Amino acid dipepetide frequency for Trichechus manatus latirostris (Florida manatee)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.428AlaAla: 6.428 ± 0.04	1.343AlaCys: 1.343 ± 0.011	2.866AlaAsp: 2.866 ± 0.014	4.736AlaGlu: 4.736 ± 0.024	2.665AlaPhe: 2.665 ± 0.014	4.272AlaGly: 4.272 ± 0.024	1.498AlaHis: 1.498 ± 0.009	2.781AlaIle: 2.781 ± 0.016	3.414AlaLys: 3.414 ± 0.02	6.944AlaLeu: 6.944 ± 0.027	1.443AlaMet: 1.443 ± 0.01	2.065AlaAsn: 2.065 ± 0.012	3.801AlaPro: 3.801 ± 0.025	3.179AlaGln: 3.179 ± 0.017	3.431AlaArg: 3.431 ± 0.017	5.652AlaSer: 5.652 ± 0.023	3.489AlaThr: 3.489 ± 0.017	4.602AlaVal: 4.602 ± 0.018	0.735AlaTrp: 0.735 ± 0.006	1.528AlaTyr: 1.528 ± 0.011	0.008AlaXaa: 0.008 ± 0.001
Cys	1.201CysAla: 1.201 ± 0.012	0.597CysCys: 0.597 ± 0.009	0.992CysAsp: 0.992 ± 0.011	1.266CysGlu: 1.266 ± 0.012	0.839CysPhe: 0.839 ± 0.008	1.804CysGly: 1.804 ± 0.022	0.66CysHis: 0.66 ± 0.006	0.979CysIle: 0.979 ± 0.009	1.259CysLys: 1.259 ± 0.013	2.132CysLeu: 2.132 ± 0.016	0.42CysMet: 0.42 ± 0.005	0.841CysAsn: 0.841 ± 0.009	1.261CysPro: 1.261 ± 0.014	1.03CysGln: 1.03 ± 0.009	1.219CysArg: 1.219 ± 0.011	1.989CysSer: 1.989 ± 0.014	1.068CysThr: 1.068 ± 0.01	1.244CysVal: 1.244 ± 0.012	0.279CysTrp: 0.279 ± 0.004	0.576CysTyr: 0.576 ± 0.006	0.004CysXaa: 0.004 ± 0.0
Asp	2.794AspAla: 2.794 ± 0.014	1.06AspCys: 1.06 ± 0.01	2.732AspAsp: 2.732 ± 0.015	3.547AspGlu: 3.547 ± 0.017	2.143AspPhe: 2.143 ± 0.014	3.164AspGly: 3.164 ± 0.02	1.164AspHis: 1.164 ± 0.007	2.647AspIle: 2.647 ± 0.013	2.635AspLys: 2.635 ± 0.017	5.047AspLeu: 5.047 ± 0.019	1.125AspMet: 1.125 ± 0.007	1.753AspAsn: 1.753 ± 0.01	2.836AspPro: 2.836 ± 0.015	1.86AspGln: 1.86 ± 0.01	2.422AspArg: 2.422 ± 0.012	4.35AspSer: 4.35 ± 0.022	2.474AspThr: 2.474 ± 0.012	3.069AspVal: 3.069 ± 0.017	0.603AspTrp: 0.603 ± 0.006	1.468AspTyr: 1.468 ± 0.009	0.005AspXaa: 0.005 ± 0.001
Glu	5.066GluAla: 5.066 ± 0.024	1.639GluCys: 1.639 ± 0.027	4.534GluAsp: 4.534 ± 0.018	8.23GluGlu: 8.23 ± 0.045	2.066GluPhe: 2.066 ± 0.012	4.116GluGly: 4.116 ± 0.02	1.532GluHis: 1.532 ± 0.009	3.28GluIle: 3.28 ± 0.018	5.791GluLys: 5.791 ± 0.034	6.608GluLeu: 6.608 ± 0.031	1.729GluMet: 1.729 ± 0.012	3.32GluAsn: 3.32 ± 0.016	3.142GluPro: 3.142 ± 0.023	3.328GluGln: 3.328 ± 0.022	4.08GluArg: 4.08 ± 0.022	4.696GluSer: 4.696 ± 0.023	3.503GluThr: 3.503 ± 0.019	4.239GluVal: 4.239 ± 0.021	0.678GluTrp: 0.678 ± 0.006	1.61GluTyr: 1.61 ± 0.011	0.006GluXaa: 0.006 ± 0.001
Phe	1.956PheAla: 1.956 ± 0.011	0.944PheCys: 0.944 ± 0.01	1.665PheAsp: 1.665 ± 0.011	2.038PheGlu: 2.038 ± 0.011	1.616PhePhe: 1.616 ± 0.013	2.144PheGly: 2.144 ± 0.014	1.055PheHis: 1.055 ± 0.009	1.898PheIle: 1.898 ± 0.014	1.81PheLys: 1.81 ± 0.012	4.071PheLeu: 4.071 ± 0.019	0.799PheMet: 0.799 ± 0.007	1.343PheAsn: 1.343 ± 0.01	1.95PhePro: 1.95 ± 0.011	1.807PheGln: 1.807 ± 0.011	1.931PheArg: 1.931 ± 0.013	3.551PheSer: 3.551 ± 0.018	2.039PheThr: 2.039 ± 0.013	2.119PheVal: 2.119 ± 0.012	0.466PheTrp: 0.466 ± 0.005	1.182PheTyr: 1.182 ± 0.01	0.006PheXaa: 0.006 ± 0.001
Gly	4.185GlyAla: 4.185 ± 0.022	1.215GlyCys: 1.215 ± 0.01	3.007GlyAsp: 3.007 ± 0.016	4.133GlyGlu: 4.133 ± 0.024	2.306GlyPhe: 2.306 ± 0.016	4.632GlyGly: 4.632 ± 0.035	1.638GlyHis: 1.638 ± 0.013	2.698GlyIle: 2.698 ± 0.014	3.874GlyLys: 3.874 ± 0.023	5.611GlyLeu: 5.611 ± 0.023	1.312GlyMet: 1.312 ± 0.01	2.365GlyAsn: 2.365 ± 0.013	3.833GlyPro: 3.833 ± 0.033	2.702GlyGln: 2.702 ± 0.015	3.569GlyArg: 3.569 ± 0.017	5.555GlySer: 5.555 ± 0.025	3.463GlyThr: 3.463 ± 0.017	3.352GlyVal: 3.352 ± 0.017	0.753GlyTrp: 0.753 ± 0.008	1.658GlyTyr: 1.658 ± 0.014	0.01GlyXaa: 0.01 ± 0.001
His	1.285HisAla: 1.285 ± 0.009	0.716HisCys: 0.716 ± 0.007	0.89HisAsp: 0.89 ± 0.006	1.343HisGlu: 1.343 ± 0.009	1.08HisPhe: 1.08 ± 0.008	1.446HisGly: 1.446 ± 0.011	0.922HisHis: 0.922 ± 0.011	1.309HisIle: 1.309 ± 0.01	1.348HisLys: 1.348 ± 0.01	2.983HisLeu: 2.983 ± 0.015	0.618HisMet: 0.618 ± 0.006	0.886HisAsn: 0.886 ± 0.008	1.607HisPro: 1.607 ± 0.011	1.445HisGln: 1.445 ± 0.015	1.557HisArg: 1.557 ± 0.01	2.47HisSer: 2.47 ± 0.015	1.568HisThr: 1.568 ± 0.016	1.497HisVal: 1.497 ± 0.01	0.329HisTrp: 0.329 ± 0.005	0.797HisTyr: 0.797 ± 0.006	0.003HisXaa: 0.003 ± 0.0
Ile	2.603IleAla: 2.603 ± 0.014	1.083IleCys: 1.083 ± 0.009	2.067IleAsp: 2.067 ± 0.012	2.719IleGlu: 2.719 ± 0.016	1.931IlePhe: 1.931 ± 0.014	2.188IleGly: 2.188 ± 0.013	1.43IleHis: 1.43 ± 0.013	2.451IleIle: 2.451 ± 0.016	2.75IleLys: 2.75 ± 0.015	4.646IleLeu: 4.646 ± 0.023	1.026IleMet: 1.026 ± 0.007	1.903IleAsn: 1.903 ± 0.012	2.656IlePro: 2.656 ± 0.013	2.401IleGln: 2.401 ± 0.014	2.352IleArg: 2.352 ± 0.013	3.825IleSer: 3.825 ± 0.018	2.629IleThr: 2.629 ± 0.015	2.515IleVal: 2.515 ± 0.014	0.501IleTrp: 0.501 ± 0.005	1.375IleTyr: 1.375 ± 0.011	0.006IleXaa: 0.006 ± 0.001
Lys	4.035LysAla: 4.035 ± 0.022	1.179LysCys: 1.179 ± 0.012	3.338LysAsp: 3.338 ± 0.017	5.508LysGlu: 5.508 ± 0.031	1.752LysPhe: 1.752 ± 0.011	3.209LysGly: 3.209 ± 0.02	1.479LysHis: 1.479 ± 0.011	2.868LysIle: 2.868 ± 0.016	4.97LysLys: 4.97 ± 0.029	5.417LysLeu: 5.417 ± 0.025	1.508LysMet: 1.508 ± 0.011	2.545LysAsn: 2.545 ± 0.013	3.208LysPro: 3.208 ± 0.022	2.803LysGln: 2.803 ± 0.018	3.368LysArg: 3.368 ± 0.018	4.153LysSer: 4.153 ± 0.024	3.29LysThr: 3.29 ± 0.017	3.541LysVal: 3.541 ± 0.02	0.613LysTrp: 0.613 ± 0.007	1.602LysTyr: 1.602 ± 0.011	0.007LysXaa: 0.007 ± 0.001
Leu	6.556LeuAla: 6.556 ± 0.027	2.154LeuCys: 2.154 ± 0.014	4.749LeuAsp: 4.749 ± 0.022	7.383LeuGlu: 7.383 ± 0.038	3.391LeuPhe: 3.391 ± 0.02	5.674LeuGly: 5.674 ± 0.025	2.768LeuHis: 2.768 ± 0.015	4.015LeuIle: 4.015 ± 0.02	6.067LeuLys: 6.067 ± 0.031	10.67LeuLeu: 10.67 ± 0.051	2.087LeuMet: 2.087 ± 0.012	3.649LeuAsn: 3.649 ± 0.016	5.84LeuPro: 5.84 ± 0.025	5.926LeuGln: 5.926 ± 0.034	5.75LeuArg: 5.75 ± 0.022	8.083LeuSer: 8.083 ± 0.027	5.186LeuThr: 5.186 ± 0.02	5.44LeuVal: 5.44 ± 0.023	1.082LeuTrp: 1.082 ± 0.009	2.501LeuTyr: 2.501 ± 0.013	0.013LeuXaa: 0.013 ± 0.001
Met	1.893MetAla: 1.893 ± 0.01	0.396MetCys: 0.396 ± 0.005	1.281MetAsp: 1.281 ± 0.01	1.936MetGlu: 1.936 ± 0.012	0.748MetPhe: 0.748 ± 0.007	1.245MetGly: 1.245 ± 0.009	0.49MetHis: 0.49 ± 0.006	0.875MetIle: 0.875 ± 0.008	1.506MetLys: 1.506 ± 0.01	2.018MetLeu: 2.018 ± 0.012	0.582MetMet: 0.582 ± 0.007	0.937MetAsn: 0.937 ± 0.007	1.085MetPro: 1.085 ± 0.012	0.954MetGln: 0.954 ± 0.008	1.055MetArg: 1.055 ± 0.007	1.6MetSer: 1.6 ± 0.01	1.149MetThr: 1.149 ± 0.008	1.392MetVal: 1.392 ± 0.009	0.235MetTrp: 0.235 ± 0.003	0.604MetTyr: 0.604 ± 0.006	0.002MetXaa: 0.002 ± 0.0
Asn	2.064AsnAla: 2.064 ± 0.008	0.845AsnCys: 0.845 ± 0.007	1.578AsnAsp: 1.578 ± 0.01	2.363AsnGlu: 2.363 ± 0.013	1.503AsnPhe: 1.503 ± 0.01	2.459AsnGly: 2.459 ± 0.016	1.012AsnHis: 1.012 ± 0.008	2.197AsnIle: 2.197 ± 0.013	2.334AsnLys: 2.334 ± 0.014	3.898AsnLeu: 3.898 ± 0.019	0.931AsnMet: 0.931 ± 0.007	1.591AsnAsn: 1.591 ± 0.013	2.183AsnPro: 2.183 ± 0.012	1.762AsnGln: 1.762 ± 0.012	1.883AsnArg: 1.883 ± 0.009	3.321AsnSer: 3.321 ± 0.017	2.057AsnThr: 2.057 ± 0.013	2.228AsnVal: 2.228 ± 0.013	0.448AsnTrp: 0.448 ± 0.005	1.124AsnTyr: 1.124 ± 0.01	0.005AsnXaa: 0.005 ± 0.001
Pro	4.489ProAla: 4.489 ± 0.029	1.051ProCys: 1.051 ± 0.011	2.71ProAsp: 2.71 ± 0.015	4.309ProGlu: 4.309 ± 0.022	1.888ProPhe: 1.888 ± 0.013	4.759ProGly: 4.759 ± 0.045	1.409ProHis: 1.409 ± 0.01	1.878ProIle: 1.878 ± 0.013	2.811ProLys: 2.811 ± 0.022	5.166ProLeu: 5.166 ± 0.023	1.067ProMet: 1.067 ± 0.008	1.837ProAsn: 1.837 ± 0.011	5.643ProPro: 5.643 ± 0.047	2.767ProGln: 2.767 ± 0.018	3.119ProArg: 3.119 ± 0.02	5.582ProSer: 5.582 ± 0.03	3.052ProThr: 3.052 ± 0.019	3.791ProVal: 3.791 ± 0.02	0.635ProTrp: 0.635 ± 0.006	1.621ProTyr: 1.621 ± 0.019	0.011ProXaa: 0.011 ± 0.001
Gln	3.476GlnAla: 3.476 ± 0.02	0.941GlnCys: 0.941 ± 0.009	2.381GlnAsp: 2.381 ± 0.013	4.032GlnGlu: 4.032 ± 0.023	1.391GlnPhe: 1.391 ± 0.009	2.746GlnGly: 2.746 ± 0.017	1.325GlnHis: 1.325 ± 0.011	2.095GlnIle: 2.095 ± 0.012	3.109GlnLys: 3.109 ± 0.019	4.924GlnLeu: 4.924 ± 0.028	1.166GlnMet: 1.166 ± 0.008	1.932GlnAsn: 1.932 ± 0.013	2.737GlnPro: 2.737 ± 0.019	3.358GlnGln: 3.358 ± 0.037	3.031GlnArg: 3.031 ± 0.018	3.262GlnSer: 3.262 ± 0.017	2.377GlnThr: 2.377 ± 0.012	2.837GlnVal: 2.837 ± 0.015	0.532GlnTrp: 0.532 ± 0.005	1.175GlnTyr: 1.175 ± 0.008	0.005GlnXaa: 0.005 ± 0.0
Arg	3.594ArgAla: 3.594 ± 0.018	1.126ArgCys: 1.126 ± 0.011	2.714ArgAsp: 2.714 ± 0.013	4.002ArgGlu: 4.002 ± 0.02	1.8ArgPhe: 1.8 ± 0.01	3.383ArgGly: 3.383 ± 0.019	1.514ArgHis: 1.514 ± 0.01	2.483ArgIle: 2.483 ± 0.017	3.759ArgLys: 3.759 ± 0.017	5.259ArgLeu: 5.259 ± 0.027	1.176ArgMet: 1.176 ± 0.008	2.163ArgAsn: 2.163 ± 0.013	3.117ArgPro: 3.117 ± 0.02	2.612ArgGln: 2.612 ± 0.016	4.209ArgArg: 4.209 ± 0.022	4.293ArgSer: 4.293 ± 0.024	2.843ArgThr: 2.843 ± 0.014	3.026ArgVal: 3.026 ± 0.016	0.65ArgTrp: 0.65 ± 0.007	1.433ArgTyr: 1.433 ± 0.009	0.008ArgXaa: 0.008 ± 0.001
Ser	5.315SerAla: 5.315 ± 0.02	1.787SerCys: 1.787 ± 0.012	4.048SerAsp: 4.048 ± 0.022	5.484SerGlu: 5.484 ± 0.025	3.075SerPhe: 3.075 ± 0.015	5.55SerGly: 5.55 ± 0.022	2.222SerHis: 2.222 ± 0.015	3.307SerIle: 3.307 ± 0.015	4.359SerLys: 4.359 ± 0.022	8.322SerLeu: 8.322 ± 0.031	1.614SerMet: 1.614 ± 0.01	2.872SerAsn: 2.872 ± 0.014	5.896SerPro: 5.896 ± 0.035	4.047SerGln: 4.047 ± 0.022	4.537SerArg: 4.537 ± 0.022	9.835SerSer: 9.835 ± 0.048	4.628SerThr: 4.628 ± 0.021	5.01SerVal: 5.01 ± 0.017	1.054SerTrp: 1.054 ± 0.01	2.094SerTyr: 2.094 ± 0.015	0.012SerXaa: 0.012 ± 0.001
Thr	3.702ThrAla: 3.702 ± 0.017	1.243ThrCys: 1.243 ± 0.012	2.5ThrAsp: 2.5 ± 0.013	3.703ThrGlu: 3.703 ± 0.018	2.129ThrPhe: 2.129 ± 0.014	3.531ThrGly: 3.531 ± 0.018	1.387ThrHis: 1.387 ± 0.014	2.404ThrIle: 2.404 ± 0.015	2.778ThrLys: 2.778 ± 0.015	5.338ThrLeu: 5.338 ± 0.021	1.138ThrMet: 1.138 ± 0.008	1.787ThrAsn: 1.787 ± 0.011	3.492ThrPro: 3.492 ± 0.025	2.308ThrGln: 2.308 ± 0.012	2.412ThrArg: 2.412 ± 0.011	4.801ThrSer: 4.801 ± 0.025	3.075ThrThr: 3.075 ± 0.025	3.893ThrVal: 3.893 ± 0.02	0.664ThrTrp: 0.664 ± 0.006	1.397ThrTyr: 1.397 ± 0.009	0.007ThrXaa: 0.007 ± 0.001
Val	4.11ValAla: 4.11 ± 0.018	1.414ValCys: 1.414 ± 0.01	2.949ValAsp: 2.949 ± 0.017	3.896ValGlu: 3.896 ± 0.018	2.401ValPhe: 2.401 ± 0.013	3.241ValGly: 3.241 ± 0.015	1.553ValHis: 1.553 ± 0.01	2.959ValIle: 2.959 ± 0.017	3.514ValLys: 3.514 ± 0.018	6.125ValLeu: 6.125 ± 0.022	1.317ValMet: 1.317 ± 0.009	2.329ValAsn: 2.329 ± 0.012	3.547ValPro: 3.547 ± 0.019	2.743ValGln: 2.743 ± 0.014	2.977ValArg: 2.977 ± 0.014	4.95ValSer: 4.95 ± 0.019	3.701ValThr: 3.701 ± 0.023	3.944ValVal: 3.944 ± 0.02	0.68ValTrp: 0.68 ± 0.007	1.604ValTyr: 1.604 ± 0.011	0.006ValXaa: 0.006 ± 0.001
Trp	0.743TrpAla: 0.743 ± 0.007	0.242TrpCys: 0.242 ± 0.004	0.627TrpAsp: 0.627 ± 0.006	0.774TrpGlu: 0.774 ± 0.006	0.441TrpPhe: 0.441 ± 0.005	0.688TrpGly: 0.688 ± 0.008	0.295TrpHis: 0.295 ± 0.004	0.542TrpIle: 0.542 ± 0.006	0.793TrpLys: 0.793 ± 0.007	1.155TrpLeu: 1.155 ± 0.009	0.313TrpMet: 0.313 ± 0.004	0.541TrpAsn: 0.541 ± 0.006	0.489TrpPro: 0.489 ± 0.005	0.521TrpGln: 0.521 ± 0.006	0.695TrpArg: 0.695 ± 0.007	0.843TrpSer: 0.843 ± 0.008	0.62TrpThr: 0.62 ± 0.007	0.642TrpVal: 0.642 ± 0.006	0.189TrpTrp: 0.189 ± 0.003	0.322TrpTyr: 0.322 ± 0.005	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.374TyrAla: 1.374 ± 0.01	0.682TyrCys: 0.682 ± 0.006	1.266TyrAsp: 1.266 ± 0.011	1.78TyrGlu: 1.78 ± 0.013	1.218TyrPhe: 1.218 ± 0.009	1.608TyrGly: 1.608 ± 0.011	0.744TyrHis: 0.744 ± 0.007	1.388TyrIle: 1.388 ± 0.011	1.505TyrLys: 1.505 ± 0.012	2.639TyrLeu: 2.639 ± 0.014	0.61TyrMet: 0.61 ± 0.007	1.122TyrAsn: 1.122 ± 0.009	1.262TyrPro: 1.262 ± 0.008	1.271TyrGln: 1.271 ± 0.011	1.56TyrArg: 1.56 ± 0.011	2.246TyrSer: 2.246 ± 0.013	1.475TyrThr: 1.475 ± 0.011	1.57TyrVal: 1.57 ± 0.01	0.353TyrTrp: 0.353 ± 0.005	0.932TyrTyr: 0.932 ± 0.008	0.003TyrXaa: 0.003 ± 0.0
Xaa	0.009XaaAla: 0.009 ± 0.001	0.003XaaCys: 0.003 ± 0.0	0.005XaaAsp: 0.005 ± 0.001	0.008XaaGlu: 0.008 ± 0.001	0.006XaaPhe: 0.006 ± 0.001	0.011XaaGly: 0.011 ± 0.001	0.003XaaHis: 0.003 ± 0.0	0.004XaaIle: 0.004 ± 0.001	0.007XaaLys: 0.007 ± 0.001	0.012XaaLeu: 0.012 ± 0.001	0.003XaaMet: 0.003 ± 0.0	0.004XaaAsn: 0.004 ± 0.0	0.009XaaPro: 0.009 ± 0.001	0.006XaaGln: 0.006 ± 0.001	0.007XaaArg: 0.007 ± 0.001	0.01XaaSer: 0.01 ± 0.001	0.005XaaThr: 0.005 ± 0.001	0.01XaaVal: 0.01 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.003XaaTyr: 0.003 ± 0.0	0.289XaaXaa: 0.289 ± 0.023

Statistics based on 31601 proteins (19906729 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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