Lentinula edodes negative-strand RNA virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Lentinuvirus; Lentinula lentinuvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P2VRN6|A0A4P2VRN6_9VIRU Replicase OS=Lentinula edodes negative-strand RNA virus 2 OX=2547431 GN=L PE=4 SV=1
MM1 pKa = 7.53NSNNKK6 pKa = 9.02LLKK9 pKa = 10.2IFGGSRR15 pKa = 11.84RR16 pKa = 11.84GKK18 pKa = 9.81VEE20 pKa = 3.7NDD22 pKa = 3.12EE23 pKa = 4.57EE24 pKa = 4.04EE25 pKa = 4.49GKK27 pKa = 10.66AVEE30 pKa = 4.48RR31 pKa = 11.84EE32 pKa = 4.03EE33 pKa = 5.3APPQAAPMKK42 pKa = 10.28HH43 pKa = 5.74FKK45 pKa = 10.51FPNKK49 pKa = 7.53YY50 pKa = 10.61ARR52 pKa = 11.84MEE54 pKa = 3.96EE55 pKa = 4.34HH56 pKa = 6.53IMNAEE61 pKa = 3.95RR62 pKa = 11.84SSGPFEE68 pKa = 4.05YY69 pKa = 10.75VAIASRR75 pKa = 11.84EE76 pKa = 3.7TSTFKK81 pKa = 11.06KK82 pKa = 10.38LLEE85 pKa = 4.32ALSVTKK91 pKa = 10.73ASVRR95 pKa = 11.84SYY97 pKa = 11.48DD98 pKa = 4.37LSFDD102 pKa = 4.14DD103 pKa = 5.33LPIYY107 pKa = 10.44NLSKK111 pKa = 10.2RR112 pKa = 11.84SSSISISSPLSLALQKK128 pKa = 10.61KK129 pKa = 9.48DD130 pKa = 5.29LHH132 pKa = 6.0FCWLKK137 pKa = 9.56EE138 pKa = 3.84VYY140 pKa = 10.12IFFVPTQSFSSSYY153 pKa = 11.25SEE155 pKa = 3.77ITFEE159 pKa = 4.95LNDD162 pKa = 3.28NRR164 pKa = 11.84FVEE167 pKa = 4.62EE168 pKa = 4.1SLVRR172 pKa = 11.84SITFPSNVGINGHH185 pKa = 6.35FSLDD189 pKa = 3.41YY190 pKa = 11.29SVFKK194 pKa = 10.73DD195 pKa = 4.14DD196 pKa = 5.32LPMISFSVKK205 pKa = 10.2CKK207 pKa = 10.5NSYY210 pKa = 10.2LKK212 pKa = 10.79EE213 pKa = 3.98GVVWGSLKK221 pKa = 10.84LVIQTKK227 pKa = 10.27FSDD230 pKa = 3.26QALIVSTTRR239 pKa = 11.84TAAILQMADD248 pKa = 3.24TTMKK252 pKa = 10.43TYY254 pKa = 10.99DD255 pKa = 4.16RR256 pKa = 11.84DD257 pKa = 4.09PNHH260 pKa = 7.37LDD262 pKa = 4.85LMFDD266 pKa = 4.09DD267 pKa = 6.02PDD269 pKa = 4.85LEE271 pKa = 4.28MMKK274 pKa = 10.46KK275 pKa = 9.77FAKK278 pKa = 9.44QGRR281 pKa = 11.84IVDD284 pKa = 3.84HH285 pKa = 6.26NQALGNRR292 pKa = 11.84EE293 pKa = 4.29RR294 pKa = 11.84IAMSGSEE301 pKa = 4.06AGSIEE306 pKa = 4.12GSQSGNSRR314 pKa = 11.84VKK316 pKa = 10.66EE317 pKa = 3.57EE318 pKa = 3.96WLRR321 pKa = 11.84GTRR324 pKa = 11.84FSRR327 pKa = 11.84GRR329 pKa = 11.84VSPVASVEE337 pKa = 3.85EE338 pKa = 4.45DD339 pKa = 3.4VRR341 pKa = 11.84SDD343 pKa = 3.54DD344 pKa = 3.99QEE346 pKa = 4.16VGEE349 pKa = 5.71FDD351 pKa = 3.64LTPNDD356 pKa = 3.8FTSSGMTEE364 pKa = 3.78RR365 pKa = 11.84AFSKK369 pKa = 10.71SAMKK373 pKa = 10.47APRR376 pKa = 11.84RR377 pKa = 11.84SLKK380 pKa = 10.77DD381 pKa = 3.18PSTSPEE387 pKa = 3.67TAANTSRR394 pKa = 11.84NEE396 pKa = 4.12GPSNWRR402 pKa = 11.84EE403 pKa = 3.91DD404 pKa = 3.67EE405 pKa = 4.19EE406 pKa = 4.4EE407 pKa = 3.96QRR409 pKa = 11.84TTARR413 pKa = 11.84NKK415 pKa = 10.01SVYY418 pKa = 9.78IYY420 pKa = 9.5EE421 pKa = 4.52GPP423 pKa = 3.37

Molecular weight: 47.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P2W265|A0A4P2W265_9VIRU Nucleocapsid protein OS=Lentinula edodes negative-strand RNA virus 2 OX=2547431 GN=ORF2b PE=4 SV=1
MM1 pKa = 7.5AVALRR6 pKa = 11.84NRR8 pKa = 11.84FNGLQRR14 pKa = 11.84TDD16 pKa = 2.71VSLAVGTILTQTEE29 pKa = 3.8ISAVLEE35 pKa = 3.74ADD37 pKa = 3.02INIIQEE43 pKa = 4.4RR44 pKa = 11.84IAGEE48 pKa = 3.89FIIDD52 pKa = 3.43VDD54 pKa = 4.66SINAALEE61 pKa = 3.95LLSYY65 pKa = 10.79EE66 pKa = 4.27GFDD69 pKa = 3.45PRR71 pKa = 11.84IVYY74 pKa = 10.15AHH76 pKa = 7.49LAHH79 pKa = 6.45IQKK82 pKa = 10.12AGNIGWPQLKK92 pKa = 9.52EE93 pKa = 4.02DD94 pKa = 4.18LMIMLVIHH102 pKa = 6.29HH103 pKa = 6.36VRR105 pKa = 11.84GNINSNNMKK114 pKa = 10.37SMKK117 pKa = 10.25SEE119 pKa = 3.77AKK121 pKa = 10.54NLINEE126 pKa = 4.75LFAKK130 pKa = 8.94YY131 pKa = 9.97HH132 pKa = 5.65IVVAKK137 pKa = 8.73DD138 pKa = 3.06TSKK141 pKa = 11.12RR142 pKa = 11.84LAVTLPRR149 pKa = 11.84LGASFPYY156 pKa = 10.08QVAQIAKK163 pKa = 8.66ICNRR167 pKa = 11.84DD168 pKa = 3.11FAGFCEE174 pKa = 4.62SAVLPAAMKK183 pKa = 9.13STSFPSLVPKK193 pKa = 9.02STKK196 pKa = 9.63ISLLMTVAYY205 pKa = 9.79NAYY208 pKa = 9.08ATDD211 pKa = 3.49QACALKK217 pKa = 10.39KK218 pKa = 10.59LNYY221 pKa = 10.31LEE223 pKa = 4.38MKK225 pKa = 10.61EE226 pKa = 4.0EE227 pKa = 3.84DD228 pKa = 3.39RR229 pKa = 11.84KK230 pKa = 10.82RR231 pKa = 11.84LFLEE235 pKa = 3.68QQRR238 pKa = 11.84YY239 pKa = 6.76TDD241 pKa = 3.06ISYY244 pKa = 10.94NSPVLDD250 pKa = 3.72KK251 pKa = 11.11DD252 pKa = 3.42HH253 pKa = 6.87RR254 pKa = 11.84QAALFNLGLGNSTVYY269 pKa = 10.48KK270 pKa = 10.76ALLSCTKK277 pKa = 9.83YY278 pKa = 10.99SSVDD282 pKa = 3.07TSLFCSEE289 pKa = 4.2TEE291 pKa = 3.94YY292 pKa = 11.1LGEE295 pKa = 4.51IISYY299 pKa = 9.23QASADD304 pKa = 4.34LIAKK308 pKa = 7.94MVASRR313 pKa = 11.84VSGPSS318 pKa = 2.87

Molecular weight: 35.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3050

318

2309

1016.7

116.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.656 ± 1.937

1.377 ± 0.285

6.197 ± 0.459

8.23 ± 0.746

5.344 ± 0.511

4.492 ± 0.152

2.328 ± 0.373

6.361 ± 0.644

7.738 ± 0.59

8.918 ± 0.695

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.115 ± 0.086

4.852 ± 0.156

3.639 ± 0.281

2.492 ± 0.373

5.508 ± 0.372

9.508 ± 1.028

4.918 ± 0.088

5.902 ± 0.103

1.049 ± 0.221

3.377 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski