Cecembia lonarensis LW9
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4213 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K1L266|K1L266_9BACT Macrolide export ATP-binding/permease protein MacB OS=Cecembia lonarensis LW9 OX=1225176 GN=macB_1 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 10.21 RR3 pKa = 11.84 YY4 pKa = 9.65 IKK6 pKa = 9.25 YY7 pKa = 9.08 TNINLFFIALGLLIIFGNHH26 pKa = 4.11 QKK28 pKa = 10.61 VYY30 pKa = 10.97 AQGFNNNQWIFGYY43 pKa = 10.29 CGSGQEE49 pKa = 3.96 NSYY52 pKa = 11.3 LSFGRR57 pKa = 11.84 GEE59 pKa = 4.27 DD60 pKa = 3.56 PVIRR64 pKa = 11.84 TLPPSILIGDD74 pKa = 3.82 EE75 pKa = 3.95 NGNVDD80 pKa = 3.49 NNAIVIDD87 pKa = 5.1 PITGQILFYY96 pKa = 10.41 TNGVLVYY103 pKa = 10.4 NYY105 pKa = 9.86 DD106 pKa = 3.53 DD107 pKa = 5.0 QIIQGAPNGINGQTDD122 pKa = 3.01 VRR124 pKa = 11.84 QTVAIGTLNYY134 pKa = 9.49 EE135 pKa = 4.28 AEE137 pKa = 4.59 GDD139 pKa = 3.58 RR140 pKa = 11.84 QFYY143 pKa = 10.19 IFHH146 pKa = 6.59 ITPGGQLLYY155 pKa = 10.86 SVVDD159 pKa = 3.71 MNSPGGAQGNAPPLGQVIAFDD180 pKa = 3.68 QLLSNNVSGAISVVKK195 pKa = 9.67 TPQSPSYY202 pKa = 10.34 LISFEE207 pKa = 3.94 GGEE210 pKa = 4.06 LVARR214 pKa = 11.84 RR215 pKa = 11.84 VEE217 pKa = 4.15 ANQGDD222 pKa = 4.25 FTITDD227 pKa = 3.27 NFGFPFTPKK236 pKa = 10.52 AIIFDD241 pKa = 3.85 EE242 pKa = 4.28 SGKK245 pKa = 10.77 LILIPEE251 pKa = 4.52 GDD253 pKa = 3.95 GEE255 pKa = 4.97 DD256 pKa = 3.47 IAVLDD261 pKa = 4.62 FDD263 pKa = 4.39 TSTGSFGNLSFISNSGGNAAIEE285 pKa = 4.29 GAAFSPDD292 pKa = 2.56 GDD294 pKa = 3.9 FIYY297 pKa = 10.27 FSRR300 pKa = 11.84 GDD302 pKa = 3.27 QLFRR306 pKa = 11.84 VPANDD311 pKa = 4.81 LDD313 pKa = 4.1 TDD315 pKa = 4.07 PEE317 pKa = 4.46 VIPLEE322 pKa = 4.12 GDD324 pKa = 2.4 IDD326 pKa = 5.12 AIFDD330 pKa = 3.68 IKK332 pKa = 10.94 VGPDD336 pKa = 2.69 GRR338 pKa = 11.84 LYY340 pKa = 10.73 YY341 pKa = 9.88 IYY343 pKa = 10.13 EE344 pKa = 4.14 EE345 pKa = 4.7 TPGGPQLIGRR355 pKa = 11.84 VNNPDD360 pKa = 3.6 EE361 pKa = 4.59 EE362 pKa = 4.42 DD363 pKa = 3.93 LEE365 pKa = 4.18 EE366 pKa = 6.04 LDD368 pKa = 4.98 IEE370 pKa = 4.19 EE371 pKa = 5.3 DD372 pKa = 3.74 PFNGTDD378 pKa = 3.4 FCGRR382 pKa = 11.84 VFPQFAPNQDD392 pKa = 3.1 INPTVDD398 pKa = 5.18 FDD400 pKa = 3.97 WEE402 pKa = 4.27 PDD404 pKa = 4.16 FPCSNNPVQLTSQITPEE421 pKa = 3.8 NYY423 pKa = 9.55 RR424 pKa = 11.84 PVSFEE429 pKa = 3.71 WTFEE433 pKa = 4.05 PPLTDD438 pKa = 3.35 EE439 pKa = 5.27 DD440 pKa = 4.69 GEE442 pKa = 4.3 PVDD445 pKa = 4.08 IDD447 pKa = 3.81 FNQEE451 pKa = 3.34 HH452 pKa = 6.79 LLIPEE457 pKa = 4.33 EE458 pKa = 4.06 ATANQSIQVTLTVTFADD475 pKa = 4.23 GNVVTVPKK483 pKa = 9.91 TIQLTEE489 pKa = 4.06 NNLEE493 pKa = 4.42 ANFSAQDD500 pKa = 3.42 TTICEE505 pKa = 4.7 GPCVDD510 pKa = 5.04 IGSLLQVQEE519 pKa = 4.41 GGGQGGEE526 pKa = 4.12 NPNVGGPGQGGNYY539 pKa = 9.85 EE540 pKa = 4.36 YY541 pKa = 10.62 FWSNRR546 pKa = 11.84 RR547 pKa = 11.84 DD548 pKa = 3.74 EE549 pKa = 4.42 GWVRR553 pKa = 11.84 DD554 pKa = 3.56 TDD556 pKa = 3.76 NCVDD560 pKa = 3.66 RR561 pKa = 11.84 PGLYY565 pKa = 8.66 WVLVRR570 pKa = 11.84 EE571 pKa = 4.7 PGSEE575 pKa = 3.97 CYY577 pKa = 10.14 AYY579 pKa = 10.63 AEE581 pKa = 4.08 VRR583 pKa = 11.84 VRR585 pKa = 11.84 IWDD588 pKa = 4.75 LPDD591 pKa = 3.0 QSNNVWLFGNGAGLDD606 pKa = 4.04 FNPDD610 pKa = 3.58 PDD612 pKa = 5.11 DD613 pKa = 4.22 EE614 pKa = 4.43 EE615 pKa = 6.48 APLPRR620 pKa = 11.84 PVSHH624 pKa = 6.64 NRR626 pKa = 11.84 DD627 pKa = 3.09 IPAGTTTISDD637 pKa = 3.26 EE638 pKa = 4.24 TGQVLFFTDD647 pKa = 5.18 GEE649 pKa = 4.95 SVWDD653 pKa = 4.04 LNGDD657 pKa = 4.3 LMANGDD663 pKa = 4.1 NIGGNNQASEE673 pKa = 4.39 GVIAVPVPQNQTIFYY688 pKa = 10.36 VFTTQRR694 pKa = 11.84 AADD697 pKa = 3.66 GSNRR701 pKa = 11.84 VSYY704 pKa = 10.91 SVVDD708 pKa = 3.76 IKK710 pKa = 10.38 TDD712 pKa = 3.08 NPTGVGSVVTKK723 pKa = 11.13 NNFLFSPSTEE733 pKa = 3.72 QTAALASGDD742 pKa = 3.95 TTWVLFHH749 pKa = 7.06 EE750 pKa = 5.11 LGNNTFRR757 pKa = 11.84 AYY759 pKa = 9.44 PVSPFGIGSPVFSSVGSNHH778 pKa = 6.05 NFTSGVGSMKK788 pKa = 10.45 FSPDD792 pKa = 3.02 GNQVAVTISDD802 pKa = 4.02 GNCSRR807 pKa = 11.84 LEE809 pKa = 3.56 IFDD812 pKa = 4.87 FNEE815 pKa = 3.57 QTGRR819 pKa = 11.84 LTEE822 pKa = 3.88 YY823 pKa = 11.39 ALIEE827 pKa = 4.34 LGCGAEE833 pKa = 3.96 EE834 pKa = 4.76 VYY836 pKa = 10.94 GLEE839 pKa = 4.09 FSPDD843 pKa = 2.86 GSRR846 pKa = 11.84 IFVSYY851 pKa = 9.58 TGSPGKK857 pKa = 9.98 IEE859 pKa = 4.27 EE860 pKa = 4.39 YY861 pKa = 9.99 LVKK864 pKa = 10.48 RR865 pKa = 11.84 PEE867 pKa = 4.1 TAVEE871 pKa = 4.32 GEE873 pKa = 5.19 DD874 pKa = 3.28 IDD876 pKa = 5.83 SDD878 pKa = 4.48 LSCFDD883 pKa = 3.85 CFEE886 pKa = 4.34 NASNRR891 pKa = 11.84 SQRR894 pKa = 11.84 EE895 pKa = 3.38 NCIIDD900 pKa = 3.58 SRR902 pKa = 11.84 NVLSTAGPFGAIQIGPDD919 pKa = 3.28 GQIYY923 pKa = 8.61 VARR926 pKa = 11.84 PDD928 pKa = 3.62 RR929 pKa = 11.84 NEE931 pKa = 3.36 ITAILPGFNCLDD943 pKa = 3.25 SFYY946 pKa = 10.96 NEE948 pKa = 4.08 QGVSLLPGTSMNLGLPAFVQQSGSSIPEE976 pKa = 3.67 PALAGIEE983 pKa = 4.07 RR984 pKa = 11.84 LCFDD988 pKa = 4.43 PDD990 pKa = 3.27 GTIGLFEE997 pKa = 5.74 AGGEE1001 pKa = 4.0 PDD1003 pKa = 2.99 IDD1005 pKa = 4.01 SYY1007 pKa = 11.62 FWTITHH1013 pKa = 6.97 EE1014 pKa = 4.53 DD1015 pKa = 3.91 GEE1017 pKa = 5.16 VVFEE1021 pKa = 4.24 EE1022 pKa = 5.04 GGPGEE1027 pKa = 4.34 TFQEE1031 pKa = 4.24 IEE1033 pKa = 4.02 PLFPRR1038 pKa = 11.84 VGIYY1042 pKa = 8.97 TVALRR1047 pKa = 11.84 VDD1049 pKa = 3.21 RR1050 pKa = 11.84 CGNPEE1055 pKa = 3.89 YY1056 pKa = 10.37 YY1057 pKa = 9.6 RR1058 pKa = 11.84 EE1059 pKa = 4.11 SMEE1062 pKa = 4.19 VEE1064 pKa = 4.64 VIASPEE1070 pKa = 3.82 LTLPEE1075 pKa = 4.35 EE1076 pKa = 4.26 ATLCVGTPVPLTAIDD1091 pKa = 4.93 GYY1093 pKa = 11.26 DD1094 pKa = 3.69 PSEE1097 pKa = 3.87 GLYY1100 pKa = 10.91 DD1101 pKa = 3.92 FEE1103 pKa = 4.48 WRR1105 pKa = 11.84 NAAGQLIGDD1114 pKa = 4.19 EE1115 pKa = 4.15 NSNTIEE1121 pKa = 4.03 VVEE1124 pKa = 4.17 EE1125 pKa = 3.83 SVYY1128 pKa = 10.17 TVTVRR1133 pKa = 11.84 FRR1135 pKa = 11.84 VPEE1138 pKa = 4.19 GVDD1141 pKa = 3.2 PEE1143 pKa = 4.78 EE1144 pKa = 5.01 FDD1146 pKa = 3.52 ACPVSRR1152 pKa = 11.84 SIFVGPAFDD1161 pKa = 4.66 FDD1163 pKa = 5.56 LIQSAEE1169 pKa = 4.3 EE1170 pKa = 4.12 VCLDD1174 pKa = 3.63 EE1175 pKa = 4.6 PVVIFSPNTPITGEE1189 pKa = 3.9 WFFRR1193 pKa = 11.84 PAGSPDD1199 pKa = 3.32 RR1200 pKa = 11.84 VSLGEE1205 pKa = 3.94 FFEE1208 pKa = 5.64 LEE1210 pKa = 4.9 LIPASLPSPGDD1221 pKa = 3.3 YY1222 pKa = 10.73 EE1223 pKa = 4.79 IIFFAEE1229 pKa = 4.27 DD1230 pKa = 3.9 PLVEE1234 pKa = 4.22 GCFVEE1239 pKa = 5.65 KK1240 pKa = 10.28 IVPLTIFPLPAVDD1253 pKa = 5.73 AVILSDD1259 pKa = 5.71 AEE1261 pKa = 4.11 DD1262 pKa = 4.29 CEE1264 pKa = 4.73 IPDD1267 pKa = 3.61 GSFEE1271 pKa = 3.98 ITALSDD1277 pKa = 3.45 IEE1279 pKa = 4.19 SLEE1282 