Streptomyces inhibens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371Q4E7|A0A371Q4E7_9ACTN Uncharacterized protein OS=Streptomyces inhibens OX=2293571 GN=DY245_15015 PE=4 SV=1
MM1 pKa = 7.29PRR3 pKa = 11.84EE4 pKa = 4.25GGVPDD9 pKa = 4.03GTGQPLGATGYY20 pKa = 9.96GYY22 pKa = 11.12VRR24 pKa = 11.84VGPTILPVWLLWLMAALLAVGGVTAYY50 pKa = 10.97VLLNSSPEE58 pKa = 3.99PSQQAVDD65 pKa = 3.98VVPSTVPPPSPDD77 pKa = 2.92VDD79 pKa = 4.3FVPPADD85 pKa = 4.14GDD87 pKa = 4.13SPSASDD93 pKa = 4.19ISPSPDD99 pKa = 3.15DD100 pKa = 3.73ASAIVEE106 pKa = 4.4EE107 pKa = 5.37FYY109 pKa = 11.33QDD111 pKa = 4.12INDD114 pKa = 4.14HH115 pKa = 6.72DD116 pKa = 5.07FAAAWDD122 pKa = 4.16LGGKK126 pKa = 9.7NIGGSSYY133 pKa = 10.71SEE135 pKa = 3.85WVAGYY140 pKa = 7.57DD141 pKa = 3.39TTASIEE147 pKa = 3.95LSAVNTEE154 pKa = 4.19SPGQVSADD162 pKa = 3.4LRR164 pKa = 11.84ATQSDD169 pKa = 3.93GSVRR173 pKa = 11.84VYY175 pKa = 10.56QGTYY179 pKa = 7.74TVSDD183 pKa = 4.07GVIVGADD190 pKa = 3.12ITLKK194 pKa = 10.92

Molecular weight: 20.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371Q0H6|A0A371Q0H6_9ACTN TIGR02234 family membrane protein OS=Streptomyces inhibens OX=2293571 GN=DY245_22885 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7955

0

7955

2512952

26

3663

315.9

33.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.773 ± 0.038

0.841 ± 0.009

5.861 ± 0.02

5.538 ± 0.026

2.726 ± 0.014

9.449 ± 0.024

2.43 ± 0.014

3.338 ± 0.017

2.228 ± 0.024

10.411 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.773 ± 0.011

1.764 ± 0.015

6.227 ± 0.021

2.91 ± 0.017

8.058 ± 0.031

5.02 ± 0.019

6.08 ± 0.025

8.014 ± 0.026

1.511 ± 0.012

2.046 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski