Acinetobacter calcoaceticus (strain PHEA-2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii complex; Acinetobacter pittii

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3598 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0KPV5|F0KPV5_ACICP Uncharacterized protein OS=Acinetobacter calcoaceticus (strain PHEA-2) OX=871585 GN=BDGL_002464 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 9.67IDD4 pKa = 4.35NISYY8 pKa = 10.39EE9 pKa = 4.12YY10 pKa = 11.02DD11 pKa = 3.63DD12 pKa = 5.93LIKK15 pKa = 11.23LNITDD20 pKa = 3.87TKK22 pKa = 10.61EE23 pKa = 4.01IEE25 pKa = 5.06CMTLLNKK32 pKa = 9.88LDD34 pKa = 3.69AMTPILTNLFSYY46 pKa = 10.61DD47 pKa = 3.15VSTDD51 pKa = 3.52KK52 pKa = 10.96IIFNPPSDD60 pKa = 3.81SYY62 pKa = 11.3IVEE65 pKa = 4.12QKK67 pKa = 10.63VDD69 pKa = 3.74NTWVQINLEE78 pKa = 4.33EE79 pKa = 4.75KK80 pKa = 10.45FDD82 pKa = 3.78WLNTEE87 pKa = 4.28FRR89 pKa = 11.84VTTTDD94 pKa = 2.94QAGNISQPLITIINTASGTYY114 pKa = 9.93KK115 pKa = 9.83PTDD118 pKa = 3.34TTSNQMVKK126 pKa = 10.72GSTGNDD132 pKa = 3.24YY133 pKa = 11.18LYY135 pKa = 11.19GGDD138 pKa = 4.67GNDD141 pKa = 3.46TLISNTGSDD150 pKa = 3.97YY151 pKa = 11.18LYY153 pKa = 11.1GGAGNDD159 pKa = 3.44TLVYY163 pKa = 10.35GGNSNGYY170 pKa = 7.18TALLGQAGNDD180 pKa = 3.37TYY182 pKa = 11.03IIDD185 pKa = 3.99KK186 pKa = 10.73VLLSSLSYY194 pKa = 10.81VHH196 pKa = 7.18ILDD199 pKa = 4.2NSTEE203 pKa = 4.05EE204 pKa = 3.78NTLQLKK210 pKa = 9.99SVSADD215 pKa = 3.59EE216 pKa = 5.62IILKK220 pKa = 10.17QSNTDD225 pKa = 3.49IIITFNDD232 pKa = 3.18STATIHH238 pKa = 6.64FGEE241 pKa = 4.81GQLSAIVFDD250 pKa = 6.39DD251 pKa = 3.7GTVWNKK257 pKa = 10.2AQIEE261 pKa = 4.33ANTIGKK267 pKa = 9.36LLGNDD272 pKa = 3.3ADD274 pKa = 5.96DD275 pKa = 4.21YY276 pKa = 11.03LQADD280 pKa = 4.15AEE282 pKa = 4.2ISTIYY287 pKa = 10.52GLEE290 pKa = 4.28GNDD293 pKa = 3.79TIQGGVQNDD302 pKa = 3.67YY303 pKa = 11.38LYY305 pKa = 11.32GGDD308 pKa = 4.67GNDD311 pKa = 3.46TLISNTGTDD320 pKa = 3.72YY321 pKa = 11.44LYY323 pKa = 11.29GGAGNDD329 pKa = 3.43TLIYY333 pKa = 10.23GGNSNGYY340 pKa = 7.18TALLGQAGNDD350 pKa = 3.48TYY352 pKa = 11.16IVDD355 pKa = 4.0KK356 pKa = 10.98ALLSSWSYY364 pKa = 10.37VHH366 pKa = 7.25ILDD369 pKa = 4.76NSTEE373 pKa = 4.0EE374 pKa = 5.1NILQLKK380 pKa = 9.78SVLADD385 pKa = 4.01EE386 pKa = 5.82IILKK390 pKa = 10.3KK391 pKa = 10.85SNADD395 pKa = 3.44IIITFNDD402 pKa = 3.18STATIHH408 pKa = 6.84FGEE411 pKa = 4.52EE412 pKa = 3.9QLSSIVFDD420 pKa = 6.24DD421 pKa = 3.79GTVWNKK427 pKa = 10.2AQIEE431 pKa = 4.33ANTIGKK437 pKa = 9.33LLGTDD442 pKa = 3.22ADD444 pKa = 5.25DD445 pKa = 4.05YY446 pKa = 11.13LQADD450 pKa = 3.86AEE452 pKa = 4.47MKK454 pKa = 10.74NNYY457 pKa = 9.19LLLYY461 pKa = 9.62LTMEE465 pKa = 3.91QSGIKK470 pKa = 10.19LKK472 pKa = 10.82LKK474 pKa = 10.53RR475 pKa = 11.84ILLANYY481 pKa = 9.33

Molecular weight: 52.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0KPV4|F0KPV4_ACICP ClpXP protease specificity-enhancing factor OS=Acinetobacter calcoaceticus (strain PHEA-2) OX=871585 GN=sspB PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.55RR3 pKa = 11.84TFQPSEE9 pKa = 3.97LKK11 pKa = 10.13RR12 pKa = 11.84KK13 pKa = 8.98RR14 pKa = 11.84VHH16 pKa = 6.36GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4AGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84HH40 pKa = 5.1SLTVV44 pKa = 3.06

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3598

0

3598

1122070

37

4331

311.9

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.608 ± 0.049

0.934 ± 0.013

5.066 ± 0.033

5.862 ± 0.047

4.34 ± 0.034

6.713 ± 0.047

2.345 ± 0.023

6.855 ± 0.04

5.609 ± 0.037

10.473 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.025

4.485 ± 0.044

4.021 ± 0.029

5.268 ± 0.048

4.191 ± 0.037

6.141 ± 0.031

5.41 ± 0.042

6.733 ± 0.042

1.279 ± 0.016

3.27 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski