Acyrthosiphon pisum virus
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O55320|O55320_9VIRU Protein P2 (Fragment) OS=Acyrthosiphon pisum virus OX=340187 PE=4 SV=1
CC1 pKa = 7.82 KK2 pKa = 9.91 EE3 pKa = 3.7 RR4 pKa = 11.84 LFFKK8 pKa = 10.93 LLAPQLSQRR17 pKa = 11.84 LRR19 pKa = 11.84 GASLGNIFGLAYY31 pKa = 9.28 TDD33 pKa = 3.8 YY34 pKa = 10.56 IYY36 pKa = 11.43 LHH38 pKa = 6.98 IEE40 pKa = 3.9 GDD42 pKa = 3.68 KK43 pKa = 10.77 LTDD46 pKa = 4.25 LLFQNALTNIPDD58 pKa = 4.09 FSTISLLQHH67 pKa = 6.76 EE68 pKa = 4.87 ISSTEE73 pKa = 3.5 SGYY76 pKa = 8.88 TFQFCDD82 pKa = 3.04 AGYY85 pKa = 11.04 ASYY88 pKa = 10.31 YY89 pKa = 9.45 QNSSHH94 pKa = 7.09 FVLFQSNSNNFLPLIKK110 pKa = 10.07 DD111 pKa = 3.61 YY112 pKa = 11.53 KK113 pKa = 10.99 NNAEE117 pKa = 4.08 YY118 pKa = 11.21 ADD120 pKa = 3.9 ISRR123 pKa = 11.84 TIYY126 pKa = 10.47 DD127 pKa = 4.41 WYY129 pKa = 11.02 NPTYY133 pKa = 10.87 DD134 pKa = 4.14 EE135 pKa = 5.16 DD136 pKa = 4.78 DD137 pKa = 3.48 YY138 pKa = 11.8 CIFYY142 pKa = 10.64 KK143 pKa = 10.57 VEE145 pKa = 3.89 QVNKK149 pKa = 9.72 FFIEE153 pKa = 4.3 KK154 pKa = 9.72 PATCVAHH161 pKa = 4.99 TTFTGSKK168 pKa = 9.67 AEE170 pKa = 4.3 WIFTPEE176 pKa = 3.59 AFEE179 pKa = 3.95 VVEE182 pKa = 4.99 FIKK185 pKa = 10.86 DD186 pKa = 3.3 RR187 pKa = 11.84 VKK189 pKa = 10.96 FRR191 pKa = 11.84 VTKK194 pKa = 10.64 LLLPKK199 pKa = 9.32 TQEE202 pKa = 3.49 RR203 pKa = 11.84 DD204 pKa = 3.26 MSFKK208 pKa = 11.07 NLISFDD214 pKa = 4.12 LNYY217 pKa = 10.12 PAINQIEE224 pKa = 4.25 KK225 pKa = 10.56 ALNNVIPANLPKK237 pKa = 10.62 GIRR240 pKa = 11.84 QIVFSSYY247 pKa = 11.51 AQFSVNNPYY256 pKa = 10.35 RR257 pKa = 11.84 GRR259 pKa = 11.84 QTPHH263 pKa = 6.78 IAPIYY268 pKa = 10.1 LFISRR273 pKa = 11.84 LKK275 pKa = 8.77 QQYY278 pKa = 9.77 GSEE281 pKa = 4.14 TVEE284 pKa = 4.28 IIFNSAFNGQQVIAARR300 pKa = 11.84 IDD302 pKa = 3.52 LSTAVEE308 pKa = 3.95 NTFIYY313 pKa = 10.27 IKK315 pKa = 9.94 TVDD318 pKa = 3.91 EE319 pKa = 4.58 APLYY323 pKa = 8.95 STLRR327 pKa = 11.84 FPSAMLEE334 pKa = 4.03 LLSIQTVYY342 pKa = 10.96 KK343 pKa = 10.08 GTTQPFSDD351 pKa = 3.55 TDD353 pKa = 3.57 EE354 pKa = 4.59 FDD356 pKa = 4.19 SNVIIANAGMAAAFMGGGLLSGLGQGIGAYY386 pKa = 9.8 AQGKK390 pKa = 7.52 MQMQMQDD397 pKa = 3.29 KK398 pKa = 8.9 TLSWYY403 pKa = 8.89 EE404 pKa = 4.02 KK405 pKa = 10.65 KK406 pKa = 10.5 QMHH409 pKa = 7.31 DD410 pKa = 3.03 IDD412 pKa = 3.78 NQRR415 pKa = 11.84 YY416 pKa = 7.95 LQQSMFDD423 pKa = 3.98 WKK425 pKa = 11.01 SHH427 pKa = 3.92 MQNRR431 pKa = 11.84 EE432 pKa = 3.14 FDD434 pKa = 3.83 YY435 pKa = 11.43 GNEE438 pKa = 3.9 QQRR441 pKa = 11.84 RR442 pKa = 11.84 QIDD445 pKa = 3.55 WQRR448 pKa = 11.84 SSQAALFSQQNLYY461 pKa = 11.17 QNTQNKK467 pKa = 6.16 FTKK470 pKa = 10.3 EE471 pKa = 3.77 LVEE474 pKa = 4.37 TNLDD478 pKa = 3.19 SDD480 pKa = 3.48 IRR482 pKa = 11.84 RR483 pKa = 11.84 ARR485 pKa = 11.84 NAQQLSGYY493 pKa = 8.32 RR494 pKa = 11.84 TDD496 pKa = 3.47 ATVSGLTNSGRR507 pKa = 11.84 GGLGHH512 pKa = 7.62 PDD514 pKa = 3.38 APRR517 pKa = 11.84 GAILPPRR524 pKa = 11.84 SASTQTNSPKK534 pKa = 10.42 VYY536 pKa = 6.66 TTRR539 pKa = 11.84 PTGFYY544 pKa = 9.02 TGPMSSNDD552 pKa = 3.54 DD553 pKa = 3.99 KK554 pKa = 11.54 II555 pKa = 4.54
Molecular weight: 63.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.062
IPC2_protein 6.109
IPC_protein 6.211
Toseland 6.532
ProMoST 6.825
Dawson 6.517
Bjellqvist 6.517
Wikipedia 6.532
Rodwell 6.504
Grimsley 6.664
Solomon 6.517
Lehninger 6.517
Nozaki 6.766
DTASelect 6.956
Thurlkill 6.971
EMBOSS 6.956
Sillero 6.883
Patrickios 3.961
IPC_peptide 6.532
IPC2_peptide 6.693
IPC2.peptide.svr19 6.747
Protein with the highest isoelectric point:
>tr|O55320|O55320_9VIRU Protein P2 (Fragment) OS=Acyrthosiphon pisum virus OX=340187 PE=4 SV=1
MM1 pKa = 7.29 SLSKK5 pKa = 10.45 PSEE8 pKa = 4.12 LEE10 pKa = 3.68 QVKK13 pKa = 10.12 PSSLWITTYY22 pKa = 8.95 HH23 pKa = 6.85 TIKK26 pKa = 10.61 QLIKK30 pKa = 9.54 EE31 pKa = 4.36 WFTKK35 pKa = 10.43 LVEE38 pKa = 4.25 AVLNPGEE45 pKa = 3.88 IRR47 pKa = 11.84 IAITAVVHH55 pKa = 5.65 TLTGVVLSTMTLFKK69 pKa = 10.81 KK70 pKa = 9.45 GTGYY74 pKa = 11.1 LMKK77 pKa = 10.17 IISGVNLLSTFEE89 pKa = 4.39 LLLVQIGRR97 pKa = 11.84 NKK99 pKa = 10.53 EE100 pKa = 3.92 LFTPDD105 pKa = 2.52 SAMRR109 pKa = 11.84 HH110 pKa = 5.48 AEE112 pKa = 4.27 SKK114 pKa = 10.28 LSEE117 pKa = 4.24 YY118 pKa = 10.6 LDD120 pKa = 4.03 HH121 pKa = 7.39 SDD123 pKa = 4.72 SEE125 pKa = 4.39 EE126 pKa = 4.17 VYY128 pKa = 11.31 ANTEE132 pKa = 3.86 QEE134 pKa = 4.88 AEE136 pKa = 4.32 NIWVGCLSTILTTLFGALLAGFGLSTKK163 pKa = 10.17 GLEE166 pKa = 4.32 GLTTTFRR173 pKa = 11.84 AATTTKK179 pKa = 10.81 NGVFCIKK186 pKa = 10.22 EE187 pKa = 4.06 LVDD190 pKa = 3.5 AALKK194 pKa = 10.41 IIAEE198 pKa = 4.29 EE199 pKa = 4.31 KK200 pKa = 10.03 FDD202 pKa = 3.9 PVYY205 pKa = 10.83 KK206 pKa = 9.91 EE207 pKa = 4.03 RR208 pKa = 11.84 KK209 pKa = 9.56 AITALTEE216 pKa = 3.79 EE217 pKa = 4.35 AQVLLRR223 pKa = 11.84 LNLGAIASDD232 pKa = 3.31 NKK234 pKa = 10.37 VKK236 pKa = 10.66 DD237 pKa = 3.67 RR238 pKa = 11.84 YY239 pKa = 9.14 RR240 pKa = 11.84 TIIKK244 pKa = 9.91 DD245 pKa = 3.11 INQALTTYY253 pKa = 10.02 RR254 pKa = 11.84 HH255 pKa = 5.59 QDD257 pKa = 3.28 SANMRR262 pKa = 11.84 SLLATMVGEE271 pKa = 4.25 LNKK274 pKa = 10.51 KK275 pKa = 8.92 LAEE278 pKa = 4.11 VEE280 pKa = 3.95 ALIPTRR286 pKa = 11.84 QVTAGVYY293 pKa = 9.46 LQGEE297 pKa = 4.43 KK298 pKa = 10.93 GVGKK302 pKa = 10.63 SSLCNYY308 pKa = 8.08 LASYY312 pKa = 9.36 LAEE315 pKa = 4.16 VFDD318 pKa = 4.88 WSPSIYY324 pKa = 10.44 NISPGVKK331 pKa = 9.77 FYY333 pKa = 11.28 EE334 pKa = 4.92 PYY336 pKa = 10.66 LNEE339 pKa = 4.78 DD340 pKa = 3.43 FGILNEE346 pKa = 4.45 FGSTVDD352 pKa = 3.73 RR353 pKa = 11.84 DD354 pKa = 4.21 TFSVDD359 pKa = 2.87 ANRR362 pKa = 11.84 ICSSDD367 pKa = 3.25 PFNFEE372 pKa = 4.0 SAGLAGKK379 pKa = 10.69 VSMCQLKK386 pKa = 10.77 ALFVTTNVLNPDD398 pKa = 3.26 IKK400 pKa = 10.56 EE401 pKa = 4.08 LQPNAVPAFWDD412 pKa = 3.29 RR413 pKa = 11.84 FIIIKK418 pKa = 10.51 VMDD421 pKa = 4.43 PLITDD426 pKa = 3.11 RR427 pKa = 11.84 RR428 pKa = 11.84 KK429 pKa = 9.86 QVNHH433 pKa = 6.64 RR434 pKa = 11.84 KK435 pKa = 9.67 SDD437 pKa = 3.72 FSHH440 pKa = 6.87 LSFRR444 pKa = 11.84 VYY446 pKa = 11.22 DD447 pKa = 4.39 LGTDD451 pKa = 3.56 EE452 pKa = 5.61 NDD454 pKa = 3.24 LPKK457 pKa = 10.44 FRR459 pKa = 11.84 LLYY462 pKa = 10.51 DD463 pKa = 3.75 KK464 pKa = 11.25 LHH466 pKa = 6.85 ISDD469 pKa = 3.99 ILQILRR475 pKa = 11.84 TKK477 pKa = 10.61 LALEE481 pKa = 4.09 TKK483 pKa = 10.46 AFYY486 pKa = 10.93 NRR488 pKa = 11.84 LNYY491 pKa = 9.52 PMPEE495 pKa = 3.93 ALQKK499 pKa = 10.71 IIDD502 pKa = 4.4 LEE504 pKa = 4.73 SNPPPGSYY512 pKa = 10.19 AFKK515 pKa = 10.89 YY516 pKa = 10.56 FGFQEE521 pKa = 4.44 KK522 pKa = 9.82 IVEE525 pKa = 4.16 NSGGRR530 pKa = 11.84 DD531 pKa = 3.48 FCILRR536 pKa = 11.84 LQGPEE541 pKa = 4.02 GTGKK545 pKa = 7.28 TTLAMKK551 pKa = 10.25 LAEE554 pKa = 3.96 RR555 pKa = 11.84 WMEE558 pKa = 3.75 LTTAFEE564 pKa = 4.52 GKK566 pKa = 9.47 SRR568 pKa = 11.84 YY569 pKa = 7.99 TIQKK573 pKa = 9.53 SSSLEE578 pKa = 3.69 EE579 pKa = 3.95 FVPLNTPTIYY589 pKa = 11.11 VLDD592 pKa = 4.02 DD593 pKa = 3.4 WVTSNITIQEE603 pKa = 3.8 SSIYY607 pKa = 7.66 VTKK610 pKa = 10.79 INATTNDD617 pKa = 3.95 SIFIITTNEE626 pKa = 3.61 IIKK629 pKa = 10.41 RR630 pKa = 11.84 RR631 pKa = 11.84 LPWCEE636 pKa = 3.63 LPNRR640 pKa = 11.84 LLVGLLGGTYY650 pKa = 10.13 NYY652 pKa = 9.5 PYY654 pKa = 11.29 DD655 pKa = 3.68 MNPYY659 pKa = 9.12 KK660 pKa = 10.3 EE661 pKa = 5.16 LYY663 pKa = 9.17 SGMRR667 pKa = 11.84 RR668 pKa = 11.84 RR669 pKa = 11.84 IGLDD673 pKa = 2.83 DD674 pKa = 4.85 LYY676 pKa = 11.13 SVRR679 pKa = 11.84 GRR681 pKa = 11.84 VHH683 pKa = 6.94 DD684 pKa = 4.03 VSSTEE689 pKa = 3.6 YY690 pKa = 10.91 CMVVTTDD697 pKa = 3.26 EE698 pKa = 4.8 KK699 pKa = 11.42 SVLTCSGKK707 pKa = 9.76 NVGFPFIVEE716 pKa = 4.32 SIKK719 pKa = 10.59 QRR721 pKa = 11.84 FINMMEE727 pKa = 3.51 NRR729 pKa = 11.84 YY730 pKa = 10.42 NITTIQGRR738 pKa = 11.84 PEE740 pKa = 4.06 NEE742 pKa = 3.86 LPTVDD747 pKa = 6.06 FEE749 pKa = 5.01 IKK751 pKa = 10.11 VANKK755 pKa = 10.32 DD756 pKa = 3.29 ILFKK760 pKa = 10.99 KK761 pKa = 10.63 LSTSLGAASLYY772 pKa = 10.19 LKK774 pKa = 10.28 PDD776 pKa = 3.58 AEE778 pKa = 4.27 ASLYY782 pKa = 9.78 VSEE785 pKa = 5.53 RR786 pKa = 11.84 IISGIAEE793 pKa = 4.26 VNPDD797 pKa = 3.14 KK798 pKa = 10.96 AIRR801 pKa = 11.84 PSDD804 pKa = 4.03 FICKK808 pKa = 9.47 EE809 pKa = 4.01 VFDD812 pKa = 5.57 DD813 pKa = 6.22 SIPTDD818 pKa = 3.31 EE819 pKa = 5.2 RR820 pKa = 11.84 YY821 pKa = 6.05 TTIVEE826 pKa = 4.65 RR827 pKa = 11.84 ITRR830 pKa = 11.84 VLLQYY835 pKa = 10.87 FKK837 pKa = 11.19 DD838 pKa = 3.3 PTFRR842 pKa = 11.84 VVIQDD847 pKa = 3.51 EE848 pKa = 4.34 KK849 pKa = 10.69 RR850 pKa = 11.84 VVYY853 pKa = 10.59 SEE855 pKa = 4.18 NGVVYY860 pKa = 10.29 YY861 pKa = 10.96 YY862 pKa = 10.58 EE863 pKa = 5.0 DD864 pKa = 4.38 KK865 pKa = 10.91 EE866 pKa = 4.12 DD867 pKa = 3.41 TRR869 pKa = 11.84 IAFEE873 pKa = 4.61 GYY875 pKa = 10.41 NLIINLSTPKK885 pKa = 9.9 MVFPYY890 pKa = 10.52 DD891 pKa = 3.71 FAAALVEE898 pKa = 4.61 DD899 pKa = 3.93 RR900 pKa = 11.84 MEE902 pKa = 4.18 KK903 pKa = 10.59 KK904 pKa = 10.46 FVGTLSNLTAAEE916 pKa = 3.75 YY917 pKa = 9.66 TIIEE921 pKa = 4.48 KK922 pKa = 10.45 YY923 pKa = 8.99 YY924 pKa = 10.52 KK925 pKa = 10.34 RR926 pKa = 11.84 SITSKK931 pKa = 9.66 KK932 pKa = 10.2 CNPFLVAVATRR943 pKa = 11.84 EE944 pKa = 4.07 LQLTRR949 pKa = 11.84 MKK951 pKa = 10.7 DD952 pKa = 3.22 PKK954 pKa = 10.45 YY955 pKa = 11.11 VKK957 pKa = 10.61 GLLKK961 pKa = 10.61 LQNNYY966 pKa = 9.57 LLKK969 pKa = 10.98 LGLCGLAGVGSVALLYY985 pKa = 10.59 GVYY988 pKa = 10.55 KK989 pKa = 10.88 LIASTMKK996 pKa = 10.52 QEE998 pKa = 3.98 EE999 pKa = 4.33 EE1000 pKa = 4.3 KK1001 pKa = 10.93 IKK1003 pKa = 11.2 DD1004 pKa = 3.39 NAFANVGSTPPKK1016 pKa = 9.62 RR1017 pKa = 11.84 AMNNQSNKK1025 pKa = 10.18 DD1026 pKa = 3.64 KK1027 pKa = 10.94 QRR1029 pKa = 11.84 LGATLTDD1036 pKa = 4.8 PIIEE1040 pKa = 4.31 NSPHH1044 pKa = 5.95 HH1045 pKa = 5.99 KK1046 pKa = 9.89 HH1047 pKa = 6.63 RR1048 pKa = 11.84 GRR1050 pKa = 11.84 IIPNKK1055 pKa = 9.82 KK1056 pKa = 8.0 RR1057 pKa = 11.84 TSRR1060 pKa = 11.84 YY1061 pKa = 7.42 ATTSTSSSSEE1071 pKa = 3.95 TTTTSASSSDD1081 pKa = 4.31 EE1082 pKa = 3.84 ITSDD1086 pKa = 3.31 SDD1088 pKa = 3.75 SSISSDD1094 pKa = 2.96 SSVKK1098 pKa = 10.28 PEE1100 pKa = 4.91 PITIVGNPAHH1110 pKa = 7.24 RR1111 pKa = 11.84 IIGNSAHH1118 pKa = 5.87 TKK1120 pKa = 9.81 NRR1122 pKa = 11.84 GTIIPNNHH1130 pKa = 3.89 TRR1132 pKa = 11.84 EE1133 pKa = 3.92 IKK1135 pKa = 10.51 RR1136 pKa = 11.84 VDD1138 pKa = 3.22 RR1139 pKa = 11.84 AKK1141 pKa = 10.86 KK1142 pKa = 10.22 EE1143 pKa = 3.85 NEE1145 pKa = 4.32 DD1146 pKa = 3.81 YY1147 pKa = 11.46 NKK1149 pKa = 9.85 VPHH1152 pKa = 6.05 EE1153 pKa = 4.5 HH1154 pKa = 6.24 VCTTCGCLFTHH1165 pKa = 5.54 IHH1167 pKa = 6.19 KK1168 pKa = 10.43 FNKK1171 pKa = 9.73 NFLHH1175 pKa = 6.15 PTYY1178 pKa = 11.0 QNACPNYY1185 pKa = 9.66 KK1186 pKa = 9.66 CASYY1190 pKa = 10.56 HH1191 pKa = 6.41 GGNNKK1196 pKa = 9.6 YY1197 pKa = 9.21 NAKK1200 pKa = 10.28 LIQADD1205 pKa = 4.88 FINSADD1211 pKa = 3.98 EE1212 pKa = 4.28 PLTPNAMTKK1221 pKa = 10.76 LMGEE1225 pKa = 4.55 EE1226 pKa = 3.97 YY1227 pKa = 11.0 APVKK1231 pKa = 10.77 VSLLEE1236 pKa = 4.02 FFSALPSEE1244 pKa = 4.51 EE1245 pKa = 3.85 RR1246 pKa = 11.84 KK1247 pKa = 9.49 QVYY1250 pKa = 10.12 DD1251 pKa = 4.07 YY1252 pKa = 11.41 INQARR1257 pKa = 11.84 DD1258 pKa = 3.25 ANDD1261 pKa = 3.15 RR1262 pKa = 11.84 TFARR1266 pKa = 11.84 LCRR1269 pKa = 11.84 KK1270 pKa = 9.89 YY1271 pKa = 10.3 PGLEE1275 pKa = 3.76 NCIMKK1280 pKa = 10.3 AVHH1283 pKa = 6.82 ANMLTDD1289 pKa = 4.98 ADD1291 pKa = 4.85 MITNDD1296 pKa = 3.95 DD1297 pKa = 3.57 TDD1299 pKa = 5.38 LEE1301 pKa = 4.21 ILHH1304 pKa = 6.7 KK1305 pKa = 10.13 KK1306 pKa = 9.17 LNKK1309 pKa = 10.26 AYY1311 pKa = 10.6 VQVNYY1316 pKa = 10.93 AGFRR1320 pKa = 11.84 AYY1322 pKa = 10.27 ALHH1325 pKa = 6.55 VGEE1328 pKa = 5.22 GKK1330 pKa = 10.47 FLTVAHH1336 pKa = 6.66 CFDD1339 pKa = 3.63 SVGRR1343 pKa = 11.84 SLVLLSEE1350 pKa = 4.56 GKK1352 pKa = 9.88 SYY1354 pKa = 10.82 PGVVVSITRR1363 pKa = 11.84 EE1364 pKa = 3.55 RR1365 pKa = 11.84 DD1366 pKa = 3.12 LSVVYY1371 pKa = 10.52 CKK1373 pKa = 10.31 EE1374 pKa = 3.76 LSNLRR1379 pKa = 11.84 SAKK1382 pKa = 10.12 SAFVKK1387 pKa = 10.64 DD1388 pKa = 3.56 SSDD1391 pKa = 3.52 LVLGGYY1397 pKa = 8.05 FMRR1400 pKa = 11.84 CGPCVTIVSGNIEE1413 pKa = 3.75 YY1414 pKa = 10.49 RR1415 pKa = 11.84 EE1416 pKa = 3.92 YY1417 pKa = 11.14 QEE1419 pKa = 6.02 FIDD1422 pKa = 4.96 GSCSNEE1428 pKa = 3.8 YY1429 pKa = 9.98 YY1430 pKa = 10.04 RR1431 pKa = 11.84 PSMEE1435 pKa = 3.87 VLKK1438 pKa = 10.6 LRR1440 pKa = 11.84 RR1441 pKa = 11.84 VGLVVTEE1448 pKa = 4.76 TIRR1451 pKa = 11.84 KK1452 pKa = 8.36 GDD1454 pKa = 3.59 CGFPFVARR1462 pKa = 11.84 INGTMRR1468 pKa = 11.84 IIGIHH1473 pKa = 5.55 NAYY1476 pKa = 7.39 KK1477 pKa = 8.63 THH1479 pKa = 5.59 TVVFGAFISQPLIEE1493 pKa = 4.67 EE1494 pKa = 4.44 LCSVVVPNADD1504 pKa = 3.42 VPSTAVVVKK1513 pKa = 10.24 VPVIRR1518 pKa = 11.84 DD1519 pKa = 3.57 DD1520 pKa = 3.85 NYY1522 pKa = 10.99 NDD1524 pKa = 3.7 YY1525 pKa = 11.5 LEE1527 pKa = 4.54 MSMPMEE1533 pKa = 4.05 YY1534 pKa = 10.55 AEE1536 pKa = 4.26 EE1537 pKa = 4.03 FKK1539 pKa = 10.95 KK1540 pKa = 10.87 CFSPDD1545 pKa = 2.62 PYY1547 pKa = 10.16 MPKK1550 pKa = 10.44 NSGLTSIGFNQKK1562 pKa = 8.84 FRR1564 pKa = 11.84 NYY1566 pKa = 8.67 NHH1568 pKa = 6.36 MKK1570 pKa = 9.7 EE1571 pKa = 3.81 KK1572 pKa = 10.7 HH1573 pKa = 4.97 FTHH1576 pKa = 7.02 EE1577 pKa = 4.21 LTLEE1581 pKa = 4.18 NANTCLPAAFSDD1593 pKa = 4.19 RR1594 pKa = 11.84 YY1595 pKa = 9.31 VTDD1598 pKa = 3.03 TSEE1601 pKa = 3.92 LVKK1604 pKa = 11.08 NGFGKK1609 pKa = 9.69 PDD1611 pKa = 4.12 PLWTQACKK1619 pKa = 10.32 YY1620 pKa = 10.0 GRR1622 pKa = 11.84 RR1623 pKa = 11.84 NKK1625 pKa = 10.65 NYY1627 pKa = 8.61 DD1628 pKa = 2.88 RR1629 pKa = 11.84 HH1630 pKa = 6.19 IFRR1633 pKa = 11.84 HH1634 pKa = 4.94 AVEE1637 pKa = 4.28 MALDD1641 pKa = 4.07 YY1642 pKa = 11.89 NKK1644 pKa = 8.32 MTYY1647 pKa = 10.14 CAEE1650 pKa = 4.19 KK1651 pKa = 10.0 PFKK1654 pKa = 10.57 FLTEE1658 pKa = 4.21 FEE1660 pKa = 4.36 ALNGRR1665 pKa = 11.84 TEE1667 pKa = 4.14 PFLSNVDD1674 pKa = 3.19 ITTSAGPYY1682 pKa = 9.86 AKK1684 pKa = 9.87 YY1685 pKa = 10.08 FHH1687 pKa = 7.04 NIYY1690 pKa = 9.44 TKK1692 pKa = 10.55 RR1693 pKa = 11.84 DD1694 pKa = 3.41 ILDD1697 pKa = 3.49 TEE1699 pKa = 4.4 LADD1702 pKa = 4.27 GKK1704 pKa = 9.81 PIYY1707 pKa = 10.55 KK1708 pKa = 10.17 FAQNRR1713 pKa = 11.84 AGQSIRR1719 pKa = 11.84 NHH1721 pKa = 6.03 LKK1723 pKa = 9.57 SQRR1726 pKa = 11.84 NLLAVYY1732 pKa = 7.93 GIPPCLISQDD1742 pKa = 3.48 NAKK1745 pKa = 10.22 VEE1747 pKa = 4.36 NIEE1750 pKa = 4.49 KK1751 pKa = 10.61 EE1752 pKa = 4.05 KK1753 pKa = 10.41 AQKK1756 pKa = 10.86 GKK1758 pKa = 10.25 VRR1760 pKa = 11.84 LFNNVDD1766 pKa = 3.53 PAVNAILKK1774 pKa = 10.37 IMFGDD1779 pKa = 3.7 WFSRR1783 pKa = 11.84 AMAKK1787 pKa = 10.24 SSEE1790 pKa = 4.12 GYY1792 pKa = 9.71 YY1793 pKa = 10.61 AIGQNPYY1800 pKa = 7.74 TTSTEE1805 pKa = 3.13 IWHH1808 pKa = 6.62 RR1809 pKa = 11.84 FSTKK1813 pKa = 8.94 QGKK1816 pKa = 8.54 ILNTDD1821 pKa = 4.17 FKK1823 pKa = 11.53 AFDD1826 pKa = 3.62 KK1827 pKa = 11.34 LLITEE1832 pKa = 5.45 LIEE1835 pKa = 3.94 AFCYY1839 pKa = 10.15 IGGEE1843 pKa = 3.94 LTKK1846 pKa = 10.67 NEE1848 pKa = 4.3 KK1849 pKa = 10.25 HH1850 pKa = 6.03 PQSALMYY1857 pKa = 8.73 EE1858 pKa = 5.01 AISLTLIHH1866 pKa = 6.84 AVHH1869 pKa = 6.99 ILNGSVYY1876 pKa = 10.46 VVNNGNEE1883 pKa = 3.97 SGTFVTTLLNCVSVHH1898 pKa = 6.97 IIFNYY1903 pKa = 10.26 SFIVCWNKK1911 pKa = 9.37 VPSYY1915 pKa = 10.81 VHH1917 pKa = 6.77 IKK1919 pKa = 10.19 PLLKK1923 pKa = 10.48 DD1924 pKa = 3.01 IMSRR1928 pKa = 11.84 SEE1930 pKa = 3.81 LAILGDD1936 pKa = 4.3 DD1937 pKa = 3.49 KK1938 pKa = 11.33 TQVVSKK1944 pKa = 9.51 DD1945 pKa = 3.01 IPMEE1949 pKa = 4.21 EE1950 pKa = 4.59 EE1951 pKa = 3.96 DD1952 pKa = 5.38 LIDD1955 pKa = 3.51 IAASLGMEE1963 pKa = 4.17 CTKK1966 pKa = 11.1 AKK1968 pKa = 10.66 GGLDD1972 pKa = 3.48 DD1973 pKa = 4.83 GKK1975 pKa = 10.87 QINFCSRR1982 pKa = 11.84 VLVWDD1987 pKa = 3.61 EE1988 pKa = 4.23 VEE1990 pKa = 4.16 QIVYY1994 pKa = 9.51 PRR1996 pKa = 11.84 LKK1998 pKa = 9.8 KK1999 pKa = 10.65 SSIIGLLYY2007 pKa = 10.16 WFASLDD2013 pKa = 3.65 KK2014 pKa = 11.3 NQVRR2018 pKa = 11.84 DD2019 pKa = 3.69 NLMIALFEE2027 pKa = 4.59 ASLHH2031 pKa = 5.04 EE2032 pKa = 4.15 RR2033 pKa = 11.84 EE2034 pKa = 5.01 FYY2036 pKa = 11.11 DD2037 pKa = 4.22 SVLRR2041 pKa = 11.84 DD2042 pKa = 3.37 AMLVSLEE2049 pKa = 4.09 FGVDD2053 pKa = 2.71 IRR2055 pKa = 11.84 TVAFTNYY2062 pKa = 8.92 SQSRR2066 pKa = 11.84 QRR2068 pKa = 11.84 LKK2070 pKa = 11.4 SMLMNDD2076 pKa = 4.44 FEE2078 pKa = 4.49 YY2079 pKa = 10.97 QQISALADD2087 pKa = 3.66 RR2088 pKa = 11.84 KK2089 pKa = 8.61 TKK2091 pKa = 10.55 EE2092 pKa = 3.71 SDD2094 pKa = 3.33 YY2095 pKa = 11.33 SDD2097 pKa = 5.45 LIRR2100 pKa = 11.84 ILKK2103 pKa = 7.6 TAQLLEE2109 pKa = 3.96 NDD2111 pKa = 3.19 IQKK2114 pKa = 11.16 AKK2116 pKa = 9.49 TADD2119 pKa = 3.21 INNFSVVKK2127 pKa = 10.41 NRR2129 pKa = 11.84 ALAIQKK2135 pKa = 7.69 YY2136 pKa = 5.19 TTTSTTPTTGSVVYY2150 pKa = 9.27 EE2151 pKa = 4.23 SEE2153 pKa = 4.16 MAPLSTNSNPISACLEE2169 pKa = 4.62 LISKK2173 pKa = 10.01 LKK2175 pKa = 8.67 TAGPVEE2181 pKa = 4.18 HH2182 pKa = 7.02 FEE2184 pKa = 4.39 RR2185 pKa = 11.84 AGPPHH2190 pKa = 7.1 SPTYY2194 pKa = 10.57 SCTVTYY2200 pKa = 10.52 AGRR2203 pKa = 11.84 HH2204 pKa = 4.89 FGGEE2208 pKa = 4.48 GPSKK2212 pKa = 10.91 ASAKK2216 pKa = 7.5 TQAYY2220 pKa = 7.94 GALRR2224 pKa = 11.84 QYY2226 pKa = 11.3 LEE2228 pKa = 3.94 DD2229 pKa = 5.39 HH2230 pKa = 6.46 IVCNAACEE2238 pKa = 4.09 DD2239 pKa = 3.63 QRR2241 pKa = 11.84 IEE2243 pKa = 3.89 ATQKK2247 pKa = 8.99 IRR2249 pKa = 11.84 KK2250 pKa = 7.95 VAITAFSFYY2259 pKa = 10.38 IEE2261 pKa = 4.06 NHH2263 pKa = 5.85 INTAINNADD2272 pKa = 3.31 GKK2274 pKa = 10.79 KK2275 pKa = 10.05 YY2276 pKa = 10.39 IIIIGKK2282 pKa = 9.82 RR2283 pKa = 11.84 SADD2286 pKa = 3.51 DD2287 pKa = 3.38 TVEE2290 pKa = 4.22 QIDD2293 pKa = 4.05 GFYY2296 pKa = 10.58 KK2297 pKa = 9.49 FHH2299 pKa = 7.61 RR2300 pKa = 11.84 GGNCYY2305 pKa = 9.69 ILSPIIEE2312 pKa = 4.14 KK2313 pKa = 10.56 LGRR2316 pKa = 11.84 KK2317 pKa = 6.57 TAAEE2321 pKa = 4.57 CYY2323 pKa = 9.22 RR2324 pKa = 11.84 YY2325 pKa = 10.26 YY2326 pKa = 10.37 PGVSFDD2332 pKa = 3.39 SSTNEE2337 pKa = 3.53 VTLDD2341 pKa = 3.33 VHH2343 pKa = 7.15 ASTSNYY2349 pKa = 9.3 SLRR2352 pKa = 11.84 DD2353 pKa = 3.86 DD2354 pKa = 4.17 FEE2356 pKa = 6.65 KK2357 pKa = 10.66 DD2358 pKa = 3.48 YY2359 pKa = 11.44 TDD2361 pKa = 5.05 DD2362 pKa = 3.65 DD2363 pKa = 4.37 HH2364 pKa = 9.07 LVFANSAEE2372 pKa = 4.21 DD2373 pKa = 3.93 KK2374 pKa = 10.58 PILGVMEE2381 pKa = 4.89 NDD2383 pKa = 4.68 DD2384 pKa = 4.46 PQTMPVTSVMDD2395 pKa = 3.69 SDD2397 pKa = 3.73 KK2398 pKa = 11.45 ALYY2401 pKa = 9.79 QPIIMNRR2408 pKa = 11.84 SAPNNSWLRR2417 pKa = 11.84 AGGITFNIHH2426 pKa = 6.52 DD2427 pKa = 4.19 LVYY2430 pKa = 10.87 NQFVGCNKK2438 pKa = 10.09 QITVGDD2444 pKa = 3.8 GTAAGTILAQIPYY2457 pKa = 10.28 DD2458 pKa = 3.84 PVDD2461 pKa = 3.24 NPYY2464 pKa = 11.55 ANGYY2468 pKa = 7.89 IQNLVLLHH2476 pKa = 6.3 GRR2478 pKa = 11.84 MTGDD2482 pKa = 2.65 WMIKK2486 pKa = 7.9 ITCPGNPGMQCSIGVGWSPTKK2507 pKa = 10.29 ISEE2510 pKa = 4.39 TTVPMDD2516 pKa = 4.66 VITKK2520 pKa = 9.65 YY2521 pKa = 11.14 AFFTTGVSSEE2531 pKa = 3.84 WSHH2534 pKa = 6.18 TIVMTDD2540 pKa = 2.65 ARR2542 pKa = 11.84 QNNFYY2547 pKa = 9.95 RR2548 pKa = 11.84 TIQRR2552 pKa = 11.84 DD2553 pKa = 3.7 SKK2555 pKa = 9.47 KK2556 pKa = 8.58 TQVDD2560 pKa = 3.6 DD2561 pKa = 4.01 VGNMPHH2567 pKa = 7.48 IVIFCEE2573 pKa = 4.1 TPPTSVYY2580 pKa = 8.9 TEE2582 pKa = 4.01 KK2583 pKa = 10.84 KK2584 pKa = 8.38 STYY2587 pKa = 8.91 LTFFSKK2593 pKa = 10.71 LCSEE2597 pKa = 4.56 NDD2599 pKa = 3.28 LMFNPSIKK2607 pKa = 10.23 PFVLADD2613 pKa = 3.5 PQQSVKK2619 pKa = 10.23 YY2620 pKa = 9.09 DD2621 pKa = 3.23 ARR2623 pKa = 11.84 KK2624 pKa = 10.08 GYY2626 pKa = 10.38 SLNFF2630 pKa = 3.63
Molecular weight: 296.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.417
IPC2_protein 6.59
IPC_protein 6.561
Toseland 6.62
ProMoST 7.044
Dawson 7.044
Bjellqvist 7.161
Wikipedia 7.029
Rodwell 7.059
Grimsley 6.722
Solomon 7.059
Lehninger 7.059
Nozaki 7.424
DTASelect 7.395
Thurlkill 7.424
EMBOSS 7.41
Sillero 7.497
Patrickios 4.101
IPC_peptide 7.059
IPC2_peptide 6.942
IPC2.peptide.svr19 6.867
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3185
555
2630
1592.5
179.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.531 ± 0.148
1.601 ± 0.412
5.557 ± 0.013
5.871 ± 0.639
4.678 ± 0.761
5.369 ± 0.101
1.915 ± 0.221
6.876 ± 0.098
6.688 ± 0.768
8.352 ± 0.114
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.166 ± 0.086
5.934 ± 0.174
4.239 ± 0.044
3.548 ± 1.795
4.615 ± 0.032
7.41 ± 0.242
7.127 ± 0.131
5.997 ± 1.119
0.722 ± 0.084
4.804 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here