Vibrio phage ValSw4_1
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411BJV6|A0A411BJV6_9CAUD Uncharacterized protein OS=Vibrio phage ValSw4_1 OX=2484246 GN=ValSw41_84 PE=4 SV=1
MM1 pKa = 7.38 EE2 pKa = 5.0 NVVNTLVVDD11 pKa = 4.28 FTNPSAISDD20 pKa = 3.87 GAILMLEE27 pKa = 3.93 YY28 pKa = 10.47 DD29 pKa = 3.82 ARR31 pKa = 11.84 EE32 pKa = 4.14 AADD35 pKa = 3.57 GGLNARR41 pKa = 11.84 STPYY45 pKa = 10.78 LNEE48 pKa = 3.64 SCYY51 pKa = 11.06 LLLSGYY57 pKa = 10.89 GLTNFNITASSGLAVFNSGVKK78 pKa = 9.32 YY79 pKa = 10.15 RR80 pKa = 11.84 QVTEE84 pKa = 3.86 TVIFDD89 pKa = 3.87 GSDD92 pKa = 3.18 TGSLRR97 pKa = 11.84 LPATRR102 pKa = 11.84 LISYY106 pKa = 8.58 SWQGSDD112 pKa = 4.89 GGALSLDD119 pKa = 3.75 DD120 pKa = 3.56 QVLRR124 pKa = 11.84 ISNEE128 pKa = 3.66 GNFVVSVTYY137 pKa = 10.1 EE138 pKa = 3.58 SSYY141 pKa = 11.63 YY142 pKa = 9.91 EE143 pKa = 4.03 IKK145 pKa = 9.86 LTPPEE150 pKa = 4.88 LDD152 pKa = 3.68 PDD154 pKa = 4.16 TVDD157 pKa = 3.4 EE158 pKa = 4.21 YY159 pKa = 10.58 TINVIVSAEE168 pKa = 4.13 VIQQ171 pKa = 4.16
Molecular weight: 18.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.884
IPC_protein 3.834
Toseland 3.643
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.139
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.961
Patrickios 0.655
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A411BJU0|A0A411BJU0_9CAUD Uncharacterized protein OS=Vibrio phage ValSw4_1 OX=2484246 GN=ValSw41_69 PE=4 SV=1
MM1 pKa = 7.34 AVKK4 pKa = 10.5 DD5 pKa = 4.31 KK6 pKa = 10.05 FSKK9 pKa = 10.23 MILRR13 pKa = 11.84 NGRR16 pKa = 11.84 VFHH19 pKa = 7.02 IAVTHH24 pKa = 6.37 DD25 pKa = 3.38 NRR27 pKa = 11.84 DD28 pKa = 3.38 VEE30 pKa = 4.38 TLIQVRR36 pKa = 11.84 DD37 pKa = 3.72 QNNEE41 pKa = 3.62 LLATAKK47 pKa = 10.06 VCRR50 pKa = 11.84 LFYY53 pKa = 9.78 FTFEE57 pKa = 3.81 IYY59 pKa = 10.51 RR60 pKa = 11.84 NGKK63 pKa = 5.25 TNRR66 pKa = 11.84 GKK68 pKa = 10.52 RR69 pKa = 11.84 RR70 pKa = 11.84 LSKK73 pKa = 9.0 FTPDD77 pKa = 3.19 KK78 pKa = 10.3 EE79 pKa = 3.9 PRR81 pKa = 11.84 YY82 pKa = 10.15 YY83 pKa = 9.75 STPVAAWLVDD93 pKa = 3.17 HH94 pKa = 7.12 HH95 pKa = 6.88 APKK98 pKa = 10.76 LKK100 pKa = 10.68
Molecular weight: 11.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 9.502
IPC_protein 9.633
Toseland 10.35
ProMoST 9.999
Dawson 10.482
Bjellqvist 10.116
Wikipedia 10.628
Rodwell 10.965
Grimsley 10.526
Solomon 10.526
Lehninger 10.496
Nozaki 10.321
DTASelect 10.116
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.394
Patrickios 10.701
IPC_peptide 10.526
IPC2_peptide 8.741
IPC2.peptide.svr19 8.563
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
107
0
107
23644
58
1379
221.0
24.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.061 ± 0.358
1.155 ± 0.119
6.441 ± 0.151
6.881 ± 0.233
3.667 ± 0.131
6.099 ± 0.169
2.017 ± 0.15
6.099 ± 0.223
6.75 ± 0.319
9.165 ± 0.218
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.318 ± 0.098
5.134 ± 0.177
3.87 ± 0.16
3.502 ± 0.175
5.376 ± 0.201
6.247 ± 0.215
6.213 ± 0.124
6.492 ± 0.223
0.791 ± 0.078
3.722 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here