Corynebacterium flavescens
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2152 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L7CNB0|A0A1L7CNB0_CORFL ComEC/Rec2 family competence protein OS=Corynebacterium flavescens OX=28028 GN=CFLV_09180 PE=4 SV=1
MM1 pKa = 7.13 SQLPADD7 pKa = 3.66 YY8 pKa = 10.32 SYY10 pKa = 11.78 SEE12 pKa = 3.92 DD13 pKa = 4.2 HH14 pKa = 6.29 EE15 pKa = 5.0 WINAPADD22 pKa = 3.73 DD23 pKa = 4.19 VAGKK27 pKa = 6.84 TVRR30 pKa = 11.84 VGITSVAADD39 pKa = 3.73 RR40 pKa = 11.84 LGEE43 pKa = 4.25 VVFAEE48 pKa = 4.68 LPAVGDD54 pKa = 3.63 AVTAGEE60 pKa = 4.24 SCGEE64 pKa = 4.14 VEE66 pKa = 4.43 STKK69 pKa = 10.7 SVSDD73 pKa = 4.05 LYY75 pKa = 11.59 SPVTGTVTAINEE87 pKa = 4.68 AIGEE91 pKa = 4.36 DD92 pKa = 3.74 YY93 pKa = 11.42 SLINSDD99 pKa = 3.79 PFGEE103 pKa = 4.37 GWLWEE108 pKa = 4.28 VEE110 pKa = 4.24 VTEE113 pKa = 5.64 AGDD116 pKa = 3.6 LMSAEE121 pKa = 5.09 HH122 pKa = 6.35 YY123 pKa = 10.4 SAANGVV129 pKa = 3.3
Molecular weight: 13.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.745
IPC_protein 3.681
Toseland 3.49
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.567
Rodwell 3.516
Grimsley 3.414
Solomon 3.643
Lehninger 3.592
Nozaki 3.783
DTASelect 3.935
Thurlkill 3.541
EMBOSS 3.579
Sillero 3.795
Patrickios 0.846
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A1L7CQ56|A0A1L7CQ56_CORFL Chromosome partitioning protein OS=Corynebacterium flavescens OX=28028 GN=CFLV_12860 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.59 HH19 pKa = 4.58 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MSTRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.72 KK40 pKa = 10.5 GRR42 pKa = 11.84 AKK44 pKa = 9.67 LTAA47 pKa = 4.21
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2152
0
2152
708000
40
3019
329.0
35.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.008 ± 0.068
0.729 ± 0.014
5.775 ± 0.04
6.459 ± 0.052
3.393 ± 0.034
8.335 ± 0.051
2.177 ± 0.025
5.115 ± 0.036
3.288 ± 0.039
9.778 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.139 ± 0.023
2.777 ± 0.027
4.952 ± 0.035
3.409 ± 0.034
6.255 ± 0.049
6.205 ± 0.042
5.611 ± 0.038
7.961 ± 0.045
1.375 ± 0.019
2.259 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here