Torque teno Leptonychotes weddellii virus-1
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RVW0|A0A1Z2RVW0_9VIRU ORF2 OS=Torque teno Leptonychotes weddellii virus-1 OX=2012676 PE=4 SV=1
MM1 pKa = 7.24 ISYY4 pKa = 10.5 RR5 pKa = 11.84 EE6 pKa = 3.93 TSTQTEE12 pKa = 4.5 CSRR15 pKa = 11.84 KK16 pKa = 9.19 EE17 pKa = 3.85 LMRR20 pKa = 11.84 EE21 pKa = 3.6 LLEE24 pKa = 5.55 IIDD27 pKa = 3.93 VTSQPEE33 pKa = 3.94 WGMSPIGSPPEE44 pKa = 4.15 SEE46 pKa = 4.35 SDD48 pKa = 3.3 SDD50 pKa = 4.4 SLIEE54 pKa = 4.12 NSSTGSGSSSEE65 pKa = 4.96 FYY67 pKa = 10.46 WEE69 pKa = 5.41 DD70 pKa = 3.13 EE71 pKa = 4.4 TNPGGEE77 pKa = 4.56 GPPLVPPQQ85 pKa = 3.45
Molecular weight: 9.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.206
IPC2_protein 3.91
IPC_protein 3.757
Toseland 3.605
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.872
Wikipedia 3.592
Rodwell 3.605
Grimsley 3.528
Solomon 3.681
Lehninger 3.643
Nozaki 3.846
DTASelect 3.91
Thurlkill 3.643
EMBOSS 3.605
Sillero 3.872
Patrickios 3.3
IPC_peptide 3.694
IPC2_peptide 3.859
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A1Z2RVY6|A0A1Z2RVY6_9VIRU Capsid protein OS=Torque teno Leptonychotes weddellii virus-1 OX=2012676 PE=3 SV=1
MM1 pKa = 7.84 AYY3 pKa = 10.03 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 WRR9 pKa = 11.84 KK10 pKa = 7.57 PRR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 WRR17 pKa = 11.84 TYY19 pKa = 9.7 RR20 pKa = 11.84 RR21 pKa = 11.84 NRR23 pKa = 11.84 YY24 pKa = 5.39 WRR26 pKa = 11.84 RR27 pKa = 11.84 PHH29 pKa = 5.97 RR30 pKa = 11.84 RR31 pKa = 11.84 HH32 pKa = 4.26 WRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 YY38 pKa = 9.27 IRR40 pKa = 11.84 PRR42 pKa = 11.84 TATVRR47 pKa = 11.84 YY48 pKa = 8.07 YY49 pKa = 10.09 PSRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 KK55 pKa = 9.59 RR56 pKa = 11.84 ISVRR60 pKa = 11.84 GWEE63 pKa = 4.02 PLGNVCSSDD72 pKa = 3.19 IASSEE77 pKa = 3.88 ATPYY81 pKa = 10.53 TDD83 pKa = 5.02 LDD85 pKa = 3.97 MYY87 pKa = 11.17 DD88 pKa = 4.73 LSPLEE93 pKa = 4.78 DD94 pKa = 3.42 DD95 pKa = 3.27 TRR97 pKa = 11.84 KK98 pKa = 10.65 YY99 pKa = 9.38 KK100 pKa = 10.89 GKK102 pKa = 8.35 WHH104 pKa = 5.96 GTWGHH109 pKa = 6.25 HH110 pKa = 5.49 FFTLRR115 pKa = 11.84 SLLVRR120 pKa = 11.84 AKK122 pKa = 10.79 YY123 pKa = 9.84 YY124 pKa = 10.56 FNYY127 pKa = 9.34 WSSDD131 pKa = 2.91 WEE133 pKa = 4.56 GYY135 pKa = 10.88 DD136 pKa = 3.4 YY137 pKa = 11.83 VKK139 pKa = 10.96 FKK141 pKa = 10.99 GGYY144 pKa = 7.56 IWIPRR149 pKa = 11.84 MTYY152 pKa = 10.13 FSWIFYY158 pKa = 9.97 LDD160 pKa = 3.53 SSIQSNPRR168 pKa = 11.84 EE169 pKa = 4.34 DD170 pKa = 4.3 GEE172 pKa = 4.33 PEE174 pKa = 3.7 AKK176 pKa = 10.05 YY177 pKa = 10.28 KK178 pKa = 10.55 YY179 pKa = 8.95 EE180 pKa = 4.28 KK181 pKa = 10.3 SWLHH185 pKa = 6.31 PGILLNRR192 pKa = 11.84 PGSKK196 pKa = 10.56 LMISSLQQPNRR207 pKa = 11.84 PFYY210 pKa = 10.8 RR211 pKa = 11.84 RR212 pKa = 11.84 IKK214 pKa = 8.54 VTPPSAWEE222 pKa = 3.75 GNYY225 pKa = 10.57 RR226 pKa = 11.84 MDD228 pKa = 3.3 TAMDD232 pKa = 3.61 YY233 pKa = 11.58 LLFHH237 pKa = 7.05 WGWTVCNVTASFFDD251 pKa = 4.35 FFCQRR256 pKa = 11.84 KK257 pKa = 9.28 RR258 pKa = 11.84 SATHH262 pKa = 6.76 NDD264 pKa = 3.39 TCPTTPWFIHH274 pKa = 6.07 DD275 pKa = 4.27 SCWKK279 pKa = 10.03 QGITANEE286 pKa = 3.73 VCKK289 pKa = 10.45 AIKK292 pKa = 9.6 EE293 pKa = 4.28 VYY295 pKa = 10.02 DD296 pKa = 4.67 RR297 pKa = 11.84 INKK300 pKa = 8.93 RR301 pKa = 11.84 HH302 pKa = 6.56 LDD304 pKa = 3.66 PRR306 pKa = 11.84 SVWVNRR312 pKa = 11.84 KK313 pKa = 8.34 MYY315 pKa = 10.41 IKK317 pKa = 9.84 SDD319 pKa = 3.76 CVNTPNSNSVPSNCHH334 pKa = 3.44 NWGPFLPQNVILEE347 pKa = 4.45 TSTGNSLYY355 pKa = 10.33 FRR357 pKa = 11.84 YY358 pKa = 10.29 KK359 pKa = 10.73 LFFEE363 pKa = 4.55 VSGDD367 pKa = 3.24 SLYY370 pKa = 10.72 RR371 pKa = 11.84 RR372 pKa = 11.84 QPSQPCKK379 pKa = 10.74 DD380 pKa = 3.58 GVIPPAPGAEE390 pKa = 4.33 SACPEE395 pKa = 3.47 ISTRR399 pKa = 11.84 SIYY402 pKa = 10.17 KK403 pKa = 9.77 KK404 pKa = 10.14 KK405 pKa = 10.3 KK406 pKa = 8.92 RR407 pKa = 11.84 PQSIYY412 pKa = 10.75 DD413 pKa = 3.62 ILQGDD418 pKa = 4.61 LDD420 pKa = 4.41 SDD422 pKa = 3.81 GMLTEE427 pKa = 4.41 RR428 pKa = 11.84 AYY430 pKa = 10.5 EE431 pKa = 4.52 RR432 pKa = 11.84 ITGDD436 pKa = 3.45 NRR438 pKa = 11.84 CDD440 pKa = 3.23 QPTRR444 pKa = 11.84 MGDD447 pKa = 3.35 VPHH450 pKa = 7.05 RR451 pKa = 11.84 VPPRR455 pKa = 11.84 KK456 pKa = 9.1 RR457 pKa = 11.84 VRR459 pKa = 11.84 FRR461 pKa = 11.84 LSDD464 pKa = 3.28 RR465 pKa = 11.84 KK466 pKa = 9.39 LQHH469 pKa = 6.8 RR470 pKa = 11.84 KK471 pKa = 9.72 RR472 pKa = 11.84 EE473 pKa = 4.28 LIRR476 pKa = 11.84 ILLGRR481 pKa = 11.84 RR482 pKa = 11.84 DD483 pKa = 3.57 QPRR486 pKa = 11.84 GGGSPPSPPPVEE498 pKa = 4.35 EE499 pKa = 4.6 PLDD502 pKa = 3.86 LLLNFPKK509 pKa = 10.71
Molecular weight: 60.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.589
IPC_protein 10.087
Toseland 10.116
ProMoST 9.926
Dawson 10.35
Bjellqvist 10.087
Wikipedia 10.57
Rodwell 10.584
Grimsley 10.438
Solomon 10.379
Lehninger 10.335
Nozaki 10.145
DTASelect 10.072
Thurlkill 10.189
EMBOSS 10.526
Sillero 10.277
Patrickios 9.838
IPC_peptide 10.379
IPC2_peptide 9.077
IPC2.peptide.svr19 8.594
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
767
80
509
191.8
22.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.738 ± 0.961
2.347 ± 0.44
5.737 ± 0.572
5.606 ± 2.174
3.781 ± 0.843
7.301 ± 2.728
2.608 ± 0.642
5.215 ± 0.845
4.954 ± 1.079
7.823 ± 2.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.956 ± 0.382
3.651 ± 0.533
7.953 ± 0.876
2.999 ± 0.635
9.778 ± 3.304
8.735 ± 2.099
4.694 ± 0.824
4.042 ± 1.086
3.259 ± 0.525
4.824 ± 1.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here