Amino acid dipepetide frequency for Caulobacter phage Cr30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.311AlaAla: 4.311 ± 0.62
0.595AlaCys: 0.595 ± 0.123
3.49AlaAsp: 3.49 ± 0.327
3.49AlaGlu: 3.49 ± 0.308
2.813AlaPhe: 2.813 ± 0.212
3.511AlaGly: 3.511 ± 0.417
0.924AlaHis: 0.924 ± 0.137
4.64AlaIle: 4.64 ± 0.316
4.845AlaLys: 4.845 ± 0.375
4.722AlaLeu: 4.722 ± 0.4
1.129AlaMet: 1.129 ± 0.166
4.188AlaAsn: 4.188 ± 0.477
1.848AlaPro: 1.848 ± 0.183
2.34AlaGln: 2.34 ± 0.235
2.874AlaArg: 2.874 ± 0.283
4.024AlaSer: 4.024 ± 0.336
3.223AlaThr: 3.223 ± 0.339
3.737AlaVal: 3.737 ± 0.331
0.801AlaTrp: 0.801 ± 0.136
2.382AlaTyr: 2.382 ± 0.255
0.0AlaXaa: 0.0 ± 0.0
Cys
0.657CysAla: 0.657 ± 0.128
0.287CysCys: 0.287 ± 0.074
0.678CysAsp: 0.678 ± 0.132
0.698CysGlu: 0.698 ± 0.132
0.513CysPhe: 0.513 ± 0.098
0.534CysGly: 0.534 ± 0.132
0.308CysHis: 0.308 ± 0.09
0.739CysIle: 0.739 ± 0.133
0.719CysLys: 0.719 ± 0.127
0.76CysLeu: 0.76 ± 0.125
0.205CysMet: 0.205 ± 0.061
0.472CysAsn: 0.472 ± 0.095
0.287CysPro: 0.287 ± 0.085
0.308CysGln: 0.308 ± 0.084
0.39CysArg: 0.39 ± 0.102
0.903CysSer: 0.903 ± 0.165
0.575CysThr: 0.575 ± 0.109
0.678CysVal: 0.678 ± 0.12
0.123CysTrp: 0.123 ± 0.059
0.431CysTyr: 0.431 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
3.901AspAla: 3.901 ± 0.355
0.842AspCys: 0.842 ± 0.126
3.983AspAsp: 3.983 ± 0.342
4.558AspGlu: 4.558 ± 0.281
3.737AspPhe: 3.737 ± 0.277
3.942AspGly: 3.942 ± 0.249
1.314AspHis: 1.314 ± 0.18
4.394AspIle: 4.394 ± 0.308
3.511AspLys: 3.511 ± 0.383
5.051AspLeu: 5.051 ± 0.334
1.088AspMet: 1.088 ± 0.151
2.833AspAsn: 2.833 ± 0.23
2.689AspPro: 2.689 ± 0.258
2.156AspGln: 2.156 ± 0.223
3.039AspArg: 3.039 ± 0.25
4.127AspSer: 4.127 ± 0.358
2.874AspThr: 2.874 ± 0.302
3.695AspVal: 3.695 ± 0.267
1.129AspTrp: 1.129 ± 0.155
3.264AspTyr: 3.264 ± 0.288
0.0AspXaa: 0.0 ± 0.0
Glu
4.27GluAla: 4.27 ± 0.329
0.76GluCys: 0.76 ± 0.144
4.291GluAsp: 4.291 ± 0.28
5.379GluGlu: 5.379 ± 0.409
3.962GluPhe: 3.962 ± 0.355
2.895GluGly: 2.895 ± 0.24
1.519GluHis: 1.519 ± 0.191
6.426GluIle: 6.426 ± 0.331
5.892GluLys: 5.892 ± 0.443
5.728GluLeu: 5.728 ± 0.371
2.382GluMet: 2.382 ± 0.233
4.435GluAsn: 4.435 ± 0.347
1.745GluPro: 1.745 ± 0.23
2.71GluGln: 2.71 ± 0.252
3.326GluArg: 3.326 ± 0.285
3.654GluSer: 3.654 ± 0.238
4.66GluThr: 4.66 ± 0.332
3.572GluVal: 3.572 ± 0.296
1.376GluTrp: 1.376 ± 0.167
3.346GluTyr: 3.346 ± 0.236
0.0GluXaa: 0.0 ± 0.0
Phe
2.772PheAla: 2.772 ± 0.256
0.554PheCys: 0.554 ± 0.105
3.613PheAsp: 3.613 ± 0.232
3.613PheGlu: 3.613 ± 0.307
2.423PhePhe: 2.423 ± 0.263
2.915PheGly: 2.915 ± 0.248
1.129PheHis: 1.129 ± 0.172
2.915PheIle: 2.915 ± 0.275
3.819PheLys: 3.819 ± 0.324
4.147PheLeu: 4.147 ± 0.31
1.109PheMet: 1.109 ± 0.155
2.505PheAsn: 2.505 ± 0.237
1.766PhePro: 1.766 ± 0.163
1.745PheGln: 1.745 ± 0.211
2.197PheArg: 2.197 ± 0.217
3.778PheSer: 3.778 ± 0.298
2.977PheThr: 2.977 ± 0.304
2.895PheVal: 2.895 ± 0.228
0.595PheTrp: 0.595 ± 0.117
1.56PheTyr: 1.56 ± 0.195
0.0PheXaa: 0.0 ± 0.0
Gly
2.977GlyAla: 2.977 ± 0.354
0.678GlyCys: 0.678 ± 0.124
3.511GlyAsp: 3.511 ± 0.278
3.367GlyGlu: 3.367 ± 0.24
3.162GlyPhe: 3.162 ± 0.234
3.531GlyGly: 3.531 ± 0.402
0.903GlyHis: 0.903 ± 0.147
4.209GlyIle: 4.209 ± 0.282
4.578GlyLys: 4.578 ± 0.335
4.003GlyLeu: 4.003 ± 0.279
1.499GlyMet: 1.499 ± 0.207
3.86GlyAsn: 3.86 ± 0.341
1.684GlyPro: 1.684 ± 0.197
2.32GlyGln: 2.32 ± 0.225
2.628GlyArg: 2.628 ± 0.231
4.784GlySer: 4.784 ± 0.431
3.531GlyThr: 3.531 ± 0.303
3.531GlyVal: 3.531 ± 0.348
1.047GlyTrp: 1.047 ± 0.149
2.977GlyTyr: 2.977 ± 0.26
0.0GlyXaa: 0.0 ± 0.0
His
1.006HisAla: 1.006 ± 0.149
0.205HisCys: 0.205 ± 0.064
1.396HisAsp: 1.396 ± 0.207
1.622HisGlu: 1.622 ± 0.193
1.088HisPhe: 1.088 ± 0.168
1.211HisGly: 1.211 ± 0.177
0.698HisHis: 0.698 ± 0.179
1.334HisIle: 1.334 ± 0.187
1.273HisLys: 1.273 ± 0.162
1.663HisLeu: 1.663 ± 0.197
0.513HisMet: 0.513 ± 0.106
0.595HisAsn: 0.595 ± 0.112
0.903HisPro: 0.903 ± 0.151
0.698HisGln: 0.698 ± 0.123
0.719HisArg: 0.719 ± 0.127
1.499HisSer: 1.499 ± 0.211
0.821HisThr: 0.821 ± 0.14
0.924HisVal: 0.924 ± 0.139
0.431HisTrp: 0.431 ± 0.112
1.006HisTyr: 1.006 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
4.496IleAla: 4.496 ± 0.316
0.616IleCys: 0.616 ± 0.124
5.605IleAsp: 5.605 ± 0.327
5.194IleGlu: 5.194 ± 0.347
2.751IlePhe: 2.751 ± 0.199
4.127IleGly: 4.127 ± 0.29
1.684IleHis: 1.684 ± 0.205
3.757IleIle: 3.757 ± 0.307
4.352IleLys: 4.352 ± 0.311
5.317IleLeu: 5.317 ± 0.288
1.027IleMet: 1.027 ± 0.153
3.654IleAsn: 3.654 ± 0.311
3.08IlePro: 3.08 ± 0.268
2.895IleGln: 2.895 ± 0.215
3.819IleArg: 3.819 ± 0.278
5.051IleSer: 5.051 ± 0.354
3.983IleThr: 3.983 ± 0.316
4.743IleVal: 4.743 ± 0.359
0.965IleTrp: 0.965 ± 0.15
