Neomonachus schauinslandi (Hawaiian monk seal) (Monachus schauinslandi)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora; <

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Y9HK73|A0A2Y9HK73_NEOSC Signal peptidase complex subunit 3 OS=Neomonachus schauinslandi OX=29088 GN=SPCS3 PE=3 SV=1
MM1 pKa = 6.98VVVLRR6 pKa = 11.84ISAMVPEE13 pKa = 4.73LAVAVVPEE21 pKa = 5.21DD22 pKa = 3.35ISDD25 pKa = 3.72GARR28 pKa = 11.84AGRR31 pKa = 11.84GGGAEE36 pKa = 4.61GISNGAGAGCGGGGQDD52 pKa = 3.33ISDD55 pKa = 3.94GAGVGHH61 pKa = 7.05GSGAEE66 pKa = 4.2DD67 pKa = 4.34IIDD70 pKa = 3.86SAGTGYY76 pKa = 11.05GGGGEE81 pKa = 4.95DD82 pKa = 4.97ISDD85 pKa = 3.61SSCFGRR91 pKa = 11.84GGGNISDD98 pKa = 4.47CAGAGCGGGG107 pKa = 3.36

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Y9HZA8|A0A2Y9HZA8_NEOSC calcium/calmodulin-dependent protein kinase type IV OS=Neomonachus schauinslandi OX=29088 GN=CAMK4 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18709

7510

26219

15552255

31

34351

593.2

66.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.133 ± 0.018

2.171 ± 0.014

4.846 ± 0.01

7.174 ± 0.021

3.621 ± 0.01

6.652 ± 0.022

2.563 ± 0.007

4.276 ± 0.014

5.677 ± 0.019

9.923 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.007

3.547 ± 0.011

6.409 ± 0.026

4.764 ± 0.014

5.819 ± 0.014

8.249 ± 0.021

5.191 ± 0.012

5.995 ± 0.015

1.192 ± 0.006

2.636 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski