Agathobacter rectalis (strain ATCC 33656 / DSM 3377 / JCM 17463 / KCTC 5835 / VPI 0990) (Eubacterium rectale)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae; [Eubacterium] rectale

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|C4ZHH4|Y1226_AGARV UPF0246 protein EUBREC_1226 OS=Agathobacter rectalis (strain ATCC 33656 / DSM 3377 / JCM 17463 / KCTC 5835 / VPI 0990) OX=515619 GN=EUBREC_1226 PE=3 SV=1
MM1 pKa = 7.33QIFVDD6 pKa = 4.63ADD8 pKa = 3.39ACPVVDD14 pKa = 3.7IVEE17 pKa = 5.09DD18 pKa = 3.3IAEE21 pKa = 4.14KK22 pKa = 10.89HH23 pKa = 6.09NIPVTLLGDD32 pKa = 4.02TNHH35 pKa = 5.86VLYY38 pKa = 10.7SDD40 pKa = 3.85YY41 pKa = 11.56SEE43 pKa = 4.79VIVVGSGADD52 pKa = 3.16AVDD55 pKa = 3.47YY56 pKa = 10.87KK57 pKa = 10.91LISICTVII65 pKa = 4.67

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4ZFW5|C4ZFW5_AGARV ABC transporter ATP-binding protein OS=Agathobacter rectalis (strain ATCC 33656 / DSM 3377 / JCM 17463 / KCTC 5835 / VPI 0990) OX=515619 GN=EUBREC_0935 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.96VHH16 pKa = 6.01GFRR19 pKa = 11.84QRR21 pKa = 11.84MKK23 pKa = 8.6TAGGRR28 pKa = 11.84KK29 pKa = 8.92VIAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.55GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3545

0

3545

1016923

39

2562

286.9

32.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.264 ± 0.049

1.52 ± 0.017

6.345 ± 0.041

7.056 ± 0.05

4.139 ± 0.029

6.612 ± 0.039

1.728 ± 0.018

7.86 ± 0.039

7.394 ± 0.041

8.346 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.145 ± 0.021

4.942 ± 0.033

2.959 ± 0.023

3.074 ± 0.024

4.051 ± 0.038

6.096 ± 0.032

5.418 ± 0.036

6.788 ± 0.043

0.875 ± 0.016

4.388 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski