Flavobacterium cellulosilyticum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3677 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5CJ79|A0A4R5CJ79_9FLAO Rrf2 family transcriptional regulator OS=Flavobacterium cellulosilyticum OX=2541731 GN=E0F76_00225 PE=4 SV=1
MM1 pKa = 7.05GQSSYY6 pKa = 10.92SQEE9 pKa = 3.62GWDD12 pKa = 5.41NEE14 pKa = 4.32EE15 pKa = 3.25EE16 pKa = 4.39WYY18 pKa = 10.45EE19 pKa = 4.06NEE21 pKa = 4.56SWDD24 pKa = 5.28SNDD27 pKa = 4.48NDD29 pKa = 4.31YY30 pKa = 10.94TNNWQDD36 pKa = 4.85FLSEE40 pKa = 4.06WFGLDD45 pKa = 2.71IAGITDD51 pKa = 4.07GGNFLLSNGDD61 pKa = 3.32YY62 pKa = 10.53FYY64 pKa = 10.62PDD66 pKa = 3.31SSNLDD71 pKa = 3.53EE72 pKa = 5.0VNVNSYY78 pKa = 10.11HH79 pKa = 7.6VSEE82 pKa = 4.29QYY84 pKa = 10.63EE85 pKa = 4.06YY86 pKa = 11.78VPIGEE91 pKa = 4.49NNGNPGGDD99 pKa = 3.34EE100 pKa = 4.16SEE102 pKa = 4.39EE103 pKa = 4.34TVDD106 pKa = 6.2DD107 pKa = 4.06PTDD110 pKa = 3.47NCSISYY116 pKa = 9.67CMPGYY121 pKa = 8.62TLQNCEE127 pKa = 4.36CVKK130 pKa = 10.44VSCTKK135 pKa = 9.85TCEE138 pKa = 3.82SGYY141 pKa = 10.75KK142 pKa = 10.47LNIDD146 pKa = 3.53TCEE149 pKa = 4.46CEE151 pKa = 5.0LLPPCWGTIQDD162 pKa = 4.41FEE164 pKa = 4.68TSNSFNTNTILNKK177 pKa = 10.39LNSTLGDD184 pKa = 3.58FGISADD190 pKa = 3.64VMDD193 pKa = 6.03SIKK196 pKa = 10.75QMNLFDD202 pKa = 5.24PKK204 pKa = 10.45TIATGTDD211 pKa = 3.24LVTFADD217 pKa = 4.04DD218 pKa = 4.1LGVVGDD224 pKa = 4.37GLQVALDD231 pKa = 3.53YY232 pKa = 11.27SKK234 pKa = 11.59YY235 pKa = 9.59IDD237 pKa = 4.05EE238 pKa = 5.4PSTQNLLRR246 pKa = 11.84TVLDD250 pKa = 4.0AASMALSPAASLALSTIDD268 pKa = 3.7YY269 pKa = 10.61FKK271 pKa = 11.41DD272 pKa = 3.11GDD274 pKa = 4.25GNSKK278 pKa = 10.84LDD280 pKa = 4.28LLLKK284 pKa = 10.15AASDD288 pKa = 4.47AIDD291 pKa = 4.12RR292 pKa = 11.84SLDD295 pKa = 3.55CNLGLGIRR303 pKa = 11.84NWVYY307 pKa = 11.28

Molecular weight: 33.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5C6Y9|A0A4R5C6Y9_9FLAO Biopolymer transporter ExbD OS=Flavobacterium cellulosilyticum OX=2541731 GN=E0F76_13895 PE=3 SV=1
KKK2 pKa = 10.22FNTISRR8 pKa = 11.84TIINHHH14 pKa = 4.91YY15 pKa = 7.47TILNYYY21 pKa = 9.99DDD23 pKa = 4.54RR24 pKa = 11.84STNASAEEE32 pKa = 4.19FNAKKK37 pKa = 9.25KKK39 pKa = 10.63FRR41 pKa = 11.84SQFRR45 pKa = 11.84GVRR48 pKa = 11.84NIEEE52 pKa = 3.78FLFRR56 pKa = 11.84LTNIYYY62 pKa = 10.64

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3677

0

3677

1329845

27

4425

361.7

40.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.336 ± 0.037

0.795 ± 0.013

5.215 ± 0.028

6.085 ± 0.049

5.243 ± 0.037

6.441 ± 0.038

1.627 ± 0.017

8.454 ± 0.042

7.977 ± 0.053

9.047 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.213 ± 0.024

6.655 ± 0.043

3.449 ± 0.024

3.341 ± 0.025

3.031 ± 0.025

6.78 ± 0.042

6.046 ± 0.078

6.056 ± 0.032

1.124 ± 0.016

4.087 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski