Hydromonas duriensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Hydromonas

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6Y9M4|A0A4R6Y9M4_9BURK Cytochrome c biogenesis protein OS=Hydromonas duriensis OX=1527608 GN=DFR44_10566 PE=4 SV=1
MM1 pKa = 7.02TAVLTVFIVFLLFCVLSSAFSSSQKK26 pKa = 10.27KK27 pKa = 10.29GSQTGSQTSNSSIDD41 pKa = 3.46TSASTDD47 pKa = 3.67SNDD50 pKa = 3.18ACSDD54 pKa = 3.53GGDD57 pKa = 4.0CGDD60 pKa = 4.62GGGGCDD66 pKa = 3.43

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6YAI6|A0A4R6YAI6_9BURK UIT6 family transporter OS=Hydromonas duriensis OX=1527608 GN=DFR44_103103 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.82GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.02TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.51GRR39 pKa = 11.84TRR41 pKa = 11.84LAVV44 pKa = 3.19

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2252

0

2252

707475

29

3118

314.2

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.062 ± 0.068

0.974 ± 0.017

5.347 ± 0.039

5.39 ± 0.053

3.954 ± 0.04

7.0 ± 0.065

2.535 ± 0.032

5.712 ± 0.038

5.059 ± 0.044

10.047 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.74 ± 0.027

4.243 ± 0.052

4.084 ± 0.032

4.485 ± 0.038

4.927 ± 0.052

6.159 ± 0.061

5.719 ± 0.06

7.37 ± 0.052

1.329 ± 0.024

2.863 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski