Papaya mosaic potexvirus (PMV)
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P20951|RDRP_PMV RNA replication protein OS=Papaya mosaic potexvirus OX=12181 PE=3 SV=1
MM1 pKa = 7.51 VGVDD5 pKa = 3.44 PEE7 pKa = 3.86 IWPLVMKK14 pKa = 11.01 AEE16 pKa = 4.38 LLVKK20 pKa = 10.51 LLSCAAAWKK29 pKa = 10.3 RR30 pKa = 11.84 CTCLLVALAIRR41 pKa = 11.84 SSWVGDD47 pKa = 4.28 LISPEE52 pKa = 4.48 GGCMAAGFSTPSKK65 pKa = 10.3 KK66 pKa = 10.72 SNAANLALGLYY77 pKa = 9.83 PEE79 pKa = 5.19 ASQLAGAILSVLRR92 pKa = 11.84 FQIIGAKK99 pKa = 9.41 YY100 pKa = 7.91 RR101 pKa = 11.84 QNFLRR106 pKa = 11.84 EE107 pKa = 3.97 VPEE110 pKa = 4.21 ALTILANCASDD121 pKa = 3.81 ISGIDD126 pKa = 3.58 DD127 pKa = 4.18 GPVTVYY133 pKa = 11.24 ALDD136 pKa = 4.11 PLSS139 pKa = 3.6
Molecular weight: 14.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.161
IPC2_protein 5.525
IPC_protein 5.245
Toseland 5.067
ProMoST 5.334
Dawson 5.181
Bjellqvist 5.321
Wikipedia 5.054
Rodwell 5.08
Grimsley 4.978
Solomon 5.181
Lehninger 5.13
Nozaki 5.296
DTASelect 5.436
Thurlkill 5.092
EMBOSS 5.067
Sillero 5.347
Patrickios 3.846
IPC_peptide 5.181
IPC2_peptide 5.347
IPC2.peptide.svr19 5.485
Protein with the highest isoelectric point:
>sp|P20955|Y14K_PMV Uncharacterized 14.1 kDa protein in ORF1 coding strand OS=Papaya mosaic potexvirus OX=12181 PE=4 SV=1
MM1 pKa = 6.97 QWWNMKK7 pKa = 9.19 TSSRR11 pKa = 11.84 KK12 pKa = 9.26 CLKK15 pKa = 10.4 SMKK18 pKa = 10.49 GKK20 pKa = 10.51 FSQKK24 pKa = 8.59 NTGTATVFKK33 pKa = 9.92 PKK35 pKa = 9.87 TPSFRR40 pKa = 11.84 CFLTNRR46 pKa = 11.84 QKK48 pKa = 8.19 MTPYY52 pKa = 10.21 FGLPLKK58 pKa = 9.99 PDD60 pKa = 3.17 WSSPTQRR67 pKa = 11.84 PIGKK71 pKa = 8.3 STLKK75 pKa = 10.14 RR76 pKa = 11.84 GQWEE80 pKa = 4.57 KK81 pKa = 11.18 CCLNHH86 pKa = 6.87 TSGPCTCQRR95 pKa = 11.84 CPFPLRR101 pKa = 11.84 RR102 pKa = 11.84 IFGTRR107 pKa = 11.84 ACMRR111 pKa = 11.84 CKK113 pKa = 10.47 RR114 pKa = 11.84 PTCPSLKK121 pKa = 9.63 TT122 pKa = 3.5
Molecular weight: 14.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.292
IPC2_protein 9.663
IPC_protein 9.867
Toseland 10.921
ProMoST 10.438
Dawson 10.965
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.433
Grimsley 10.979
Solomon 11.067
Lehninger 11.052
Nozaki 10.921
DTASelect 10.584
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.906
Patrickios 11.169
IPC_peptide 11.067
IPC2_peptide 9.867
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2435
68
1547
347.9
39.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.639 ± 1.076
2.012 ± 0.791
3.778 ± 0.431
6.776 ± 1.676
4.476 ± 0.333
5.462 ± 0.425
3.121 ± 0.678
5.462 ± 0.484
7.31 ± 1.413
9.815 ± 0.912
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.218 ± 0.353
3.901 ± 0.344
6.817 ± 0.797
4.271 ± 0.447
4.476 ± 0.765
6.858 ± 1.645
6.283 ± 0.645
5.051 ± 0.62
1.355 ± 0.357
2.916 ± 0.498
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here