pKa = 3.88 VLEE1285 pKa = 5.39 LGLLFGPIAAGEE1297 pKa = 4.07 ALPPFTDD1304 pKa = 4.8 LEE1306 pKa = 4.77 PGLYY1310 pKa = 8.48 TLQMLNGFGCEE1321 pKa = 4.77 FIQSVTIRR1329 pKa = 11.84 NLNPPRR1335 pKa = 11.84 GIEE1338 pKa = 4.26 GYY1340 pKa = 8.36 EE1341 pKa = 3.87 VEE1343 pKa = 4.92 VSPEE1347 pKa = 3.63 ICDD1350 pKa = 3.49 VNGVLDD1356 pKa = 4.07 GQLTIRR1362 pKa = 11.84 FISGGASGTYY1372 pKa = 9.93 QITRR1376 pKa = 11.84 QGDD1379 pKa = 3.61 GAFVSDD1385 pKa = 3.77 NFNDD1389 pKa = 3.59 VEE1391 pKa = 4.63 EE1392 pKa = 4.34 ITVPVPAGIYY1402 pKa = 9.39 SVVVEE1407 pKa = 4.96 DD1408 pKa = 4.16 VDD1410 pKa = 4.07 GCAVPFEE1417 pKa = 4.71 DD1418 pKa = 3.78 LVEE1421 pKa = 4.15 VEE1423 pKa = 4.33 EE1424 pKa = 4.35 VEE1426 pKa = 4.37 IVAFSAPGNVEE1437 pKa = 3.61 ACEE1440 pKa = 4.29 SFTFSPQSEE1449 pKa = 4.55 DD1450 pKa = 3.25 EE1451 pKa = 4.21 LVYY1454 pKa = 9.84 TLRR1457 pKa = 11.84 DD1458 pKa = 3.42 AQGNQIQATPEE1469 pKa = 3.89 GSFTITEE1476 pKa = 4.04 AGLYY1480 pKa = 9.91 VLTGEE1485 pKa = 4.9 DD1486 pKa = 3.8 PDD1488 pKa = 4.8 GIKK1491 pKa = 10.34 CARR1494 pKa = 11.84 EE1495 pKa = 3.54 RR1496 pKa = 11.84 EE1497 pKa = 4.35 INVTLTTAPVFSLAGPRR1514 pKa = 11.84 VDD1516 pKa = 4.57 CDD1518 pKa = 3.3 LGLFYY1523 pKa = 10.72 EE1524 pKa = 4.97 AVLGPNEE1531 pKa = 4.94 DD1532 pKa = 3.92 PNQVFIFWLDD1542 pKa = 3.45 SNGEE1546 pKa = 4.33 VVSRR1550 pKa = 11.84 NPLFFPRR1557 pKa = 11.84 VPGTYY1562 pKa = 9.67 FLEE1565 pKa = 4.34 VQPRR1569 pKa = 11.84 TGSLCEE1575 pKa = 3.84 IDD1577 pKa = 4.85 PISFTVEE1584 pKa = 3.86 DD1585 pKa = 4.62 TDD1587 pKa = 3.8 PQIDD1591 pKa = 3.97 VEE1593 pKa = 4.88 LEE1595 pKa = 3.81 ALPFCAEE1602 pKa = 4.54 DD1603 pKa = 3.94 PFTLISVIADD1613 pKa = 3.84 LSDD1616 pKa = 3.15 VALIQWFEE1624 pKa = 4.17 IINGEE1629 pKa = 4.29 RR1630 pKa = 11.84 EE1631 pKa = 4.64 LIEE1634 pKa = 4.52 DD1635 pKa = 4.85 WIGLDD1640 pKa = 3.16 QVVALFEE1647 pKa = 4.54 GVFEE1651 pKa = 4.53 IVLINNDD1658 pKa = 2.85 GCEE1661 pKa = 3.76 IGTAIIEE1668 pKa = 4.22 IRR1670 pKa = 11.84 QSTIGPPDD1678 pKa = 3.98 LDD1680 pKa = 3.75 EE1681 pKa = 6.07 LYY1683 pKa = 10.79 VICAPEE1689 pKa = 4.99 GILVEE1694 pKa = 4.56 IGPGEE1699 pKa = 3.95 YY1700 pKa = 10.42 DD1701 pKa = 3.11 NYY1703 pKa = 10.86 QWILDD1708 pKa = 4.02 EE1709 pKa = 4.33 EE1710 pKa = 4.9 VVAVSPTFSPTIPGDD1725 pKa = 3.5 YY1726 pKa = 10.48 EE1727 pKa = 5.84 LIVSDD1732 pKa = 5.62 DD1733 pKa = 3.94 LGCEE1737 pKa = 3.7 FRR1739 pKa = 11.84 ARR1741 pKa = 11.84 FVVEE1745 pKa = 4.29 EE1746 pKa = 4.09 DD1747 pKa = 4.04 CEE1749 pKa = 4.44 LKK1751 pKa = 10.79 INFPNAIIPNDD1762 pKa = 3.49 SEE1764 pKa = 5.02 RR1765 pKa = 11.84 NFVVYY1770 pKa = 10.49 TNDD1773 pKa = 3.31 FVDD1776 pKa = 3.52 EE1777 pKa = 4.74 LEE1779 pKa = 4.17 VFIYY1783 pKa = 10.45 NRR1785 pKa = 11.84 WGEE1788 pKa = 4.22 LIFYY1792 pKa = 10.26 CEE1794 pKa = 4.29 SNGVNGEE1801 pKa = 4.05 IPRR1804 pKa = 11.84 CAWDD1808 pKa = 4.37 GIVNGRR1814 pKa = 11.84 TVPIGTYY1821 pKa = 9.46 PVVVNYY1827 pKa = 10.51 GSTSQNVRR1835 pKa = 11.84 KK1836 pKa = 7.68 TLKK1839 pKa = 9.52 RR1840 pKa = 11.84 TILVIEE1846 pKa = 4.2
Molecular weight: 201.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.554
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.63
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.834
DTASelect 4.037
Thurlkill 3.579
EMBOSS 3.643
Sillero 3.884
Patrickios 1.24
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|K1LBU4|K1LBU4_9BACT Histidine protein kinase divJ OS=Cecembia lonarensis LW9 OX=1225176 GN=divJ_1 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.78 HH16 pKa = 3.85 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 RR29 pKa = 11.84 VIKK32 pKa = 10.16 SRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 HH40 pKa = 5.41 KK41 pKa = 10.43 LTVSSEE47 pKa = 4.01 KK48 pKa = 9.88 TLKK51 pKa = 10.51 KK52 pKa = 10.72
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.471
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.193
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.07
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4213
0
4213
1388209
29
2681
329.5
37.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.745 ± 0.035
0.688 ± 0.011
5.37 ± 0.029
7.001 ± 0.036
5.516 ± 0.034
7.07 ± 0.036
1.939 ± 0.018
7.283 ± 0.032
6.686 ± 0.043
9.903 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.53 ± 0.019
5.065 ± 0.033
3.99 ± 0.025
3.753 ± 0.021
4.293 ± 0.026
6.131 ± 0.025
4.798 ± 0.024
6.228 ± 0.03
1.289 ± 0.016
3.723 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here