Cecembia lonarensis LW9

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Cecembia; Cecembia lonarensis

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|K1L266|K1L266_9BACT Macrolide export ATP-binding/permease protein MacB OS=Cecembia lonarensis LW9 OX=1225176 GN=macB_1 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.21RR3 pKa = 11.84YY4 pKa = 9.65IKK6 pKa = 9.25YY7 pKa = 9.08TNINLFFIALGLLIIFGNHH26 pKa = 4.11QKK28 pKa = 10.61VYY30 pKa = 10.97AQGFNNNQWIFGYY43 pKa = 10.29CGSGQEE49 pKa = 3.96NSYY52 pKa = 11.3LSFGRR57 pKa = 11.84GEE59 pKa = 4.27DD60 pKa = 3.56PVIRR64 pKa = 11.84TLPPSILIGDD74 pKa = 3.82EE75 pKa = 3.95NGNVDD80 pKa = 3.49NNAIVIDD87 pKa = 5.1PITGQILFYY96 pKa = 10.41TNGVLVYY103 pKa = 10.4NYY105 pKa = 9.86DD106 pKa = 3.53DD107 pKa = 5.0QIIQGAPNGINGQTDD122 pKa = 3.01VRR124 pKa = 11.84QTVAIGTLNYY134 pKa = 9.49EE135 pKa = 4.28AEE137 pKa = 4.59GDD139 pKa = 3.58RR140 pKa = 11.84QFYY143 pKa = 10.19IFHH146 pKa = 6.59ITPGGQLLYY155 pKa = 10.86SVVDD159 pKa = 3.71MNSPGGAQGNAPPLGQVIAFDD180 pKa = 3.68QLLSNNVSGAISVVKK195 pKa = 9.67TPQSPSYY202 pKa = 10.34LISFEE207 pKa = 3.94GGEE210 pKa = 4.06LVARR214 pKa = 11.84RR215 pKa = 11.84VEE217 pKa = 4.15ANQGDD222 pKa = 4.25FTITDD227 pKa = 3.27NFGFPFTPKK236 pKa = 10.52AIIFDD241 pKa = 3.85EE242 pKa = 4.28SGKK245 pKa = 10.77LILIPEE251 pKa = 4.52GDD253 pKa = 3.95GEE255 pKa = 4.97DD256 pKa = 3.47IAVLDD261 pKa = 4.62FDD263 pKa = 4.39TSTGSFGNLSFISNSGGNAAIEE285 pKa = 4.29GAAFSPDD292 pKa = 2.56GDD294 pKa = 3.9FIYY297 pKa = 10.27FSRR300 pKa = 11.84GDD302 pKa = 3.27QLFRR306 pKa = 11.84VPANDD311 pKa = 4.81LDD313 pKa = 4.1TDD315 pKa = 4.07PEE317 pKa = 4.46VIPLEE322 pKa = 4.12GDD324 pKa = 2.4IDD326 pKa = 5.12AIFDD330 pKa = 3.68IKK332 pKa = 10.94VGPDD336 pKa = 2.69GRR338 pKa = 11.84LYY340 pKa = 10.73YY341 pKa = 9.88IYY343 pKa = 10.13EE344 pKa = 4.14EE345 pKa = 4.7TPGGPQLIGRR355 pKa = 11.84VNNPDD360 pKa = 3.6EE361 pKa = 4.59EE362 pKa = 4.42DD363 pKa = 3.93LEE365 pKa = 4.18EE366 pKa = 6.04LDD368 pKa = 4.98IEE370 pKa = 4.19EE371 pKa = 5.3DD372 pKa = 3.74PFNGTDD378 pKa = 3.4FCGRR382 pKa = 11.84VFPQFAPNQDD392 pKa = 3.1INPTVDD398 pKa = 5.18FDD400 pKa = 3.97WEE402 pKa = 4.27PDD404 pKa = 4.16FPCSNNPVQLTSQITPEE421 pKa = 3.8NYY423 pKa = 9.55RR424 pKa = 11.84PVSFEE429 pKa = 3.71WTFEE433 pKa = 4.05PPLTDD438 pKa = 3.35EE439 pKa = 5.27DD440 pKa = 4.69GEE442 pKa = 4.3PVDD445 pKa = 4.08IDD447 pKa = 3.81FNQEE451 pKa = 3.34HH452 pKa = 6.79LLIPEE457 pKa = 4.33EE458 pKa = 4.06ATANQSIQVTLTVTFADD475 pKa = 4.23GNVVTVPKK483 pKa = 9.91TIQLTEE489 pKa = 4.06NNLEE493 pKa = 4.42ANFSAQDD500 pKa = 3.42TTICEE505 pKa = 