Amino acid dipepetide frequency for Roseiconus nitratireducens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.951AlaAla: 11.951 ± 0.121	1.117AlaCys: 1.117 ± 0.031	6.775AlaAsp: 6.775 ± 0.066	6.694AlaGlu: 6.694 ± 0.07	3.351AlaPhe: 3.351 ± 0.047	8.277AlaGly: 8.277 ± 0.08	1.485AlaHis: 1.485 ± 0.029	5.405AlaIle: 5.405 ± 0.048	3.355AlaLys: 3.355 ± 0.048	8.402AlaLeu: 8.402 ± 0.082	2.619AlaMet: 2.619 ± 0.041	2.76AlaAsn: 2.76 ± 0.039	4.268AlaPro: 4.268 ± 0.053	3.099AlaGln: 3.099 ± 0.034	5.686AlaArg: 5.686 ± 0.058	6.808AlaSer: 6.808 ± 0.069	5.878AlaThr: 5.878 ± 0.07	7.306AlaVal: 7.306 ± 0.067	1.361AlaTrp: 1.361 ± 0.03	1.973AlaTyr: 1.973 ± 0.028	0.0AlaXaa: 0.0 ± 0.0
Cys	0.749CysAla: 0.749 ± 0.02	0.22CysCys: 0.22 ± 0.011	0.777CysAsp: 0.777 ± 0.022	0.663CysGlu: 0.663 ± 0.02	0.441CysPhe: 0.441 ± 0.014	1.077CysGly: 1.077 ± 0.028	0.47CysHis: 0.47 ± 0.02	0.406CysIle: 0.406 ± 0.013	0.262CysLys: 0.262 ± 0.013	1.138CysLeu: 1.138 ± 0.026	0.181CysMet: 0.181 ± 0.009	0.298CysAsn: 0.298 ± 0.012	0.585CysPro: 0.585 ± 0.02	0.463CysGln: 0.463 ± 0.017	0.857CysArg: 0.857 ± 0.022	0.628CysSer: 0.628 ± 0.018	0.475CysThr: 0.475 ± 0.015	0.829CysVal: 0.829 ± 0.022	0.193CysTrp: 0.193 ± 0.01	0.299CysTyr: 0.299 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.14AspAla: 7.14 ± 0.073	0.631AspCys: 0.631 ± 0.02	4.609AspAsp: 4.609 ± 0.068	3.968AspGlu: 3.968 ± 0.044	2.433AspPhe: 2.433 ± 0.037	5.996AspGly: 5.996 ± 0.094	1.606AspHis: 1.606 ± 0.032	2.123AspIle: 2.123 ± 0.04	1.437AspLys: 1.437 ± 0.025	6.368AspLeu: 6.368 ± 0.06	0.934AspMet: 0.934 ± 0.021	1.602AspAsn: 1.602 ± 0.04	4.554AspPro: 4.554 ± 0.053	3.514AspGln: 3.514 ± 0.045	5.414AspArg: 5.414 ± 0.05	4.199AspSer: 4.199 ± 0.059	2.602AspThr: 2.602 ± 0.053	4.638AspVal: 4.638 ± 0.056	1.134AspTrp: 1.134 ± 0.026	1.596AspTyr: 1.596 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	5.421GluAla: 5.421 ± 0.068	0.507GluCys: 0.507 ± 0.017	3.058GluAsp: 3.058 ± 0.036	3.0GluGlu: 3.0 ± 0.053	2.23GluPhe: 2.23 ± 0.034	3.735GluGly: 3.735 ± 0.042	1.378GluHis: 1.378 ± 0.026	3.374GluIle: 3.374 ± 0.041	2.139GluLys: 2.139 ± 0.04	6.515GluLeu: 6.515 ± 0.068	1.46GluMet: 1.46 ± 0.027	1.906GluAsn: 1.906 ± 0.029	2.941GluPro: 2.941 ± 0.041	3.125GluGln: 3.125 ± 0.048	4.21GluArg: 4.21 ± 0.056	4.866GluSer: 4.866 ± 0.053	3.782GluThr: 3.782 ± 0.035	4.027GluVal: 4.027 ± 0.046	0.678GluTrp: 0.678 ± 0.018	1.306GluTyr: 1.306 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.683PheAla: 3.683 ± 0.044	0.471PheCys: 0.471 ± 0.015	2.978PheAsp: 2.978 ± 0.043	2.184PheGlu: 2.184 ± 0.034	1.42PhePhe: 1.42 ± 0.03	3.318PheGly: 3.318 ± 0.04	0.787PheHis: 0.787 ± 0.02	1.303PheIle: 1.303 ± 0.026	0.843PheLys: 0.843 ± 0.02	3.429PheLeu: 3.429 ± 0.046	0.517PheMet: 0.517 ± 0.016	1.076PheAsn: 1.076 ± 0.026	1.647PhePro: 1.647 ± 0.03	1.478PheGln: 1.478 ± 0.026	2.557PheArg: 2.557 ± 0.032	2.344PheSer: 2.344 ± 0.035	1.801PheThr: 1.801 ± 0.039	2.718PheVal: 2.718 ± 0.039	0.551PheTrp: 0.551 ± 0.02	0.875PheTyr: 0.875 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	6.12GlyAla: 6.12 ± 0.077	1.014GlyCys: 1.014 ± 0.031	5.408GlyAsp: 5.408 ± 0.079	4.757GlyGlu: 4.757 ± 0.052	2.995GlyPhe: 2.995 ± 0.038	7.017GlyGly: 7.017 ± 0.108	1.715GlyHis: 1.715 ± 0.029	3.914GlyIle: 3.914 ± 0.045	3.164GlyLys: 3.164 ± 0.043	6.882GlyLeu: 6.882 ± 0.074	1.856GlyMet: 1.856 ± 0.034	2.713GlyAsn: 2.713 ± 0.062	3.509GlyPro: 3.509 ± 0.043	3.5GlyGln: 3.5 ± 0.044	5.697GlyArg: 5.697 ± 0.064	5.208GlySer: 5.208 ± 0.085	4.672GlyThr: 4.672 ± 0.076	5.422GlyVal: 5.422 ± 0.055	1.413GlyTrp: 1.413 ± 0.031	2.133GlyTyr: 2.133 ± 0.035	0.0GlyXaa: 0.0 ± 0.0
His	1.992HisAla: 1.992 ± 0.033	0.353HisCys: 0.353 ± 0.011	1.339HisAsp: 1.339 ± 0.031	1.154HisGlu: 1.154 ± 0.028	0.908HisPhe: 0.908 ± 0.021	1.775HisGly: 1.775 ± 0.033	0.717HisHis: 0.717 ± 0.02	0.68HisIle: 0.68 ± 0.02	0.414HisLys: 0.414 ± 0.013	2.319HisLeu: 2.319 ± 0.035	0.307HisMet: 0.307 ± 0.012	0.548HisAsn: 0.548 ± 0.016	1.545HisPro: 1.545 ± 0.029	1.024HisGln: 1.024 ± 0.028	1.933HisArg: 1.933 ± 0.032	1.242HisSer: 1.242 ± 0.022	0.891HisThr: 0.891 ± 0.022	1.558HisVal: 1.558 ± 0.028	0.471HisTrp: 0.471 ± 0.018	0.61HisTyr: 0.61 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	5.605IleAla: 5.605 ± 0.057	0.589IleCys: 0.589 ± 0.017	4.227IleAsp: 4.227 ± 0.053	3.664IleGlu: 3.664 ± 0.038	1.296IlePhe: 1.296 ± 0.026	4.181IleGly: 4.181 ± 0.05	1.033IleHis: 1.033 ± 0.023	1.615IleIle: 1.615 ± 0.029	1.252IleLys: 1.252 ± 0.026	3.8IleLeu: 3.8 ± 0.042	0.58IleMet: 0.58 ± 0.017	1.467IleAsn: 1.467 ± 0.031	2.365IlePro: 2.365 ± 0.034	1.777IleGln: 1.777 ± 0.03	3.671IleArg: 3.671 ± 0.043	2.771IleSer: 2.771 ± 0.041	2.432IleThr: 2.432 ± 0.046	3.654IleVal: 3.654 ± 0.044	0.593IleTrp: 0.593 ± 0.017	1.044IleTyr: 1.044 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	2.593LysAla: 2.593 ± 0.045	0.252LysCys: 0.252 ± 0.012	1.527LysAsp: 1.527 ± 0.031	1.601LysGlu: 1.601 ± 0.031	0.931LysPhe: 0.931 ± 0.019	1.696LysGly: 1.696 ± 0.032	0.733LysHis: 0.733 ± 0.017	1.467LysIle: 1.467 ± 0.024	1.227LysLys: 1.227 ± 0.034	3.248LysLeu: 3.248 ± 0.045	0.721LysMet: 0.721 ± 0.02	0.902LysAsn: 0.902 ± 0.022	1.931LysPro: 1.931 ± 0.035	1.502LysGln: 1.502 ± 0.032	2.429LysArg: 2.429 ± 0.044	2.052LysSer: 2.052 ± 0.038	1.82LysThr: 1.82 ± 0.029	2.084LysVal: 2.084 ± 0.036	0.438LysTrp: 0.438 ± 0.015	0.715LysTyr: 0.715 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	10.88LeuAla: 10.88 ± 0.086	1.104LeuCys: 1.104 ± 0.025	6.185LeuAsp: 6.185 ± 0.055	5.408LeuGlu: 5.408 ± 0.06	3.265LeuPhe: 3.265 ± 0.048	7.126LeuGly: 7.126 ± 0.06	1.966LeuHis: 1.966 ± 0.031	4.725LeuIle: 4.725 ± 0.052	3.062LeuLys: 3.062 ± 0.046	9.505LeuLeu: 9.505 ± 0.091	2.031LeuMet: 2.031 ± 0.033	2.639LeuAsn: 2.639 ± 0.04	5.569LeuPro: 5.569 ± 0.057	3.996LeuGln: 3.996 ± 0.046	7.027LeuArg: 7.027 ± 0.077	6.543LeuSer: 6.543 ± 0.062	5.318LeuThr: 5.318 ± 0.046	7.236LeuVal: 7.236 ± 0.073	1.271LeuTrp: 1.271 ± 0.026	2.021LeuTyr: 2.021 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.035MetAla: 2.035 ± 0.034	0.166MetCys: 0.166 ± 0.01	1.068MetAsp: 1.068 ± 0.023	1.065MetGlu: 1.065 ± 0.025	0.639MetPhe: 0.639 ± 0.017	1.351MetGly: 1.351 ± 0.026	0.48MetHis: 0.48 ± 0.013	1.198MetIle: 1.198 ± 0.024	0.83MetLys: 0.83 ± 0.021	2.23MetLeu: 2.23 ± 0.037	0.568MetMet: 0.568 ± 0.018	0.789MetAsn: 0.789 ± 0.02	1.299MetPro: 1.299 ± 0.025	0.949MetGln: 0.949 ± 0.023	1.557MetArg: 1.557 ± 0.026	1.422MetSer: 1.422 ± 0.03	1.435MetThr: 1.435 ± 0.026	1.469MetVal: 1.469 ± 0.03	0.217MetTrp: 0.217 ± 0.011	0.336MetTyr: 0.336 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.817AsnAla: 2.817 ± 0.039	0.341AsnCys: 0.341 ± 0.012	1.996AsnAsp: 1.996 ± 0.055	1.693AsnGlu: 1.693 ± 0.033	0.985AsnPhe: 0.985 ± 0.023	2.475AsnGly: 2.475 ± 0.052	0.753AsnHis: 0.753 ± 0.017	1.054AsnIle: 1.054 ± 0.02	0.628AsnLys: 0.628 ± 0.017	2.857AsnLeu: 2.857 ± 0.043	0.443AsnMet: 0.443 ± 0.015	0.882AsnAsn: 0.882 ± 0.032	1.965AsnPro: 1.965 ± 0.032	1.387AsnGln: 1.387 ± 0.027	2.439AsnArg: 2.439 ± 0.039	1.633AsnSer: 1.633 ± 0.036	1.381AsnThr: 1.381 ± 0.031	2.233AsnVal: 2.233 ± 0.039	0.463AsnTrp: 0.463 ± 0.014	0.74AsnTyr: 0.74 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	5.448ProAla: 5.448 ± 0.061	0.376ProCys: 0.376 ± 0.012	4.013ProAsp: 4.013 ± 0.047	4.145ProGlu: 4.145 ± 0.051	1.813ProPhe: 1.813 ± 0.032	4.425ProGly: 4.425 ± 0.055	1.111ProHis: 1.111 ± 0.023	2.751ProIle: 2.751 ± 0.042	1.667ProLys: 1.667 ± 0.028	4.674ProLeu: 4.674 ± 0.052	1.22ProMet: 1.22 ± 0.024	1.788ProAsn: 1.788 ± 0.034	3.393ProPro: 3.393 ± 0.053	2.068ProGln: 2.068 ± 0.031	3.113ProArg: 3.113 ± 0.036	3.942ProSer: 3.942 ± 0.051	3.342ProThr: 3.342 ± 0.045	3.978ProVal: 3.978 ± 0.045	0.803ProTrp: 0.803 ± 0.018	1.127ProTyr: 1.127 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.269GlnAla: 4.269 ± 0.046	0.41GlnCys: 0.41 ± 0.012	1.979GlnAsp: 1.979 ± 0.03	1.97GlnGlu: 1.97 ± 0.031	1.588GlnPhe: 1.588 ± 0.023	2.512GlnGly: 2.512 ± 0.04	0.977GlnHis: 0.977 ± 0.02	2.332GlnIle: 2.332 ± 0.032	1.185GlnLys: 1.185 ± 0.025	4.587GlnLeu: 4.587 ± 0.059	0.976GlnMet: 0.976 ± 0.023	1.192GlnAsn: 1.192 ± 0.026	2.732GlnPro: 2.732 ± 0.039	2.345GlnGln: 2.345 ± 0.051	3.583GlnArg: 3.583 ± 0.04	3.104GlnSer: 3.104 ± 0.04	2.685GlnThr: 2.685 ± 0.037	2.823GlnVal: 2.823 ± 0.039	0.799GlnTrp: 0.799 ± 0.022	0.928GlnTyr: 0.928 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	5.304ArgAla: 5.304 ± 0.056	0.902ArgCys: 0.902 ± 0.025	4.471ArgAsp: 4.471 ± 0.051	4.234ArgGlu: 4.234 ± 0.058	3.117ArgPhe: 3.117 ± 0.04	4.816ArgGly: 4.816 ± 0.052	1.661ArgHis: 1.661 ± 0.034	3.753ArgIle: 3.753 ± 0.049	2.146ArgLys: 2.146 ± 0.038	7.783ArgLeu: 7.783 ± 0.077	1.853ArgMet: 1.853 ± 0.035	2.044ArgAsn: 2.044 ± 0.03	3.839ArgPro: 3.839 ± 0.047	3.521ArgGln: 3.521 ± 0.046	6.379ArgArg: 6.379 ± 0.083	4.89ArgSer: 4.89 ± 0.054	3.465ArgThr: 3.465 ± 0.041	4.962ArgVal: 4.962 ± 0.05	1.572ArgTrp: 1.572 ± 0.031	2.029ArgTyr: 2.029 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.124SerAla: 6.124 ± 0.06	0.6SerCys: 0.6 ± 0.02	4.863SerAsp: 4.863 ± 0.058	4.104SerGlu: 4.104 ± 0.048	2.309SerPhe: 2.309 ± 0.036	6.35SerGly: 6.35 ± 0.075	1.318SerHis: 1.318 ± 0.025	3.261SerIle: 3.261 ± 0.04	1.855SerLys: 1.855 ± 0.036	6.537SerLeu: 6.537 ± 0.06	1.459SerMet: 1.459 ± 0.028	1.894SerAsn: 1.894 ± 0.034	3.875SerPro: 3.875 ± 0.044	2.646SerGln: 2.646 ± 0.043	4.363SerArg: 4.363 ± 0.046	4.223SerSer: 4.223 ± 0.061	3.378SerThr: 3.378 ± 0.042	4.964SerVal: 4.964 ± 0.058	0.885SerTrp: 0.885 ± 0.019	1.312SerTyr: 1.312 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	5.439ThrAla: 5.439 ± 0.058	0.519ThrCys: 0.519 ± 0.017	3.691ThrAsp: 3.691 ± 0.049	3.16ThrGlu: 3.16 ± 0.043	2.063ThrPhe: 2.063 ± 0.039	4.713ThrGly: 4.713 ± 0.067	1.058ThrHis: 1.058 ± 0.024	2.917ThrIle: 2.917 ± 0.046	1.392ThrLys: 1.392 ± 0.028	5.738ThrLeu: 5.738 ± 0.053	1.064ThrMet: 1.064 ± 0.022	1.475ThrAsn: 1.475 ± 0.032	3.454ThrPro: 3.454 ± 0.046	1.807ThrGln: 1.807 ± 0.032	3.388ThrArg: 3.388 ± 0.04	3.351ThrSer: 3.351 ± 0.047	3.085ThrThr: 3.085 ± 0.046	4.383ThrVal: 4.383 ± 0.073	0.759ThrTrp: 0.759 ± 0.019	1.227ThrTyr: 1.227 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	7.774ValAla: 7.774 ± 0.071	0.922ValCys: 0.922 ± 0.022	5.081ValAsp: 5.081 ± 0.056	4.114ValGlu: 4.114 ± 0.047	2.614ValPhe: 2.614 ± 0.038	5.454ValGly: 5.454 ± 0.058	1.489ValHis: 1.489 ± 0.026	3.733ValIle: 3.733 ± 0.05	1.89ValLys: 1.89 ± 0.033	6.795ValLeu: 6.795 ± 0.07	1.503ValMet: 1.503 ± 0.029	1.994ValAsn: 1.994 ± 0.042	3.821ValPro: 3.821 ± 0.05	2.805ValGln: 2.805 ± 0.035	5.152ValArg: 5.152 ± 0.055	4.793ValSer: 4.793 ± 0.057	4.319ValThr: 4.319 ± 0.066	5.743ValVal: 5.743 ± 0.064	1.033ValTrp: 1.033 ± 0.026	1.589ValTyr: 1.589 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.159TrpAla: 1.159 ± 0.022	0.214TrpCys: 0.214 ± 0.01	0.86TrpAsp: 0.86 ± 0.022	0.702TrpGlu: 0.702 ± 0.018	0.587TrpPhe: 0.587 ± 0.017	0.92TrpGly: 0.92 ± 0.023	0.44TrpHis: 0.44 ± 0.015	0.932TrpIle: 0.932 ± 0.021	0.616TrpLys: 0.616 ± 0.019	1.697TrpLeu: 1.697 ± 0.036	0.484TrpMet: 0.484 ± 0.015	0.57TrpAsn: 0.57 ± 0.014	0.717TrpPro: 0.717 ± 0.018	0.807TrpGln: 0.807 ± 0.019	1.083TrpArg: 1.083 ± 0.021	1.009TrpSer: 1.009 ± 0.023	0.891TrpThr: 0.891 ± 0.022	0.975TrpVal: 0.975 ± 0.023	0.297TrpTrp: 0.297 ± 0.012	0.373TrpTyr: 0.373 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.069TyrAla: 2.069 ± 0.03	0.305TyrCys: 0.305 ± 0.013	1.591TyrAsp: 1.591 ± 0.041	1.379TyrGlu: 1.379 ± 0.028	0.994TyrPhe: 0.994 ± 0.023	1.899TyrGly: 1.899 ± 0.032	0.602TyrHis: 0.602 ± 0.016	0.672TyrIle: 0.672 ± 0.016	0.504TyrLys: 0.504 ± 0.017	2.373TyrLeu: 2.373 ± 0.032	0.325TyrMet: 0.325 ± 0.013	0.624TyrAsn: 0.624 ± 0.019	1.189TyrPro: 1.189 ± 0.024	1.164TyrGln: 1.164 ± 0.023	2.202TyrArg: 2.202 ± 0.038	1.295TyrSer: 1.295 ± 0.023	1.03TyrThr: 1.03 ± 0.03	1.617TyrVal: 1.617 ± 0.03	0.401TyrTrp: 0.401 ± 0.013	0.676TyrTyr: 0.676 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5599 proteins (2301654 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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