Capronia epimyces CBS 606.96
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10469 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W9YJX4|W9YJX4_9EURO Translation initiation factor 2 subunit 1 OS=Capronia epimyces CBS 606.96 OX=1182542 GN=A1O3_06382 PE=3 SV=1
MM1 pKa = 7.62 LQRR4 pKa = 11.84 QKK6 pKa = 11.55 DD7 pKa = 3.76 GVLTLKK13 pKa = 10.92 LSGSVLTDD21 pKa = 3.09 QADD24 pKa = 3.83 RR25 pKa = 11.84 TGYY28 pKa = 9.72 VAANYY33 pKa = 8.86 QFQFDD38 pKa = 4.53 APPQAGAIYY47 pKa = 9.51 TSGFSLCSNNSLALGGSAIWYY68 pKa = 6.87 EE69 pKa = 4.22 CLSGSFYY76 pKa = 11.37 NLYY79 pKa = 9.73 DD80 pKa = 4.0 RR81 pKa = 11.84 KK82 pKa = 9.8 WADD85 pKa = 3.1 HH86 pKa = 6.64 CFPIYY91 pKa = 10.37 LVALIGSSAPPATQASDD108 pKa = 3.66 GQPAATTVVPSITQISDD125 pKa = 3.15 GQPQVTSAVVTQISDD140 pKa = 3.6 GQPQGNTAVVTQISDD155 pKa = 3.85 GQPQAATATGAPVSQISDD173 pKa = 3.86 GQPQAPTGSPVSQISDD189 pKa = 3.82 GQPQAPTGSPVSQISDD205 pKa = 3.82 GQPQAPTGAPVSQISDD221 pKa = 3.86 GQPQAPTATATPVSQISDD239 pKa = 3.92 GQPQAPKK246 pKa = 8.88 GTPVSQISDD255 pKa = 3.98 GQPQAPTGAPVSQISDD271 pKa = 3.86 GQPQAPTAIPVSQISDD287 pKa = 3.85 GQPQAPTGAPVSQISDD303 pKa = 3.86 GQPQAPTATPVSQISDD319 pKa = 3.98 GQPQAPTATVEE330 pKa = 4.08 AASATGTSSAAPATYY345 pKa = 9.62 TGAAPRR351 pKa = 11.84 DD352 pKa = 3.92 TLSGSLAVAAGIVGAIALLL371 pKa = 3.88
Molecular weight: 36.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.478
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.757
Rodwell 3.554
Grimsley 3.389
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.215
Thurlkill 3.567
EMBOSS 3.757
Sillero 3.859
Patrickios 0.693
IPC_peptide 3.732
IPC2_peptide 3.821
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|W9XQD0|W9XQD0_9EURO HpcH_HpaI domain-containing protein OS=Capronia epimyces CBS 606.96 OX=1182542 GN=A1O3_07809 PE=4 SV=1
MM1 pKa = 7.31 TRR3 pKa = 11.84 LKK5 pKa = 10.99 NLLPLRR11 pKa = 11.84 NQLPPLILLLKK22 pKa = 10.02 NAPLLRR28 pKa = 11.84 NQIPLKK34 pKa = 10.84 NLLQPRR40 pKa = 11.84 KK41 pKa = 9.46 RR42 pKa = 11.84 PRR44 pKa = 11.84 LKK46 pKa = 10.84 SPLLLKK52 pKa = 10.49 RR53 pKa = 11.84 PPPLMLLKK61 pKa = 10.46 NRR63 pKa = 11.84 PRR65 pKa = 11.84 LKK67 pKa = 10.48 SLPPLMLLPKK77 pKa = 10.08 NRR79 pKa = 11.84 PRR81 pKa = 11.84 LKK83 pKa = 10.69 SLLPLMLLPKK93 pKa = 10.24 NRR95 pKa = 11.84 PLLKK99 pKa = 10.44 NQLLLRR105 pKa = 11.84 IRR107 pKa = 11.84 PRR109 pKa = 11.84 LKK111 pKa = 10.71 NLLPLKK117 pKa = 10.63 NLLPPLKK124 pKa = 10.35 NRR126 pKa = 11.84 PRR128 pKa = 11.84 QKK130 pKa = 10.8 NLLRR134 pKa = 11.84 RR135 pKa = 11.84 KK136 pKa = 9.34 NN137 pKa = 3.39
Molecular weight: 16.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.484
IPC2_protein 11.52
IPC_protein 13.1
Toseland 13.261
ProMoST 13.759
Dawson 13.276
Bjellqvist 13.261
Wikipedia 13.744
Rodwell 13.042
Grimsley 13.305
Solomon 13.759
Lehninger 13.656
Nozaki 13.261
DTASelect 13.261
Thurlkill 13.261
EMBOSS 13.759
Sillero 13.261
Patrickios 12.749
IPC_peptide 13.759
IPC2_peptide 12.749
IPC2.peptide.svr19 9.362
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10469
0
10469
5150417
50
5422
492.0
54.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.864 ± 0.02
1.16 ± 0.009
5.789 ± 0.015
6.04 ± 0.021
3.634 ± 0.016
6.917 ± 0.02
2.509 ± 0.01
4.721 ± 0.016
4.62 ± 0.022
9.078 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.036 ± 0.008
3.445 ± 0.012
6.117 ± 0.023
4.182 ± 0.017
6.227 ± 0.02
8.151 ± 0.025
6.059 ± 0.019
6.278 ± 0.019
1.445 ± 0.009
2.728 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here