4.7GPCVDD510 pKa = 5.04IGSLLQVQEE519 pKa = 4.41GGGQGGEE526 pKa = 4.12NPNVGGPGQGGNYY539 pKa = 9.85EE540 pKa = 4.36YY541 pKa = 10.62FWSNRR546 pKa = 11.84RR547 pKa = 11.84DD548 pKa = 3.74EE549 pKa = 4.42GWVRR553 pKa = 11.84DD554 pKa = 3.56TDD556 pKa = 3.76NCVDD560 pKa = 3.66RR561 pKa = 11.84PGLYY565 pKa = 8.66WVLVRR570 pKa = 11.84EE571 pKa = 4.7PGSEE575 pKa = 3.97CYY577 pKa = 10.14AYY579 pKa = 10.63AEE581 pKa = 4.08VRR583 pKa = 11.84VRR585 pKa = 11.84IWDD588 pKa = 4.75LPDD591 pKa = 3.0QSNNVWLFGNGAGLDD606 pKa = 4.04FNPDD610 pKa = 3.58PDD612 pKa = 5.11DD613 pKa = 4.22EE614 pKa = 4.43EE615 pKa = 6.48APLPRR620 pKa = 11.84PVSHH624 pKa = 6.64NRR626 pKa = 11.84DD627 pKa = 3.09IPAGTTTISDD637 pKa = 3.26EE638 pKa = 4.24TGQVLFFTDD647 pKa = 5.18GEE649 pKa = 4.95SVWDD653 pKa = 4.04LNGDD657 pKa = 4.3LMANGDD663 pKa = 4.1NIGGNNQASEE673 pKa = 4.39GVIAVPVPQNQTIFYY688 pKa = 10.36VFTTQRR694 pKa = 11.84AADD697 pKa = 3.66GSNRR701 pKa = 11.84VSYY704 pKa = 10.91SVVDD708 pKa = 3.76IKK710 pKa = 10.38TDD712 pKa = 3.08NPTGVGSVVTKK723 pKa = 11.13NNFLFSPSTEE733 pKa = 3.72QTAALASGDD742 pKa = 3.95TTWVLFHH749 pKa = 7.06EE750 pKa = 5.11LGNNTFRR757 pKa = 11.84AYY759 pKa = 9.44PVSPFGIGSPVFSSVGSNHH778 pKa = 6.05NFTSGVGSMKK788 pKa = 10.45FSPDD792 pKa = 3.02GNQVAVTISDD802 pKa = 4.02GNCSRR807 pKa = 11.84LEE809 pKa = 3.56IFDD812 pKa = 4.87FNEE815 pKa = 3.57QTGRR819 pKa = 11.84LTEE822 pKa = 3.88YY823 pKa = 11.39ALIEE827 pKa = 4.34LGCGAEE833 pKa = 3.96EE834 pKa = 4.76VYY836 pKa = 10.94GLEE839 pKa = 4.09FSPDD843 pKa = 2.86GSRR846 pKa = 11.84IFVSYY851 pKa = 9.58TGSPGKK857 pKa = 9.98IEE859 pKa = 4.27EE860 pKa = 4.39YY861 pKa = 9.99LVKK864 pKa = 10.48RR865 pKa = 11.84PEE867 pKa = 4.1TAVEE871 pKa = 4.32GEE873 pKa = 5.19DD874 pKa = 3.28IDD876 pKa = 5.83SDD878 pKa = 4.48LSCFDD883 pKa = 3.85CFEE886 pKa = 4.34NASNRR891 pKa = 11.84SQRR894 pKa = 11.84EE895 pKa = 3.38NCIIDD900 pKa = 3.58SRR902 pKa = 11.84NVLSTAGPFGAIQIGPDD919 pKa = 3.28GQIYY923 pKa = 8.61VARR926 pKa = 11.84PDD928 pKa = 3.62RR929 pKa = 11.84NEE931 pKa = 3.36ITAILPGFNCLDD943 pKa = 3.25SFYY946 pKa = 10.96NEE948 pKa = 4.08QGVSLLPGTSMNLGLPAFVQQSGSSIPEE976 pKa = 3.67PALAGIEE983 pKa = 4.07RR984 pKa = 11.84LCFDD988 pKa = 4.43PDD990 pKa = 3.27GTIGLFEE997 pKa = 5.74AGGEE1001 pKa = 4.0PDD1003 pKa = 2.99IDD1005 pKa = 4.01SYY1007 pKa = 11.62FWTITHH1013 pKa = 6.97EE1014 pKa = 4.53DD1015 pKa = 3.91GEE1017 pKa = 5.16VVFEE1021 pKa = 4.24EE1022 pKa = 5.04GGPGEE1027 pKa = 4.34TFQEE1031 pKa = 4.24IEE1033 pKa = 4.02PLFPRR1038 pKa = 11.84VGIYY1042 pKa = 8.97TVALRR1047 pKa = 11.84VDD1049 pKa = 3.21RR1050 pKa = 11.84CGNPEE1055 pKa = 3.89YY1056 pKa = 10.37YY1057 pKa = 9.6RR1058 pKa = 11.84EE1059 pKa = 4.11SMEE1062 pKa = 4.19VEE1064 pKa = 4.64VIASPEE1070 pKa = 3.82LTLPEE1075 pKa = 4.35EE1076 pKa = 4.26ATLCVGTPVPLTAIDD1091 pKa = 4.93GYY1093 pKa = 11.26DD1094 pKa = 3.69PSEE1097 pKa = 3.87GLYY1100 pKa = 10.91DD1101 pKa = 3.92FEE1103 pKa = 4.48WRR1105 pKa = 11.84NAAGQLIGDD1114 pKa = 4.19EE1115 pKa = 4.15NSNTIEE1121 pKa = 4.03VVEE1124 pKa = 4.17EE1125 pKa = 3.83SVYY1128 pKa = 10.17TVTVRR1133 pKa = 11.84FRR1135 pKa = 11.84VPEE1138 pKa = 4.19GVDD1141 pKa = 3.2PEE1143 pKa = 4.78EE1144 pKa = 5.01FDD1146 pKa = 3.52ACPVSRR1152 pKa = 11.84SIFVGPAFDD1161 pKa = 4.66FDD1163 pKa = 5.56LIQSAEE1169 pKa = 4.3EE1170 pKa = 4.12VCLDD1174 pKa = 3.63EE1175 pKa = 4.6PVVIFSPNTPITGEE1189 pKa = 3.9WFFRR1193 pKa = 11.84PAGSPDD1199 pKa = 3.32RR1200 pKa = 11.84VSLGEE1205 pKa = 3.94FFEE1208 pKa = 5.64LEE1210 pKa = 4.9LIPASLPSPGDD1221 pKa = 3.3YY1222 pKa = 10.73EE1223 pKa = 4.79IIFFAEE1229 pKa = 4.27DD1230 pKa = 3.9PLVEE1234 pKa = 4.22GCFVEE1239 pKa = 5.65KK1240 pKa = 10.28IVPLTIFPLPAVDD1253 pKa = 5.73AVILSDD1259 pKa = 5.71AEE1261 pKa = 4.11DD1262 pKa = 4.29CEE1264 pKa = 4.73IPDD1267 pKa = 3.61GSFEE1271 pKa = 3.98ITALSDD1277 pKa = 3.45IEE1279 pKa = 4.19SLEE1282 pKa = 3.88VLEE1285 pKa = 5.39LGLLFGPIAAGEE1297 pKa = 4.07ALPPFTDD1304 pKa = 4.8LEE1306 pKa = 4.77PGLYY1310 pKa = 8.48TLQMLNGFGCEE1321 pKa = 4.77FIQSVTIRR1329 pKa = 11.84NLNPPRR1335 pKa = 11.84GIEE1338 pKa = 4.26GYY1340 pKa = 8.36EE1341 pKa = 3.87VEE1343 pKa = 4.92VSPEE1347 pKa = 3.63ICDD1350 pKa = 3.49VNGVLDD1356 pKa = 4.07GQLTIRR1362 pKa = 11.84FISGGASGTYY1372 pKa = 9.93QITRR1376 pKa = 11.84QGDD1379 pKa = 3.61GAFVSDD1385 pKa = 3.77NFNDD1389 pKa = 3.59VEE1391 pKa = 4.63EE1392 pKa = 4.34ITVPVPAGIYY1402 pKa = 9.39SVVVEE1407 pKa = 4.96DD1408 pKa = 4.16VDD1410 pKa = 4.07GCAVPFEE1417 pKa = 4.71DD1418 pKa = 3.78LVEE1421 pKa = 4.15VEE1423 pKa = 4.33EE1424 pKa = 4.35VEE1426 pKa = 4.37IVAFSAPGNVEE1437 pKa = 3.61ACEE1440 pKa = 4.29SFTFSPQSEE1449 pKa = 4.55DD1450 pKa = 3.25EE1451 pKa = 4.21LVYY1454 pKa = 9.84TLRR1457 pKa = 11.84DD1458 pKa = 3.42AQGNQIQATPEE1469 pKa = 3.89GSFTITEE1476 pKa = 4.04AGLYY1480 pKa = 9.91VLTGEE1485 pKa = 4.9DD1486 pKa = 3.8PDD1488 pKa = 4.8GIKK1491 pKa = 10.34CARR1494 pKa = 11.84EE1495 pKa = 3.54RR1496 pKa = 11.84EE1497 pKa = 4.35INVTLTTAPVFSLAGPRR1514 pKa = 11.84VDD1516 pKa = 4.57CDD1518 pKa = 3.3LGLFYY1523 pKa = 10.72EE1524 pKa = 4.97AVLGPNEE1531 pKa = 4.94DD1532 pKa = 3.92PNQVFIFWLDD1542 pKa = 3.45SNGEE1546 pKa = 4.33VVSRR1550 pKa = 11.84NPLFFPRR1557 pKa = 11.84VPGTYY1562 pKa = 9.67FLEE1565 pKa = 4.34VQPRR1569 pKa = 11.84TGSLCEE1575 pKa = 3.84IDD1577 pKa = 4.85PISFTVEE1584 pKa = 3.86DD1585 pKa = 4.62TDD1587 pKa = 3.8PQIDD1591 pKa = 3.97VEE1593 pKa = 4.88LEE1595 pKa = 3.81ALPFCAEE1602 pKa = 4.54DD1603 pKa = 3.94PFTLISVIADD1613 pKa = 3.84LSDD1616 pKa = 3.15VALIQWFEE1624 pKa = 4.17IINGEE1629 pKa = 4.29RR1630 pKa = 11.84EE1631 pKa = 4.64LIEE1634 pKa = 4.52DD1635 pKa = 4.85WIGLDD1640 pKa = 3.16QVVALFEE1647 pKa = 4.54GVFEE1651 pKa = 4.53IVLINNDD1658 pKa = 2.85GCEE1661 pKa = 3.76IGTAIIEE1668 pKa = 4.22IRR1670 pKa = 11.84QSTIGPPDD1678 pKa = 3.98LDD1680 pKa = 3.75EE1681 pKa = 6.07LYY1683 pKa = 10.79VICAPEE1689 pKa = 4.99GILVEE1694 pKa = 4.56IGPGEE1699 pKa = 3.95YY1700 pKa = 10.42DD1701 pKa = 3.11NYY1703 pKa = 10.86QWILDD1708 pKa = 4.02EE1709 pKa = 4.33EE1710 pKa = 4.9VVAVSPTFSPTIPGDD1725 pKa = 3.5YY1726 pKa = 10.48EE1727 pKa = 5.84LIVSDD1732 pKa = 5.62DD1733 pKa = 3.94LGCEE1737 pKa = 3.7FRR1739 pKa = 11.84ARR1741 pKa = 11.84FVVEE1745 pKa = 4.29EE1746 pKa = 4.09DD1747 pKa = 4.04CEE1749 pKa = 4.44LKK1751 pKa = 10.79INFPNAIIPNDD1762 pKa = 3.49SEE1764 pKa = 5.02RR1765 pKa = 11.84NFVVYY1770 pKa = 10.49TNDD1773 pKa = 3.31FVDD1776 pKa = 3.52EE1777 pKa = 4.74LEE1779 pKa = 4.17VFIYY1783 pKa = 10.45NRR1785 pKa = 11.84WGEE1788 pKa = 4.22LIFYY1792 pKa = 10.26CEE1794 pKa = 4.29SNGVNGEE1801 pKa = 4.05IPRR1804 pKa = 11.84CAWDD1808 pKa = 4.37GIVNGRR1814 pKa = 11.84TVPIGTYY1821 pKa = 9.46PVVVNYY1827 pKa = 10.51GSTSQNVRR1835 pKa = 11.84KK1836 pKa = 7.68TLKK1839 pKa = 9.52RR1840 pKa = 11.84TILVIEE1846 pKa = 4.2

Molecular weight:
201.82 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|K1LBU4|K1LBU4_9BACT Histidine protein kinase divJ OS=Cecembia lonarensis LW9 OX=1225176 GN=divJ_1 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.78HH16 pKa = 3.85GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTANGRR28 pKa = 11.84RR29 pKa = 11.84VIKK32 pKa = 10.16SRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84HH40 pKa = 5.41KK41 pKa = 10.43LTVSSEE47 pKa = 4.01KK48 pKa = 9.88TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight:
6.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4213

0

4213

1388209

29

2681

329.5

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.745 ± 0.035

0.688 ± 0.011

5.37 ± 0.029

7.001 ± 0.036

5.516 ± 0.034

7.07 ± 0.036

1.939 ± 0.018

7.283 ± 0.032

6.686 ± 0.043

9.903 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.019

5.065 ± 0.033

3.99 ± 0.025

3.753 ± 0.021

4.293 ± 0.026

6.131 ± 0.025

4.798 ± 0.024

6.228 ± 0.03

1.289 ± 0.016

3.723 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski