Hamster polyomavirus (HaPyV) (Mesocricetus auratus polyomavirus 1)
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q84255|Q84255_POVHA Capsid protein VP1 OS=Hamster polyomavirus OX=1891729 GN=VP1 PE=3 SV=1
MM1 pKa = 7.51 GSAISVIIEE10 pKa = 4.14 MISYY14 pKa = 10.09 LSEE17 pKa = 3.78 ISSVTGISVEE27 pKa = 4.46 AILSGEE33 pKa = 3.91 AFAAIDD39 pKa = 3.7 AQVTSLITMEE49 pKa = 4.51 GFLGAEE55 pKa = 4.29 TALSSIGLSEE65 pKa = 4.76 DD66 pKa = 3.07 MFIFMQAAPEE76 pKa = 4.07 LTSTVMTEE84 pKa = 4.26 FVRR87 pKa = 11.84 EE88 pKa = 4.16 SVQTAFIFQTVAGSAAFSLGSLHH111 pKa = 7.19 GYY113 pKa = 8.7 LAHH116 pKa = 6.8 EE117 pKa = 4.35 VPIVNRR123 pKa = 11.84 NMALIPRR130 pKa = 11.84 RR131 pKa = 11.84 PADD134 pKa = 3.37 YY135 pKa = 11.21 YY136 pKa = 11.28 DD137 pKa = 3.24 ILFPGVQSFTHH148 pKa = 6.32 ALDD151 pKa = 4.59 VIHH154 pKa = 6.89 GWGHH158 pKa = 6.21 SLFQSVGEE166 pKa = 4.51 YY167 pKa = 9.89 IWDD170 pKa = 3.59 TLRR173 pKa = 11.84 RR174 pKa = 11.84 EE175 pKa = 4.04 TQGAVEE181 pKa = 4.18 SAVRR185 pKa = 11.84 DD186 pKa = 3.53 LSLQTTHH193 pKa = 6.35 QFLDD197 pKa = 4.54 AIARR201 pKa = 11.84 MMEE204 pKa = 3.69 NSRR207 pKa = 11.84 WVVTNLPRR215 pKa = 11.84 EE216 pKa = 4.3 AYY218 pKa = 9.57 SRR220 pKa = 11.84 IYY222 pKa = 10.87 GGLQNYY228 pKa = 7.98 YY229 pKa = 11.09 AEE231 pKa = 4.8 LPGINPAQRR240 pKa = 11.84 RR241 pKa = 11.84 QIEE244 pKa = 4.03 RR245 pKa = 11.84 ALEE248 pKa = 3.9 YY249 pKa = 10.87 SNRR252 pKa = 11.84 PSIEE256 pKa = 4.13 DD257 pKa = 3.37 ANSRR261 pKa = 11.84 QVLEE265 pKa = 4.66 AEE267 pKa = 4.54 LGRR270 pKa = 11.84 PDD272 pKa = 3.34 VQRR275 pKa = 11.84 RR276 pKa = 11.84 SQQQEE281 pKa = 4.04 DD282 pKa = 3.78 SSSWFEE288 pKa = 3.84 SGANIMRR295 pKa = 11.84 YY296 pKa = 8.22 FAPGGAHH303 pKa = 6.28 QRR305 pKa = 11.84 VTPDD309 pKa = 2.33 WMLPLILGLYY319 pKa = 10.31 GDD321 pKa = 5.41 ISPTWQTYY329 pKa = 8.91 IDD331 pKa = 3.8 EE332 pKa = 4.49 VEE334 pKa = 4.13 YY335 pKa = 11.09 GPKK338 pKa = 9.45 KK339 pKa = 9.75 KK340 pKa = 9.97 KK341 pKa = 10.14 RR342 pKa = 11.84 RR343 pKa = 11.84 FQQ345 pKa = 3.43
Molecular weight: 38.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.951
IPC2_protein 5.003
IPC_protein 4.927
Toseland 4.863
ProMoST 5.041
Dawson 4.902
Bjellqvist 5.029
Wikipedia 4.749
Rodwell 4.825
Grimsley 4.787
Solomon 4.902
Lehninger 4.851
Nozaki 5.016
DTASelect 5.13
Thurlkill 4.851
EMBOSS 4.787
Sillero 5.092
Patrickios 3.91
IPC_peptide 4.914
IPC2_peptide 5.092
IPC2.peptide.svr19 5.039
Protein with the highest isoelectric point:
>sp|P03080|ST_POVHA Small t antigen OS=Hamster polyomavirus OX=1891729 PE=3 SV=1