2.094IleTyr: 2.094 ± 0.206
0.0IleXaa: 0.0 ± 0.0
Lys
4.086LysAla: 4.086 ± 0.317
0.678LysCys: 0.678 ± 0.121
4.476LysAsp: 4.476 ± 0.405
6.262LysGlu: 6.262 ± 0.39
3.511LysPhe: 3.511 ± 0.277
3.716LysGly: 3.716 ± 0.255
1.766LysHis: 1.766 ± 0.224
5.687LysIle: 5.687 ± 0.36
6.282LysLys: 6.282 ± 0.465
5.461LysLeu: 5.461 ± 0.326
1.909LysMet: 1.909 ± 0.223
4.435LysAsn: 4.435 ± 0.332
2.074LysPro: 2.074 ± 0.205
2.772LysGln: 2.772 ± 0.322
3.203LysArg: 3.203 ± 0.327
4.845LysSer: 4.845 ± 0.32
3.983LysThr: 3.983 ± 0.275
4.496LysVal: 4.496 ± 0.273
0.903LysTrp: 0.903 ± 0.143
4.373LysTyr: 4.373 ± 0.339
0.0LysXaa: 0.0 ± 0.0
Leu
4.599LeuAla: 4.599 ± 0.298
0.636LeuCys: 0.636 ± 0.116
4.64LeuAsp: 4.64 ± 0.303
6.426LeuGlu: 6.426 ± 0.45
3.367LeuPhe: 3.367 ± 0.234
4.414LeuGly: 4.414 ± 0.26
1.478LeuHis: 1.478 ± 0.183
4.394LeuIle: 4.394 ± 0.36
6.755LeuLys: 6.755 ± 0.401
5.153LeuLeu: 5.153 ± 0.365
1.663LeuMet: 1.663 ± 0.214
4.907LeuAsn: 4.907 ± 0.316
2.956LeuPro: 2.956 ± 0.268
3.059LeuGln: 3.059 ± 0.245
3.757LeuArg: 3.757 ± 0.257
5.749LeuSer: 5.749 ± 0.362
4.25LeuThr: 4.25 ± 0.32
3.942LeuVal: 3.942 ± 0.237
0.719LeuTrp: 0.719 ± 0.131
3.346LeuTyr: 3.346 ± 0.252
0.0LeuXaa: 0.0 ± 0.0
Met
1.601MetAla: 1.601 ± 0.19
0.287MetCys: 0.287 ± 0.068
0.944MetAsp: 0.944 ± 0.151
1.355MetGlu: 1.355 ± 0.176
1.088MetPhe: 1.088 ± 0.145
0.944MetGly: 0.944 ± 0.144
0.226MetHis: 0.226 ± 0.065
1.745MetIle: 1.745 ± 0.197
2.217MetLys: 2.217 ± 0.233
1.704MetLeu: 1.704 ± 0.178
0.616MetMet: 0.616 ± 0.107
1.601MetAsn: 1.601 ± 0.215
0.842MetPro: 0.842 ± 0.15
0.698MetGln: 0.698 ± 0.124
0.985MetArg: 0.985 ± 0.157
1.827MetSer: 1.827 ± 0.212
1.622MetThr: 1.622 ± 0.165
1.417MetVal: 1.417 ± 0.171
0.164MetTrp: 0.164 ± 0.067
0.411MetTyr: 0.411 ± 0.088
0.0MetXaa: 0.0 ± 0.0
Asn
3.983AsnAla: 3.983 ± 0.355
0.636AsnCys: 0.636 ± 0.129
3.121AsnAsp: 3.121 ± 0.265
3.737AsnGlu: 3.737 ± 0.325
2.34AsnPhe: 2.34 ± 0.194
4.168AsnGly: 4.168 ± 0.377
1.355AsnHis: 1.355 ± 0.189
3.921AsnIle: 3.921 ± 0.31
2.854AsnLys: 2.854 ± 0.223
4.701AsnLeu: 4.701 ± 0.352
1.006AsnMet: 1.006 ± 0.127
2.874AsnAsn: 2.874 ± 0.283
2.731AsnPro: 2.731 ± 0.196
2.484AsnGln: 2.484 ± 0.207
2.689AsnArg: 2.689 ± 0.267
4.086AsnSer: 4.086 ± 0.467
3.962AsnThr: 3.962 ± 0.605
4.352AsnVal: 4.352 ± 0.437
0.821AsnTrp: 0.821 ± 0.149
2.525AsnTyr: 2.525 ± 0.232
0.0AsnXaa: 0.0 ± 0.0
Pro
1.642ProAla: 1.642 ± 0.23
0.308ProCys: 0.308 ± 0.079
2.279ProAsp: 2.279 ± 0.212
3.244ProGlu: 3.244 ± 0.31
1.848ProPhe: 1.848 ± 0.204
2.546ProGly: 2.546 ± 0.22
0.595ProHis: 0.595 ± 0.097
2.628ProIle: 2.628 ± 0.227
2.607ProLys: 2.607 ± 0.27
2.074ProLeu: 2.074 ± 0.232
0.78ProMet: 0.78 ± 0.126
2.32ProAsn: 2.32 ± 0.216
1.273ProPro: 1.273 ± 0.182
1.334ProGln: 1.334 ± 0.163
1.684ProArg: 1.684 ± 0.185
2.546ProSer: 2.546 ± 0.248
1.909ProThr: 1.909 ± 0.219
2.566ProVal: 2.566 ± 0.239
0.678ProTrp: 0.678 ± 0.116
1.786ProTyr: 1.786 ± 0.179
0.0ProXaa: 0.0 ± 0.0
Gln
2.135GlnAla: 2.135 ± 0.211
0.328GlnCys: 0.328 ± 0.086
2.402GlnAsp: 2.402 ± 0.228
3.244GlnGlu: 3.244 ± 0.331
2.197GlnPhe: 2.197 ± 0.185
2.156GlnGly: 2.156 ± 0.204
0.554GlnHis: 0.554 ± 0.116
2.731GlnIle: 2.731 ± 0.21
2.997GlnLys: 2.997 ± 0.253
2.566GlnLeu: 2.566 ± 0.249
1.129GlnMet: 1.129 ± 0.144
2.505GlnAsn: 2.505 ± 0.238
1.211GlnPro: 1.211 ± 0.155
1.088GlnGln: 1.088 ± 0.16
1.499GlnArg: 1.499 ± 0.173
1.93GlnSer: 1.93 ± 0.201
1.971GlnThr: 1.971 ± 0.219
1.786GlnVal: 1.786 ± 0.175
0.595GlnTrp: 0.595 ± 0.126
2.053GlnTyr: 2.053 ± 0.23
0.0GlnXaa: 0.0 ± 0.0
Arg
2.648ArgAla: 2.648 ± 0.233
0.411ArgCys: 0.411 ± 0.095
2.382ArgAsp: 2.382 ± 0.242
3.49ArgGlu: 3.49 ± 0.307
1.95ArgPhe: 1.95 ± 0.197
2.299ArgGly: 2.299 ± 0.221
0.719ArgHis: 0.719 ± 0.136
3.47ArgIle: 3.47 ± 0.268
4.743ArgLys: 4.743 ± 0.409
3.798ArgLeu: 3.798 ± 0.278
1.376ArgMet: 1.376 ± 0.197
3.346ArgAsn: 3.346 ± 0.259
1.478ArgPro: 1.478 ± 0.168
1.355ArgGln: 1.355 ± 0.16
2.217ArgArg: 2.217 ± 0.237
2.34ArgSer: 2.34 ± 0.231
1.991ArgThr: 1.991 ± 0.184
2.772ArgVal: 2.772 ± 0.208
0.78ArgTrp: 0.78 ± 0.116
2.382ArgTyr: 2.382 ± 0.213
0.0ArgXaa: 0.0 ± 0.0
Ser
3.675SerAla: 3.675 ± 0.343
0.554SerCys: 0.554 ± 0.109
3.86SerAsp: 3.86 ± 0.282
5.174SerGlu: 5.174 ± 0.305
3.593SerPhe: 3.593 ± 0.298
4.948SerGly: 4.948 ± 0.567
1.232SerHis: 1.232 ± 0.143
5.256SerIle: 5.256 ± 0.324
5.707SerLys: 5.707 ± 0.403
5.215SerLeu: 5.215 ± 0.357
1.54SerMet: 1.54 ± 0.169
4.517SerAsn: 4.517 ± 0.587
2.115SerPro: 2.115 ± 0.205
2.217SerGln: 2.217 ± 0.196
2.689SerArg: 2.689 ± 0.238
6.303SerSer: 6.303 ± 0.514
4.127SerThr: 4.127 ± 0.439
4.476SerVal: 4.476 ± 0.469
0.862SerTrp: 0.862 ± 0.134
2.854SerTyr: 2.854 ± 0.288
0.0SerXaa: 0.0 ± 0.0
Thr
3.511ThrAla: 3.511 ± 0.375
0.37ThrCys: 0.37 ± 0.082
3.162ThrAsp: 3.162 ± 0.249
3.367ThrGlu: 3.367 ± 0.27
3.08ThrPhe: 3.08 ± 0.28
3.88ThrGly: 3.88 ± 0.405
0.842ThrHis: 0.842 ± 0.136
4.25ThrIle: 4.25 ± 0.369
3.593ThrLys: 3.593 ± 0.288
4.989ThrLeu: 4.989 ± 0.355
0.985ThrMet: 0.985 ± 0.139
2.607ThrAsn: 2.607 ± 0.289
3.305ThrPro: 3.305 ± 0.243
1.766ThrGln: 1.766 ± 0.19
2.238ThrArg: 2.238 ± 0.231
4.435ThrSer: 4.435 ± 0.501
4.147ThrThr: 4.147 ± 0.439
3.654ThrVal: 3.654 ± 0.302
0.821ThrTrp: 0.821 ± 0.129
2.094ThrTyr: 2.094 ± 0.226
0.0ThrXaa: 0.0 ± 0.0
Val
4.106ValAla: 4.106 ± 0.374
0.678ValCys: 0.678 ± 0.135
4.045ValAsp: 4.045 ± 0.283
4.619ValGlu: 4.619 ± 0.329
2.833ValPhe: 2.833 ± 0.259
3.305ValGly: 3.305 ± 0.301
0.985ValHis: 0.985 ± 0.135
3.346ValIle: 3.346 ± 0.306
4.229ValLys: 4.229 ± 0.302
4.578ValLeu: 4.578 ± 0.322
1.027ValMet: 1.027 ± 0.141
3.367ValAsn: 3.367 ± 0.308
2.464ValPro: 2.464 ± 0.264
2.546ValGln: 2.546 ± 0.288
3.08ValArg: 3.08 ± 0.224
4.968ValSer: 4.968 ± 0.521
3.47ValThr: 3.47 ± 0.327
3.86ValVal: 3.86 ± 0.376
0.575ValTrp: 0.575 ± 0.112
2.525ValTyr: 2.525 ± 0.225
0.0ValXaa: 0.0 ± 0.0
Trp
0.739TrpAla: 0.739 ± 0.094
0.185TrpCys: 0.185 ± 0.062
0.78TrpAsp: 0.78 ± 0.113
0.924TrpGlu: 0.924 ± 0.14
0.636TrpPhe: 0.636 ± 0.119
0.719TrpGly: 0.719 ± 0.126
0.267TrpHis: 0.267 ± 0.097
1.027TrpIle: 1.027 ± 0.176
1.478TrpLys: 1.478 ± 0.227
0.985TrpLeu: 0.985 ± 0.17
0.411TrpMet: 0.411 ± 0.078
1.15TrpAsn: 1.15 ± 0.151
0.452TrpPro: 0.452 ± 0.102
0.513TrpGln: 0.513 ± 0.095
0.801TrpArg: 0.801 ± 0.131
0.924TrpSer: 0.924 ± 0.116
0.616TrpThr: 0.616 ± 0.114
0.76TrpVal: 0.76 ± 0.121
0.267TrpTrp: 0.267 ± 0.081
0.78TrpTyr: 0.78 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.772TyrAla: 2.772 ± 0.279
0.657TyrCys: 0.657 ± 0.121
3.47TyrAsp: 3.47 ± 0.258
2.566TyrGlu: 2.566 ± 0.195
2.135TyrPhe: 2.135 ± 0.194
3.018TyrGly: 3.018 ± 0.257
1.109TyrHis: 1.109 ± 0.16
2.34TyrIle: 2.34 ± 0.223
2.464TyrLys: 2.464 ± 0.268
3.593TyrLeu: 3.593 ± 0.249
0.883TyrMet: 0.883 ± 0.167
2.176TyrAsn: 2.176 ± 0.196
1.745TyrPro: 1.745 ± 0.178
2.074TyrGln: 2.074 ± 0.221
2.135TyrArg: 2.135 ± 0.224
3.141TyrSer: 3.141 ± 0.277
2.402TyrThr: 2.402 ± 0.291
2.751TyrVal: 2.751 ± 0.274
0.76TyrTrp: 0.76 ± 0.125
2.115TyrTyr: 2.115 ± 0.255
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 286 proteins (48709 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski