Tolypocladium paradoxum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Tolypocladium

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8979 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S4KU88|A0A2S4KU88_9HYPO Matrin-type domain-containing protein OS=Tolypocladium paradoxum OX=94208 GN=TPAR_06016 PE=3 SV=1
MM1 pKa = 6.73STKK4 pKa = 9.85ILYY7 pKa = 9.86DD8 pKa = 3.43AVVGPGANEE17 pKa = 3.91WYY19 pKa = 7.75GTLVVTNIRR28 pKa = 11.84YY29 pKa = 9.92DD30 pKa = 3.21GGGTVTVQKK39 pKa = 10.63FLGVEE44 pKa = 4.34FLSPATVAATDD55 pKa = 3.74INVLTNPWVQVTPEE69 pKa = 4.0VTNEE73 pKa = 4.08QIDD76 pKa = 3.73SSTFSITAKK85 pKa = 10.58LNVDD89 pKa = 3.37NSVSLTGTTITIGINGDD106 pKa = 3.48LTKK109 pKa = 11.06DD110 pKa = 3.0SDD112 pKa = 3.9RR113 pKa = 11.84YY114 pKa = 6.25TTSFVFVADD123 pKa = 5.43GIPDD127 pKa = 3.41TSGTVNVDD135 pKa = 3.7CAASPDD141 pKa = 3.8PALDD145 pKa = 4.0GEE147 pKa = 4.48QQAVTFTLGGRR158 pKa = 11.84VVNVTVPLGEE168 pKa = 4.1TTPAKK173 pKa = 10.57VPAGTYY179 pKa = 10.44DD180 pKa = 3.22VTAAEE185 pKa = 4.8LTTDD189 pKa = 3.18AQTVVASAQVSPTSVTVVAGEE210 pKa = 4.27TVDD213 pKa = 3.9VAVTYY218 pKa = 10.85DD219 pKa = 3.62LVEE222 pKa = 4.56KK223 pKa = 10.84YY224 pKa = 10.55SAIDD228 pKa = 3.48VTIGTILPLEE238 pKa = 4.44KK239 pKa = 10.45EE240 pKa = 4.15QFHH243 pKa = 5.72VQVVDD248 pKa = 3.56QGSGTILADD257 pKa = 4.29FSSPINQTTSLRR269 pKa = 11.84RR270 pKa = 11.84LPASGTAVVSVDD282 pKa = 2.9GTTLNNVEE290 pKa = 3.87YY291 pKa = 10.39SFAVKK296 pKa = 10.51SEE298 pKa = 4.2DD299 pKa = 3.58LSPSLFQVSFSQDD312 pKa = 3.21DD313 pKa = 4.0VTTTPVDD320 pKa = 3.27TTGFVTLPIVVGTDD334 pKa = 2.69ITLDD338 pKa = 3.23ATISVRR344 pKa = 11.84LTSSTNYY351 pKa = 10.14VYY353 pKa = 10.09TQDD356 pKa = 3.28VKK358 pKa = 11.22AAAGATNFAVPVGPGYY374 pKa = 9.36YY375 pKa = 8.77TVQAASIINNGIVYY389 pKa = 9.28VVSISATSLTVKK401 pKa = 10.57ADD403 pKa = 3.44GSSTVQLTLQRR414 pKa = 11.84GANLNVRR421 pKa = 11.84GFPGFLSFGGCADD434 pKa = 3.74LTSGNQADD442 pKa = 4.51FAAALASSVFKK453 pKa = 11.25YY454 pKa = 10.58AGTDD458 pKa = 3.5GAGDD462 pKa = 3.71AGSFLTDD469 pKa = 3.52DD470 pKa = 3.17QATRR474 pKa = 11.84QTIALARR481 pKa = 11.84AVEE484 pKa = 4.21TQVADD489 pKa = 4.08GNPVLPVMISYY500 pKa = 7.82TCNMSGGNPADD511 pKa = 3.56HH512 pKa = 6.54LQNVDD517 pKa = 3.35GLAHH521 pKa = 6.57SFANLILSMNIATATIDD538 pKa = 3.68DD539 pKa = 4.11QHH541 pKa = 6.7PVPAGYY547 pKa = 9.93VVNPDD552 pKa = 4.79FIGACQQDD560 pKa = 4.5GLSADD565 pKa = 3.45YY566 pKa = 11.3AMQVRR571 pKa = 11.84APLTTALDD579 pKa = 3.49HH580 pKa = 6.59WSVSAEE586 pKa = 3.8IPSTITEE593 pKa = 4.17DD594 pKa = 3.19LRR596 pKa = 11.84GYY598 pKa = 9.93VLAVNWLIRR607 pKa = 11.84TVAPAVTFGWQVNLWGVGASTWVYY631 pKa = 11.01QDD633 pKa = 5.21DD634 pKa = 4.27DD635 pKa = 3.79VADD638 pKa = 3.87IARR641 pKa = 11.84QTGDD645 pKa = 3.23YY646 pKa = 10.76AKK648 pKa = 9.85TLGVFDD654 pKa = 5.26GDD656 pKa = 3.83NLPDD660 pKa = 4.05FLAVDD665 pKa = 4.68RR666 pKa = 11.84YY667 pKa = 10.05EE668 pKa = 5.88ADD670 pKa = 3.55DD671 pKa = 3.82FTVRR675 pKa = 11.84AYY677 pKa = 10.91GNGYY681 pKa = 10.25CYY683 pKa = 10.58GPRR686 pKa = 11.84EE687 pKa = 3.94WGRR690 pKa = 11.84FFDD693 pKa = 4.58FCAALSAQLQFPIMPWQIPSSWTPLTTDD721 pKa = 3.21AVNDD725 pKa = 3.86NFDD728 pKa = 3.65SQHH731 pKa = 6.01WGTAGSYY738 pKa = 10.86IMGDD742 pKa = 3.33PGIGSDD748 pKa = 3.68YY749 pKa = 11.11HH750 pKa = 6.62NVNPKK755 pKa = 9.64ILALQFSPALPYY767 pKa = 9.87MGSTAEE773 pKa = 4.81DD774 pKa = 3.33IFTRR778 pKa = 11.84AEE780 pKa = 4.14PFDD783 pKa = 4.24LTSPAYY789 pKa = 10.6SDD791 pKa = 3.71FPLRR795 pKa = 11.84GIFTVLLGGGSTTGIVSSIGNPQPWVRR822 pKa = 11.84DD823 pKa = 3.33KK824 pKa = 11.41LYY826 pKa = 10.49TYY828 pKa = 7.47MQNSISFNDD837 pKa = 3.36

