Moraxella phage Mcat17
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PIH3|A0A0R6PIH3_9CAUD Uncharacterized protein OS=Moraxella phage Mcat17 OX=1647532 PE=4 SV=1
MM1 pKa = 7.39 IASKK5 pKa = 10.57 RR6 pKa = 11.84 IFGLLGEE13 pKa = 4.56 LVNDD17 pKa = 3.65 RR18 pKa = 11.84 VYY20 pKa = 11.12 PLFVPEE26 pKa = 4.74 TADD29 pKa = 3.26 STPPFMVYY37 pKa = 9.4 TLVSNVPDD45 pKa = 3.65 NTLDD49 pKa = 3.51 GATGHH54 pKa = 6.12 EE55 pKa = 4.34 WVGVQIDD62 pKa = 4.68 IYY64 pKa = 11.07 AADD67 pKa = 4.0 YY68 pKa = 11.55 DD69 pKa = 4.13 EE70 pKa = 5.35 TIALAQEE77 pKa = 3.92 AVKK80 pKa = 10.44 RR81 pKa = 11.84 LNTIKK86 pKa = 10.09 PSEE89 pKa = 4.32 YY90 pKa = 9.31 GGVVYY95 pKa = 10.9 VHH97 pKa = 7.72 DD98 pKa = 5.59 DD99 pKa = 3.19 GLYY102 pKa = 9.37 RR103 pKa = 11.84 AIIEE107 pKa = 4.2 YY108 pKa = 9.78 EE109 pKa = 4.07 FWQTIII115 pKa = 4.15
Molecular weight: 12.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.96
IPC2_protein 4.266
IPC_protein 4.177
Toseland 3.999
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.062
Rodwell 4.012
Grimsley 3.91
Solomon 4.139
Lehninger 4.101
Nozaki 4.266
DTASelect 4.457
Thurlkill 4.037
EMBOSS 4.075
Sillero 4.291
Patrickios 1.977
IPC_peptide 4.139
IPC2_peptide 4.279
IPC2.peptide.svr19 4.18
Protein with the highest isoelectric point:
>tr|A0A0R6PDV7|A0A0R6PDV7_9CAUD Uncharacterized protein OS=Moraxella phage Mcat17 OX=1647532 PE=4 SV=1
MM1 pKa = 7.47 NKK3 pKa = 10.35 LILLLILVASQAQAHH18 pKa = 5.2 TATWYY23 pKa = 10.21 GGKK26 pKa = 9.55 FHH28 pKa = 6.99 GRR30 pKa = 11.84 LTASGEE36 pKa = 4.21 RR37 pKa = 11.84 YY38 pKa = 8.89 NQNALTCASNKK49 pKa = 10.06 YY50 pKa = 10.5 KK51 pKa = 10.66 FGTRR55 pKa = 11.84 LKK57 pKa = 9.5 VTNTANGKK65 pKa = 8.89 SVICRR70 pKa = 11.84 VNDD73 pKa = 3.4 RR74 pKa = 11.84 GGFGKK79 pKa = 10.83 YY80 pKa = 7.96 GTTLDD85 pKa = 3.77 LSKK88 pKa = 11.28 GAFKK92 pKa = 10.75 KK93 pKa = 10.06 IAPLSQGKK101 pKa = 9.66 IKK103 pKa = 9.86 VTIRR107 pKa = 11.84 RR108 pKa = 11.84 LL109 pKa = 3.28
Molecular weight: 11.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.292
IPC2_protein 10.014
IPC_protein 10.482
Toseland 10.877
ProMoST 10.438
Dawson 10.965
Bjellqvist 10.599
Wikipedia 11.111
Rodwell 11.462
Grimsley 10.994
Solomon 11.023
Lehninger 11.008
Nozaki 10.847
DTASelect 10.599
Thurlkill 10.862
EMBOSS 11.257
Sillero 10.877
Patrickios 11.199
IPC_peptide 11.038
IPC2_peptide 9.428
IPC2.peptide.svr19 8.375
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
12720
42
1944
249.4
27.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.569 ± 0.496
0.998 ± 0.236
6.069 ± 0.272
5.975 ± 0.365
3.451 ± 0.198
6.761 ± 0.327
1.965 ± 0.219
6.918 ± 0.282
7.146 ± 0.538
8.498 ± 0.327
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.524 ± 0.255
5.197 ± 0.451
3.2 ± 0.227
4.583 ± 0.221
4.788 ± 0.28
6.541 ± 0.497
6.219 ± 0.585
6.038 ± 0.197
1.313 ± 0.139
3.247 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here