Papaya mosaic potexvirus (PMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|P20951|RDRP_PMV RNA replication protein OS=Papaya mosaic potexvirus OX=12181 PE=3 SV=1
MM1 pKa = 7.51VGVDD5 pKa = 3.44PEE7 pKa = 3.86IWPLVMKK14 pKa = 11.01AEE16 pKa = 4.38LLVKK20 pKa = 10.51LLSCAAAWKK29 pKa = 10.3RR30 pKa = 11.84CTCLLVALAIRR41 pKa = 11.84SSWVGDD47 pKa = 4.28LISPEE52 pKa = 4.48GGCMAAGFSTPSKK65 pKa = 10.3KK66 pKa = 10.72SNAANLALGLYY77 pKa = 9.83PEE79 pKa = 5.19ASQLAGAILSVLRR92 pKa = 11.84FQIIGAKK99 pKa = 9.41YY100 pKa = 7.91RR101 pKa = 11.84QNFLRR106 pKa = 11.84EE107 pKa = 3.97VPEE110 pKa = 4.21ALTILANCASDD121 pKa = 3.81ISGIDD126 pKa = 3.58DD127 pKa = 4.18GPVTVYY133 pKa = 11.24ALDD136 pKa = 4.11PLSS139 pKa = 3.6

Molecular weight:
14.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|P20955|Y14K_PMV Uncharacterized 14.1 kDa protein in ORF1 coding strand OS=Papaya mosaic potexvirus OX=12181 PE=4 SV=1
MM1 pKa = 6.97QWWNMKK7 pKa = 9.19TSSRR11 pKa = 11.84KK12 pKa = 9.26CLKK15 pKa = 10.4SMKK18 pKa = 10.49GKK20 pKa = 10.51FSQKK24 pKa = 8.59NTGTATVFKK33 pKa = 9.92PKK35 pKa = 9.87TPSFRR40 pKa = 11.84CFLTNRR46 pKa = 11.84QKK48 pKa = 8.19MTPYY52 pKa = 10.21FGLPLKK58 pKa = 9.99PDD60 pKa = 3.17WSSPTQRR67 pKa = 11.84PIGKK71 pKa = 8.3STLKK75 pKa = 10.14RR76 pKa = 11.84GQWEE80 pKa = 4.57KK81 pKa = 11.18CCLNHH86 pKa = 6.87TSGPCTCQRR95 pKa = 11.84CPFPLRR101 pKa = 11.84RR102 pKa = 11.84IFGTRR107 pKa = 11.84ACMRR111 pKa = 11.84CKK113 pKa = 10.47RR114 pKa = 11.84PTCPSLKK121 pKa = 9.63TT122 pKa = 3.5

Molecular weight:
14.11 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2435

68

1547

347.9

39.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.639 ± 1.076

2.012 ± 0.791

3.778 ± 0.431

6.776 ± 1.676

4.476 ± 0.333

5.462 ± 0.425

3.121 ± 0.678

5.462 ± 0.484

7.31 ± 1.413

9.815 ± 0.912

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.353

3.901 ± 0.344

6.817 ± 0.797

4.271 ± 0.447

4.476 ± 0.765

6.858 ± 1.645

6.283 ± 0.645

5.051 ± 0.62

1.355 ± 0.357

2.916 ± 0.498

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski