Pedobacter sp. AR-2-6

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3664 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U1C8R5|A0A4U1C8R5_9SPHI Glycoside hydrolase family 25 protein OS=Pedobacter sp. AR-2-6 OX=2571270 GN=FA045_11005 PE=3 SV=1
MM1 pKa = 7.67SDD3 pKa = 2.87INIVVEE9 pKa = 4.31DD10 pKa = 3.7RR11 pKa = 11.84AGNTSEE17 pKa = 5.33LVAPTDD23 pKa = 3.37MGLSLMEE30 pKa = 3.89FLKK33 pKa = 10.88ACEE36 pKa = 3.91YY37 pKa = 11.14DD38 pKa = 3.76VLATCGGMALCATCCVDD55 pKa = 3.88VMEE58 pKa = 5.31GEE60 pKa = 4.41EE61 pKa = 4.04NLKK64 pKa = 10.63EE65 pKa = 4.05MSDD68 pKa = 4.12DD69 pKa = 3.79EE70 pKa = 4.7YY71 pKa = 11.96AMLDD75 pKa = 3.8TLPDD79 pKa = 4.06LLPNSRR85 pKa = 11.84LACQLQLSPAMNGLKK100 pKa = 10.2VRR102 pKa = 11.84LHH104 pKa = 7.21AMDD107 pKa = 3.76

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V5NY86|A0A4V5NY86_9SPHI Uncharacterized protein OS=Pedobacter sp. AR-2-6 OX=2571270 GN=FA045_05010 PE=4 SV=1
MM1 pKa = 7.78GLRR4 pKa = 11.84KK5 pKa = 9.8FKK7 pKa = 10.46PVTPGTRR14 pKa = 11.84FRR16 pKa = 11.84VGASFSEE23 pKa = 4.18ITATKK28 pKa = 9.4PEE30 pKa = 3.92KK31 pKa = 10.78SLVVSSKK38 pKa = 10.94KK39 pKa = 10.5SGGRR43 pKa = 11.84NNTGKK48 pKa = 8.1MTMRR52 pKa = 11.84YY53 pKa = 8.57MGGGHH58 pKa = 6.3KK59 pKa = 10.01KK60 pKa = 9.96SYY62 pKa = 10.67RR63 pKa = 11.84LIDD66 pKa = 3.84FKK68 pKa = 10.93RR69 pKa = 11.84DD70 pKa = 3.21KK71 pKa = 11.05FNIPATVATVEE82 pKa = 3.94YY83 pKa = 10.46DD84 pKa = 3.37PNRR87 pKa = 11.84TARR90 pKa = 11.84IALLHH95 pKa = 5.81YY96 pKa = 10.78ADD98 pKa = 3.89GEE100 pKa = 4.06KK101 pKa = 10.2RR102 pKa = 11.84YY103 pKa = 10.16IIAPEE108 pKa = 4.2GLQVGQTVLSGDD120 pKa = 3.36NATPEE125 pKa = 4.15VGNTLTLANIPLGSIIHH142 pKa = 6.08NLEE145 pKa = 3.78IQPGRR150 pKa = 11.84GAQLARR156 pKa = 11.84SAGAYY161 pKa = 8.78AQLAARR167 pKa = 11.84DD168 pKa = 3.82GRR170 pKa = 11.84YY171 pKa = 8.17ATIKK175 pKa = 9.45MPSGEE180 pKa = 4.09VRR182 pKa = 11.84SILVTCLATIGAVSNADD199 pKa = 3.29HH200 pKa = 6.76ANEE203 pKa = 4.04VLGKK207 pKa = 10.23AGRR210 pKa = 11.84ARR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.2GRR238 pKa = 11.84SSGGQPRR245 pKa = 11.84SRR247 pKa = 11.84NGVYY251 pKa = 10.49SKK253 pKa = 11.1GFKK256 pKa = 9.22TRR258 pKa = 11.84SLKK261 pKa = 10.55KK262 pKa = 8.95YY263 pKa = 7.96SNRR266 pKa = 11.84FIIEE270 pKa = 3.89KK271 pKa = 10.02RR272 pKa = 11.84KK273 pKa = 9.88KK274 pKa = 9.89

Molecular weight: 29.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3664

0

3664

1228412

25

2388

335.3

37.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.38 ± 0.041

0.753 ± 0.012

5.277 ± 0.025

5.903 ± 0.042

5.14 ± 0.032

6.535 ± 0.039

1.658 ± 0.018

7.831 ± 0.044

7.853 ± 0.042

9.544 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.017

6.209 ± 0.032

3.517 ± 0.02

3.646 ± 0.028

3.428 ± 0.025

6.083 ± 0.03

5.781 ± 0.039

6.219 ± 0.025

1.077 ± 0.013

3.938 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski