Mucilaginibacter ginsenosidivorans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4731 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8V0Y4|A0A5B8V0Y4_9SPHI 50S ribosomal protein L10 OS=Mucilaginibacter ginsenosidivorans OX=398053 GN=rplJ PE=3 SV=1
MM1 pKa = 7.47ANSYY5 pKa = 10.58GITVALDD12 pKa = 3.35SYY14 pKa = 11.26TLGKK18 pKa = 9.67LQAGNYY24 pKa = 8.52SLQVFKK30 pKa = 10.73GAKK33 pKa = 9.17GNQSTALPTLWFSVTEE49 pKa = 4.73FSATVSLKK57 pKa = 8.4WTEE60 pKa = 3.83QYY62 pKa = 11.41GAFVDD67 pKa = 3.86NQTIANGVVVDD78 pKa = 4.41ISSSKK83 pKa = 11.09AMNLGDD89 pKa = 5.64LMTLLSDD96 pKa = 3.7GDD98 pKa = 4.4TNVTTTGGRR107 pKa = 11.84DD108 pKa = 3.17GAITVFSQQSNEE120 pKa = 4.36WICGMTQSMNGSAPTPICAFPLFGSQADD148 pKa = 3.56LMEE151 pKa = 5.06PYY153 pKa = 8.78EE154 pKa = 4.39TVVLMFASGQVATGTVVEE172 pKa = 4.34EE173 pKa = 4.96AISSSVTITLSGAAPSVAVAFDD195 pKa = 4.31MNLGWNTSGNPYY207 pKa = 10.63AVVNPLPINLAEE219 pKa = 4.12TLIVPISS226 pKa = 3.35

Molecular weight: 23.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8URR7|A0A5B8URR7_9SPHI Type I pullulanase OS=Mucilaginibacter ginsenosidivorans OX=398053 GN=pulA PE=4 SV=1
MM1 pKa = 7.53LKK3 pKa = 9.7SCQSIYY9 pKa = 10.38VSLYY13 pKa = 8.82RR14 pKa = 11.84WTFKK18 pKa = 11.16NFGQGDD24 pKa = 3.67QPRR27 pKa = 11.84FKK29 pKa = 11.14SLFNVSFLLIIALTCLLLVMQLMLRR54 pKa = 11.84SQMITATPLTEE65 pKa = 3.92TTLVFGATFLLLINHH80 pKa = 6.15FMFLNNKK87 pKa = 6.5WFKK90 pKa = 10.92NINARR95 pKa = 11.84MRR97 pKa = 11.84LLGRR101 pKa = 11.84HH102 pKa = 5.71NPNTWSVVVLVNVILICGFLIFTIGG127 pKa = 3.02

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4731

0

4731

1570989

25

2948

332.1

37.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.695 ± 0.036

0.856 ± 0.011

5.633 ± 0.029

5.339 ± 0.039

4.884 ± 0.027

7.059 ± 0.036

2.013 ± 0.017

7.157 ± 0.029

6.902 ± 0.033

9.329 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.017

5.343 ± 0.036

3.988 ± 0.022

3.592 ± 0.02

4.057 ± 0.025

6.185 ± 0.032

5.8 ± 0.042

6.464 ± 0.03

1.21 ± 0.016

4.14 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski