Amino acid dipepetide frequency for Flavobacterium cellulosilyticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.349AlaAla: 4.349 ± 0.085	0.508AlaCys: 0.508 ± 0.024	3.146AlaAsp: 3.146 ± 0.061	3.528AlaGlu: 3.528 ± 0.06	3.23AlaPhe: 3.23 ± 0.054	4.249AlaGly: 4.249 ± 0.084	0.926AlaHis: 0.926 ± 0.029	5.773AlaIle: 5.773 ± 0.072	4.812AlaLys: 4.812 ± 0.091	5.875AlaLeu: 5.875 ± 0.08	1.545AlaMet: 1.545 ± 0.039	3.918AlaAsn: 3.918 ± 0.066	1.933AlaPro: 1.933 ± 0.06	2.307AlaGln: 2.307 ± 0.043	1.762AlaArg: 1.762 ± 0.038	4.296AlaSer: 4.296 ± 0.088	4.229AlaThr: 4.229 ± 0.105	4.08AlaVal: 4.08 ± 0.064	0.626AlaTrp: 0.626 ± 0.028	2.264AlaTyr: 2.264 ± 0.045	0.002AlaXaa: 0.002 ± 0.001
Cys	0.467CysAla: 0.467 ± 0.024	0.106CysCys: 0.106 ± 0.009	0.436CysAsp: 0.436 ± 0.026	0.456CysGlu: 0.456 ± 0.023	0.456CysPhe: 0.456 ± 0.02	0.613CysGly: 0.613 ± 0.022	0.189CysHis: 0.189 ± 0.015	0.647CysIle: 0.647 ± 0.023	0.502CysLys: 0.502 ± 0.021	0.65CysLeu: 0.65 ± 0.025	0.146CysMet: 0.146 ± 0.011	0.448CysAsn: 0.448 ± 0.016	0.361CysPro: 0.361 ± 0.021	0.234CysGln: 0.234 ± 0.014	0.203CysArg: 0.203 ± 0.013	0.662CysSer: 0.662 ± 0.026	0.519CysThr: 0.519 ± 0.026	0.455CysVal: 0.455 ± 0.018	0.081CysTrp: 0.081 ± 0.008	0.316CysTyr: 0.316 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.479AspAla: 3.479 ± 0.062	0.428AspCys: 0.428 ± 0.02	2.406AspAsp: 2.406 ± 0.046	3.274AspGlu: 3.274 ± 0.055	3.546AspPhe: 3.546 ± 0.06	3.54AspGly: 3.54 ± 0.067	0.775AspHis: 0.775 ± 0.029	4.159AspIle: 4.159 ± 0.062	4.294AspLys: 4.294 ± 0.072	5.029AspLeu: 5.029 ± 0.07	1.029AspMet: 1.029 ± 0.031	3.121AspAsn: 3.121 ± 0.052	1.644AspPro: 1.644 ± 0.037	1.445AspGln: 1.445 ± 0.029	1.647AspArg: 1.647 ± 0.032	3.09AspSer: 3.09 ± 0.053	2.535AspThr: 2.535 ± 0.04	3.284AspVal: 3.284 ± 0.051	0.735AspTrp: 0.735 ± 0.025	2.688AspTyr: 2.688 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	3.872GluAla: 3.872 ± 0.076	0.391GluCys: 0.391 ± 0.019	2.903GluAsp: 2.903 ± 0.052	3.953GluGlu: 3.953 ± 0.085	2.819GluPhe: 2.819 ± 0.048	3.38GluGly: 3.38 ± 0.054	0.953GluHis: 0.953 ± 0.03	5.67GluIle: 5.67 ± 0.081	5.902GluLys: 5.902 ± 0.089	5.687GluLeu: 5.687 ± 0.089	1.585GluMet: 1.585 ± 0.036	4.722GluAsn: 4.722 ± 0.07	1.381GluPro: 1.381 ± 0.036	1.99GluGln: 1.99 ± 0.048	2.034GluArg: 2.034 ± 0.043	3.409GluSer: 3.409 ± 0.059	3.429GluThr: 3.429 ± 0.053	3.847GluVal: 3.847 ± 0.059	0.603GluTrp: 0.603 ± 0.019	2.315GluTyr: 2.315 ± 0.044	0.002GluXaa: 0.002 ± 0.001
Phe	3.048PheAla: 3.048 ± 0.05	0.444PheCys: 0.444 ± 0.018	3.287PheAsp: 3.287 ± 0.055	3.41PheGlu: 3.41 ± 0.054	2.702PhePhe: 2.702 ± 0.061	3.677PheGly: 3.677 ± 0.062	0.802PheHis: 0.802 ± 0.026	4.092PheIle: 4.092 ± 0.068	3.749PheLys: 3.749 ± 0.06	4.584PheLeu: 4.584 ± 0.072	1.151PheMet: 1.151 ± 0.029	3.357PheAsn: 3.357 ± 0.057	1.848PhePro: 1.848 ± 0.037	1.623PheGln: 1.623 ± 0.037	1.536PheArg: 1.536 ± 0.038	4.14PheSer: 4.14 ± 0.066	3.13PheThr: 3.13 ± 0.059	3.118PheVal: 3.118 ± 0.057	0.629PheTrp: 0.629 ± 0.023	2.101PheTyr: 2.101 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.089GlyAla: 4.089 ± 0.087	0.657GlyCys: 0.657 ± 0.029	3.13GlyAsp: 3.13 ± 0.067	3.257GlyGlu: 3.257 ± 0.055	3.591GlyPhe: 3.591 ± 0.058	4.498GlyGly: 4.498 ± 0.086	1.111GlyHis: 1.111 ± 0.028	5.847GlyIle: 5.847 ± 0.069	5.134GlyLys: 5.134 ± 0.067	5.719GlyLeu: 5.719 ± 0.065	1.656GlyMet: 1.656 ± 0.037	4.04GlyAsn: 4.04 ± 0.078	1.355GlyPro: 1.355 ± 0.039	1.88GlyGln: 1.88 ± 0.037	1.909GlyArg: 1.909 ± 0.038	4.242GlySer: 4.242 ± 0.091	4.41GlyThr: 4.41 ± 0.107	4.237GlyVal: 4.237 ± 0.079	0.815GlyTrp: 0.815 ± 0.028	2.831GlyTyr: 2.831 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	0.855HisAla: 0.855 ± 0.028	0.177HisCys: 0.177 ± 0.013	0.766HisAsp: 0.766 ± 0.024	0.857HisGlu: 0.857 ± 0.029	1.145HisPhe: 1.145 ± 0.03	0.987HisGly: 0.987 ± 0.031	0.46HisHis: 0.46 ± 0.019	1.325HisIle: 1.325 ± 0.029	1.086HisLys: 1.086 ± 0.033	1.662HisLeu: 1.662 ± 0.041	0.271HisMet: 0.271 ± 0.014	0.979HisAsn: 0.979 ± 0.028	0.815HisPro: 0.815 ± 0.024	0.682HisGln: 0.682 ± 0.025	0.539HisArg: 0.539 ± 0.023	1.06HisSer: 1.06 ± 0.028	0.834HisThr: 0.834 ± 0.026	0.769HisVal: 0.769 ± 0.026	0.218HisTrp: 0.218 ± 0.013	0.781HisTyr: 0.781 ± 0.024	0.001HisXaa: 0.001 ± 0.001
Ile	5.983IleAla: 5.983 ± 0.079	0.744IleCys: 0.744 ± 0.029	4.897IleAsp: 4.897 ± 0.063	5.551IleGlu: 5.551 ± 0.078	3.637IlePhe: 3.637 ± 0.069	5.51IleGly: 5.51 ± 0.079	1.402IleHis: 1.402 ± 0.038	6.896IleIle: 6.896 ± 0.1	6.353IleLys: 6.353 ± 0.07	7.32IleLeu: 7.32 ± 0.085	1.481IleMet: 1.481 ± 0.04	5.413IleAsn: 5.413 ± 0.078	3.533IlePro: 3.533 ± 0.054	2.915IleGln: 2.915 ± 0.053	2.575IleArg: 2.575 ± 0.046	6.077IleSer: 6.077 ± 0.071	5.271IleThr: 5.271 ± 0.079	5.197IleVal: 5.197 ± 0.081	0.795IleTrp: 0.795 ± 0.025	2.986IleTyr: 2.986 ± 0.049	0.002IleXaa: 0.002 ± 0.001
Lys	4.825LysAla: 4.825 ± 0.087	0.39LysCys: 0.39 ± 0.017	4.189LysAsp: 4.189 ± 0.068	5.915LysGlu: 5.915 ± 0.095	3.067LysPhe: 3.067 ± 0.053	4.773LysGly: 4.773 ± 0.069	1.176LysHis: 1.176 ± 0.034	7.252LysIle: 7.252 ± 0.088	6.901LysLys: 6.901 ± 0.104	6.679LysLeu: 6.679 ± 0.088	2.298LysMet: 2.298 ± 0.045	5.962LysAsn: 5.962 ± 0.078	2.523LysPro: 2.523 ± 0.048	2.679LysGln: 2.679 ± 0.053	2.428LysArg: 2.428 ± 0.047	4.974LysSer: 4.974 ± 0.068	4.95LysThr: 4.95 ± 0.068	4.728LysVal: 4.728 ± 0.072	0.871LysTrp: 0.871 ± 0.026	3.188LysTyr: 3.188 ± 0.053	0.0LysXaa: 0.0 ± 0.0
Leu	5.731LeuAla: 5.731 ± 0.071	0.702LeuCys: 0.702 ± 0.023	4.812LeuAsp: 4.812 ± 0.069	5.585LeuGlu: 5.585 ± 0.085	5.058LeuPhe: 5.058 ± 0.075	5.725LeuGly: 5.725 ± 0.07	1.499LeuHis: 1.499 ± 0.04	7.046LeuIle: 7.046 ± 0.087	7.572LeuLys: 7.572 ± 0.102	8.537LeuLeu: 8.537 ± 0.118	2.09LeuMet: 2.09 ± 0.047	5.841LeuAsn: 5.841 ± 0.081	3.546LeuPro: 3.546 ± 0.055	3.014LeuGln: 3.014 ± 0.046	2.768LeuArg: 2.768 ± 0.05	6.552LeuSer: 6.552 ± 0.077	5.104LeuThr: 5.104 ± 0.072	5.143LeuVal: 5.143 ± 0.06	0.882LeuTrp: 0.882 ± 0.035	3.258LeuTyr: 3.258 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	1.496MetAla: 1.496 ± 0.029	0.145MetCys: 0.145 ± 0.011	1.118MetAsp: 1.118 ± 0.033	1.367MetGlu: 1.367 ± 0.039	0.842MetPhe: 0.842 ± 0.029	1.436MetGly: 1.436 ± 0.035	0.394MetHis: 0.394 ± 0.016	1.669MetIle: 1.669 ± 0.043	2.351MetLys: 2.351 ± 0.044	1.836MetLeu: 1.836 ± 0.042	0.684MetMet: 0.684 ± 0.027	1.491MetAsn: 1.491 ± 0.033	0.854MetPro: 0.854 ± 0.023	0.807MetGln: 0.807 ± 0.027	0.789MetArg: 0.789 ± 0.028	1.41MetSer: 1.41 ± 0.034	1.224MetThr: 1.224 ± 0.036	1.32MetVal: 1.32 ± 0.035	0.191MetTrp: 0.191 ± 0.013	0.709MetTyr: 0.709 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	4.052AsnAla: 4.052 ± 0.065	0.578AsnCys: 0.578 ± 0.029	3.357AsnAsp: 3.357 ± 0.063	3.925AsnGlu: 3.925 ± 0.063	3.267AsnPhe: 3.267 ± 0.054	4.507AsnGly: 4.507 ± 0.091	1.12AsnHis: 1.12 ± 0.034	5.028AsnIle: 5.028 ± 0.061	5.023AsnLys: 5.023 ± 0.068	5.738AsnLeu: 5.738 ± 0.074	1.23AsnMet: 1.23 ± 0.031	4.488AsnAsn: 4.488 ± 0.08	3.159AsnPro: 3.159 ± 0.058	2.552AsnGln: 2.552 ± 0.052	2.023AsnArg: 2.023 ± 0.049	4.646AsnSer: 4.646 ± 0.075	3.967AsnThr: 3.967 ± 0.07	3.67AsnVal: 3.67 ± 0.064	0.944AsnTrp: 0.944 ± 0.028	3.273AsnTyr: 3.273 ± 0.064	0.001AsnXaa: 0.001 ± 0.001
Pro	2.097ProAla: 2.097 ± 0.049	0.21ProCys: 0.21 ± 0.016	1.878ProAsp: 1.878 ± 0.038	2.628ProGlu: 2.628 ± 0.041	1.984ProPhe: 1.984 ± 0.04	1.955ProGly: 1.955 ± 0.044	0.52ProHis: 0.52 ± 0.019	3.07ProIle: 3.07 ± 0.046	2.64ProLys: 2.64 ± 0.05	2.97ProLeu: 2.97 ± 0.058	0.77ProMet: 0.77 ± 0.024	2.471ProAsn: 2.471 ± 0.044	0.766ProPro: 0.766 ± 0.027	1.08ProGln: 1.08 ± 0.03	0.872ProArg: 0.872 ± 0.027	2.381ProSer: 2.381 ± 0.052	2.198ProThr: 2.198 ± 0.063	2.262ProVal: 2.262 ± 0.045	0.376ProTrp: 0.376 ± 0.017	1.359ProTyr: 1.359 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	1.899GlnAla: 1.899 ± 0.048	0.219GlnCys: 0.219 ± 0.018	1.568GlnAsp: 1.568 ± 0.038	2.149GlnGlu: 2.149 ± 0.04	1.787GlnPhe: 1.787 ± 0.038	1.736GlnGly: 1.736 ± 0.039	0.541GlnHis: 0.541 ± 0.023	2.876GlnIle: 2.876 ± 0.048	3.104GlnLys: 3.104 ± 0.061	3.352GlnLeu: 3.352 ± 0.056	0.804GlnMet: 0.804 ± 0.026	2.455GlnAsn: 2.455 ± 0.049	0.984GlnPro: 0.984 ± 0.028	1.286GlnGln: 1.286 ± 0.035	1.058GlnArg: 1.058 ± 0.037	2.123GlnSer: 2.123 ± 0.04	1.963GlnThr: 1.963 ± 0.04	1.781GlnVal: 1.781 ± 0.032	0.386GlnTrp: 0.386 ± 0.02	1.334GlnTyr: 1.334 ± 0.033	0.001GlnXaa: 0.001 ± 0.001
Arg	1.846ArgAla: 1.846 ± 0.041	0.206ArgCys: 0.206 ± 0.014	1.511ArgAsp: 1.511 ± 0.037	1.828ArgGlu: 1.828 ± 0.039	1.683ArgPhe: 1.683 ± 0.035	1.687ArgGly: 1.687 ± 0.046	0.506ArgHis: 0.506 ± 0.019	2.721ArgIle: 2.721 ± 0.048	2.587ArgLys: 2.587 ± 0.054	2.835ArgLeu: 2.835 ± 0.049	0.811ArgMet: 0.811 ± 0.028	2.072ArgAsn: 2.072 ± 0.04	0.996ArgPro: 0.996 ± 0.028	0.901ArgGln: 0.901 ± 0.027	1.05ArgArg: 1.05 ± 0.037	1.799ArgSer: 1.799 ± 0.037	1.771ArgThr: 1.771 ± 0.04	1.811ArgVal: 1.811 ± 0.038	0.366ArgTrp: 0.366 ± 0.017	1.324ArgTyr: 1.324 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	3.925SerAla: 3.925 ± 0.061	0.684SerCys: 0.684 ± 0.024	3.449SerAsp: 3.449 ± 0.053	3.807SerGlu: 3.807 ± 0.069	3.989SerPhe: 3.989 ± 0.058	4.946SerGly: 4.946 ± 0.091	1.075SerHis: 1.075 ± 0.03	5.839SerIle: 5.839 ± 0.078	5.289SerLys: 5.289 ± 0.063	5.969SerLeu: 5.969 ± 0.062	1.272SerMet: 1.272 ± 0.031	4.554SerAsn: 4.554 ± 0.083	2.275SerPro: 2.275 ± 0.051	2.287SerGln: 2.287 ± 0.041	1.966SerArg: 1.966 ± 0.037	4.606SerSer: 4.606 ± 0.076	4.034SerThr: 4.034 ± 0.081	4.09SerVal: 4.09 ± 0.062	0.766SerTrp: 0.766 ± 0.027	2.98SerTyr: 2.98 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	4.182ThrAla: 4.182 ± 0.113	0.36ThrCys: 0.36 ± 0.022	3.163ThrAsp: 3.163 ± 0.054	3.122ThrGlu: 3.122 ± 0.049	3.144ThrPhe: 3.144 ± 0.057	4.102ThrGly: 4.102 ± 0.084	0.913ThrHis: 0.913 ± 0.028	5.9ThrIle: 5.9 ± 0.092	4.202ThrLys: 4.202 ± 0.063	5.404ThrLeu: 5.404 ± 0.072	1.026ThrMet: 1.026 ± 0.035	3.837ThrAsn: 3.837 ± 0.079	2.599ThrPro: 2.599 ± 0.06	1.93ThrGln: 1.93 ± 0.046	1.497ThrArg: 1.497 ± 0.035	4.088ThrSer: 4.088 ± 0.083	4.02ThrThr: 4.02 ± 0.114	3.937ThrVal: 3.937 ± 0.097	0.613ThrTrp: 0.613 ± 0.028	2.427ThrTyr: 2.427 ± 0.054	0.0ThrXaa: 0.0 ± 0.0
Val	4.161ValAla: 4.161 ± 0.08	0.538ValCys: 0.538 ± 0.02	3.241ValAsp: 3.241 ± 0.05	3.393ValGlu: 3.393 ± 0.062	3.421ValPhe: 3.421 ± 0.051	3.624ValGly: 3.624 ± 0.061	0.924ValHis: 0.924 ± 0.029	4.931ValIle: 4.931 ± 0.066	4.334ValLys: 4.334 ± 0.066	5.861ValLeu: 5.861 ± 0.073	1.246ValMet: 1.246 ± 0.037	3.751ValAsn: 3.751 ± 0.072	2.09ValPro: 2.09 ± 0.047	1.818ValGln: 1.818 ± 0.036	1.867ValArg: 1.867 ± 0.04	4.508ValSer: 4.508 ± 0.075	3.731ValThr: 3.731 ± 0.097	4.14ValVal: 4.14 ± 0.078	0.635ValTrp: 0.635 ± 0.022	2.345ValTyr: 2.345 ± 0.047	0.001ValXaa: 0.001 ± 0.001
Trp	0.662TrpAla: 0.662 ± 0.025	0.112TrpCys: 0.112 ± 0.009	0.669TrpAsp: 0.669 ± 0.023	0.693TrpGlu: 0.693 ± 0.024	0.566TrpPhe: 0.566 ± 0.023	0.751TrpGly: 0.751 ± 0.025	0.216TrpHis: 0.216 ± 0.011	0.829TrpIle: 0.829 ± 0.029	0.902TrpLys: 0.902 ± 0.029	0.908TrpLeu: 0.908 ± 0.025	0.314TrpMet: 0.314 ± 0.015	0.835TrpAsn: 0.835 ± 0.026	0.283TrpPro: 0.283 ± 0.016	0.437TrpGln: 0.437 ± 0.018	0.374TrpArg: 0.374 ± 0.019	0.78TrpSer: 0.78 ± 0.026	0.627TrpThr: 0.627 ± 0.022	0.629TrpVal: 0.629 ± 0.022	0.164TrpTrp: 0.164 ± 0.011	0.49TrpTyr: 0.49 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.34TyrAla: 2.34 ± 0.043	0.347TyrCys: 0.347 ± 0.018	2.224TyrAsp: 2.224 ± 0.039	2.147TyrGlu: 2.147 ± 0.044	2.494TyrPhe: 2.494 ± 0.054	2.709TyrGly: 2.709 ± 0.049	0.766TyrHis: 0.766 ± 0.022	2.97TyrIle: 2.97 ± 0.046	3.033TyrLys: 3.033 ± 0.061	3.852TyrLeu: 3.852 ± 0.06	0.722TyrMet: 0.722 ± 0.026	2.793TyrAsn: 2.793 ± 0.052	1.54TyrPro: 1.54 ± 0.036	1.539TyrGln: 1.539 ± 0.036	1.411TyrArg: 1.411 ± 0.039	2.957TyrSer: 2.957 ± 0.056	2.517TyrThr: 2.517 ± 0.066	2.062TyrVal: 2.062 ± 0.04	0.549TyrTrp: 0.549 ± 0.021	1.899TyrTyr: 1.899 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.002XaaLys: 0.002 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3677 proteins (1329845 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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