Anaeroglobus geminatus F0357
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2148 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9YJ58|G9YJ58_9FIRM Histidinol dehydrogenase OS=Anaeroglobus geminatus F0357 OX=861450 GN=hisD PE=3 SV=1
MM1 pKa = 7.54 ANVSLGMTGTRR12 pKa = 11.84 VASVQNMLIGTGYY25 pKa = 10.37 LADD28 pKa = 4.17 GADD31 pKa = 3.62 GVFGSGTQAAVQRR44 pKa = 11.84 FQADD48 pKa = 2.62 HH49 pKa = 6.77 GLTVDD54 pKa = 5.15 GIVGTQTMNALSAASGQPIPVDD76 pKa = 3.12 NTIVMEE82 pKa = 4.36 ATAYY86 pKa = 8.9 TADD89 pKa = 3.79 DD90 pKa = 4.88 PGNSGYY96 pKa = 8.42 TATGQPLAYY105 pKa = 9.54 GVASVDD111 pKa = 3.42 PDD113 pKa = 4.13 VIPLGSRR120 pKa = 11.84 LYY122 pKa = 10.42 IEE124 pKa = 5.2 GYY126 pKa = 9.99 GYY128 pKa = 11.17 AVAADD133 pKa = 3.49 TGGAIVGNRR142 pKa = 11.84 IDD144 pKa = 4.02 LAMDD148 pKa = 4.05 SVADD152 pKa = 3.7 ALNFGRR158 pKa = 11.84 RR159 pKa = 11.84 DD160 pKa = 3.65 VVVHH164 pKa = 5.74 VLL166 pKa = 3.34
Molecular weight: 16.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.948
IPC_protein 3.935
Toseland 3.706
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.961
Rodwell 3.77
Grimsley 3.617
Solomon 3.948
Lehninger 3.91
Nozaki 4.088
DTASelect 4.406
Thurlkill 3.783
EMBOSS 3.961
Sillero 4.075
Patrickios 1.964
IPC_peptide 3.935
IPC2_peptide 4.037
IPC2.peptide.svr19 3.945
Protein with the highest isoelectric point:
>tr|G9YFC4|G9YFC4_9FIRM Ferredoxin OS=Anaeroglobus geminatus F0357 OX=861450 GN=HMPREF0080_00335 PE=4 SV=1
MM1 pKa = 7.57 HH2 pKa = 7.28 GNDD5 pKa = 3.85 EE6 pKa = 4.81 KK7 pKa = 11.03 ILNIPEE13 pKa = 4.16 KK14 pKa = 10.15 PAFIYY19 pKa = 10.84 LPVFCSLCRR28 pKa = 11.84 FNMHH32 pKa = 6.09 NTPSVLPRR40 pKa = 11.84 RR41 pKa = 11.84 IGAVPAVRR49 pKa = 11.84 TAAGIKK55 pKa = 9.2 IVRR58 pKa = 11.84 VFDD61 pKa = 3.68 TYY63 pKa = 10.98 RR64 pKa = 11.84 GDD66 pKa = 4.45 DD67 pKa = 3.57 RR68 pKa = 11.84 FHH70 pKa = 6.14 QLLRR74 pKa = 11.84 NRR76 pKa = 11.84 ADD78 pKa = 2.74 ISGRR82 pKa = 11.84 IRR84 pKa = 11.84 KK85 pKa = 8.53 II86 pKa = 3.17
Molecular weight: 9.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.619
IPC_protein 10.526
Toseland 10.716
ProMoST 10.467
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.921
Nozaki 10.716
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.75
IPC2.peptide.svr19 8.577
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2148
0
2148
542794
23
1655
252.7
27.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.386 ± 0.072
1.333 ± 0.023
5.505 ± 0.047
6.486 ± 0.06
4.006 ± 0.039
7.872 ± 0.056
2.047 ± 0.025
6.741 ± 0.052
5.88 ± 0.05
9.012 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.833 ± 0.026
3.7 ± 0.042
3.796 ± 0.037
2.755 ± 0.029
5.617 ± 0.053
5.199 ± 0.036
5.781 ± 0.047
7.564 ± 0.054
0.87 ± 0.019
3.617 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here