MM1 pKa = 7.89 DD2 pKa = 6.22 RR3 pKa = 11.84 ILTKK7 pKa = 9.85 EE8 pKa = 4.18 EE9 pKa = 3.69 KK10 pKa = 9.88 QALISLLDD18 pKa = 4.39 LEE20 pKa = 4.3 PQYY23 pKa = 10.79 WGDD26 pKa = 3.48 YY27 pKa = 11.05 GRR29 pKa = 11.84 MQKK32 pKa = 10.3 CYY34 pKa = 10.37 KK35 pKa = 10.05 KK36 pKa = 10.45 KK37 pKa = 10.28 CLQLHH42 pKa = 6.36 PDD44 pKa = 3.03 KK45 pKa = 11.37 GGNEE49 pKa = 3.96 EE50 pKa = 6.16 LMQQLNTLWTKK61 pKa = 10.95 LKK63 pKa = 10.75 DD64 pKa = 3.18 GLYY67 pKa = 9.77 RR68 pKa = 11.84 VRR70 pKa = 11.84 LLLGPSQVRR79 pKa = 11.84 RR80 pKa = 11.84 LGKK83 pKa = 9.84 DD84 pKa = 2.79 QWNLSLQQTFSGTYY98 pKa = 8.77 FRR100 pKa = 11.84 RR101 pKa = 11.84 LCRR104 pKa = 11.84 LPITCLRR111 pKa = 11.84 NKK113 pKa = 10.41 GISTCNCILCLLRR126 pKa = 11.84 KK127 pKa = 8.81 QHH129 pKa = 6.69 FLLKK133 pKa = 10.37 KK134 pKa = 8.75 SWRR137 pKa = 11.84 VPCLVLGEE145 pKa = 5.08 CYY147 pKa = 10.58 CIDD150 pKa = 5.31 CFALWFGLPVTNMLVPLYY168 pKa = 10.31 AQFLAPIPVDD178 pKa = 3.1 WLDD181 pKa = 4.15 LNVHH185 pKa = 5.84 EE186 pKa = 5.29 VYY188 pKa = 10.96 NPASGMTLMLPPPPADD204 pKa = 3.98 PEE206 pKa = 4.34 SSTILTQEE214 pKa = 4.03 DD215 pKa = 3.87 TGPTLMGQQDD225 pKa = 4.16 TLTSRR230 pKa = 11.84 RR231 pKa = 11.84 NTGKK235 pKa = 10.31 SFSLSGMLMRR245 pKa = 11.84 TSPAKK250 pKa = 10.16 KK251 pKa = 9.8 SYY253 pKa = 9.77 HH254 pKa = 4.81 HH255 pKa = 6.48 QKK257 pKa = 9.7 MNSPPGIPIPPPPLFLFPVTAPVPPVTRR285 pKa = 11.84 NTQEE289 pKa = 3.88 TQAEE293 pKa = 4.29 RR294 pKa = 11.84 EE295 pKa = 4.04 NEE297 pKa = 4.3 YY298 pKa = 10.52 MPMAPQIHH306 pKa = 7.42 LYY308 pKa = 9.4 SQIRR312 pKa = 11.84 EE313 pKa = 4.13 PTHH316 pKa = 6.39 QEE318 pKa = 3.68 EE319 pKa = 4.8 EE320 pKa = 4.21 EE321 pKa = 4.15 PQYY324 pKa = 11.37 EE325 pKa = 4.44 EE326 pKa = 3.79 IPIYY330 pKa = 10.95 LEE332 pKa = 4.32 LLPEE336 pKa = 4.49 NPNQHH341 pKa = 6.8 LALTSTARR349 pKa = 11.84 RR350 pKa = 11.84 SLRR353 pKa = 11.84 RR354 pKa = 11.84 KK355 pKa = 6.76 YY356 pKa = 10.04 HH357 pKa = 5.32 KK358 pKa = 10.1 HH359 pKa = 5.24 NSHH362 pKa = 7.6 IITQRR367 pKa = 11.84 QRR369 pKa = 11.84 NRR371 pKa = 11.84 LRR373 pKa = 11.84 RR374 pKa = 11.84 LVLMIFLLSLGGFFLTLFFLIKK396 pKa = 10.35 RR397 pKa = 11.84 KK398 pKa = 8.21 MHH400 pKa = 6.39 LL401 pKa = 3.49
Molecular weight: 46.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.817
IPC2_protein 8.682
IPC_protein 8.595
Toseland 9.107
ProMoST 9.077
Dawson 9.487
Bjellqvist 9.385
Wikipedia 9.648
Rodwell 9.648
Grimsley 9.502
Solomon 9.531
Lehninger 9.487
Nozaki 9.545
DTASelect 9.268
Thurlkill 9.37
EMBOSS 9.619
Sillero 9.545
Patrickios 4.66
IPC_peptide 9.516
IPC2_peptide 8.317
IPC2.peptide.svr19 7.791
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
1
6
2284
194
751
380.7
42.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.429 ± 0.821
2.145 ± 0.654
4.641 ± 0.448
6.305 ± 0.522
3.722 ± 0.589
6.13 ± 0.787
2.189 ± 0.282
4.51 ± 0.644
5.648 ± 1.197
10.814 ± 1.321
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.671 ± 0.263
4.466 ± 0.514
6.173 ± 0.586
5.385 ± 0.419
5.473 ± 0.834
6.524 ± 0.75
5.648 ± 0.48
5.385 ± 0.601
1.751 ± 0.23
4.072 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here