFYY999 pKa = 10.95 ADD1001 pKa = 3.05 IQGRR1005 pKa = 11.84 YY1006 pKa = 7.02 NHH1008 pKa = 6.3 YY1009 pKa = 10.86 SSDD1012 pKa = 3.22 VRR1014 pKa = 11.84 NGLGRR1019 pKa = 11.84 MANNVKK1025 pKa = 10.39 SDD1027 pKa = 3.33 GYY1029 pKa = 11.44 AGGLEE1034 pKa = 4.18 LGYY1037 pKa = 11.16 VLAQGNSWKK1046 pKa = 8.68 ITPQAQLTYY1055 pKa = 9.62 GTVDD1059 pKa = 3.29 YY1060 pKa = 11.53 NSFSDD1065 pKa = 4.02 GTSATAALADD1075 pKa = 3.69 GSVNSWRR1082 pKa = 11.84 GRR1084 pKa = 11.84 GGLEE1088 pKa = 4.92 LSNDD1092 pKa = 3.86 KK1093 pKa = 10.91 NWTSEE1098 pKa = 3.81 SGKK1101 pKa = 10.12 EE1102 pKa = 4.07 MSRR1105 pKa = 11.84 HH1106 pKa = 5.16 FYY1108 pKa = 10.85 GVLNLHH1114 pKa = 6.18 YY1115 pKa = 10.53 EE1116 pKa = 4.24 FDD1118 pKa = 4.17 GKK1120 pKa = 11.41 SNAAVSNTPLYY1131 pKa = 10.64 SRR1133 pKa = 11.84 PEE1135 pKa = 4.02 QLWGEE1140 pKa = 4.3 VGAGFQHH1147 pKa = 6.27 NAVGSISVFGEE1158 pKa = 3.52 AAYY1161 pKa = 10.53 SVSFANGGDD1170 pKa = 3.69 NNKK1173 pKa = 10.24 LNGRR1177 pKa = 11.84 IGLRR1181 pKa = 11.84 ANFF1184 pKa = 3.78
Molecular weight: 117.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.795
IPC_protein 3.859
Toseland 3.617
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.681
Grimsley 3.516
Solomon 3.872
Lehninger 3.834
Nozaki 3.973
DTASelect 4.304
Thurlkill 3.681
EMBOSS 3.846
Sillero 3.986
Patrickios 0.731
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|A0A6I4T5C2|A0A6I4T5C2_9SPHN Helix-turn-helix domain-containing protein OS=Altericroceibacterium endophyticum OX=1808508 GN=GRI91_06520 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.51 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.83 ATVGGRR28 pKa = 11.84 NVLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.97 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.46 NLCAA44 pKa = 4.54
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.369
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3079
0
3079
1041826
36
8661
338.4
36.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.886 ± 0.062
0.817 ± 0.014
6.356 ± 0.07
5.966 ± 0.041
3.713 ± 0.032
8.739 ± 0.097
1.941 ± 0.028
5.278 ± 0.031
3.22 ± 0.039
9.775 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.524 ± 0.028
2.983 ± 0.034
5.059 ± 0.039
3.515 ± 0.024
6.61 ± 0.053
5.939 ± 0.037
5.225 ± 0.036
6.605 ± 0.04
1.425 ± 0.025
2.424 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here