Yoonia tamlensis
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3187 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6HAJ4|A0A1I6HAJ4_9RHOB Putative peptidoglycan binding domain-containing protein OS=Yoonia tamlensis OX=390270 GN=SAMN04488005_2429 PE=4 SV=1
MM1 pKa = 7.2 QFLNIRR7 pKa = 11.84 SGALAVAMLGAAAPAVAQDD26 pKa = 4.48 TYY28 pKa = 11.49 CGGAGNGGQWIGGDD42 pKa = 3.62 AQSSDD47 pKa = 2.79 IATAGSYY54 pKa = 9.04 QEE56 pKa = 4.45 QMALVLGGNEE66 pKa = 4.21 YY67 pKa = 10.4 ISLFSLSQGTDD78 pKa = 3.04 VRR80 pKa = 11.84 IEE82 pKa = 3.79 AAGRR86 pKa = 11.84 GAGDD90 pKa = 3.85 TIIDD94 pKa = 3.83 VLDD97 pKa = 3.98 SNGSIVASDD106 pKa = 4.27 DD107 pKa = 3.88 DD108 pKa = 4.38 SGGNAASRR116 pKa = 11.84 AEE118 pKa = 4.3 TYY120 pKa = 10.87 LEE122 pKa = 4.11 AGTYY126 pKa = 10.45 CMALRR131 pKa = 11.84 SYY133 pKa = 11.27 DD134 pKa = 4.11 GGPMTAFVRR143 pKa = 11.84 VGRR146 pKa = 11.84 QEE148 pKa = 4.69 HH149 pKa = 5.78 EE150 pKa = 4.01 ALTEE154 pKa = 4.02 GVMDD158 pKa = 4.12 SPVNDD163 pKa = 4.32 GSAGGCATATPMGGLGSSVTGSVADD188 pKa = 3.66 NPNWSFTLDD197 pKa = 3.25 APAQISITAEE207 pKa = 3.89 NEE209 pKa = 3.83 SADD212 pKa = 3.85 PVVTLYY218 pKa = 10.79 GANDD222 pKa = 3.36 MFIAEE227 pKa = 4.23 NDD229 pKa = 4.02 DD230 pKa = 3.72 YY231 pKa = 11.92 DD232 pKa = 4.15 GLNSRR237 pKa = 11.84 IDD239 pKa = 3.48 VTDD242 pKa = 3.27 TLDD245 pKa = 3.72 PGTYY249 pKa = 9.25 CIAMAALSDD258 pKa = 4.07 EE259 pKa = 4.45 YY260 pKa = 11.76 APITVSLTEE269 pKa = 4.28 YY270 pKa = 10.69 DD271 pKa = 3.81 PEE273 pKa = 5.26 AATLSLYY280 pKa = 10.76 DD281 pKa = 4.05 RR282 pKa = 11.84 GEE284 pKa = 4.05 AAPPLDD290 pKa = 3.38 GSIPVTDD297 pKa = 4.46 LGVLEE302 pKa = 4.28 SRR304 pKa = 11.84 VRR306 pKa = 11.84 QDD308 pKa = 2.85 VQATDD313 pKa = 3.34 DD314 pKa = 4.05 VTWFTLEE321 pKa = 3.99 VLDD324 pKa = 5.02 NSLLVIEE331 pKa = 5.58 AIGGGNSDD339 pKa = 3.6 PWLVVYY345 pKa = 10.62 DD346 pKa = 3.91 DD347 pKa = 4.61 LGRR350 pKa = 11.84 EE351 pKa = 3.83 VGMNDD356 pKa = 4.42 DD357 pKa = 4.47 YY358 pKa = 11.99 GDD360 pKa = 4.2 GLDD363 pKa = 3.81 SLVMARR369 pKa = 11.84 VQSGTYY375 pKa = 9.44 IVGVRR380 pKa = 11.84 QFDD383 pKa = 3.6 NTAGLIRR390 pKa = 11.84 LLAEE394 pKa = 4.12 RR395 pKa = 11.84 YY396 pKa = 9.25 VRR398 pKa = 11.84 AQQ400 pKa = 2.93
Molecular weight: 41.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.617
IPC_protein 3.656
Toseland 3.427
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.037
Thurlkill 3.478
EMBOSS 3.617
Sillero 3.783
Patrickios 0.973
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A1I6FQ09|A0A1I6FQ09_9RHOB Nucleobase:cation symporter-2 NCS2 family OS=Yoonia tamlensis OX=390270 GN=SAMN04488005_0233 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.53 ILNNRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.47 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3187
0
3187
974354
31
2420
305.7
33.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.616 ± 0.066
0.933 ± 0.012
6.339 ± 0.03
5.022 ± 0.042
3.856 ± 0.031
8.434 ± 0.04
2.074 ± 0.025
5.76 ± 0.033
3.32 ± 0.039
9.665 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.868 ± 0.023
2.946 ± 0.023
4.86 ± 0.03
3.554 ± 0.024
6.003 ± 0.041
5.001 ± 0.031
5.813 ± 0.036
7.257 ± 0.035
1.344 ± 0.016
2.336 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here