Mesocricetus auratus papillomavirus 1
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U6ELS6|U6ELS6_9PAPI Protein E7 OS=Mesocricetus auratus papillomavirus 1 OX=1408129 GN=E7 PE=3 SV=1
MM1 pKa = 6.87 VAANRR6 pKa = 11.84 ARR8 pKa = 11.84 RR9 pKa = 11.84 VKK11 pKa = 10.38 RR12 pKa = 11.84 DD13 pKa = 3.24 SAEE16 pKa = 3.68 NLYY19 pKa = 10.02 RR20 pKa = 11.84 QCQITGNCPPDD31 pKa = 3.47 VVNKK35 pKa = 10.28 VEE37 pKa = 4.13 GTTLADD43 pKa = 3.33 KK44 pKa = 9.14 LTKK47 pKa = 10.05 IFASILYY54 pKa = 10.19 LGGLGIGTGRR64 pKa = 11.84 GTGGATGYY72 pKa = 11.23 GPINPGGGRR81 pKa = 11.84 ITGTGTVMRR90 pKa = 11.84 PGVVIEE96 pKa = 4.19 PVGPGDD102 pKa = 3.56 IVSVGATDD110 pKa = 3.67 SSIVPLLEE118 pKa = 4.09 ATPDD122 pKa = 3.28 IPIEE126 pKa = 4.48 GGPEE130 pKa = 3.79 VPPAGPDD137 pKa = 3.19 VSTVDD142 pKa = 3.03 VTANVDD148 pKa = 4.11 PISDD152 pKa = 3.69 INVTSSTTITNPDD165 pKa = 3.32 SAVIDD170 pKa = 3.93 VQPAPSGPRR179 pKa = 11.84 RR180 pKa = 11.84 VTVSRR185 pKa = 11.84 SEE187 pKa = 4.07 FQNASYY193 pKa = 11.39 VSVTHH198 pKa = 6.8 PSQGLGEE205 pKa = 4.33 SGGAVISADD214 pKa = 3.1 AGGSVVSSSHH224 pKa = 5.69 EE225 pKa = 3.95 LDD227 pKa = 3.23 VGILIGGRR235 pKa = 11.84 PPGNLSEE242 pKa = 4.23 FQTIEE247 pKa = 3.89 LDD249 pKa = 4.73 EE250 pKa = 4.4 IVQTQGVDD258 pKa = 3.23 DD259 pKa = 4.27 FDD261 pKa = 4.56 IGEE264 pKa = 4.42 GAGRR268 pKa = 11.84 AGPDD272 pKa = 2.79 TSTPDD277 pKa = 3.23 NYY279 pKa = 10.81 LGRR282 pKa = 11.84 ALSRR286 pKa = 11.84 FRR288 pKa = 11.84 EE289 pKa = 4.31 IYY291 pKa = 9.64 EE292 pKa = 4.33 RR293 pKa = 11.84 PSDD296 pKa = 3.7 LYY298 pKa = 9.96 TRR300 pKa = 11.84 RR301 pKa = 11.84 VQQVRR306 pKa = 11.84 VRR308 pKa = 11.84 NRR310 pKa = 11.84 DD311 pKa = 3.25 LFLGDD316 pKa = 3.54 PGRR319 pKa = 11.84 LVTYY323 pKa = 10.03 EE324 pKa = 3.81 FDD326 pKa = 3.52 NPAFEE331 pKa = 5.13 GLGEE335 pKa = 4.03 DD336 pKa = 4.41 TIIFHH341 pKa = 6.45 QAPEE345 pKa = 4.13 VQAAPDD351 pKa = 4.09 EE352 pKa = 4.47 DD353 pKa = 4.3 FMDD356 pKa = 4.9 IIKK359 pKa = 10.7 LGRR362 pKa = 11.84 QRR364 pKa = 11.84 LAQTPGGTVRR374 pKa = 11.84 ISRR377 pKa = 11.84 LGQRR381 pKa = 11.84 GTIRR385 pKa = 11.84 TRR387 pKa = 11.84 SGLQIGGKK395 pKa = 7.39 VHH397 pKa = 6.87 FFSDD401 pKa = 4.05 LSPIATEE408 pKa = 4.38 NIEE411 pKa = 4.18 LSPLGEE417 pKa = 3.97 ISGEE421 pKa = 3.98 AMVVDD426 pKa = 4.58 ALSEE430 pKa = 3.98 YY431 pKa = 11.25 SLISEE436 pKa = 4.63 PGIEE440 pKa = 4.44 PVPFMDD446 pKa = 5.44 EE447 pKa = 4.32 DD448 pKa = 3.97 LLDD451 pKa = 3.85 IQSEE455 pKa = 4.34 DD456 pKa = 3.57 FSGSRR461 pKa = 11.84 LHH463 pKa = 7.19 LVRR466 pKa = 11.84 TGNRR470 pKa = 11.84 FGVIYY475 pKa = 10.06 EE476 pKa = 3.9 PSEE479 pKa = 3.78 NLGARR484 pKa = 11.84 TVFPDD489 pKa = 3.15 INTGDD494 pKa = 4.9 FIRR497 pKa = 11.84 YY498 pKa = 5.82 PQSNINPADD507 pKa = 3.29 ISYY510 pKa = 11.08 QDD512 pKa = 5.66 LIPLQPGVDD521 pKa = 3.35 INVGSIYY528 pKa = 10.24 PSVDD532 pKa = 3.37 YY533 pKa = 10.69 YY534 pKa = 11.14 LHH536 pKa = 6.65 PSLRR540 pKa = 11.84 RR541 pKa = 11.84 RR542 pKa = 11.84 KK543 pKa = 9.68 RR544 pKa = 11.84 KK545 pKa = 8.56 RR546 pKa = 11.84 TLHH549 pKa = 5.67
Molecular weight: 58.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.752
IPC2_protein 4.698
IPC_protein 4.66
Toseland 4.507
ProMoST 4.774
Dawson 4.622
Bjellqvist 4.774
Wikipedia 4.507
Rodwell 4.507
Grimsley 4.418
Solomon 4.622
Lehninger 4.584
Nozaki 4.736
DTASelect 4.927
Thurlkill 4.52
EMBOSS 4.533
Sillero 4.787
Patrickios 4.101
IPC_peptide 4.635
IPC2_peptide 4.774
IPC2.peptide.svr19 4.759
Protein with the highest isoelectric point:
>tr|U6ELD1|U6ELD1_9PAPI Minor capsid protein L2 OS=Mesocricetus auratus papillomavirus 1 OX=1408129 GN=L2 PE=3 SV=1
MM1 pKa = 7.85 AFPIEE6 pKa = 4.4 LPALIQLLNTDD17 pKa = 3.1 VTRR20 pKa = 11.84 IVLYY24 pKa = 10.74 CLFCDD29 pKa = 5.04 RR30 pKa = 11.84 PLSMQDD36 pKa = 2.65 KK37 pKa = 10.15 FDD39 pKa = 3.8 YY40 pKa = 10.76 FGAGLKK46 pKa = 9.02 VVCRR50 pKa = 11.84 KK51 pKa = 10.17 GGMRR55 pKa = 11.84 IACMSCRR62 pKa = 11.84 RR63 pKa = 11.84 ALAYY67 pKa = 10.4 AEE69 pKa = 4.47 DD70 pKa = 3.99 LRR72 pKa = 11.84 HH73 pKa = 5.57 RR74 pKa = 11.84 QCTAEE79 pKa = 4.12 GDD81 pKa = 3.73 LVEE84 pKa = 5.66 RR85 pKa = 11.84 ITGKK89 pKa = 10.35 SLLHH93 pKa = 6.68 IDD95 pKa = 3.88 VRR97 pKa = 11.84 CIACLCRR104 pKa = 11.84 LGPSEE109 pKa = 4.8 KK110 pKa = 10.52 LLAKK114 pKa = 10.35 ASHH117 pKa = 6.14 KK118 pKa = 10.22 PFYY121 pKa = 10.34 LVRR124 pKa = 11.84 SLWRR128 pKa = 11.84 GPCRR132 pKa = 11.84 KK133 pKa = 9.75 CFLLL137 pKa = 5.23
Molecular weight: 15.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.503
IPC2_protein 8.317
IPC_protein 8.185
Toseland 7.819
ProMoST 8.726
Dawson 8.887
Bjellqvist 9.268
Wikipedia 8.829
Rodwell 8.931
Grimsley 7.688
Solomon 9.019
Lehninger 9.033
Nozaki 9.575
DTASelect 8.946
Thurlkill 9.019
EMBOSS 9.092
Sillero 9.355
Patrickios 4.406
IPC_peptide 9.004
IPC2_peptide 8.726
IPC2.peptide.svr19 8.584
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2514
137
608
359.1
40.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.444 ± 0.582
2.426 ± 0.707
6.762 ± 0.524
5.688 ± 0.643
4.097 ± 0.551
7.08 ± 1.129
1.671 ± 0.12
4.574 ± 0.797
4.733 ± 0.819
9.109 ± 0.623
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.71 ± 0.225
3.699 ± 0.62
7.518 ± 1.241
4.375 ± 0.566
6.484 ± 0.636
7.16 ± 0.659
5.927 ± 0.442
6.086 ± 0.749
1.034 ± 0.309
3.421 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here