Molecular weight: 88.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S4L963|A0A2S4L963_9HYPO Uncharacterized protein (Fragment) OS=Tolypocladium paradoxum OX=94208 GN=TPAR_00843 PE=4 SV=1
PP1 pKa = 7.28LGRR4 pKa = 11.84ARR6 pKa = 11.84RR7 pKa = 11.84HH8 pKa = 3.97QRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84ALRR15 pKa = 11.84PEE17 pKa = 4.07PQPGRR22 pKa = 11.84RR23 pKa = 11.84LPRR26 pKa = 11.84VRR28 pKa = 11.84HH29 pKa = 5.65HH30 pKa = 6.87RR31 pKa = 11.84RR32 pKa = 11.84AAPARR37 pKa = 11.84PLRR40 pKa = 11.84GAAPRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84PHH50 pKa = 5.27RR51 pKa = 11.84QPRR54 pKa = 11.84RR55 pKa = 11.84QRR57 pKa = 11.84RR58 pKa = 11.84EE59 pKa = 3.09RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84PRR64 pKa = 11.84RR65 pKa = 11.84LQARR69 pKa = 11.84QRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84HH74 pKa = 4.56RR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84LPGHH84 pKa = 6.55RR85 pKa = 11.84RR86 pKa = 11.84QGPRR90 pKa = 11.84VHH92 pKa = 6.71EE93 pKa = 4.41LGEE96 pKa = 4.02RR97 pKa = 11.84RR98 pKa = 11.84QRR100 pKa = 11.84QPDD103 pKa = 3.71GPAEE107 pKa = 4.19CRR109 pKa = 11.84RR110 pKa = 11.84AVAGRR115 pKa = 11.84SRR117 pKa = 11.84GAAARR122 pKa = 11.84VLLGDD127 pKa = 3.24QGRR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 11.84AARR135 pKa = 11.84PRR137 pKa = 11.84RR138 pKa = 11.84QPPVPLQPPHH148 pKa = 6.66HH149 pKa = 6.79RR150 pKa = 11.84PPPRR154 pKa = 11.84RR155 pKa = 11.84HH156 pKa = 5.43LRR158 pKa = 11.84RR159 pKa = 11.84GRR161 pKa = 11.84RR162 pKa = 11.84PGPRR166 pKa = 11.84RR167 pKa = 11.84PAARR171 pKa = 11.84PPLQPDD177 pKa = 2.97QRR179 pKa = 11.84PARR182 pKa = 11.84RR183 pKa = 11.84QPQHH187 pKa = 6.31LRR189 pKa = 11.84LHH191 pKa = 6.83LRR193 pKa = 11.84RR194 pKa = 11.84QRR196 pKa = 11.84RR197 pKa = 11.84PRR199 pKa = 11.84PPPRR203 pKa = 11.84RR204 pKa = 11.84PRR206 pKa = 11.84PPRR209 pKa = 11.84RR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84RR213 pKa = 11.84LRR215 pKa = 11.84PAPRR219 pKa = 11.84PPKK222 pKa = 9.63GRR224 pKa = 11.84RR225 pKa = 11.84RR226 pKa = 11.84ALLRR230 pKa = 11.84HH231 pKa = 5.92KK232 pKa = 10.47RR233 pKa = 11.84RR234 pKa = 11.84ALLLLGLCPRR244 pKa = 11.84RR245 pKa = 11.84LARR248 pKa = 11.84RR249 pKa = 11.84RR250 pKa = 11.84PRR252 pKa = 11.84ARR254 pKa = 11.84HH255 pKa = 5.29QGRR258 pKa = 11.84LDD260 pKa = 3.51PPPRR264 pKa = 11.84PRR266 pKa = 11.84HH267 pKa = 4.73LHH269 pKa = 5.75GLLQRR274 pKa = 11.84GLQQHH279 pKa = 6.29HH280 pKa = 6.19RR281 pKa = 11.84QAPHH285 pKa = 7.28LYY287 pKa = 9.28PPARR291 pKa = 11.84RR292 pKa = 11.84HH293 pKa = 4.66GHH295 pKa = 6.31HH296 pKa = 7.47DD297 pKa = 3.46ALLQHH302 pKa = 6.53HH303 pKa = 6.5QGQEE307 pKa = 3.89RR308 pKa = 11.84PALRR312 pKa = 11.84AAVDD316 pKa = 3.7PAGGRR321 pKa = 11.84HH322 pKa = 4.92PRR324 pKa = 11.84RR325 pKa = 11.84RR326 pKa = 11.84LVPGAGEE333 pKa = 3.99QGPHH337 pKa = 5.46QGAII341 pKa = 3.55

Molecular weight: 40.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8912

67

8979

4469941

8

12706

497.8

54.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.653 ± 0.025

1.29 ± 0.008

5.913 ± 0.02

5.879 ± 0.025

3.512 ± 0.015

7.269 ± 0.023

2.481 ± 0.012

4.208 ± 0.018

4.487 ± 0.022

8.828 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.01

3.257 ± 0.013

6.364 ± 0.026

3.998 ± 0.017

6.816 ± 0.025

7.958 ± 0.026

5.587 ± 0.017

6.293 ± 0.022

1.434 ± 0.009

2.503 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski