Caulobacter phage Cr30
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 286 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A067XQP8|A0A067XQP8_9CAUD Uncharacterized protein OS=Caulobacter phage Cr30 OX=1357714 PE=4 SV=1
MM1 pKa = 7.14 TFEE4 pKa = 5.62 DD5 pKa = 4.02 FMKK8 pKa = 10.63 RR9 pKa = 11.84 VDD11 pKa = 3.52 NVIIKK16 pKa = 7.57 EE17 pKa = 4.34 TGLSYY22 pKa = 11.48 LDD24 pKa = 3.84 FCDD27 pKa = 5.32 AQWTDD32 pKa = 2.94 LWEE35 pKa = 4.33 EE36 pKa = 3.92 TDD38 pKa = 4.92 DD39 pKa = 6.42 GEE41 pKa = 4.74 DD42 pKa = 3.85 CSDD45 pKa = 3.47 EE46 pKa = 4.67 SICEE50 pKa = 4.08 TLADD54 pKa = 4.52 ADD56 pKa = 4.43 DD57 pKa = 4.38 IYY59 pKa = 11.83 ARR61 pKa = 11.84 MHH63 pKa = 5.95 YY64 pKa = 10.82 GEE66 pKa = 4.48
Molecular weight: 7.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.63
IPC_protein 3.592
Toseland 3.376
ProMoST 3.745
Dawson 3.592
Bjellqvist 3.808
Wikipedia 3.554
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.935
Thurlkill 3.452
EMBOSS 3.554
Sillero 3.719
Patrickios 0.769
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A067XR89|A0A067XR89_9CAUD Uncharacterized protein OS=Caulobacter phage Cr30 OX=1357714 PE=4 SV=1
MM1 pKa = 7.76 SVGTNRR7 pKa = 11.84 NPVDD11 pKa = 4.03 RR12 pKa = 11.84 NTAAYY17 pKa = 10.65 YY18 pKa = 10.59 LDD20 pKa = 3.66 LVNRR24 pKa = 11.84 YY25 pKa = 8.67 RR26 pKa = 11.84 VMGLKK31 pKa = 9.13 TLEE34 pKa = 4.43 KK35 pKa = 10.25 AAQQQAQRR43 pKa = 11.84 AIRR46 pKa = 11.84 EE47 pKa = 3.97 KK48 pKa = 10.83 RR49 pKa = 11.84 LL50 pKa = 3.24
Molecular weight: 5.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.94
IPC_protein 10.818
Toseland 10.687
ProMoST 10.482
Dawson 10.833
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 10.979
Grimsley 10.891
Solomon 10.95
Lehninger 10.906
Nozaki 10.657
DTASelect 10.584
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.76
Patrickios 10.774
IPC_peptide 10.95
IPC2_peptide 9.516
IPC2.peptide.svr19 8.3
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
286
0
286
48709
37
1201
170.3
19.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.989 ± 0.225
1.027 ± 0.058
6.212 ± 0.131
7.233 ± 0.206
4.903 ± 0.125
5.878 ± 0.181
1.961 ± 0.089
6.921 ± 0.117
7.491 ± 0.257
7.621 ± 0.13
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.23 ± 0.086
5.835 ± 0.145
3.665 ± 0.105
3.708 ± 0.11
4.597 ± 0.127
7.091 ± 0.224
5.709 ± 0.2
6.06 ± 0.144
1.441 ± 0.075
4.43 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here