Trichechus manatus latirostris (Florida manatee)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Afrotheria; Sirenia; Trichechidae; Trichechus;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31601 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Y9DNJ7|A0A2Y9DNJ7_TRIMA forkhead box protein F1 OS=Trichechus manatus latirostris OX=127582 GN=LOC101351765 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.75FQQLADD8 pKa = 5.0VADD11 pKa = 4.16KK12 pKa = 10.25WCSNTPFDD20 pKa = 6.16LIATEE25 pKa = 4.06EE26 pKa = 4.36TEE28 pKa = 4.33RR29 pKa = 11.84RR30 pKa = 11.84MDD32 pKa = 4.29FYY34 pKa = 11.47ADD36 pKa = 3.74PGVSFYY42 pKa = 10.98VLCPDD47 pKa = 4.64NGCGDD52 pKa = 3.96NFHH55 pKa = 6.3VWSEE59 pKa = 4.48SEE61 pKa = 3.97DD62 pKa = 3.63CLPFLQLAQDD72 pKa = 4.45YY73 pKa = 10.71ISSCGKK79 pKa = 8.66KK80 pKa = 8.01TLQEE84 pKa = 3.92VLEE87 pKa = 4.35KK88 pKa = 10.64VFKK91 pKa = 10.63SFRR94 pKa = 11.84PLLGLPDD101 pKa = 4.83ADD103 pKa = 3.93DD104 pKa = 4.59DD105 pKa = 4.62AFEE108 pKa = 4.77EE109 pKa = 4.52YY110 pKa = 10.51SADD113 pKa = 3.68VEE115 pKa = 4.38EE116 pKa = 5.09EE117 pKa = 4.2EE118 pKa = 5.29PEE120 pKa = 4.13ADD122 pKa = 3.66HH123 pKa = 6.65PQMGVSQQQ131 pKa = 3.0

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Y9R7J1|A0A2Y9R7J1_TRIMA Ena/VASP-like protein OS=Trichechus manatus latirostris OX=127582 GN=LOC101349543 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19079

12522

31601

19906729

31

34352

629.9

70.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.688 ± 0.014

2.166 ± 0.011

4.89 ± 0.009

7.319 ± 0.019

3.639 ± 0.011

6.187 ± 0.018

2.61 ± 0.006

4.506 ± 0.01

6.039 ± 0.017

9.899 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.006

3.778 ± 0.009

5.959 ± 0.023

4.883 ± 0.016

5.516 ± 0.011

8.507 ± 0.018

5.369 ± 0.013

5.996 ± 0.011

1.148 ± 0.005

2.661 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski