Circoviridae 6 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TNC2|S5TNC2_9CIRC Replication-associated protein OS=Circoviridae 6 LDMD-2013 OX=1379710 PE=4 SV=1
MM1 pKa = 7.68SDD3 pKa = 3.25VPAAPPSTVAAGRR16 pKa = 11.84SRR18 pKa = 11.84GWTGSMFDD26 pKa = 3.62YY27 pKa = 11.13DD28 pKa = 4.92DD29 pKa = 4.35VYY31 pKa = 11.59EE32 pKa = 4.43SLCEE36 pKa = 3.79ALAAAVSEE44 pKa = 4.11QRR46 pKa = 11.84VTRR49 pKa = 11.84AILGKK54 pKa = 8.23EE55 pKa = 4.06VCPTTQRR62 pKa = 11.84KK63 pKa = 7.77HH64 pKa = 5.05LQWYY68 pKa = 9.29VEE70 pKa = 4.37FGNAVQRR77 pKa = 11.84GSVTNLGITGHH88 pKa = 5.25TEE90 pKa = 3.42HH91 pKa = 7.51RR92 pKa = 11.84LGSPAQCWQYY102 pKa = 11.05CSKK105 pKa = 11.11DD106 pKa = 2.99GDD108 pKa = 3.85FATFGSPPKK117 pKa = 10.4GQGKK121 pKa = 10.15RR122 pKa = 11.84SDD124 pKa = 3.6LDD126 pKa = 3.41QAIEE130 pKa = 4.11TLTSEE135 pKa = 4.32GLEE138 pKa = 4.1ATAMEE143 pKa = 4.68HH144 pKa = 6.05PAAFVKK150 pKa = 10.62YY151 pKa = 10.04GRR153 pKa = 11.84GLAALEE159 pKa = 4.05ARR161 pKa = 11.84FTRR164 pKa = 11.84PFPLPQPTSPPRR176 pKa = 11.84YY177 pKa = 6.68VTPVRR182 pKa = 11.84VRR184 pKa = 11.84LAEE187 pKa = 3.95YY188 pKa = 8.92PQEE191 pKa = 5.51AEE193 pKa = 4.66APPPTPRR200 pKa = 11.84RR201 pKa = 11.84HH202 pKa = 5.68GGSGRR207 pKa = 11.84GCVVGPDD214 pKa = 4.15GNWEE218 pKa = 3.83IPYY221 pKa = 9.58GVPGGIGSVCMGTRR235 pKa = 11.84DD236 pKa = 3.28GSMVV240 pKa = 3.03

Molecular weight: 25.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5SYC6|S5SYC6_9CIRC Replication-associated protein OS=Circoviridae 6 LDMD-2013 OX=1379710 PE=4 SV=1
MM1 pKa = 7.8PLRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84PVAMRR11 pKa = 11.84APRR14 pKa = 11.84PSLTAMRR21 pKa = 11.84ALGIPLRR28 pKa = 11.84AAPYY32 pKa = 8.21VARR35 pKa = 11.84AAGAVPGIAVAGLANLYY52 pKa = 9.79QSYY55 pKa = 9.65RR56 pKa = 11.84ATEE59 pKa = 3.77DD60 pKa = 2.85HH61 pKa = 6.64MARR64 pKa = 11.84TLKK67 pKa = 10.36RR68 pKa = 11.84GRR70 pKa = 11.84STYY73 pKa = 10.96GGGSSAKK80 pKa = 9.94KK81 pKa = 8.96YY82 pKa = 10.04KK83 pKa = 10.26KK84 pKa = 9.97PNGSRR89 pKa = 11.84WRR91 pKa = 11.84PGARR95 pKa = 11.84NWTSRR100 pKa = 11.84RR101 pKa = 11.84KK102 pKa = 9.52VAAKK106 pKa = 10.28LSAVEE111 pKa = 4.28TKK113 pKa = 10.39ISPLFRR119 pKa = 11.84RR120 pKa = 11.84EE121 pKa = 3.87DD122 pKa = 3.4VAATPFPTPDD132 pKa = 2.88TGNVVKK138 pKa = 9.96TGQDD142 pKa = 3.51LLTAAHH148 pKa = 6.25FVIGSGLPAAKK159 pKa = 9.79LQWYY163 pKa = 7.85TGVEE167 pKa = 4.21STATSITHH175 pKa = 6.07SLGGMFFPPAQHH187 pKa = 5.56TTGTDD192 pKa = 3.5EE193 pKa = 4.24VPSYY197 pKa = 10.7ADD199 pKa = 3.15RR200 pKa = 11.84MIRR203 pKa = 11.84NGRR206 pKa = 11.84SIYY209 pKa = 10.44LRR211 pKa = 11.84RR212 pKa = 11.84TSIAVHH218 pKa = 5.32ITCPTVQASGNLEE231 pKa = 4.15LNEE234 pKa = 4.41AGPFRR239 pKa = 11.84VRR241 pKa = 11.84MIVYY245 pKa = 9.49RR246 pKa = 11.84VRR248 pKa = 11.84LATTQSEE255 pKa = 4.38DD256 pKa = 3.37AEE258 pKa = 4.23EE259 pKa = 4.89RR260 pKa = 11.84DD261 pKa = 3.3TGKK264 pKa = 10.54DD265 pKa = 3.45FFIDD269 pKa = 3.41PMGRR273 pKa = 11.84PWGWKK278 pKa = 10.34GFDD281 pKa = 4.12DD282 pKa = 5.07QVKK285 pKa = 9.77QPDD288 pKa = 3.45THH290 pKa = 7.22LPRR293 pKa = 11.84ITGYY297 pKa = 11.01DD298 pKa = 3.61VMSQPVNSNKK308 pKa = 9.9YY309 pKa = 8.28QVIKK313 pKa = 10.46DD314 pKa = 3.87VKK316 pKa = 10.62FSMQPKK322 pKa = 8.43MSISAAEE329 pKa = 4.1SDD331 pKa = 3.76TTGQWLVNTPKK342 pKa = 10.55RR343 pKa = 11.84APHH346 pKa = 5.48TKK348 pKa = 9.95YY349 pKa = 10.87FNIQLPWGRR358 pKa = 11.84KK359 pKa = 6.6AHH361 pKa = 5.48FTNTITGAQEE371 pKa = 3.35DD372 pKa = 4.62TLALRR377 pKa = 11.84HH378 pKa = 5.63AAMMQEE384 pKa = 4.31SCDD387 pKa = 3.71FSPILKK393 pKa = 10.58SIDD396 pKa = 3.36LSAAEE401 pKa = 4.67PDD403 pKa = 3.56DD404 pKa = 4.61FNWRR408 pKa = 11.84THH410 pKa = 4.92IVFFGGPQRR419 pKa = 11.84GAGRR423 pKa = 11.84FTGTWEE429 pKa = 3.56PAITIRR435 pKa = 11.84GMTRR439 pKa = 11.84AYY441 pKa = 10.47DD442 pKa = 3.33AA443 pKa = 5.03

Molecular weight: 49.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

862

179

443

287.3

31.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.397 ± 1.306

1.16 ± 0.565

4.524 ± 0.314

4.408 ± 0.796

3.712 ± 0.327

8.817 ± 1.093

2.784 ± 0.71

3.712 ± 0.76

4.06 ± 0.617

6.729 ± 1.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.784 ± 0.298

2.204 ± 0.516

7.541 ± 0.84

4.06 ± 0.362

8.237 ± 0.401

6.497 ± 0.123

7.425 ± 1.11

6.497 ± 0.758

2.32 ± 0.503

3.132 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski