Anaeroglobus geminatus F0357

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Anaeroglobus; Anaeroglobus geminatus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|G9YJ58|G9YJ58_9FIRM Histidinol dehydrogenase OS=Anaeroglobus geminatus F0357 OX=861450 GN=hisD PE=3 SV=1
MM1 pKa = 7.54ANVSLGMTGTRR12 pKa = 11.84VASVQNMLIGTGYY25 pKa = 10.37LADD28 pKa = 4.17GADD31 pKa = 3.62GVFGSGTQAAVQRR44 pKa = 11.84FQADD48 pKa = 2.62HH49 pKa = 6.77GLTVDD54 pKa = 5.15GIVGTQTMNALSAASGQPIPVDD76 pKa = 3.12NTIVMEE82 pKa = 4.36ATAYY86 pKa = 8.9TADD89 pKa = 3.79DD90 pKa = 4.88PGNSGYY96 pKa = 8.42TATGQPLAYY105 pKa = 9.54GVASVDD111 pKa = 3.42PDD113 pKa = 4.13VIPLGSRR120 pKa = 11.84LYY122 pKa = 10.42IEE124 pKa = 5.2GYY126 pKa = 9.99GYY128 pKa = 11.17AVAADD133 pKa = 3.49TGGAIVGNRR142 pKa = 11.84IDD144 pKa = 4.02LAMDD148 pKa = 4.05SVADD152 pKa = 3.7ALNFGRR158 pKa = 11.84RR159 pKa = 11.84DD160 pKa = 3.65VVVHH164 pKa = 5.74VLL166 pKa = 3.34

Molecular weight:
16.83 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|G9YFC4|G9YFC4_9FIRM Ferredoxin OS=Anaeroglobus geminatus F0357 OX=861450 GN=HMPREF0080_00335 PE=4 SV=1
MM1 pKa = 7.57HH2 pKa = 7.28GNDD5 pKa = 3.85EE6 pKa = 4.81KK7 pKa = 11.03ILNIPEE13 pKa = 4.16KK14 pKa = 10.15PAFIYY19 pKa = 10.84LPVFCSLCRR28 pKa = 11.84FNMHH32 pKa = 6.09NTPSVLPRR40 pKa = 11.84RR41 pKa = 11.84IGAVPAVRR49 pKa = 11.84TAAGIKK55 pKa = 9.2IVRR58 pKa = 11.84VFDD61 pKa = 3.68TYY63 pKa = 10.98RR64 pKa = 11.84GDD66 pKa = 4.45DD67 pKa = 3.57RR68 pKa = 11.84FHH70 pKa = 6.14QLLRR74 pKa = 11.84NRR76 pKa = 11.84ADD78 pKa = 2.74ISGRR82 pKa = 11.84IRR84 pKa = 11.84KK85 pKa = 8.53II86 pKa = 3.17

Molecular weight:
9.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2148

0

2148

542794

23

1655

252.7

27.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.386 ± 0.072

1.333 ± 0.023

5.505 ± 0.047

6.486 ± 0.06

4.006 ± 0.039

7.872 ± 0.056

2.047 ± 0.025

6.741 ± 0.052

5.88 ± 0.05

9.012 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.026

3.7 ± 0.042

3.796 ± 0.037

2.755 ± 0.029

5.617 ± 0.053

5.199 ± 0.036

5.781 ± 0.047

7.564 ± 0.054

0.87 ± 0.019

3.617 ± 0.035

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski