Amino acid dipepetide frequency for Pedobacter sp. AR-2-6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.854AlaAla: 5.854 ± 0.095	0.654AlaCys: 0.654 ± 0.025	4.294AlaAsp: 4.294 ± 0.067	4.559AlaGlu: 4.559 ± 0.078	3.491AlaPhe: 3.491 ± 0.058	4.926AlaGly: 4.926 ± 0.075	1.202AlaHis: 1.202 ± 0.035	5.936AlaIle: 5.936 ± 0.08	5.829AlaLys: 5.829 ± 0.08	6.864AlaLeu: 6.864 ± 0.081	1.677AlaMet: 1.677 ± 0.036	4.565AlaAsn: 4.565 ± 0.061	2.125AlaPro: 2.125 ± 0.049	3.034AlaGln: 3.034 ± 0.054	2.089AlaArg: 2.089 ± 0.044	4.17AlaSer: 4.17 ± 0.052	4.23AlaThr: 4.23 ± 0.071	4.641AlaVal: 4.641 ± 0.071	0.709AlaTrp: 0.709 ± 0.026	2.953AlaTyr: 2.953 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.532CysAla: 0.532 ± 0.024	0.124CysCys: 0.124 ± 0.011	0.398CysAsp: 0.398 ± 0.019	0.406CysGlu: 0.406 ± 0.02	0.414CysPhe: 0.414 ± 0.018	0.595CysGly: 0.595 ± 0.025	0.177CysHis: 0.177 ± 0.011	0.603CysIle: 0.603 ± 0.025	0.531CysLys: 0.531 ± 0.023	0.74CysLeu: 0.74 ± 0.027	0.167CysMet: 0.167 ± 0.011	0.396CysAsn: 0.396 ± 0.019	0.281CysPro: 0.281 ± 0.017	0.187CysGln: 0.187 ± 0.013	0.258CysArg: 0.258 ± 0.015	0.497CysSer: 0.497 ± 0.02	0.409CysThr: 0.409 ± 0.019	0.462CysVal: 0.462 ± 0.019	0.067CysTrp: 0.067 ± 0.008	0.291CysTyr: 0.291 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.74AspAla: 3.74 ± 0.054	0.383AspCys: 0.383 ± 0.019	2.623AspAsp: 2.623 ± 0.05	3.826AspGlu: 3.826 ± 0.059	3.355AspPhe: 3.355 ± 0.058	3.732AspGly: 3.732 ± 0.062	0.895AspHis: 0.895 ± 0.028	3.894AspIle: 3.894 ± 0.066	4.085AspLys: 4.085 ± 0.072	5.279AspLeu: 5.279 ± 0.074	1.137AspMet: 1.137 ± 0.033	2.658AspAsn: 2.658 ± 0.047	1.806AspPro: 1.806 ± 0.041	1.84AspGln: 1.84 ± 0.041	1.976AspArg: 1.976 ± 0.04	2.464AspSer: 2.464 ± 0.046	2.441AspThr: 2.441 ± 0.043	3.491AspVal: 3.491 ± 0.059	0.71AspTrp: 0.71 ± 0.027	2.435AspTyr: 2.435 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	4.032GluAla: 4.032 ± 0.076	0.309GluCys: 0.309 ± 0.016	2.889GluAsp: 2.889 ± 0.05	3.958GluGlu: 3.958 ± 0.073	2.736GluPhe: 2.736 ± 0.049	3.235GluGly: 3.235 ± 0.049	1.024GluHis: 1.024 ± 0.027	5.202GluIle: 5.202 ± 0.075	5.301GluLys: 5.301 ± 0.063	5.943GluLeu: 5.943 ± 0.085	1.523GluMet: 1.523 ± 0.038	4.04GluAsn: 4.04 ± 0.062	1.592GluPro: 1.592 ± 0.039	2.55GluGln: 2.55 ± 0.055	2.443GluArg: 2.443 ± 0.051	2.587GluSer: 2.587 ± 0.046	3.217GluThr: 3.217 ± 0.056	3.936GluVal: 3.936 ± 0.057	0.595GluTrp: 0.595 ± 0.023	1.913GluTyr: 1.913 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	3.718PheAla: 3.718 ± 0.058	0.486PheCys: 0.486 ± 0.02	3.055PheAsp: 3.055 ± 0.049	3.02PheGlu: 3.02 ± 0.054	2.569PhePhe: 2.569 ± 0.059	3.61PheGly: 3.61 ± 0.061	0.76PheHis: 0.76 ± 0.026	3.745PheIle: 3.745 ± 0.065	3.814PheLys: 3.814 ± 0.062	4.62PheLeu: 4.62 ± 0.078	1.07PheMet: 1.07 ± 0.029	3.371PheAsn: 3.371 ± 0.059	1.653PhePro: 1.653 ± 0.038	1.308PheGln: 1.308 ± 0.035	1.657PheArg: 1.657 ± 0.04	3.752PheSer: 3.752 ± 0.056	3.208PheThr: 3.208 ± 0.056	3.214PheVal: 3.214 ± 0.058	0.57PheTrp: 0.57 ± 0.022	2.196PheTyr: 2.196 ± 0.052	0.001PheXaa: 0.001 ± 0.001
Gly	4.546GlyAla: 4.546 ± 0.079	0.579GlyCys: 0.579 ± 0.023	3.216GlyAsp: 3.216 ± 0.061	3.446GlyGlu: 3.446 ± 0.058	3.715GlyPhe: 3.715 ± 0.061	4.757GlyGly: 4.757 ± 0.088	1.04GlyHis: 1.04 ± 0.035	5.359GlyIle: 5.359 ± 0.07	5.573GlyLys: 5.573 ± 0.075	6.29GlyLeu: 6.29 ± 0.069	1.58GlyMet: 1.58 ± 0.036	3.759GlyAsn: 3.759 ± 0.062	1.304GlyPro: 1.304 ± 0.035	1.943GlyGln: 1.943 ± 0.043	2.249GlyArg: 2.249 ± 0.047	4.108GlySer: 4.108 ± 0.07	3.927GlyThr: 3.927 ± 0.071	4.35GlyVal: 4.35 ± 0.059	0.814GlyTrp: 0.814 ± 0.027	2.792GlyTyr: 2.792 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	0.975HisAla: 0.975 ± 0.028	0.188HisCys: 0.188 ± 0.013	0.778HisAsp: 0.778 ± 0.026	0.9HisGlu: 0.9 ± 0.028	1.033HisPhe: 1.033 ± 0.031	1.008HisGly: 1.008 ± 0.033	0.475HisHis: 0.475 ± 0.022	1.383HisIle: 1.383 ± 0.034	1.022HisLys: 1.022 ± 0.036	1.775HisLeu: 1.775 ± 0.041	0.283HisMet: 0.283 ± 0.015	0.808HisAsn: 0.808 ± 0.025	0.869HisPro: 0.869 ± 0.029	0.852HisGln: 0.852 ± 0.025	0.636HisArg: 0.636 ± 0.023	0.99HisSer: 0.99 ± 0.029	0.835HisThr: 0.835 ± 0.025	0.844HisVal: 0.844 ± 0.027	0.19HisTrp: 0.19 ± 0.013	0.732HisTyr: 0.732 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.315IleAla: 6.315 ± 0.073	0.697IleCys: 0.697 ± 0.027	4.719IleAsp: 4.719 ± 0.065	4.946IleGlu: 4.946 ± 0.072	3.634IlePhe: 3.634 ± 0.071	5.234IleGly: 5.234 ± 0.07	1.22IleHis: 1.22 ± 0.037	5.908IleIle: 5.908 ± 0.083	6.096IleLys: 6.096 ± 0.076	6.991IleLeu: 6.991 ± 0.081	1.383IleMet: 1.383 ± 0.032	5.077IleAsn: 5.077 ± 0.063	3.015IlePro: 3.015 ± 0.056	2.257IleGln: 2.257 ± 0.048	2.524IleArg: 2.524 ± 0.05	5.333IleSer: 5.333 ± 0.079	4.7IleThr: 4.7 ± 0.067	4.687IleVal: 4.687 ± 0.07	0.751IleTrp: 0.751 ± 0.026	2.825IleTyr: 2.825 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	5.786LysAla: 5.786 ± 0.079	0.352LysCys: 0.352 ± 0.017	4.323LysAsp: 4.323 ± 0.072	5.068LysGlu: 5.068 ± 0.073	3.19LysPhe: 3.19 ± 0.048	4.636LysGly: 4.636 ± 0.057	1.336LysHis: 1.336 ± 0.039	6.175LysIle: 6.175 ± 0.075	6.377LysLys: 6.377 ± 0.081	7.091LysLeu: 7.091 ± 0.081	2.097LysMet: 2.097 ± 0.052	5.326LysAsn: 5.326 ± 0.075	2.906LysPro: 2.906 ± 0.052	3.032LysGln: 3.032 ± 0.052	2.712LysArg: 2.712 ± 0.049	4.525LysSer: 4.525 ± 0.063	4.781LysThr: 4.781 ± 0.056	4.897LysVal: 4.897 ± 0.068	0.847LysTrp: 0.847 ± 0.026	3.067LysTyr: 3.067 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	7.509LeuAla: 7.509 ± 0.087	0.793LeuCys: 0.793 ± 0.024	4.733LeuAsp: 4.733 ± 0.051	4.953LeuGlu: 4.953 ± 0.065	4.805LeuPhe: 4.805 ± 0.084	5.779LeuGly: 5.779 ± 0.078	1.489LeuHis: 1.489 ± 0.041	7.382LeuIle: 7.382 ± 0.097	8.074LeuLys: 8.074 ± 0.088	9.168LeuLeu: 9.168 ± 0.102	2.177LeuMet: 2.177 ± 0.045	6.19LeuAsn: 6.19 ± 0.082	3.912LeuPro: 3.912 ± 0.064	3.45LeuGln: 3.45 ± 0.055	3.285LeuArg: 3.285 ± 0.058	6.654LeuSer: 6.654 ± 0.082	5.696LeuThr: 5.696 ± 0.068	5.492LeuVal: 5.492 ± 0.073	0.887LeuTrp: 0.887 ± 0.03	3.011LeuTyr: 3.011 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	1.727MetAla: 1.727 ± 0.038	0.135MetCys: 0.135 ± 0.011	1.135MetAsp: 1.135 ± 0.03	1.327MetGlu: 1.327 ± 0.03	0.9MetPhe: 0.9 ± 0.029	1.511MetGly: 1.511 ± 0.04	0.379MetHis: 0.379 ± 0.016	1.506MetIle: 1.506 ± 0.037	2.103MetLys: 2.103 ± 0.046	2.112MetLeu: 2.112 ± 0.044	0.67MetMet: 0.67 ± 0.025	1.277MetAsn: 1.277 ± 0.033	1.032MetPro: 1.032 ± 0.029	0.961MetGln: 0.961 ± 0.025	0.865MetArg: 0.865 ± 0.025	1.327MetSer: 1.327 ± 0.034	1.002MetThr: 1.002 ± 0.029	1.489MetVal: 1.489 ± 0.037	0.164MetTrp: 0.164 ± 0.012	0.657MetTyr: 0.657 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	4.514AsnAla: 4.514 ± 0.064	0.44AsnCys: 0.44 ± 0.017	3.077AsnAsp: 3.077 ± 0.048	3.563AsnGlu: 3.563 ± 0.057	3.275AsnPhe: 3.275 ± 0.057	4.306AsnGly: 4.306 ± 0.063	1.031AsnHis: 1.031 ± 0.026	4.767AsnIle: 4.767 ± 0.061	4.355AsnLys: 4.355 ± 0.059	5.866AsnLeu: 5.866 ± 0.071	1.264AsnMet: 1.264 ± 0.033	3.814AsnAsn: 3.814 ± 0.059	2.792AsnPro: 2.792 ± 0.049	2.445AsnGln: 2.445 ± 0.054	2.233AsnArg: 2.233 ± 0.044	3.609AsnSer: 3.609 ± 0.059	3.526AsnThr: 3.526 ± 0.063	3.532AsnVal: 3.532 ± 0.057	0.821AsnTrp: 0.821 ± 0.028	2.864AsnTyr: 2.864 ± 0.056	0.001AsnXaa: 0.001 ± 0.001
Pro	2.62ProAla: 2.62 ± 0.052	0.221ProCys: 0.221 ± 0.013	1.985ProAsp: 1.985 ± 0.045	2.512ProGlu: 2.512 ± 0.043	1.911ProPhe: 1.911 ± 0.039	2.102ProGly: 2.102 ± 0.047	0.571ProHis: 0.571 ± 0.024	2.863ProIle: 2.863 ± 0.059	2.62ProLys: 2.62 ± 0.044	3.099ProLeu: 3.099 ± 0.058	0.785ProMet: 0.785 ± 0.026	2.29ProAsn: 2.29 ± 0.05	0.849ProPro: 0.849 ± 0.032	1.275ProGln: 1.275 ± 0.034	0.935ProArg: 0.935 ± 0.028	2.165ProSer: 2.165 ± 0.046	2.179ProThr: 2.179 ± 0.052	2.484ProVal: 2.484 ± 0.05	0.352ProTrp: 0.352 ± 0.016	1.357ProTyr: 1.357 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	2.41GlnAla: 2.41 ± 0.053	0.163GlnCys: 0.163 ± 0.011	1.616GlnAsp: 1.616 ± 0.04	2.012GlnGlu: 2.012 ± 0.05	1.751GlnPhe: 1.751 ± 0.041	1.857GlnGly: 1.857 ± 0.041	0.652GlnHis: 0.652 ± 0.024	2.987GlnIle: 2.987 ± 0.053	2.981GlnLys: 2.981 ± 0.048	3.838GlnLeu: 3.838 ± 0.06	0.926GlnMet: 0.926 ± 0.03	2.424GlnAsn: 2.424 ± 0.051	1.253GlnPro: 1.253 ± 0.035	1.93GlnGln: 1.93 ± 0.045	1.33GlnArg: 1.33 ± 0.034	2.163GlnSer: 2.163 ± 0.045	2.288GlnThr: 2.288 ± 0.044	2.145GlnVal: 2.145 ± 0.044	0.358GlnTrp: 0.358 ± 0.017	1.375GlnTyr: 1.375 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	2.191ArgAla: 2.191 ± 0.05	0.207ArgCys: 0.207 ± 0.015	1.712ArgAsp: 1.712 ± 0.039	2.039ArgGlu: 2.039 ± 0.042	1.885ArgPhe: 1.885 ± 0.036	2.017ArgGly: 2.017 ± 0.052	0.53ArgHis: 0.53 ± 0.021	2.842ArgIle: 2.842 ± 0.048	2.769ArgLys: 2.769 ± 0.052	3.444ArgLeu: 3.444 ± 0.063	0.903ArgMet: 0.903 ± 0.026	2.221ArgAsn: 2.221 ± 0.044	1.184ArgPro: 1.184 ± 0.029	1.17ArgGln: 1.17 ± 0.035	1.297ArgArg: 1.297 ± 0.036	1.961ArgSer: 1.961 ± 0.046	1.877ArgThr: 1.877 ± 0.036	2.121ArgVal: 2.121 ± 0.041	0.403ArgTrp: 0.403 ± 0.017	1.504ArgTyr: 1.504 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.428SerAla: 4.428 ± 0.07	0.524SerCys: 0.524 ± 0.02	2.863SerAsp: 2.863 ± 0.047	3.11SerGlu: 3.11 ± 0.053	3.698SerPhe: 3.698 ± 0.061	4.421SerGly: 4.421 ± 0.072	0.957SerHis: 0.957 ± 0.027	5.025SerIle: 5.025 ± 0.067	4.367SerLys: 4.367 ± 0.063	6.101SerLeu: 6.101 ± 0.084	1.218SerMet: 1.218 ± 0.033	3.479SerAsn: 3.479 ± 0.053	2.056SerPro: 2.056 ± 0.046	1.912SerGln: 1.912 ± 0.04	2.047SerArg: 2.047 ± 0.043	4.06SerSer: 4.06 ± 0.063	3.532SerThr: 3.532 ± 0.059	3.798SerVal: 3.798 ± 0.064	0.621SerTrp: 0.621 ± 0.023	2.614SerTyr: 2.614 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	4.653ThrAla: 4.653 ± 0.073	0.343ThrCys: 0.343 ± 0.018	3.254ThrAsp: 3.254 ± 0.054	3.264ThrGlu: 3.264 ± 0.046	2.887ThrPhe: 2.887 ± 0.058	4.264ThrGly: 4.264 ± 0.065	0.911ThrHis: 0.911 ± 0.023	4.623ThrIle: 4.623 ± 0.061	3.86ThrLys: 3.86 ± 0.051	5.436ThrLeu: 5.436 ± 0.067	0.958ThrMet: 0.958 ± 0.029	3.285ThrAsn: 3.285 ± 0.06	2.471ThrPro: 2.471 ± 0.048	2.065ThrGln: 2.065 ± 0.045	1.661ThrArg: 1.661 ± 0.043	3.514ThrSer: 3.514 ± 0.06	3.615ThrThr: 3.615 ± 0.082	3.719ThrVal: 3.719 ± 0.06	0.613ThrTrp: 0.613 ± 0.025	2.416ThrTyr: 2.416 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	4.715ValAla: 4.715 ± 0.063	0.506ValCys: 0.506 ± 0.022	3.43ValAsp: 3.43 ± 0.058	3.592ValGlu: 3.592 ± 0.055	3.162ValPhe: 3.162 ± 0.054	3.983ValGly: 3.983 ± 0.067	0.895ValHis: 0.895 ± 0.03	4.752ValIle: 4.752 ± 0.069	5.11ValLys: 5.11 ± 0.068	5.825ValLeu: 5.825 ± 0.089	1.427ValMet: 1.427 ± 0.032	3.945ValAsn: 3.945 ± 0.059	2.226ValPro: 2.226 ± 0.05	1.906ValGln: 1.906 ± 0.038	2.018ValArg: 2.018 ± 0.047	3.979ValSer: 3.979 ± 0.065	3.52ValThr: 3.52 ± 0.062	4.229ValVal: 4.229 ± 0.071	0.669ValTrp: 0.669 ± 0.024	2.306ValTyr: 2.306 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.737TrpAla: 0.737 ± 0.025	0.102TrpCys: 0.102 ± 0.009	0.615TrpAsp: 0.615 ± 0.026	0.553TrpGlu: 0.553 ± 0.022	0.614TrpPhe: 0.614 ± 0.021	0.74TrpGly: 0.74 ± 0.024	0.243TrpHis: 0.243 ± 0.015	0.707TrpIle: 0.707 ± 0.022	0.763TrpLys: 0.763 ± 0.027	1.123TrpLeu: 1.123 ± 0.032	0.317TrpMet: 0.317 ± 0.017	0.65TrpAsn: 0.65 ± 0.026	0.299TrpPro: 0.299 ± 0.016	0.501TrpGln: 0.501 ± 0.023	0.452TrpArg: 0.452 ± 0.023	0.602TrpSer: 0.602 ± 0.024	0.528TrpThr: 0.528 ± 0.026	0.636TrpVal: 0.636 ± 0.025	0.169TrpTrp: 0.169 ± 0.012	0.42TrpTyr: 0.42 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.799TyrAla: 2.799 ± 0.054	0.329TyrCys: 0.329 ± 0.014	2.056TyrAsp: 2.056 ± 0.042	1.972TyrGlu: 1.972 ± 0.04	2.372TyrPhe: 2.372 ± 0.047	2.633TyrGly: 2.633 ± 0.059	0.79TyrHis: 0.79 ± 0.027	2.651TyrIle: 2.651 ± 0.057	2.9TyrLys: 2.9 ± 0.047	3.833TyrLeu: 3.833 ± 0.06	0.714TyrMet: 0.714 ± 0.025	2.52TyrAsn: 2.52 ± 0.046	1.549TyrPro: 1.549 ± 0.035	1.838TyrGln: 1.838 ± 0.043	1.611TyrArg: 1.611 ± 0.043	2.371TyrSer: 2.371 ± 0.051	2.302TyrThr: 2.302 ± 0.053	2.029TyrVal: 2.029 ± 0.042	0.459TyrTrp: 0.459 ± 0.021	1.65TyrTyr: 1.65 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3664 proteins (1228412 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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